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The map label for this gene is priA [H]

Identifier: 157963590

GI number: 157963590

Start: 4592443

End: 4594638

Strand: Direct

Name: priA [H]

Synonym: Spea_3778

Alternate gene names: 157963590

Gene position: 4592443-4594638 (Clockwise)

Preceding gene: 157963554

Following gene: 157963591

Centisome position: 88.75

GC content: 50.32

Gene sequence:

>2196_bases
ATGCCTTTGTTTGTTGAGGTCGCACTTCCTGTTCCAATGCGACAAAACTTTAGCTATCAAGTGCCTGAACATATCACCAT
TTCGCCTCAAAAGGGCATGCGTGTACAAGTGCCATTTGGTCGCCAACAACTTATCGGCCTGATTACGGCGACTAGTGATA
GCTGCTCGCTGACGCCTAAGCAGATCAAGCCTATTGTTGCCATTCTTGATACCGAGCCGCTGTTACCTGATTCACTTTAT
AAATTAACCGCTTGGGCTGCAAGGTATTACTTTTGTAGCCTTGGGCAAATGCTATCACAGGCCATTCCAGTAGCGCTGCG
TAAAGGCGCCGAAGTCAAAGCGTCGACCGTCAGCTATTGGACGCTAACGGAGGCGGGTAAAGAGGCTGATATCAATCAGC
TAAACCGAGCCCCCGCACAACGTAAGTTACTCCACTCTCTACGCGAACGAGATTTAGAACTTGGCGAGATCGCCAGCCTT
GAACTCAGCAAGGCGGCGGTAAAATCCTTAGATGAAAAAGGTTGGATAGAGAAGAAAAGCCGAACGCTTGATATAGACTT
AAGCTGGCGCAACGAACTGGAGATGACAGAGGAGCCACACAGGCTTAACCCTGAGCAAGCAATAGCCGTCGCCACCTTAA
CTCAACAGCAAGGCTACCACTGCACCCTGCTCGAAGGCGTCACAGGTTCCGGCAAGACCGAAGTCTATTTAGCCCTGCTC
GAAACCGTGCTAAAGCAAGGTAAGCAAGCTTTAGTGCTGGTGCCTGAAATTGGTCTTACACCACAAACCATTAACCGTTT
TAGACGCAGGTTTAATGTTAAGGTTGCGGTGATCCATTCAGGACTGACCGACAACCAAAGATTAGACGCTTGGCGTCAAG
CCAAATCTGGCGAAGCAGCCATCATCATTGGCACCCGCTCGGCGCTGTTTACCCCGATGCTTTACCCTGGGGTAATTATT
TTAGACGAAGAGCATGATTCGAGCTTTAAGCAACAAGAAGGCGTTGGCTATCATGCTAGAGACTTAGCCGTGATGCGTGG
TCACCTCGAACAGATCCCGGTACTTTTAGGAACGGCAACGCCTTCACTAGAAACATTACAAAATGCGCTCAATGGGCGCT
ACCAACATTTACATTTAGGTAAGCGTGCTGGTGCGGCCGAAAAGGTGCGTCAGGGGATCGTCGATATTCGCAATCAGCCA
CTGCGCTCTGGCATGTCGCACGCGCTATTAAATGAAATGCGTATTCACCTCGATGCGGGCAACCAGGTTATTCTGTTTTT
AAACCGCCGCGGCTTTGCCCCTGCGCTGATCTGTCATGAGTGTGGCCACCTACACGAGTGCGACCGCTGTGACGCCTTTT
TTACCGTGCATCAGGCCCTTGGCGAAATTCGCTGTCATCACTGCAGCAATCAATATGCGATTCCTAAACAATGCCACAGT
TGCGGCAGCACCATGCTGGCAGGCCAAGGTGTGGGCACCGAACAACTCGCATCATTTTTAGAAAAAGAGTTTCCTAATCA
TCCTGTGGTGCGTATCGACAGAGACACGACCCGTAATAAGGGCTCACTCGAAGGCCACCTTAACGCGATCCACAAAGGTG
AGTACAAAATCTTAGTCGGCACTCAGATGCTCGCCAAGGGGCATCACTTTCCTGATGTAACCTTAGTGGGCTTGCTCGAT
GTAGATGGTGCCCTGTTTAGCGCCGACTTTAGAGCACCTGAGCGCTTCGGTCAGCTCTACACTCAGGTATCTGGCCGTGC
TGGCCGCGCGACTAAACCCGGCACAGTGCTACTACAGACTCATCAAAGCGATAACGCCATGCTGCGTGAACTGATGCATA
AAGGTTATGGCGAATTTGCCCGCAACCAGCTGTTCGAGCGCACTCAAGCTCTGCTACCACCGGCATGGCACATGATTCTA
ATTCGCGCCGAAGCCCATCACGCTATCGACGCCGATAACTTACTAAACCAGATTGGAGCCTTACTGCCTCAAGATGAAGA
ATGCGAAGTGATTGGCCCCATGCCAGCGCCACTCGATAGGAAAGCGGGTAAATATCGTCGCCAATTATTATTTCAGACAA
AAAACCGTAATAGACTGCAGCTAGAATTCGAGCGTGCACTGCCACAAATCGAACAATTACCGCTGGCTAAAAAATGTCGT
TGGAGCATAGATAGAGACCCGCAAGATCTGCTTTAA

Upstream 100 bases:

>100_bases
AGATAAGTAAGCGCAATAACTAATAAAATAAATTGCCGCTAGCTCTGATTAAATAGGCTCGGTACACTTAAAGGCAGTAT
CCTTTCGAGCCTGTAACCCT

Downstream 100 bases:

>100_bases
ATCGACTTTATCTATATCAATGCAGAAAAAGTTCGCTAGAGGATAGTAATTCACCGCCACTAGCGGCTAAAATAGTCGGT
TACCCCTTATTATTTCTCTT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MPLFVEVALPVPMRQNFSYQVPEHITISPQKGMRVQVPFGRQQLIGLITATSDSCSLTPKQIKPIVAILDTEPLLPDSLY
KLTAWAARYYFCSLGQMLSQAIPVALRKGAEVKASTVSYWTLTEAGKEADINQLNRAPAQRKLLHSLRERDLELGEIASL
ELSKAAVKSLDEKGWIEKKSRTLDIDLSWRNELEMTEEPHRLNPEQAIAVATLTQQQGYHCTLLEGVTGSGKTEVYLALL
ETVLKQGKQALVLVPEIGLTPQTINRFRRRFNVKVAVIHSGLTDNQRLDAWRQAKSGEAAIIIGTRSALFTPMLYPGVII
LDEEHDSSFKQQEGVGYHARDLAVMRGHLEQIPVLLGTATPSLETLQNALNGRYQHLHLGKRAGAAEKVRQGIVDIRNQP
LRSGMSHALLNEMRIHLDAGNQVILFLNRRGFAPALICHECGHLHECDRCDAFFTVHQALGEIRCHHCSNQYAIPKQCHS
CGSTMLAGQGVGTEQLASFLEKEFPNHPVVRIDRDTTRNKGSLEGHLNAIHKGEYKILVGTQMLAKGHHFPDVTLVGLLD
VDGALFSADFRAPERFGQLYTQVSGRAGRATKPGTVLLQTHQSDNAMLRELMHKGYGEFARNQLFERTQALLPPAWHMIL
IRAEAHHAIDADNLLNQIGALLPQDEECEVIGPMPAPLDRKAGKYRRQLLFQTKNRNRLQLEFERALPQIEQLPLAKKCR
WSIDRDPQDLL

Sequences:

>Translated_731_residues
MPLFVEVALPVPMRQNFSYQVPEHITISPQKGMRVQVPFGRQQLIGLITATSDSCSLTPKQIKPIVAILDTEPLLPDSLY
KLTAWAARYYFCSLGQMLSQAIPVALRKGAEVKASTVSYWTLTEAGKEADINQLNRAPAQRKLLHSLRERDLELGEIASL
ELSKAAVKSLDEKGWIEKKSRTLDIDLSWRNELEMTEEPHRLNPEQAIAVATLTQQQGYHCTLLEGVTGSGKTEVYLALL
ETVLKQGKQALVLVPEIGLTPQTINRFRRRFNVKVAVIHSGLTDNQRLDAWRQAKSGEAAIIIGTRSALFTPMLYPGVII
LDEEHDSSFKQQEGVGYHARDLAVMRGHLEQIPVLLGTATPSLETLQNALNGRYQHLHLGKRAGAAEKVRQGIVDIRNQP
LRSGMSHALLNEMRIHLDAGNQVILFLNRRGFAPALICHECGHLHECDRCDAFFTVHQALGEIRCHHCSNQYAIPKQCHS
CGSTMLAGQGVGTEQLASFLEKEFPNHPVVRIDRDTTRNKGSLEGHLNAIHKGEYKILVGTQMLAKGHHFPDVTLVGLLD
VDGALFSADFRAPERFGQLYTQVSGRAGRATKPGTVLLQTHQSDNAMLRELMHKGYGEFARNQLFERTQALLPPAWHMIL
IRAEAHHAIDADNLLNQIGALLPQDEECEVIGPMPAPLDRKAGKYRRQLLFQTKNRNRLQLEFERALPQIEQLPLAKKCR
WSIDRDPQDLL
>Mature_730_residues
PLFVEVALPVPMRQNFSYQVPEHITISPQKGMRVQVPFGRQQLIGLITATSDSCSLTPKQIKPIVAILDTEPLLPDSLYK
LTAWAARYYFCSLGQMLSQAIPVALRKGAEVKASTVSYWTLTEAGKEADINQLNRAPAQRKLLHSLRERDLELGEIASLE
LSKAAVKSLDEKGWIEKKSRTLDIDLSWRNELEMTEEPHRLNPEQAIAVATLTQQQGYHCTLLEGVTGSGKTEVYLALLE
TVLKQGKQALVLVPEIGLTPQTINRFRRRFNVKVAVIHSGLTDNQRLDAWRQAKSGEAAIIIGTRSALFTPMLYPGVIIL
DEEHDSSFKQQEGVGYHARDLAVMRGHLEQIPVLLGTATPSLETLQNALNGRYQHLHLGKRAGAAEKVRQGIVDIRNQPL
RSGMSHALLNEMRIHLDAGNQVILFLNRRGFAPALICHECGHLHECDRCDAFFTVHQALGEIRCHHCSNQYAIPKQCHSC
GSTMLAGQGVGTEQLASFLEKEFPNHPVVRIDRDTTRNKGSLEGHLNAIHKGEYKILVGTQMLAKGHHFPDVTLVGLLDV
DGALFSADFRAPERFGQLYTQVSGRAGRATKPGTVLLQTHQSDNAMLRELMHKGYGEFARNQLFERTQALLPPAWHMILI
RAEAHHAIDADNLLNQIGALLPQDEECEVIGPMPAPLDRKAGKYRRQLLFQTKNRNRLQLEFERALPQIEQLPLAKKCRW
SIDRDPQDLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=733, Percent_Identity=51.4324693042292, Blast_Score=739, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81787; Mature: 81656

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLFVEVALPVPMRQNFSYQVPEHITISPQKGMRVQVPFGRQQLIGLITATSDSCSLTPK
CCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEEECCCCHHHEEEEEEECCCCCCCCHH
QIKPIVAILDTEPLLPDSLYKLTAWAARYYFCSLGQMLSQAIPVALRKGAEVKASTVSYW
HHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECEEEEE
TLTEAGKEADINQLNRAPAQRKLLHSLRERDLELGEIASLELSKAAVKSLDEKGWIEKKS
EEECCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
RTLDIDLSWRNELEMTEEPHRLNPEQAIAVATLTQQQGYHCTLLEGVTGSGKTEVYLALL
CEEEEEECCCCCCCCCCCCCCCCHHHHEEEEEEHHCCCCEEEEEECCCCCCCHHHHHHHH
ETVLKQGKQALVLVPEIGLTPQTINRFRRRFNVKVAVIHSGLTDNQRLDAWRQAKSGEAA
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCEE
IIIGTRSALFTPMLYPGVIILDEEHDSSFKQQEGVGYHARDLAVMRGHLEQIPVLLGTAT
EEEECCHHHHHHHHCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCEEEECCC
PSLETLQNALNGRYQHLHLGKRAGAAEKVRQGIVDIRNQPLRSGMSHALLNEMRIHLDAG
CCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECC
NQVILFLNRRGFAPALICHECGHLHECDRCDAFFTVHQALGEIRCHHCSNQYAIPKQCHS
CEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
CGSTMLAGQGVGTEQLASFLEKEFPNHPVVRIDRDTTRNKGSLEGHLNAIHKGEYKILVG
CCCCEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHEECCCEEEEEE
TQMLAKGHHFPDVTLVGLLDVDGALFSADFRAPERFGQLYTQVSGRAGRATKPGTVLLQT
HHHHHCCCCCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEE
HQSDNAMLRELMHKGYGEFARNQLFERTQALLPPAWHMILIRAEAHHAIDADNLLNQIGA
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHH
LLPQDEECEVIGPMPAPLDRKAGKYRRQLLFQTKNRNRLQLEFERALPQIEQLPLAKKCR
HCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCCHHHHCCCCHHHC
WSIDRDPQDLL
CCCCCCHHHCC
>Mature Secondary Structure 
PLFVEVALPVPMRQNFSYQVPEHITISPQKGMRVQVPFGRQQLIGLITATSDSCSLTPK
CEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEEECCCCHHHEEEEEEECCCCCCCCHH
QIKPIVAILDTEPLLPDSLYKLTAWAARYYFCSLGQMLSQAIPVALRKGAEVKASTVSYW
HHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECEEEEE
TLTEAGKEADINQLNRAPAQRKLLHSLRERDLELGEIASLELSKAAVKSLDEKGWIEKKS
EEECCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
RTLDIDLSWRNELEMTEEPHRLNPEQAIAVATLTQQQGYHCTLLEGVTGSGKTEVYLALL
CEEEEEECCCCCCCCCCCCCCCCHHHHEEEEEEHHCCCCEEEEEECCCCCCCHHHHHHHH
ETVLKQGKQALVLVPEIGLTPQTINRFRRRFNVKVAVIHSGLTDNQRLDAWRQAKSGEAA
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCEE
IIIGTRSALFTPMLYPGVIILDEEHDSSFKQQEGVGYHARDLAVMRGHLEQIPVLLGTAT
EEEECCHHHHHHHHCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCEEEECCC
PSLETLQNALNGRYQHLHLGKRAGAAEKVRQGIVDIRNQPLRSGMSHALLNEMRIHLDAG
CCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECC
NQVILFLNRRGFAPALICHECGHLHECDRCDAFFTVHQALGEIRCHHCSNQYAIPKQCHS
CEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
CGSTMLAGQGVGTEQLASFLEKEFPNHPVVRIDRDTTRNKGSLEGHLNAIHKGEYKILVG
CCCCEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHEECCCEEEEEE
TQMLAKGHHFPDVTLVGLLDVDGALFSADFRAPERFGQLYTQVSGRAGRATKPGTVLLQT
HHHHHCCCCCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEE
HQSDNAMLRELMHKGYGEFARNQLFERTQALLPPAWHMILIRAEAHHAIDADNLLNQIGA
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHH
LLPQDEECEVIGPMPAPLDRKAGKYRRQLLFQTKNRNRLQLEFERALPQIEQLPLAKKCR
HCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCCHHHHCCCCHHHC
WSIDRDPQDLL
CCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]