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The map label for this gene is cobS [H]

Identifier: 157963194

GI number: 157963194

Start: 4126933

End: 4127712

Strand: Reverse

Name: cobS [H]

Synonym: Spea_3379

Alternate gene names: 157963194

Gene position: 4127712-4126933 (Counterclockwise)

Preceding gene: 157963195

Following gene: 157963193

Centisome position: 79.77

GC content: 50.77

Gene sequence:

>780_bases
ATGCAAGCTTGGCAACAACAGCTCAATCTTTTCTTTATCGCTATGGGGTTCTTTACGCGGATCCCTATGCCGAAATGGAT
TGAGGTCGATGCAGATAAACTGAATAAGGCGAGCCGCTACTTTGGTCTCGTTGGCTTATTGGTCGGTGCCATTAGTGCGC
TGGTTTACACCTTAATGTTGTATTGGGTATCTCCCTCTATCGCCATTGTGCTGGCGATGATCACTAGCGTGCTAGTCACG
GGGGGCTTTCATGAAGACGGCCTTGCCGATACCGCCGATGGATTAGGTGGCGGCTGGACGGTTGAAGCCAAGCTTAACAT
CATGAAAGACTCTCGCCTTGGCAGTTATGGTGCGCTGGCTTTGGTGCTGGCACTGCTACTCAAGTGGCAACTACTAACAG
AGCTTGCGCTGTTTGACCCAAGCAGTGTCAGCCTTGCACTGATTGTCGGCCACTGTTTGAGTAGAGTGGTAGCTGCGAGC
TTTATCTTCTCCGAGCCCTATGTCAGTGACTCTGATACCAGCAAGAGCAAGCCACTTGCGCAAGAGCAGGGCATCAATGA
GCTATCTATCTTGCTGGCAACTGGCATATTGGCGCTGTTGTTAGTGGGGGTTATGCAAGCCTTAGTGTTAACGCTTGCTC
TTACCATAGTACGCTACGGTTTTGTCAGACTGTTTACAAAGCAGATTGGCGGTTATACCGGTGATACGCTCGGCGCGGCC
CAGCAAGGCTCTGAGCTGACCTGTTATCTGCTATTGCTTGTATTAGGAGTGAGCTGGTGA

Upstream 100 bases:

>100_bases
GATCGAAGACCGTTCGTCTTAGCTCTACTCGGCTTCTAATCGTCTCTGCTATTTTCGGATTTTTTGTCCTAGCTCTATTC
GATTTTTTAAGGATTTTTAC

Downstream 100 bases:

>100_bases
TCCATCTGGTTTTAGGCGGTGCTCGTAGCGGCAAGAGCCGCTTCGCCGAGGCGCAAGTACAGGCGTACTTAGCTAACGTC
ACAGTCAATAATGGCTCTGA

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MQAWQQQLNLFFIAMGFFTRIPMPKWIEVDADKLNKASRYFGLVGLLVGAISALVYTLMLYWVSPSIAIVLAMITSVLVT
GGFHEDGLADTADGLGGGWTVEAKLNIMKDSRLGSYGALALVLALLLKWQLLTELALFDPSSVSLALIVGHCLSRVVAAS
FIFSEPYVSDSDTSKSKPLAQEQGINELSILLATGILALLLVGVMQALVLTLALTIVRYGFVRLFTKQIGGYTGDTLGAA
QQGSELTCYLLLLVLGVSW

Sequences:

>Translated_259_residues
MQAWQQQLNLFFIAMGFFTRIPMPKWIEVDADKLNKASRYFGLVGLLVGAISALVYTLMLYWVSPSIAIVLAMITSVLVT
GGFHEDGLADTADGLGGGWTVEAKLNIMKDSRLGSYGALALVLALLLKWQLLTELALFDPSSVSLALIVGHCLSRVVAAS
FIFSEPYVSDSDTSKSKPLAQEQGINELSILLATGILALLLVGVMQALVLTLALTIVRYGFVRLFTKQIGGYTGDTLGAA
QQGSELTCYLLLLVLGVSW
>Mature_259_residues
MQAWQQQLNLFFIAMGFFTRIPMPKWIEVDADKLNKASRYFGLVGLLVGAISALVYTLMLYWVSPSIAIVLAMITSVLVT
GGFHEDGLADTADGLGGGWTVEAKLNIMKDSRLGSYGALALVLALLLKWQLLTELALFDPSSVSLALIVGHCLSRVVAAS
FIFSEPYVSDSDTSKSKPLAQEQGINELSILLATGILALLLVGVMQALVLTLALTIVRYGFVRLFTKQIGGYTGDTLGAA
QQGSELTCYLLLLVLGVSW

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=34.412955465587, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27896; Mature: 27896

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAWQQQLNLFFIAMGFFTRIPMPKWIEVDADKLNKASRYFGLVGLLVGAISALVYTLML
CCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YWVSPSIAIVLAMITSVLVTGGFHEDGLADTADGLGGGWTVEAKLNIMKDSRLGSYGALA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEHHCCCCCCHHHHH
LVLALLLKWQLLTELALFDPSSVSLALIVGHCLSRVVAASFIFSEPYVSDSDTSKSKPLA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
QEQGINELSILLATGILALLLVGVMQALVLTLALTIVRYGFVRLFTKQIGGYTGDTLGAA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
QQGSELTCYLLLLVLGVSW
HCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQAWQQQLNLFFIAMGFFTRIPMPKWIEVDADKLNKASRYFGLVGLLVGAISALVYTLML
CCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YWVSPSIAIVLAMITSVLVTGGFHEDGLADTADGLGGGWTVEAKLNIMKDSRLGSYGALA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEHHCCCCCCHHHHH
LVLALLLKWQLLTELALFDPSSVSLALIVGHCLSRVVAASFIFSEPYVSDSDTSKSKPLA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
QEQGINELSILLATGILALLLVGVMQALVLTLALTIVRYGFVRLFTKQIGGYTGDTLGAA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
QQGSELTCYLLLLVLGVSW
HCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA