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The map label for this gene is nrfC [H]

Identifier: 157963777

GI number: 157963777

Start: 4820273

End: 4820956

Strand: Reverse

Name: nrfC [H]

Synonym: Spea_3966

Alternate gene names: 157963777

Gene position: 4820956-4820273 (Counterclockwise)

Preceding gene: 157963778

Following gene: 157963776

Centisome position: 93.17

GC content: 45.76

Gene sequence:

>684_bases
ATGTCTAACTCAAGAAGATTGTTTTTAAAAGGTGCCTGTGCAGCGGCGGTTGGGGCTTCCGGCTTCACCATCGCAACGGC
CAAAGGGACTGCTGGTAGCGACGTTGAACCCGTATGTAACTATGCCTTAGTACATGATGAGACCAAGTGTATCGGTTGTG
ATGCTTGCTCTATTGCTTGTCGTGAAGTCAACCAAGTACCAGAAGGTGTAAGCCGATTAGAGATCGAGCGTGAAGGCCCA
TTTGGTGAGTATCCAAATCAGCATTTTCGATTTACGCGTAAATCATGTCAGCATTGTGATGATACGCCTTGTGTAAAGGT
ATGCCCAACAGGGGCTGCCTATAAAGACCCAAAAACAGGCATCGTAAGTGTCGATGAATGGAAGTGTGTCGGTTGCCAAT
ACTGCATTGCTGCTTGTCCGTATCAAATCCGTTTCATTAATCCAGTGACTAAAGCGGCTGATAAATGCGATTTCTGTAAG
GAAACTCGCCTTAAAGAGGGTCAGTTACCTGCTTGTGTTACTGCCTGTCCAACTAAAGCGTTAACTTTTGGTGATATTAC
TGACCCGCAATCTCAAGTCGTACAAGTGCTTAAATCAGCCCCTAGCTACCGAACTAAAACTGATCTCGGGACCAGGCCTA
AGTTGTTCCGTATTGCATCACAAAGTGGGGAGATAAAACTATGA

Upstream 100 bases:

>100_bases
TTTTACATCCCAACACAGATCCTGTGCTAGGCGTATCTGCAAAAGAGCGTAGTGAACTGTGTAGCAGTTGCCACGTGGCC
AAGTAATTAGGGGAATCTAT

Downstream 100 bases:

>100_bases
GTCCATTCCATTTTGACAGCCTAACTTGGCCATGGCCAATTGCCATCTACCTATTTCTTGCGGGTATATCGGCCGGAGCC
ATCTGCTTTGCTGTATTGCT

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSNSRRLFLKGACAAAVGASGFTIATAKGTAGSDVEPVCNYALVHDETKCIGCDACSIACREVNQVPEGVSRLEIEREGP
FGEYPNQHFRFTRKSCQHCDDTPCVKVCPTGAAYKDPKTGIVSVDEWKCVGCQYCIAACPYQIRFINPVTKAADKCDFCK
ETRLKEGQLPACVTACPTKALTFGDITDPQSQVVQVLKSAPSYRTKTDLGTRPKLFRIASQSGEIKL

Sequences:

>Translated_227_residues
MSNSRRLFLKGACAAAVGASGFTIATAKGTAGSDVEPVCNYALVHDETKCIGCDACSIACREVNQVPEGVSRLEIEREGP
FGEYPNQHFRFTRKSCQHCDDTPCVKVCPTGAAYKDPKTGIVSVDEWKCVGCQYCIAACPYQIRFINPVTKAADKCDFCK
ETRLKEGQLPACVTACPTKALTFGDITDPQSQVVQVLKSAPSYRTKTDLGTRPKLFRIASQSGEIKL
>Mature_226_residues
SNSRRLFLKGACAAAVGASGFTIATAKGTAGSDVEPVCNYALVHDETKCIGCDACSIACREVNQVPEGVSRLEIEREGPF
GEYPNQHFRFTRKSCQHCDDTPCVKVCPTGAAYKDPKTGIVSVDEWKCVGCQYCIAACPYQIRFINPVTKAADKCDFCKE
TRLKEGQLPACVTACPTKALTFGDITDPQSQVVQVLKSAPSYRTKTDLGTRPKLFRIASQSGEIKL

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=230, Percent_Identity=58.2608695652174, Blast_Score=271, Evalue=3e-74,
Organism=Escherichia coli, GI226510944, Length=222, Percent_Identity=44.1441441441441, Blast_Score=187, Evalue=6e-49,
Organism=Escherichia coli, GI1789370, Length=225, Percent_Identity=31.5555555555556, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787872, Length=184, Percent_Identity=35.3260869565217, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787122, Length=184, Percent_Identity=34.7826086956522, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790326, Length=154, Percent_Identity=35.0649350649351, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1787749, Length=172, Percent_Identity=33.7209302325581, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787963, Length=207, Percent_Identity=30.4347826086957, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789067, Length=161, Percent_Identity=34.1614906832298, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI2367245, Length=155, Percent_Identity=33.5483870967742, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1787740, Length=104, Percent_Identity=34.6153846153846, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082180, Length=193, Percent_Identity=29.5336787564767, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789079, Length=226, Percent_Identity=29.2035398230088, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787478, Length=104, Percent_Identity=33.6538461538462, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788811, Length=175, Percent_Identity=30.2857142857143, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082179, Length=152, Percent_Identity=30.2631578947368, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24604; Mature: 24473

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
8.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSRRLFLKGACAAAVGASGFTIATAKGTAGSDVEPVCNYALVHDETKCIGCDACSIAC
CCCCCEEEEECCHHHHHCCCCEEEEEECCCCCCCCHHHHCEEEEECCCCEECCCHHHHHH
REVNQVPEGVSRLEIEREGPFGEYPNQHFRFTRKSCQHCDDTPCVKVCPTGAAYKDPKTG
HHHHHHHCCHHHEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCC
IVSVDEWKCVGCQYCIAACPYQIRFINPVTKAADKCDFCKETRLKEGQLPACVTACPTKA
EEECCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
LTFGDITDPQSQVVQVLKSAPSYRTKTDLGTRPKLFRIASQSGEIKL
EECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
SNSRRLFLKGACAAAVGASGFTIATAKGTAGSDVEPVCNYALVHDETKCIGCDACSIAC
CCCCEEEEECCHHHHHCCCCEEEEEECCCCCCCCHHHHCEEEEECCCCEECCCHHHHHH
REVNQVPEGVSRLEIEREGPFGEYPNQHFRFTRKSCQHCDDTPCVKVCPTGAAYKDPKTG
HHHHHHHCCHHHEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCC
IVSVDEWKCVGCQYCIAACPYQIRFINPVTKAADKCDFCKETRLKEGQLPACVTACPTKA
EEECCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
LTFGDITDPQSQVVQVLKSAPSYRTKTDLGTRPKLFRIASQSGEIKL
EECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]