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The map label for this gene is murD [H]

Identifier: 157963624

GI number: 157963624

Start: 4636211

End: 4637530

Strand: Reverse

Name: murD [H]

Synonym: Spea_3813

Alternate gene names: 157963624

Gene position: 4637530-4636211 (Counterclockwise)

Preceding gene: 157963625

Following gene: 157963623

Centisome position: 89.62

GC content: 49.17

Gene sequence:

>1320_bases
ATGGCGCAGCAATATTCACACATAGTTTTAGGTTTAGGTGCGACAGGTTTGTCTGTTGTACGTTACCTGTGTGCGCAAGG
GATAGTTCCTTTAGTCATGGACAGCCGAGCTCAGCCTCCGGGAGCTGATAGCTTGGCGACAGAGTTTCCTCAAGTTGAGC
TAGTCACTGGTGGTTTTGATTGTCGCTATCTAGTACAGGCACAGCAGATTGTCATTAGTCCTGGTATCGCCTGCGAAACG
CCAGAAGTGCGAGTCGCATTTGATATGGGGATTGAAGTGATTGGCGATGTTGAGCTATTTGCACGGGCAGTCGCGCAAAC
CTCAGCCAAGGTCATAGGTATTACTGGCTCAAATGGTAAGACAACTGTCACGACCTTAGTCGGTGAGATGGCTAAGGCTA
GCGGCAGCTCTTACGCCGTGGGTGGCAACATTGGTGTGCCAGCTCTTGACCTATTGCTCGAACCTAAAGATCTGTATGTC
CTCGAGTTGTCGAGTTTTCAGTTAGAGACAACCTATAGCTTGCAGTGTGTTGCGGCGACTTGTCTTAACATTAGCGAAGA
TCATATGGATCGCTATAGCGATCTTGAGTCTTATCGTCAGGCGAAGCTTAAGTTATACGAGCAGAGCAAGATGGCGGTGT
TTAATCGTGAAGACACGTTAACGCTACCGAACAACCCGACTAACCAACAAAGCTTTGGTTTAGATGCGCCAGAGGGCGAT
GATTGGGGCGTTTCGGGCGGCATGATAGTGCAAGGTAGTAGCCAGATCATGCCGATTAGCGATGTCGCACTCGTAGGGAG
CCACAACCTTGCCAATATTATTGCAGCGATGGCATTGACCACTGCGGCGGGGATTAAACGTGAGTGTCAGATTGAGGTGG
CTAAGACGTTTTTAGGCCTTGCTCATCGCTGTGAAGTGGTTGCAGATATTGATGGCGTCACTTATGTCAACGACTCGAAG
GCCACGAATATCGGCGCTACAGTCGCTGCTATTGATGGTTTGAGTGAGCACTTAGGTGAATTGATCTTGATCGCAGGTGG
CGATGGCAAAGGGGCTGACTTTAAACCGTTAGAGCCCGTGCTTGAGAAGGTGAGTCAGCTAATCGTTTTAGGTAAAGATG
GCGACAAAATCGCAGGGTTGAAACCGGAGGCGATAAAAGTCGCCAATATGGTTGAAGCGGTAGCAAAAGCGCATGAGCTA
GCTACAGCAGGTGATATTGTTATTCTGTCACCGGCTTGTTCAAGCTTAGATATGTACCCCAACTTTATGGCTCGTGGAGA
TGAATTCCGTAGCCTGGTTGAAGCGCTTCATGGCGAGTAG

Upstream 100 bases:

>100_bases
TCTCTTTGTTCTTAGTCTTGCTTGGATTAGCAACCTTGAAACTGAGATAACAGATTTAAATTAACGAACTCATTTAACTT
AGATACTAGATAGGTCTGAT

Downstream 100 bases:

>100_bases
CGAGAAGCAGTTAAGTTTATTTGGCAACCGCTTAAAATGGTCGTTGCCGAATCTGTTTAGCGACAACTCAGCCCCTGGGA
TGCAGCTGTACGATAGAGCA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MAQQYSHIVLGLGATGLSVVRYLCAQGIVPLVMDSRAQPPGADSLATEFPQVELVTGGFDCRYLVQAQQIVISPGIACET
PEVRVAFDMGIEVIGDVELFARAVAQTSAKVIGITGSNGKTTVTTLVGEMAKASGSSYAVGGNIGVPALDLLLEPKDLYV
LELSSFQLETTYSLQCVAATCLNISEDHMDRYSDLESYRQAKLKLYEQSKMAVFNREDTLTLPNNPTNQQSFGLDAPEGD
DWGVSGGMIVQGSSQIMPISDVALVGSHNLANIIAAMALTTAAGIKRECQIEVAKTFLGLAHRCEVVADIDGVTYVNDSK
ATNIGATVAAIDGLSEHLGELILIAGGDGKGADFKPLEPVLEKVSQLIVLGKDGDKIAGLKPEAIKVANMVEAVAKAHEL
ATAGDIVILSPACSSLDMYPNFMARGDEFRSLVEALHGE

Sequences:

>Translated_439_residues
MAQQYSHIVLGLGATGLSVVRYLCAQGIVPLVMDSRAQPPGADSLATEFPQVELVTGGFDCRYLVQAQQIVISPGIACET
PEVRVAFDMGIEVIGDVELFARAVAQTSAKVIGITGSNGKTTVTTLVGEMAKASGSSYAVGGNIGVPALDLLLEPKDLYV
LELSSFQLETTYSLQCVAATCLNISEDHMDRYSDLESYRQAKLKLYEQSKMAVFNREDTLTLPNNPTNQQSFGLDAPEGD
DWGVSGGMIVQGSSQIMPISDVALVGSHNLANIIAAMALTTAAGIKRECQIEVAKTFLGLAHRCEVVADIDGVTYVNDSK
ATNIGATVAAIDGLSEHLGELILIAGGDGKGADFKPLEPVLEKVSQLIVLGKDGDKIAGLKPEAIKVANMVEAVAKAHEL
ATAGDIVILSPACSSLDMYPNFMARGDEFRSLVEALHGE
>Mature_438_residues
AQQYSHIVLGLGATGLSVVRYLCAQGIVPLVMDSRAQPPGADSLATEFPQVELVTGGFDCRYLVQAQQIVISPGIACETP
EVRVAFDMGIEVIGDVELFARAVAQTSAKVIGITGSNGKTTVTTLVGEMAKASGSSYAVGGNIGVPALDLLLEPKDLYVL
ELSSFQLETTYSLQCVAATCLNISEDHMDRYSDLESYRQAKLKLYEQSKMAVFNREDTLTLPNNPTNQQSFGLDAPEGDD
WGVSGGMIVQGSSQIMPISDVALVGSHNLANIIAAMALTTAAGIKRECQIEVAKTFLGLAHRCEVVADIDGVTYVNDSKA
TNIGATVAAIDGLSEHLGELILIAGGDGKGADFKPLEPVLEKVSQLIVLGKDGDKIAGLKPEAIKVANMVEAVAKAHELA
TAGDIVILSPACSSLDMYPNFMARGDEFRSLVEALHGE

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=446, Percent_Identity=48.2062780269058, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 46321; Mature: 46190

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQQYSHIVLGLGATGLSVVRYLCAQGIVPLVMDSRAQPPGADSLATEFPQVELVTGGFD
CCCCCCEEEEECCCHHHHHHHHHHHCCCEEEHCCCCCCCCCCCHHHHCCCCEEEEECCCC
CRYLVQAQQIVISPGIACETPEVRVAFDMGIEVIGDVELFARAVAQTSAKVIGITGSNGK
EEEEEEEEEEEECCCCCCCCCCEEEEEECCCEEECCHHHHHHHHHHCCCEEEEEECCCCC
TTVTTLVGEMAKASGSSYAVGGNIGVPALDLLLEPKDLYVLELSSFQLETTYSLQCVAAT
EEHHHHHHHHHHCCCCCEEECCCCCCCHHHEEECCCCEEEEEECCEEEEEEEEHHHHHHH
CLNISEDHMDRYSDLESYRQAKLKLYEQSKMAVFNREDTLTLPNNPTNQQSFGLDAPEGD
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEECCCCCCCCCCCCCCCCCCC
DWGVSGGMIVQGSSQIMPISDVALVGSHNLANIIAAMALTTAAGIKRECQIEVAKTFLGL
CCCCCCCEEEECCCCCCCHHHEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AHRCEVVADIDGVTYVNDSKATNIGATVAAIDGLSEHLGELILIAGGDGKGADFKPLEPV
HHHCEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHH
LEKVSQLIVLGKDGDKIAGLKPEAIKVANMVEAVAKAHELATAGDIVILSPACSSLDMYP
HHHHHHEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
NFMARGDEFRSLVEALHGE
HHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AQQYSHIVLGLGATGLSVVRYLCAQGIVPLVMDSRAQPPGADSLATEFPQVELVTGGFD
CCCCCEEEEECCCHHHHHHHHHHHCCCEEEHCCCCCCCCCCCHHHHCCCCEEEEECCCC
CRYLVQAQQIVISPGIACETPEVRVAFDMGIEVIGDVELFARAVAQTSAKVIGITGSNGK
EEEEEEEEEEEECCCCCCCCCCEEEEEECCCEEECCHHHHHHHHHHCCCEEEEEECCCCC
TTVTTLVGEMAKASGSSYAVGGNIGVPALDLLLEPKDLYVLELSSFQLETTYSLQCVAAT
EEHHHHHHHHHHCCCCCEEECCCCCCCHHHEEECCCCEEEEEECCEEEEEEEEHHHHHHH
CLNISEDHMDRYSDLESYRQAKLKLYEQSKMAVFNREDTLTLPNNPTNQQSFGLDAPEGD
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEECCCCCCCCCCCCCCCCCCC
DWGVSGGMIVQGSSQIMPISDVALVGSHNLANIIAAMALTTAAGIKRECQIEVAKTFLGL
CCCCCCCEEEECCCCCCCHHHEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AHRCEVVADIDGVTYVNDSKATNIGATVAAIDGLSEHLGELILIAGGDGKGADFKPLEPV
HHHCEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHH
LEKVSQLIVLGKDGDKIAGLKPEAIKVANMVEAVAKAHELATAGDIVILSPACSSLDMYP
HHHHHHEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
NFMARGDEFRSLVEALHGE
HHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA