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The map label for this gene is caiD [C]

Identifier: 157963521

GI number: 157963521

Start: 4515241

End: 4516047

Strand: Reverse

Name: caiD [C]

Synonym: Spea_3709

Alternate gene names: 157963521

Gene position: 4516047-4515241 (Counterclockwise)

Preceding gene: 157963524

Following gene: 157963520

Centisome position: 87.27

GC content: 45.23

Gene sequence:

>807_bases
ATGACTGATGATTTAGTACGTATTACCAAGGAGAATGGCATTGCTTTTGTGAGTCTTAATCGGCCAGATAAGTATAATGC
ATTGAATTACGAGCTTTTTAAGCAGATTGATCGCTGCATAAAAACACTAGCGCGAGACAAAACGCTTACTGCCGTAGTGC
TTTTTGGTGAAGGTGGTAATTTCAGCTCAGGCTTGGATGTAAAAAGTGTGATGAGCTCGCCGTTAGACGCGGTTAAATTA
CTGTTTAAGTTTCTTCCTGGTAACGCCAATCTCGCCCAGCGCGTTTCTATAGGATGGCGACGATTACCCGTTCCCGTTAT
CGCGGTGCTAGAGGGGATTTGTTATGGCGGCGGCATGCAAATAGTCTTAGGTGCCGATTTTAGAATCGCTGCACCAGATT
GCAAGATGTCGATAATGGAAGCTAAATGGGGGCTCGTGCCTGATATGGCTGGCTTAGCGGGCTTGCGAGAGTTAATGCCA
AAAGATCAGGCGCTTATGTTAACAATGTCGGCAGATGTTATCTCTGCTGAAAAGGCCTTATCCATGAATTTGATTACGGC
TGTGGAAGATAATCCTATGGCCGCAGCTAGCAAATTGGCGAGTAAGTTATCGCAAACCTCTCCCGATGCCAATGCGGCAA
TTAAGCGCAGTATAAATAAGAGTTGGTCGGCTCCCGTTCGACTGCTGTTAGGGCGTGAGTCTTGGTATCAGGTGCGCTTA
TTATTGGGAAAAAATCGAGTGATTGCAGCATTAAGACAGACTAAGAAACCAGATAAAACTTATAGAAACAGGCAATCAGG
CTGGTAA

Upstream 100 bases:

>100_bases
AATGTCATAATTCCTACGTATGTTATAGCTATTAACGAAATATTCCAAATGTGTTAACTTTGCTTTCTGGACAGACCACT
TGGCAAGGAAGGACGCGAAT

Downstream 100 bases:

>100_bases
TTTTTAGCTAAGATGATGAAGTGTGACCAGGTTCACGAATTGTTGTGAATTTTCTGTAGTAGGCAAGGGCTTATTCTACA
CTCCCGCCTATTCTAAGGTT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTDDLVRITKENGIAFVSLNRPDKYNALNYELFKQIDRCIKTLARDKTLTAVVLFGEGGNFSSGLDVKSVMSSPLDAVKL
LFKFLPGNANLAQRVSIGWRRLPVPVIAVLEGICYGGGMQIVLGADFRIAAPDCKMSIMEAKWGLVPDMAGLAGLRELMP
KDQALMLTMSADVISAEKALSMNLITAVEDNPMAAASKLASKLSQTSPDANAAIKRSINKSWSAPVRLLLGRESWYQVRL
LLGKNRVIAALRQTKKPDKTYRNRQSGW

Sequences:

>Translated_268_residues
MTDDLVRITKENGIAFVSLNRPDKYNALNYELFKQIDRCIKTLARDKTLTAVVLFGEGGNFSSGLDVKSVMSSPLDAVKL
LFKFLPGNANLAQRVSIGWRRLPVPVIAVLEGICYGGGMQIVLGADFRIAAPDCKMSIMEAKWGLVPDMAGLAGLRELMP
KDQALMLTMSADVISAEKALSMNLITAVEDNPMAAASKLASKLSQTSPDANAAIKRSINKSWSAPVRLLLGRESWYQVRL
LLGKNRVIAALRQTKKPDKTYRNRQSGW
>Mature_267_residues
TDDLVRITKENGIAFVSLNRPDKYNALNYELFKQIDRCIKTLARDKTLTAVVLFGEGGNFSSGLDVKSVMSSPLDAVKLL
FKFLPGNANLAQRVSIGWRRLPVPVIAVLEGICYGGGMQIVLGADFRIAAPDCKMSIMEAKWGLVPDMAGLAGLRELMPK
DQALMLTMSADVISAEKALSMNLITAVEDNPMAAASKLASKLSQTSPDANAAIKRSINKSWSAPVRLLLGRESWYQVRLL
LGKNRVIAALRQTKKPDKTYRNRQSGW

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=190, Percent_Identity=31.0526315789474, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI213417737, Length=216, Percent_Identity=24.537037037037, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI157694516, Length=216, Percent_Identity=24.537037037037, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI68989263, Length=232, Percent_Identity=28.0172413793103, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI194097323, Length=216, Percent_Identity=26.3888888888889, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI31542718, Length=197, Percent_Identity=27.9187817258883, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787660, Length=230, Percent_Identity=30.4347826086957, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI221142681, Length=273, Percent_Identity=26.7399267399267, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=25.968992248062, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790281, Length=178, Percent_Identity=28.0898876404494, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788682, Length=215, Percent_Identity=27.4418604651163, Blast_Score=67, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=217, Percent_Identity=30.8755760368664, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=262, Percent_Identity=24.0458015267176, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=210, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=210, Percent_Identity=25.2380952380952, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=220, Percent_Identity=25.4545454545455, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=256, Percent_Identity=21.484375, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=192, Percent_Identity=26.0416666666667, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=191, Percent_Identity=27.7486910994764, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20129971, Length=227, Percent_Identity=24.6696035242291, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653477, Length=227, Percent_Identity=24.6696035242291, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=26.171875, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21357171, Length=259, Percent_Identity=21.2355212355212, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29386; Mature: 29255

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDLVRITKENGIAFVSLNRPDKYNALNYELFKQIDRCIKTLARDKTLTAVVLFGEGGN
CCCCCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
FSSGLDVKSVMSSPLDAVKLLFKFLPGNANLAQRVSIGWRRLPVPVIAVLEGICYGGGMQ
CCCCCCHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCEE
IVLGADFRIAAPDCKMSIMEAKWGLVPDMAGLAGLRELMPKDQALMLTMSADVISAEKAL
EEEECCEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
SMNLITAVEDNPMAAASKLASKLSQTSPDANAAIKRSINKSWSAPVRLLLGRESWYQVRL
HHEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEECCCCCEEEEE
LLGKNRVIAALRQTKKPDKTYRNRQSGW
ECCCCHHHHHHHHCCCCCHHHHCCCCCC
>Mature Secondary Structure 
TDDLVRITKENGIAFVSLNRPDKYNALNYELFKQIDRCIKTLARDKTLTAVVLFGEGGN
CCCCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
FSSGLDVKSVMSSPLDAVKLLFKFLPGNANLAQRVSIGWRRLPVPVIAVLEGICYGGGMQ
CCCCCCHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCEE
IVLGADFRIAAPDCKMSIMEAKWGLVPDMAGLAGLRELMPKDQALMLTMSADVISAEKAL
EEEECCEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
SMNLITAVEDNPMAAASKLASKLSQTSPDANAAIKRSINKSWSAPVRLLLGRESWYQVRL
HHEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEECCCCCEEEEE
LLGKNRVIAALRQTKKPDKTYRNRQSGW
ECCCCHHHHHHHHCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA