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The map label for this gene is nrfD [H]

Identifier: 157963776

GI number: 157963776

Start: 4819335

End: 4820276

Strand: Reverse

Name: nrfD [H]

Synonym: Spea_3965

Alternate gene names: 157963776

Gene position: 4820276-4819335 (Counterclockwise)

Preceding gene: 157963777

Following gene: 157963775

Centisome position: 93.15

GC content: 45.33

Gene sequence:

>942_bases
ATGAGTCCATTCCATTTTGACAGCCTAACTTGGCCATGGCCAATTGCCATCTACCTATTTCTTGCGGGTATATCGGCCGG
AGCCATCTGCTTTGCTGTATTGCTAAAACACTTTAAATTGGGACGTGATGCCTATAAATCAGGCTTTGTCCAGGCGGCTT
GTCTTATTGCACCGTTGGCTGTATTTGCAGGGCTTGGCATTTTAGTTATCGATTTAACTAAGCCGTTCGATTTTTGGAAG
ATCTTAGTGTTCTACAACCCGACTTCTGTGATGTCTGTTGGTGTCGCTGTACTGATGGTCTATCAGGTTGCACTGTTCGC
TTGGTTAGCTTGTGTCTTTAAAGAGCCTGCGCTTTCTTGGTGTAACGGTCGCTGGCCAGTTGTTGAGAAGTTATTTAGTG
CAATCGCTAAGCATGAAGCAAGCATTACAGGCTTCTTGGTGATCCTTGCCTTGTCATTAGGTGCATATACTGGCTTCCTG
CTATCGGCATTAACGACTTATCCAATGCTAAATAATCCGGTATTGCCTTTGCTGTTTTTGGTCTCAGGGCTTTCATCGGG
AGCTGCGGGGACTCTACTCGGCGGCGTATTGCTCAAAGGTAATCCTGATGGTAAGGAAGTGCACTTTATCCACAATATTG
AGATCCCAATGATTTTGGTGGAAATTGTGCTTATCTTCACCTTCTTTACAGGCTTGATTTTAACTGGAGGCCAGAGCCAA
GTGGCTGCATTTGCTGCAATTGGAGAAGGCTTCTGGGGCTGGATATTCTGGGCTGGTATCGTTGTTGTTGGTTTGAGTAT
GCCACTGGCCTTTAACCTATTTATGAAAGCCTCCTCTAAGCGCAAGTTCTCTTATGTGGCGATTACTGCCAGCTGCAGTT
TAATCGGCGTACTTTTACTGCGTAACTTCATTCTTTATACCGGCCAAATGACGGTTGCTTAA

Upstream 100 bases:

>100_bases
TACAAGTGCTTAAATCAGCCCCTAGCTACCGAACTAAAACTGATCTCGGGACCAGGCCTAAGTTGTTCCGTATTGCATCA
CAAAGTGGGGAGATAAAACT

Downstream 100 bases:

>100_bases
TTATTTACTGAGGGTTTGAACATGCTTAAAGTTTTAGGATTAACAGTTTTATTGAGCTTTTCGGGTGTAGTGATGGCAAC
TTCAGTTGCTGACTCACATA

Product: polysulfide reductase NrfD

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSPFHFDSLTWPWPIAIYLFLAGISAGAICFAVLLKHFKLGRDAYKSGFVQAACLIAPLAVFAGLGILVIDLTKPFDFWK
ILVFYNPTSVMSVGVAVLMVYQVALFAWLACVFKEPALSWCNGRWPVVEKLFSAIAKHEASITGFLVILALSLGAYTGFL
LSALTTYPMLNNPVLPLLFLVSGLSSGAAGTLLGGVLLKGNPDGKEVHFIHNIEIPMILVEIVLIFTFFTGLILTGGQSQ
VAAFAAIGEGFWGWIFWAGIVVVGLSMPLAFNLFMKASSKRKFSYVAITASCSLIGVLLLRNFILYTGQMTVA

Sequences:

>Translated_313_residues
MSPFHFDSLTWPWPIAIYLFLAGISAGAICFAVLLKHFKLGRDAYKSGFVQAACLIAPLAVFAGLGILVIDLTKPFDFWK
ILVFYNPTSVMSVGVAVLMVYQVALFAWLACVFKEPALSWCNGRWPVVEKLFSAIAKHEASITGFLVILALSLGAYTGFL
LSALTTYPMLNNPVLPLLFLVSGLSSGAAGTLLGGVLLKGNPDGKEVHFIHNIEIPMILVEIVLIFTFFTGLILTGGQSQ
VAAFAAIGEGFWGWIFWAGIVVVGLSMPLAFNLFMKASSKRKFSYVAITASCSLIGVLLLRNFILYTGQMTVA
>Mature_312_residues
SPFHFDSLTWPWPIAIYLFLAGISAGAICFAVLLKHFKLGRDAYKSGFVQAACLIAPLAVFAGLGILVIDLTKPFDFWKI
LVFYNPTSVMSVGVAVLMVYQVALFAWLACVFKEPALSWCNGRWPVVEKLFSAIAKHEASITGFLVILALSLGAYTGFLL
SALTTYPMLNNPVLPLLFLVSGLSSGAAGTLLGGVLLKGNPDGKEVHFIHNIEIPMILVEIVLIFTFFTGLILTGGQSQV
AAFAAIGEGFWGWIFWAGIVVVGLSMPLAFNLFMKASSKRKFSYVAITASCSLIGVLLLRNFILYTGQMTVA

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=319, Percent_Identity=45.7680250783699, Blast_Score=252, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 33863; Mature: 33732

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPFHFDSLTWPWPIAIYLFLAGISAGAICFAVLLKHFKLGRDAYKSGFVQAACLIAPLA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFAGLGILVIDLTKPFDFWKILVFYNPTSVMSVGVAVLMVYQVALFAWLACVFKEPALSW
HHHCCCEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
CNGRWPVVEKLFSAIAKHEASITGFLVILALSLGAYTGFLLSALTTYPMLNNPVLPLLFL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
VSGLSSGAAGTLLGGVLLKGNPDGKEVHFIHNIEIPMILVEIVLIFTFFTGLILTGGQSQ
HHCCCCCCHHHHHHCEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHH
VAAFAAIGEGFWGWIFWAGIVVVGLSMPLAFNLFMKASSKRKFSYVAITASCSLIGVLLL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHH
RNFILYTGQMTVA
HHHHHHCCCEECC
>Mature Secondary Structure 
SPFHFDSLTWPWPIAIYLFLAGISAGAICFAVLLKHFKLGRDAYKSGFVQAACLIAPLA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFAGLGILVIDLTKPFDFWKILVFYNPTSVMSVGVAVLMVYQVALFAWLACVFKEPALSW
HHHCCCEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
CNGRWPVVEKLFSAIAKHEASITGFLVILALSLGAYTGFLLSALTTYPMLNNPVLPLLFL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
VSGLSSGAAGTLLGGVLLKGNPDGKEVHFIHNIEIPMILVEIVLIFTFFTGLILTGGQSQ
HHCCCCCCHHHHHHCEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHH
VAAFAAIGEGFWGWIFWAGIVVVGLSMPLAFNLFMKASSKRKFSYVAITASCSLIGVLLL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHH
RNFILYTGQMTVA
HHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]