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The map label for this gene is gcvA [H]

Identifier: 157963665

GI number: 157963665

Start: 4684001

End: 4684903

Strand: Reverse

Name: gcvA [H]

Synonym: Spea_3854

Alternate gene names: 157963665

Gene position: 4684903-4684001 (Counterclockwise)

Preceding gene: 157963666

Following gene: 157963664

Centisome position: 90.54

GC content: 45.96

Gene sequence:

>903_bases
ATGAATACAACTCAAGTTAGTTCAAGTGCCCGCTTACCGCAGTTATCTTGGTTTATGGCATTTAAAGCGGTGGCAGATAA
ACAAAGCTTTACCGAGGCCAGCCGTGAGCTTTGTCTTACGCAGTCAGCCATTAGCCAACAAGTGGCCAAGCTTGAAGCCG
TGCTACGTACCAAGTTGTTTTATCGTGGCGGACGACACATCCAACTAACAGAAGATGGACAGCGATTACTGTTACAGGTT
AATCAGCCCATCCAAGAATTAATGGGCGTGGTGGATGGCTTTCATAATAATGCCGAGCATTACACCTTACATATTGAGAT
GGAGCCGGTTTTTAGCCGCCAGGTTGTCAGTAAATTATTACCGAAATTTTTAGCCCAGCACCCTAACTTATTGGTCGGGC
AGATGCTGACCACCAATCACTTTGATTTTTTACCGCAAACTGAGTTAGCGATAAAATGGGGTGACGGTGAATGGGAAGGC
TTTGATTCGCAGTTTTTGTGCAGCTTGGATTATGTGCCTGTGTGCTCGCCAGAGTACCTCAAGCGCAAACCTCTGACTGT
ACCGGCAGACTTGGCTAAGGCCAACATTATCCATGACCGAGACAATTTCGATTGGAAGTATTGGCTTAATTATTATCCGG
TGGCTCGCTTAGAGCTACAAAAGTGCCATTATGCCAGTGAGAGCCAAGTGGTGATGTCGCTAGCGATGAGTGGTTTAGGG
GTTGCTGTTTGTGCCTATCAGCAGATCCAAGAAGAGTTAGCCGATGGTCGTTTGGTGATGCCGTTTCCTGAGTTAAAGGT
GCGTCATCAGCGCGCTTATTATATTTTAACCCGTAAGAATAAGTCGTTATCGCCACAAGCCCATAGTTTTATGCAGTTTG
TCCGAGAAGCTATGTTGACGTAA

Upstream 100 bases:

>100_bases
CTGTTAAGCTTTTTCAACGTCTAGCCGCTAAGCGCTAGTAGCTAGTTAAGCATCGCCCTTATACTTATATAGGTTTAAGG
GCTTACCATCGCAGGATGTT

Downstream 100 bases:

>100_bases
ATCCTGCATGGTTTAACTTTATTTAGCTTGTTCAATCACTTACATTATACGCAATCACTATTTGATCCATCAGTAAACGA
TATGAAAATCACCAAAGAAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MNTTQVSSSARLPQLSWFMAFKAVADKQSFTEASRELCLTQSAISQQVAKLEAVLRTKLFYRGGRHIQLTEDGQRLLLQV
NQPIQELMGVVDGFHNNAEHYTLHIEMEPVFSRQVVSKLLPKFLAQHPNLLVGQMLTTNHFDFLPQTELAIKWGDGEWEG
FDSQFLCSLDYVPVCSPEYLKRKPLTVPADLAKANIIHDRDNFDWKYWLNYYPVARLELQKCHYASESQVVMSLAMSGLG
VAVCAYQQIQEELADGRLVMPFPELKVRHQRAYYILTRKNKSLSPQAHSFMQFVREAMLT

Sequences:

>Translated_300_residues
MNTTQVSSSARLPQLSWFMAFKAVADKQSFTEASRELCLTQSAISQQVAKLEAVLRTKLFYRGGRHIQLTEDGQRLLLQV
NQPIQELMGVVDGFHNNAEHYTLHIEMEPVFSRQVVSKLLPKFLAQHPNLLVGQMLTTNHFDFLPQTELAIKWGDGEWEG
FDSQFLCSLDYVPVCSPEYLKRKPLTVPADLAKANIIHDRDNFDWKYWLNYYPVARLELQKCHYASESQVVMSLAMSGLG
VAVCAYQQIQEELADGRLVMPFPELKVRHQRAYYILTRKNKSLSPQAHSFMQFVREAMLT
>Mature_300_residues
MNTTQVSSSARLPQLSWFMAFKAVADKQSFTEASRELCLTQSAISQQVAKLEAVLRTKLFYRGGRHIQLTEDGQRLLLQV
NQPIQELMGVVDGFHNNAEHYTLHIEMEPVFSRQVVSKLLPKFLAQHPNLLVGQMLTTNHFDFLPQTELAIKWGDGEWEG
FDSQFLCSLDYVPVCSPEYLKRKPLTVPADLAKANIIHDRDNFDWKYWLNYYPVARLELQKCHYASESQVVMSLAMSGLG
VAVCAYQQIQEELADGRLVMPFPELKVRHQRAYYILTRKNKSLSPQAHSFMQFVREAMLT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=31.25, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=28.0405405405405, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786448, Length=250, Percent_Identity=28, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=23.7931034482759, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789639, Length=277, Percent_Identity=25.2707581227437, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI157672245, Length=169, Percent_Identity=31.3609467455621, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87081978, Length=263, Percent_Identity=23.574144486692, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34459; Mature: 34459

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTQVSSSARLPQLSWFMAFKAVADKQSFTEASRELCLTQSAISQQVAKLEAVLRTKLF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRGGRHIQLTEDGQRLLLQVNQPIQELMGVVDGFHNNAEHYTLHIEMEPVFSRQVVSKLL
HCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHH
PKFLAQHPNLLVGQMLTTNHFDFLPQTELAIKWGDGEWEGFDSQFLCSLDYVPVCSPEYL
HHHHHCCCCEEEHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCHHHH
KRKPLTVPADLAKANIIHDRDNFDWKYWLNYYPVARLELQKCHYASESQVVMSLAMSGLG
CCCCCCCCHHHHHHHEEECCCCCCEEEEECCCCCHHEEHHHHCCCCHHHHHHHHHHHCCH
VAVCAYQQIQEELADGRLVMPFPELKVRHQRAYYILTRKNKSLSPQAHSFMQFVREAMLT
HHHHHHHHHHHHHHCCEEEECCHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTTQVSSSARLPQLSWFMAFKAVADKQSFTEASRELCLTQSAISQQVAKLEAVLRTKLF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRGGRHIQLTEDGQRLLLQVNQPIQELMGVVDGFHNNAEHYTLHIEMEPVFSRQVVSKLL
HCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHH
PKFLAQHPNLLVGQMLTTNHFDFLPQTELAIKWGDGEWEGFDSQFLCSLDYVPVCSPEYL
HHHHHCCCCEEEHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCHHHH
KRKPLTVPADLAKANIIHDRDNFDWKYWLNYYPVARLELQKCHYASESQVVMSLAMSGLG
CCCCCCCCHHHHHHHEEECCCCCCEEEEECCCCCHHEEHHHHCCCCHHHHHHHHHHHCCH
VAVCAYQQIQEELADGRLVMPFPELKVRHQRAYYILTRKNKSLSPQAHSFMQFVREAMLT
HHHHHHHHHHHHHHCCEEEECCHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]