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The map label for this gene is gap3 [H]

Identifier: 157963548

GI number: 157963548

Start: 4541128

End: 4542141

Strand: Reverse

Name: gap3 [H]

Synonym: Spea_3736

Alternate gene names: 157963548

Gene position: 4542141-4541128 (Counterclockwise)

Preceding gene: 157963549

Following gene: 157963547

Centisome position: 87.78

GC content: 48.52

Gene sequence:

>1014_bases
ATGACAATCAAAATAGGTATCAACGGCTTCGGTAGAATGGGGCGTTTAGCGCTAAGGTCGGCATGGCACAATAGCGATGT
TGAATTTGTACAGATCAACGATCCGGCCGGTGATGCGGCGACATTGGCTCATTTGCTGACGTTCGATTCCATTCATGGTC
GTTGGTCAGAGGAAGCGACGGCCGACGGCGATAACATAGTGATTGGCGCAAGCCGCATTGTCACTACGCGTAATAAAGCG
ATTGCCGATACTGATTGGTCAGATTGTGACGTGGTGATTGAAGCGTCAGGTGTAATGAAGACCAAAGCCTTGCTACAAGC
CTATTTAGACCAAGGGGTGAAGAGAGTCGTGGTCACCGCGCCGGTGAAAGAAGAGGGCGTGCTCAATATCGTCATGGGGG
TCAATCAAGATCTTTATAACAAGGACATTCATCCGATTGTGACGGCAGCGTCTTGCACGACTAACTGTTTGGCGCCTGTG
GTTAAGGTGATCCACGAAAACATTGGTATCAAGCATGGGTCGATGACAACCATTCACGATATTACCAATACCCAGACCAT
CTTAGATGCGCCTCATAAAGACTTAAGACGCGCTCGTGCCTGTGGTTTAAGCCTTATTCCAACTACAACAGGCAGTGCTA
CGGCCATTACCCATATCTTCCCAGAACTAAAGGGTAAGTTAAATGGTCATGCTGTGCGTGTTCCTTTGGCTAATGCCTCT
ATTACAGATTGTGTATTCGAGCTTAACCGCGAAACCACCGAAGCTGAAGTAAACCAGTTGCTTAAGCAAGCGGCGGAAGG
GCAGCTAAAAGGCATTCTAGGTTATGAAGAGCGCCCATTAGTATCTGTGGATTACAAGACAGACCCGCGTTCGAGCATCA
TAGATGCGCCATCGACCATGGTCGTTAACGGTACCCAAGTTAAACTCTACGTCTGGTATGACAATGAGTGGGGCTATGCA
AACCGCACTGCCGAGCTAGCGATTATGGTTGGACGTATAGACCAAGCAGAGTAA

Upstream 100 bases:

>100_bases
GAGCTTATGCTCTGAGCATCAAATCCGGCTATTACATTGAGAAATAAATTCTAACTCTAGATTGAACTTAAACGATTTAA
TAACAGATTAAGAGACGACT

Downstream 100 bases:

>100_bases
CGCTAAGACGATGCAAGCTGGGACGGTTAAAGTCGGGAAAGCTGAGGCTGTCAGCTGTTCTAGCCTTTTCTTTTTCCTAG
CAGCGTAGCGTCCTAGTAGT

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTIKIGINGFGRMGRLALRSAWHNSDVEFVQINDPAGDAATLAHLLTFDSIHGRWSEEATADGDNIVIGASRIVTTRNKA
IADTDWSDCDVVIEASGVMKTKALLQAYLDQGVKRVVVTAPVKEEGVLNIVMGVNQDLYNKDIHPIVTAASCTTNCLAPV
VKVIHENIGIKHGSMTTIHDITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
ITDCVFELNRETTEAEVNQLLKQAAEGQLKGILGYEERPLVSVDYKTDPRSSIIDAPSTMVVNGTQVKLYVWYDNEWGYA
NRTAELAIMVGRIDQAE

Sequences:

>Translated_337_residues
MTIKIGINGFGRMGRLALRSAWHNSDVEFVQINDPAGDAATLAHLLTFDSIHGRWSEEATADGDNIVIGASRIVTTRNKA
IADTDWSDCDVVIEASGVMKTKALLQAYLDQGVKRVVVTAPVKEEGVLNIVMGVNQDLYNKDIHPIVTAASCTTNCLAPV
VKVIHENIGIKHGSMTTIHDITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
ITDCVFELNRETTEAEVNQLLKQAAEGQLKGILGYEERPLVSVDYKTDPRSSIIDAPSTMVVNGTQVKLYVWYDNEWGYA
NRTAELAIMVGRIDQAE
>Mature_336_residues
TIKIGINGFGRMGRLALRSAWHNSDVEFVQINDPAGDAATLAHLLTFDSIHGRWSEEATADGDNIVIGASRIVTTRNKAI
ADTDWSDCDVVIEASGVMKTKALLQAYLDQGVKRVVVTAPVKEEGVLNIVMGVNQDLYNKDIHPIVTAASCTTNCLAPVV
KVIHENIGIKHGSMTTIHDITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANASI
TDCVFELNRETTEAEVNQLLKQAAEGQLKGILGYEERPLVSVDYKTDPRSSIIDAPSTMVVNGTQVKLYVWYDNEWGYAN
RTAELAIMVGRIDQAE

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=327, Percent_Identity=41.8960244648318, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI7657116, Length=327, Percent_Identity=39.4495412844037, Blast_Score=239, Evalue=3e-63,
Organism=Escherichia coli, GI1788079, Length=329, Percent_Identity=42.2492401215805, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1789295, Length=327, Percent_Identity=39.4495412844037, Blast_Score=247, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI17534677, Length=330, Percent_Identity=40.9090909090909, Blast_Score=261, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI17534679, Length=330, Percent_Identity=40.3030303030303, Blast_Score=258, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI32566163, Length=330, Percent_Identity=40, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17568413, Length=330, Percent_Identity=40, Blast_Score=252, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321631, Length=333, Percent_Identity=42.9429429429429, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6322468, Length=333, Percent_Identity=42.6426426426426, Blast_Score=282, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6322409, Length=333, Percent_Identity=41.7417417417417, Blast_Score=267, Evalue=2e-72,
Organism=Drosophila melanogaster, GI19922412, Length=336, Percent_Identity=40.1785714285714, Blast_Score=262, Evalue=2e-70,
Organism=Drosophila melanogaster, GI85725000, Length=326, Percent_Identity=40.7975460122699, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI22023983, Length=326, Percent_Identity=40.7975460122699, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI17933600, Length=326, Percent_Identity=40.4907975460123, Blast_Score=250, Evalue=9e-67,
Organism=Drosophila melanogaster, GI18110149, Length=326, Percent_Identity=40.4907975460123, Blast_Score=250, Evalue=9e-67,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36638; Mature: 36507

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKIGINGFGRMGRLALRSAWHNSDVEFVQINDPAGDAATLAHLLTFDSIHGRWSEEAT
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
ADGDNIVIGASRIVTTRNKAIADTDWSDCDVVIEASGVMKTKALLQAYLDQGVKRVVVTA
CCCCEEEEECCEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEC
PVKEEGVLNIVMGVNQDLYNKDIHPIVTAASCTTNCLAPVVKVIHENIGIKHGSMTTIHD
CCCCCCEEEEEEECCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
CCCCCHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
ITDCVFELNRETTEAEVNQLLKQAAEGQLKGILGYEERPLVSVDYKTDPRSSIIDAPSTM
HHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCHHHCCCCCEE
VVNGTQVKLYVWYDNEWGYANRTAELAIMVGRIDQAE
EEECCEEEEEEEECCCCCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
TIKIGINGFGRMGRLALRSAWHNSDVEFVQINDPAGDAATLAHLLTFDSIHGRWSEEAT
EEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
ADGDNIVIGASRIVTTRNKAIADTDWSDCDVVIEASGVMKTKALLQAYLDQGVKRVVVTA
CCCCEEEEECCEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEC
PVKEEGVLNIVMGVNQDLYNKDIHPIVTAASCTTNCLAPVVKVIHENIGIKHGSMTTIHD
CCCCCCEEEEEEECCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
CCCCCHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
ITDCVFELNRETTEAEVNQLLKQAAEGQLKGILGYEERPLVSVDYKTDPRSSIIDAPSTM
HHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCHHHCCCCCEE
VVNGTQVKLYVWYDNEWGYANRTAELAIMVGRIDQAE
EEECCEEEEEEEECCCCCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8378350 [H]