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The map label for this gene is glpK [H]

Identifier: 157963633

GI number: 157963633

Start: 4645886

End: 4647367

Strand: Reverse

Name: glpK [H]

Synonym: Spea_3822

Alternate gene names: 157963633

Gene position: 4647367-4645886 (Counterclockwise)

Preceding gene: 157963634

Following gene: 157963631

Centisome position: 89.81

GC content: 49.06

Gene sequence:

>1482_bases
GTGTCAAAGAAATACATCATAGCACTGGACCAAGGGACAACGAGCTCCCGAGCGATAGTGTTCGATCATGATACCAAGAT
GGTTGCTAGCTCACAGCGTGAGTTTAGCCAAATGTATCCTCAACCGGGTTGGGTTGAACATGATGCGATGGAGATCTGGG
CGTCTCAAAGCTCAACTCTTATTGAAGTATTAGCGCGGGCGGATATTCACAGTGAAGATGTGGCGGCTATCGGCATTACT
AATCAGAGAGAAACGACAGTCGTTTGGGACAAGGTAACGGGTAAGCCTGTTTACAACGCAATCGTATGGCAGTGCCGACG
CAGTAAAGCCATTTGTGACGAGCTCAAAGCGCAAGGGTTAGAAGATTACATTAAGCAGACTACAGGCTTGGTACTCGATC
CTTATTTTTCAGGCACCAAGATTAAGTGGATTTTGGATAACGTCGAAGGTGTGCGGGAGCGAGCAGAGAAAGGTGAGCTA
CTGTTTGGCACCATAGATACCTGGCTTGTTTGGAAGCTCACTGAGGGCAAGGTTCATGTTACCGATCCGACCAATGCTTC
ACGGACCATGTTATTCAATATTCATACTCAGCAGTGGGACGACAAGCTACTAGATGCATTTGATATTCCGCGCTCAATAC
TGCCAGAGGTAAAGCCTTCGTCGGCTATCTATGGTTATACCCGTATAGCGGGGGAAGGTAGTCACATTGCCATTGCTGGG
ATGGCGGGAGATCAGCAGTCGGCCTTGTTTGGTCAGCTCTGCATCGAAGAAGGCATGGCAAAAAACACTTATGGTACAGG
CTGTTTCTTGCTGATGAATACTGGTGTCGAAGCCGTTCAGTCTCAGCACGGATTGTTAACCACCATTGCGATTGGAGCCG
ACGGTGGAATTAACTATGCATTAGAGGGCTCGGTATTTATGGGCGGAGCTACAGTGCAATGGCTTAGGGATGAGTTGGGG
CTTATTCGTGATGCACAAGATACCGAATACTTTGCTAAAAAAGTTGAAGATACTAATGGTGTGTATCTGATCCCGGCATT
CGTTGGATTAGGCGCTCCCTATTGGGACCCTGACGCGAGAGGAGCGTTAGTGGGCTTAACCCGAGGGGCTAATCGTAACC
ATATTATTCGTGCTGCACTGGAGGCTATTGCATATCAGAGCCGCGACCTATTGGATGCCATGTCTAAAGATAGTTGTGTC
GAGCTGAAGCAGATTAAGGTCGACGGCGGCGCGGTGGCGAATGACTTCCTGATGCAGTTTCAGGCCGATATTACTAACGT
TGAGGTTTTGCGCCCAGAGCTGACTGAAACAACTGCACTGGGAGCGGCATTTCTGGCAGGTCTAGCGGTGGGCTTCTGGG
ACTCTACCGATGAACTAAAGCATAAGGCTGGCATCGAGCGTAACTTTACACCACAGATCAGCATGCAAAAGCGCGATAGT
CTATACAAAGGTTGGCAAGAGGCTGTGACCCGGACACGTTAA

Upstream 100 bases:

>100_bases
CGCCTGTGATGTGACGCCTTGGGTTGAAGTGGTAAGCCCTGAAATTGTTCAACCAGAAATTGGTTTATTAAAAGAAACGC
GCCAATAAGGAAAGCAAAAA

Downstream 100 bases:

>100_bases
GGGCGAGTGATACCTGCGGTCAAACATCGATGTCGATCCGAGTGTGGATCGACTCTGGAATATCTTCACTTTTTTGTTCT
AGTGATGACGCTTGCTCCTT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSKKYIIALDQGTTSSRAIVFDHDTKMVASSQREFSQMYPQPGWVEHDAMEIWASQSSTLIEVLARADIHSEDVAAIGIT
NQRETTVVWDKVTGKPVYNAIVWQCRRSKAICDELKAQGLEDYIKQTTGLVLDPYFSGTKIKWILDNVEGVRERAEKGEL
LFGTIDTWLVWKLTEGKVHVTDPTNASRTMLFNIHTQQWDDKLLDAFDIPRSILPEVKPSSAIYGYTRIAGEGSHIAIAG
MAGDQQSALFGQLCIEEGMAKNTYGTGCFLLMNTGVEAVQSQHGLLTTIAIGADGGINYALEGSVFMGGATVQWLRDELG
LIRDAQDTEYFAKKVEDTNGVYLIPAFVGLGAPYWDPDARGALVGLTRGANRNHIIRAALEAIAYQSRDLLDAMSKDSCV
ELKQIKVDGGAVANDFLMQFQADITNVEVLRPELTETTALGAAFLAGLAVGFWDSTDELKHKAGIERNFTPQISMQKRDS
LYKGWQEAVTRTR

Sequences:

>Translated_493_residues
MSKKYIIALDQGTTSSRAIVFDHDTKMVASSQREFSQMYPQPGWVEHDAMEIWASQSSTLIEVLARADIHSEDVAAIGIT
NQRETTVVWDKVTGKPVYNAIVWQCRRSKAICDELKAQGLEDYIKQTTGLVLDPYFSGTKIKWILDNVEGVRERAEKGEL
LFGTIDTWLVWKLTEGKVHVTDPTNASRTMLFNIHTQQWDDKLLDAFDIPRSILPEVKPSSAIYGYTRIAGEGSHIAIAG
MAGDQQSALFGQLCIEEGMAKNTYGTGCFLLMNTGVEAVQSQHGLLTTIAIGADGGINYALEGSVFMGGATVQWLRDELG
LIRDAQDTEYFAKKVEDTNGVYLIPAFVGLGAPYWDPDARGALVGLTRGANRNHIIRAALEAIAYQSRDLLDAMSKDSCV
ELKQIKVDGGAVANDFLMQFQADITNVEVLRPELTETTALGAAFLAGLAVGFWDSTDELKHKAGIERNFTPQISMQKRDS
LYKGWQEAVTRTR
>Mature_492_residues
SKKYIIALDQGTTSSRAIVFDHDTKMVASSQREFSQMYPQPGWVEHDAMEIWASQSSTLIEVLARADIHSEDVAAIGITN
QRETTVVWDKVTGKPVYNAIVWQCRRSKAICDELKAQGLEDYIKQTTGLVLDPYFSGTKIKWILDNVEGVRERAEKGELL
FGTIDTWLVWKLTEGKVHVTDPTNASRTMLFNIHTQQWDDKLLDAFDIPRSILPEVKPSSAIYGYTRIAGEGSHIAIAGM
AGDQQSALFGQLCIEEGMAKNTYGTGCFLLMNTGVEAVQSQHGLLTTIAIGADGGINYALEGSVFMGGATVQWLRDELGL
IRDAQDTEYFAKKVEDTNGVYLIPAFVGLGAPYWDPDARGALVGLTRGANRNHIIRAALEAIAYQSRDLLDAMSKDSCVE
LKQIKVDGGAVANDFLMQFQADITNVEVLRPELTETTALGAAFLAGLAVGFWDSTDELKHKAGIERNFTPQISMQKRDSL
YKGWQEAVTRTR

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=509, Percent_Identity=46.7583497053045, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI189409120, Length=509, Percent_Identity=46.7583497053045, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI42794763, Length=514, Percent_Identity=46.4980544747082, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI41393575, Length=498, Percent_Identity=46.5863453815261, Blast_Score=447, Evalue=1e-126,
Organism=Homo sapiens, GI88196792, Length=504, Percent_Identity=34.1269841269841, Blast_Score=302, Evalue=6e-82,
Organism=Escherichia coli, GI1790361, Length=490, Percent_Identity=70, Blast_Score=749, Evalue=0.0,
Organism=Escherichia coli, GI1787789, Length=519, Percent_Identity=25.626204238921, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789987, Length=508, Percent_Identity=24.8031496062992, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789138, Length=506, Percent_Identity=23.1225296442688, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790005, Length=487, Percent_Identity=21.9712525667351, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17535599, Length=498, Percent_Identity=42.570281124498, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71986140, Length=519, Percent_Identity=24.0847784200385, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI71986147, Length=262, Percent_Identity=29.3893129770992, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321755, Length=545, Percent_Identity=36.697247706422, Blast_Score=300, Evalue=3e-82,
Organism=Drosophila melanogaster, GI17864214, Length=507, Percent_Identity=44.1814595660749, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24654573, Length=432, Percent_Identity=46.9907407407407, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655555, Length=497, Percent_Identity=40.0402414486922, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655560, Length=497, Percent_Identity=40.0402414486922, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655570, Length=497, Percent_Identity=40.0402414486922, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655574, Length=497, Percent_Identity=40.0402414486922, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655565, Length=497, Percent_Identity=40.0402414486922, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI19922058, Length=521, Percent_Identity=34.3570057581574, Blast_Score=298, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24652871, Length=488, Percent_Identity=35.2459016393443, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24656367, Length=493, Percent_Identity=29.2089249492901, Blast_Score=218, Evalue=1e-56,
Organism=Drosophila melanogaster, GI21357867, Length=514, Percent_Identity=28.0155642023346, Blast_Score=211, Evalue=9e-55,
Organism=Drosophila melanogaster, GI24656372, Length=341, Percent_Identity=31.0850439882698, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45552909, Length=306, Percent_Identity=27.4509803921569, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54418; Mature: 54287

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKYIIALDQGTTSSRAIVFDHDTKMVASSQREFSQMYPQPGWVEHDAMEIWASQSSTL
CCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
IEVLARADIHSEDVAAIGITNQRETTVVWDKVTGKPVYNAIVWQCRRSKAICDELKAQGL
HHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
EDYIKQTTGLVLDPYFSGTKIKWILDNVEGVRERAEKGELLFGTIDTWLVWKLTEGKVHV
HHHHHHHCCEEEECCCCCCEEEEEEHHHHHHHHHHHCCCEEEEEECEEEEEEEECCEEEE
TDPTNASRTMLFNIHTQQWDDKLLDAFDIPRSILPEVKPSSAIYGYTRIAGEGSHIAIAG
ECCCCCCEEEEEEEECCCHHHHHHHHHCCCHHHCCCCCCCCEEEEEEEECCCCCEEEEEE
MAGDQQSALFGQLCIEEGMAKNTYGTGCFLLMNTGVEAVQSQHGLLTTIAIGADGGINYA
ECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEECCCCCCEE
LEGSVFMGGATVQWLRDELGLIRDAQDTEYFAKKVEDTNGVYLIPAFVGLGAPYWDPDAR
EECCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCC
GALVGLTRGANRNHIIRAALEAIAYQSRDLLDAMSKDSCVELKQIKVDGGAVANDFLMQF
CEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHH
QADITNVEVLRPELTETTALGAAFLAGLAVGFWDSTDELKHKAGIERNFTPQISMQKRDS
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHH
LYKGWQEAVTRTR
HHHHHHHHHHCCC
>Mature Secondary Structure 
SKKYIIALDQGTTSSRAIVFDHDTKMVASSQREFSQMYPQPGWVEHDAMEIWASQSSTL
CCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
IEVLARADIHSEDVAAIGITNQRETTVVWDKVTGKPVYNAIVWQCRRSKAICDELKAQGL
HHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
EDYIKQTTGLVLDPYFSGTKIKWILDNVEGVRERAEKGELLFGTIDTWLVWKLTEGKVHV
HHHHHHHCCEEEECCCCCCEEEEEEHHHHHHHHHHHCCCEEEEEECEEEEEEEECCEEEE
TDPTNASRTMLFNIHTQQWDDKLLDAFDIPRSILPEVKPSSAIYGYTRIAGEGSHIAIAG
ECCCCCCEEEEEEEECCCHHHHHHHHHCCCHHHCCCCCCCCEEEEEEEECCCCCEEEEEE
MAGDQQSALFGQLCIEEGMAKNTYGTGCFLLMNTGVEAVQSQHGLLTTIAIGADGGINYA
ECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEECCCCCCEE
LEGSVFMGGATVQWLRDELGLIRDAQDTEYFAKKVEDTNGVYLIPAFVGLGAPYWDPDAR
EECCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCC
GALVGLTRGANRNHIIRAALEAIAYQSRDLLDAMSKDSCVELKQIKVDGGAVANDFLMQF
CEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHH
QADITNVEVLRPELTETTALGAAFLAGLAVGFWDSTDELKHKAGIERNFTPQISMQKRDS
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHH
LYKGWQEAVTRTR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA