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The map label for this gene is btuB [H]

Identifier: 157963345

GI number: 157963345

Start: 4313691

End: 4315727

Strand: Reverse

Name: btuB [H]

Synonym: Spea_3531

Alternate gene names: 157963345

Gene position: 4315727-4313691 (Counterclockwise)

Preceding gene: 157963346

Following gene: 157963344

Centisome position: 83.4

GC content: 43.15

Gene sequence:

>2037_bases
GTGATCAGAACAAGAAATACAAAAATAGCAGTTATCATCAGCAGTTTATTGTCGTGCCCTGCTGCAATGGCTAATGAAGC
AAACGATGTTTTTTCACTTGGTGAAATCGTCGTGTCAGACCAAACAGGTGTTAAAGATATTGCTATCAACAACACTATGA
CCGCAGAGCAAATTGAGTTAATTGGTGCAAAGACTGCGGCTGATGCACTCGACTATGTCCCTGGAGTACATGTTGCTCAA
TCGAGTAAAGGTGAGAAATTTCTGACCATTCAAGGGTTTGAGCAAGATAAAGTGCTTATTTTACTTGATGGTGTGCCTTA
CTATGAAACCAACTACGGTCAGCTAGATCTTAATCAAATTCCAGCGAGCATTATTGCCAAGATCGAGGTTGTTAAAGGTG
CTTCATCTGTACTTTATGGACCTAATGGCATGGGTGGCGTCGTCAATATTATCACCAAGAAAGGTCAAGATGGTGTTAGT
GGTGATGTTCAGGCTTCATTCGGGCAGATGGGCCAGAATCATGAGTCAGCTTCACTTAGCCTAGGCAAAAATGGCTTTAG
CTTATTCGCAACTGTTGATCATCGTGGTCGTGATGCGATGCGCATGTCTAATGATTTCGAGCCTGTTTTATCTGACATTA
GAGATAAATATGGCGACTTGCCAAAGCAGATGGTTGTTGAAGATGGCGGTAAGCGCGAAAACTCAGCCTACGAGTCGACT
AACATCTGGGTCCGTGGTGGTTACGCCAACGAAAGTACCGAACTATTTGCTAGTGTTTTTAGTTTAGATTCAGATCGCGA
ATTGCCATACAACACCCGTAATAACAAGGTGTTTAGTGACTTTAGTAGTTTTGCTGATATCTCTGAATACAAAGATACCG
GTATGGATTTAAGCGGTTTACACCGCGTTAATGATTGGCTAACAGTGCGTGCTTTAGGTTACTACCACATGCATAACGAT
AGCTATGACTCCTATAGCAATCCTGAGAAAGAGGTAAAACTTGCGACCAGTAGCTTTAGCGATTATACCGCTGGTGGCGC
TCTGTTTACCGATATGGCGCTAGCCGATTGGAACAGTTTAAGTCTTTCTGTGAACTTTAAGAATGACGTTCATAAGAAGA
AGAATGTTGCCTATGTGGATCCTGACCGCTCTCACGCTTATGAGCGTTCTCAGCTTGATACCATTAGCTTGGCCGCTGAA
GATACCATGACATTTGGTGCTTTAAATGTGGTTGCAGGTGTTGCATGGCATAAACAAACGGTGGTTGAAGACCGTGGCAA
CGATGCGGCACCGGGTGAAGATGGCTCGGATACACTCGATCCAATGTTAGGTATGAGCTACACCTTCGATAACGCAGGTT
TAGTTTATGGCTCTATTGCTCAAAAGACTCGCTTTGCGACTTTTACTGAGATGTATGACGACACTGGTGCGCGCCACGAT
ATTAAACCTGAGCGTAATACTAGCTATACCTTAGGCTGGAAGAATGATTTTAGCCATAAATTATTAAACAGTATCGATAT
CGGCCTGTTCTACCATGATGTGAGCGATAAGATTGTCTCTACTTATATGCCTGATCCAACGGATCCAGGTTGGGACCTTG
AAATCTACCAAAACATTGGTAAGTCAGAGTTTTACGGTATCGAGATCACTACCTTAAGTCAGCTAACAGAAAATATCAGC
TTATCTCTGGATTACACTTATACCCATGCACGTAACAAGAGTGTTGACCGTGATTCGGATTACTTCCGTGACATTCCAAA
AGACTCTGTTACTGCGATCGTTAACTGGTATCAGCCTTGGTTAGATATTGATAGTAACTTACGCGTTCGCTACCGCACTG
ACATCATGATTGATGAGCGTTCAGATGAGTGGGAGTCAGATACGTTAACTATTGATATGGGTATTAAGAAATCGATTACA
CCGCAGCTTTCTGCTTACGTTAATCTAAACAACCTACTCGATGAGTCGCACTACGAAGGATACGGTCAGCCAAACGAAGG
CCGCTCTTATGAAGTGGGCGTAAAGTACCGCTTCTAA

Upstream 100 bases:

>100_bases
AACAGCAGATTAAGACTAAGATACGCGCGAATTTATGACTAGCTAACACTTTCGTATGGAAGTTTGCTTAGTCACTATAA
CGAGCAGATCGGAGTCTTAG

Downstream 100 bases:

>100_bases
GGAGTCAGAATGCTAAACAGACTATTTAGTGCTGTTGTCATTCTCCTTTGTGGTCTTTCAAGTGTATCGGCAGCGCCGAT
ACACTTGGTCGATCAACAAG

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MIRTRNTKIAVIISSLLSCPAAMANEANDVFSLGEIVVSDQTGVKDIAINNTMTAEQIELIGAKTAADALDYVPGVHVAQ
SSKGEKFLTIQGFEQDKVLILLDGVPYYETNYGQLDLNQIPASIIAKIEVVKGASSVLYGPNGMGGVVNIITKKGQDGVS
GDVQASFGQMGQNHESASLSLGKNGFSLFATVDHRGRDAMRMSNDFEPVLSDIRDKYGDLPKQMVVEDGGKRENSAYEST
NIWVRGGYANESTELFASVFSLDSDRELPYNTRNNKVFSDFSSFADISEYKDTGMDLSGLHRVNDWLTVRALGYYHMHND
SYDSYSNPEKEVKLATSSFSDYTAGGALFTDMALADWNSLSLSVNFKNDVHKKKNVAYVDPDRSHAYERSQLDTISLAAE
DTMTFGALNVVAGVAWHKQTVVEDRGNDAAPGEDGSDTLDPMLGMSYTFDNAGLVYGSIAQKTRFATFTEMYDDTGARHD
IKPERNTSYTLGWKNDFSHKLLNSIDIGLFYHDVSDKIVSTYMPDPTDPGWDLEIYQNIGKSEFYGIEITTLSQLTENIS
LSLDYTYTHARNKSVDRDSDYFRDIPKDSVTAIVNWYQPWLDIDSNLRVRYRTDIMIDERSDEWESDTLTIDMGIKKSIT
PQLSAYVNLNNLLDESHYEGYGQPNEGRSYEVGVKYRF

Sequences:

>Translated_678_residues
MIRTRNTKIAVIISSLLSCPAAMANEANDVFSLGEIVVSDQTGVKDIAINNTMTAEQIELIGAKTAADALDYVPGVHVAQ
SSKGEKFLTIQGFEQDKVLILLDGVPYYETNYGQLDLNQIPASIIAKIEVVKGASSVLYGPNGMGGVVNIITKKGQDGVS
GDVQASFGQMGQNHESASLSLGKNGFSLFATVDHRGRDAMRMSNDFEPVLSDIRDKYGDLPKQMVVEDGGKRENSAYEST
NIWVRGGYANESTELFASVFSLDSDRELPYNTRNNKVFSDFSSFADISEYKDTGMDLSGLHRVNDWLTVRALGYYHMHND
SYDSYSNPEKEVKLATSSFSDYTAGGALFTDMALADWNSLSLSVNFKNDVHKKKNVAYVDPDRSHAYERSQLDTISLAAE
DTMTFGALNVVAGVAWHKQTVVEDRGNDAAPGEDGSDTLDPMLGMSYTFDNAGLVYGSIAQKTRFATFTEMYDDTGARHD
IKPERNTSYTLGWKNDFSHKLLNSIDIGLFYHDVSDKIVSTYMPDPTDPGWDLEIYQNIGKSEFYGIEITTLSQLTENIS
LSLDYTYTHARNKSVDRDSDYFRDIPKDSVTAIVNWYQPWLDIDSNLRVRYRTDIMIDERSDEWESDTLTIDMGIKKSIT
PQLSAYVNLNNLLDESHYEGYGQPNEGRSYEVGVKYRF
>Mature_678_residues
MIRTRNTKIAVIISSLLSCPAAMANEANDVFSLGEIVVSDQTGVKDIAINNTMTAEQIELIGAKTAADALDYVPGVHVAQ
SSKGEKFLTIQGFEQDKVLILLDGVPYYETNYGQLDLNQIPASIIAKIEVVKGASSVLYGPNGMGGVVNIITKKGQDGVS
GDVQASFGQMGQNHESASLSLGKNGFSLFATVDHRGRDAMRMSNDFEPVLSDIRDKYGDLPKQMVVEDGGKRENSAYEST
NIWVRGGYANESTELFASVFSLDSDRELPYNTRNNKVFSDFSSFADISEYKDTGMDLSGLHRVNDWLTVRALGYYHMHND
SYDSYSNPEKEVKLATSSFSDYTAGGALFTDMALADWNSLSLSVNFKNDVHKKKNVAYVDPDRSHAYERSQLDTISLAAE
DTMTFGALNVVAGVAWHKQTVVEDRGNDAAPGEDGSDTLDPMLGMSYTFDNAGLVYGSIAQKTRFATFTEMYDDTGARHD
IKPERNTSYTLGWKNDFSHKLLNSIDIGLFYHDVSDKIVSTYMPDPTDPGWDLEIYQNIGKSEFYGIEITTLSQLTENIS
LSLDYTYTHARNKSVDRDSDYFRDIPKDSVTAIVNWYQPWLDIDSNLRVRYRTDIMIDERSDEWESDTLTIDMGIKKSIT
PQLSAYVNLNNLLDESHYEGYGQPNEGRSYEVGVKYRF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=161, Percent_Identity=32.2981366459627, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1790405, Length=125, Percent_Identity=30.4, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 75508; Mature: 75508

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTRNTKIAVIISSLLSCPAAMANEANDVFSLGEIVVSDQTGVKDIAINNTMTAEQIEL
CCCCCCCCHHHHHHHHHHCCHHHHCCCCCCEEECEEEEECCCCCEEEEECCCCCHHHHHH
IGAKTAADALDYVPGVHVAQSSKGEKFLTIQGFEQDKVLILLDGVPYYETNYGQLDLNQI
HCCHHHHHHHHHCCCCEEECCCCCCEEEEEECCCCCCEEEEECCCCCEECCCCEEEHHHC
PASIIAKIEVVKGASSVLYGPNGMGGVVNIITKKGQDGVSGDVQASFGQMGQNHESASLS
CHHHHHEEEEECCCCCEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCCCCEEE
LGKNGFSLFATVDHRGRDAMRMSNDFEPVLSDIRDKYGDLPKQMVVEDGGKRENSAYEST
ECCCCEEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCC
NIWVRGGYANESTELFASVFSLDSDRELPYNTRNNKVFSDFSSFADISEYKDTGMDLSGL
EEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHH
HRVNDWLTVRALGYYHMHNDSYDSYSNPEKEVKLATSSFSDYTAGGALFTDMALADWNSL
HHHCCEEEEEEEEEEEEECCCCCCCCCCCHHEEEEECCCCCCCCCCHHHHHHHHCCCCEE
SLSVNFKNDVHKKKNVAYVDPDRSHAYERSQLDTISLAAEDTMTFGALNVVAGVAWHKQT
EEEEECCCCCHHCCCEEEECCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
VVEDRGNDAAPGEDGSDTLDPMLGMSYTFDNAGLVYGSIAQKTRFATFTEMYDDTGARHD
HHHCCCCCCCCCCCCCCHHHHHHCCEEEECCCCEEEECHHHHHHHHHHHHHHHCCCCCCC
IKPERNTSYTLGWKNDFSHKLLNSIDIGLFYHDVSDKIVSTYMPDPTDPGWDLEIYQNIG
CCCCCCCEEEEECCCCHHHHHHHHCEEEEEEECHHHHHHHHCCCCCCCCCCCHHHHHCCC
KSEFYGIEITTLSQLTENISLSLDYTYTHARNKSVDRDSDYFRDIPKDSVTAIVNWYQPW
CCEEEEEEEEEHHHHHCCEEEEEEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
LDIDSNLRVRYRTDIMIDERSDEWESDTLTIDMGIKKSITPQLSAYVNLNNLLDESHYEG
CCCCCCCEEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEEEEHHHHHCCCCCCC
YGQPNEGRSYEVGVKYRF
CCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MIRTRNTKIAVIISSLLSCPAAMANEANDVFSLGEIVVSDQTGVKDIAINNTMTAEQIEL
CCCCCCCCHHHHHHHHHHCCHHHHCCCCCCEEECEEEEECCCCCEEEEECCCCCHHHHHH
IGAKTAADALDYVPGVHVAQSSKGEKFLTIQGFEQDKVLILLDGVPYYETNYGQLDLNQI
HCCHHHHHHHHHCCCCEEECCCCCCEEEEEECCCCCCEEEEECCCCCEECCCCEEEHHHC
PASIIAKIEVVKGASSVLYGPNGMGGVVNIITKKGQDGVSGDVQASFGQMGQNHESASLS
CHHHHHEEEEECCCCCEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCCCCEEE
LGKNGFSLFATVDHRGRDAMRMSNDFEPVLSDIRDKYGDLPKQMVVEDGGKRENSAYEST
ECCCCEEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCC
NIWVRGGYANESTELFASVFSLDSDRELPYNTRNNKVFSDFSSFADISEYKDTGMDLSGL
EEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHH
HRVNDWLTVRALGYYHMHNDSYDSYSNPEKEVKLATSSFSDYTAGGALFTDMALADWNSL
HHHCCEEEEEEEEEEEEECCCCCCCCCCCHHEEEEECCCCCCCCCCHHHHHHHHCCCCEE
SLSVNFKNDVHKKKNVAYVDPDRSHAYERSQLDTISLAAEDTMTFGALNVVAGVAWHKQT
EEEEECCCCCHHCCCEEEECCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
VVEDRGNDAAPGEDGSDTLDPMLGMSYTFDNAGLVYGSIAQKTRFATFTEMYDDTGARHD
HHHCCCCCCCCCCCCCCHHHHHHCCEEEECCCCEEEECHHHHHHHHHHHHHHHCCCCCCC
IKPERNTSYTLGWKNDFSHKLLNSIDIGLFYHDVSDKIVSTYMPDPTDPGWDLEIYQNIG
CCCCCCCEEEEECCCCHHHHHHHHCEEEEEEECHHHHHHHHCCCCCCCCCCCHHHHHCCC
KSEFYGIEITTLSQLTENISLSLDYTYTHARNKSVDRDSDYFRDIPKDSVTAIVNWYQPW
CCEEEEEEEEEHHHHHCCEEEEEEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
LDIDSNLRVRYRTDIMIDERSDEWESDTLTIDMGIKKSITPQLSAYVNLNNLLDESHYEG
CCCCCCCEEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEEEEHHHHHCCCCCCC
YGQPNEGRSYEVGVKYRF
CCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA