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The map label for this gene is menH [H]

Identifier: 157963783

GI number: 157963783

Start: 4826500

End: 4827285

Strand: Direct

Name: menH [H]

Synonym: Spea_3972

Alternate gene names: 157963783

Gene position: 4826500-4827285 (Clockwise)

Preceding gene: 157963782

Following gene: 157963784

Centisome position: 93.27

GC content: 48.85

Gene sequence:

>786_bases
ATGCTAAGCTATCGCACCGCAGGGAAAGCTAGCTCTCCAGCCGTGGTATTTCTCCACGGCTTCTTAGGCAGTAAAGAAGA
TTGGCATGAGATCATCGAGCAGCTTCAAGATGATTTTTACTGCATTAGTCTCGACCTTCCCGGCCATGGCGATAACAGCG
CGCTCAGCCTCGATGAACAGTACCTAGCCACGCCAGGGTTTAGCCGCTGTGCAGAACTCATTAACACTAGGCTATCGTCG
CTTAATATTAAACATTATCACTTGGTTGGCTATTCACTAGGTGGCCGCATTGCGCTGCATATCGCCAAGTTATATCCGCA
GCAGTTATTAAGTTTAGTATTAGAGTCCTGCCACCCGGGGCTACACAGCGAGGCAGACAAGTTAGCCCGCAAGCAAAACG
ATGCAGCTTGGGCTAATAAGCTCAGTACTCTGCCAATGAGTGATTTTTTGCGCCTATGGTATCAGCAGGCGGTATTCAGT
GATTTAACCGCCGCAGAGCAACACGCCTTAGTACTTAAGCGGTCCAGCAATAACCCGTTAGCATTGGCGAATTGCTACCA
AGCAACGTCGCTGGCAGAGCAAGATGATCTTTGGGATATTCCCACTCGATTAAAGGTGAATAGCCACTTTATTGCCGGGC
ATCAAGACAGTAAATTTATGGCGTTAGCAAAGCGCTGGCAGCAACAACAGCCCTTATCTTTGCACTGTATTCACGCCAGT
GGTCACAATGTGCATTTAGCCGCACCGCAAGCATTCGCCATTAAATTGCGCGCGCTTTTCAGTTAG

Upstream 100 bases:

>100_bases
CCATGACCTATTCAGGCCCCTCGGTTATTGAGGTTACTGTGGCACAAGACCAAGCCAGTGAGCAGATAGCTCAAATAGCT
AGCTGGGTAAAACAGAGCTA

Downstream 100 bases:

>100_bases
CATATATTCGCTAGAACTCATTACTGATATAACAAATAACCATGCAGGCAGGCCAATGACAACGACGCAACAGCAAGCAA
CGCCATTTACTCTAACGCAC

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLSYRTAGKASSPAVVFLHGFLGSKEDWHEIIEQLQDDFYCISLDLPGHGDNSALSLDEQYLATPGFSRCAELINTRLSS
LNIKHYHLVGYSLGGRIALHIAKLYPQQLLSLVLESCHPGLHSEADKLARKQNDAAWANKLSTLPMSDFLRLWYQQAVFS
DLTAAEQHALVLKRSSNNPLALANCYQATSLAEQDDLWDIPTRLKVNSHFIAGHQDSKFMALAKRWQQQQPLSLHCIHAS
GHNVHLAAPQAFAIKLRALFS

Sequences:

>Translated_261_residues
MLSYRTAGKASSPAVVFLHGFLGSKEDWHEIIEQLQDDFYCISLDLPGHGDNSALSLDEQYLATPGFSRCAELINTRLSS
LNIKHYHLVGYSLGGRIALHIAKLYPQQLLSLVLESCHPGLHSEADKLARKQNDAAWANKLSTLPMSDFLRLWYQQAVFS
DLTAAEQHALVLKRSSNNPLALANCYQATSLAEQDDLWDIPTRLKVNSHFIAGHQDSKFMALAKRWQQQQPLSLHCIHAS
GHNVHLAAPQAFAIKLRALFS
>Mature_261_residues
MLSYRTAGKASSPAVVFLHGFLGSKEDWHEIIEQLQDDFYCISLDLPGHGDNSALSLDEQYLATPGFSRCAELINTRLSS
LNIKHYHLVGYSLGGRIALHIAKLYPQQLLSLVLESCHPGLHSEADKLARKQNDAAWANKLSTLPMSDFLRLWYQQAVFS
DLTAAEQHALVLKRSSNNPLALANCYQATSLAEQDDLWDIPTRLKVNSHFIAGHQDSKFMALAKRWQQQQPLSLHCIHAS
GHNVHLAAPQAFAIKLRALFS

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Homo sapiens, GI189027141, Length=259, Percent_Identity=27.7992277992278, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1788598, Length=242, Percent_Identity=37.1900826446281, Blast_Score=151, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 29122; Mature: 29122

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSYRTAGKASSPAVVFLHGFLGSKEDWHEIIEQLQDDFYCISLDLPGHGDNSALSLDEQ
CCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCHH
YLATPGFSRCAELINTRLSSLNIKHYHLVGYSLGGRIALHIAKLYPQQLLSLVLESCHPG
HHCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LHSEADKLARKQNDAAWANKLSTLPMSDFLRLWYQQAVFSDLTAAEQHALVLKRSSNNPL
CCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCE
ALANCYQATSLAEQDDLWDIPTRLKVNSHFIAGHQDSKFMALAKRWQQQQPLSLHCIHAS
EEEHHHHHHHCCCCCCCCCCCCEEEECCEEEECCCCCHHHHHHHHHHHCCCCEEEEEECC
GHNVHLAAPQAFAIKLRALFS
CCEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure
MLSYRTAGKASSPAVVFLHGFLGSKEDWHEIIEQLQDDFYCISLDLPGHGDNSALSLDEQ
CCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCHH
YLATPGFSRCAELINTRLSSLNIKHYHLVGYSLGGRIALHIAKLYPQQLLSLVLESCHPG
HHCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LHSEADKLARKQNDAAWANKLSTLPMSDFLRLWYQQAVFSDLTAAEQHALVLKRSSNNPL
CCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCE
ALANCYQATSLAEQDDLWDIPTRLKVNSHFIAGHQDSKFMALAKRWQQQQPLSLHCIHAS
EEEHHHHHHHCCCCCCCCCCCCEEEECCEEEECCCCCHHHHHHHHHHHCCCCEEEEEECC
GHNVHLAAPQAFAIKLRALFS
CCEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA