Click here to switch to the map view.

The map label for this gene is glnG [H]

Identifier: 157963176

GI number: 157963176

Start: 4104098

End: 4105627

Strand: Reverse

Name: glnG [H]

Synonym: Spea_3361

Alternate gene names: 157963176

Gene position: 4105627-4104098 (Counterclockwise)

Preceding gene: 157963177

Following gene: 157963175

Centisome position: 79.34

GC content: 49.15

Gene sequence:

>1530_bases
ATGGAAACCATACTGATTGTCGATGATAACCAAGCCGTGTGTAATGCCTTAGCGTTAATGCTAGAGCTGAGCGGTTATCA
AACACTTACCTGCTTATCGCCTGACGTCGCCCTCGAACTCATCCGGTTGCATGACGTGGCGCTGGTGATACAAGATATGA
ACTTCACCCAAGACACCACCTCAGGTGAAGAGGGGCGTAATCTATTTTATGGCTTTAGACAGCTGCAGCCAGAGCTGCCT
ATCATATTGCTCACCGCTTGGACCCAGCTTGAACTTGCCGTGGAGCTAGTCAAAGAGGGCGCCGCCGATTACATGGGCAA
ACCTTGGGATGATGCCAAGTTGCTCAACAGCATCAGCAACCTTATTTCACTGCATCGACTGTCTAAGAAAAATGCTCAGT
TAAGCCGAGTTGAAAGCCAGCGTATGGTGGCCATTAAAGACGCGGAACTGTGTGGTATCGTTTTTAACAGTGGCGCCATG
CAGCGCTGTGTGGATTTAGCCCTGCAGATCGCAAAATCTGATGTGTCGGTGTTGATTACAGGCCCCAACGGCGCGGGTAA
GGATAAATTAGCCGATATCATTCACGCTAATTCTCCCTTGAGGTACAAGCCCTTTATCAAGGTCAATATTGGCGCGTTGC
CGATGGATTTGCTTGAAGCGGAGCTGTTTGGCGCTGAGGCAGGCGCTTATACTGGCGCAACTAAGGCTCGTATTGGACGA
TTTGAAGCGGCCGATGGCGGTACATTGTTTTTAGATGAGATAGGTAATTTACCACTTTCTGGGCAGGTGAAGTTACTGCG
AGTATTGCAAACCGGTGAGTTTGAGCGACTCGGTAGCCATCAAACCCGCAGAGTCAATGTTCGAGTGGTAAGTGCCACTA
ATGCTGACTTGGCACAAGATATTCAAGCTGGGCGTTTTCGTGAGGATCTGTTCTACCGCCTCAATGTGATTGAGTTAGCC
TTGCCTGCACTTAATGAGCGTAAAGATGATGTATTGCCGTTGGTTGAACACTTTATTGGCACCGACTTTTCACTGTTGCG
CCAGACTCAGCAGGCGTTGGTGGCACACTCATGGCCAGGTAACGTGCGCGAGCTAGAAAATGCCTGTAAGCGAGCGGTGA
TATTAGCCAATTCCCCTGAGCTAACCGTCGAGGATTTTGGGCTCGTAATTCATACCAGTATGGCTGAGCCCGTCTCTACT
TCTGCGACTAATGATGCAACTAATCCAGTTACTAGCTCTGTCACAAACTTTGTTGCAGGGGCTTTAGTTGAAAAGTCAGC
AAGCCCTCAAACTCAAACGTACGCTTCAGCTAAGCATTCTTCGGCGGAGCCGATGAGTAACCCCGAAGCGAGTAATAATG
AGTCGAGTAATACTGAACAGAGTTGCACAGAGACGCCACAAGCTGAAAAAGCCAATATTGAAGCGGCGCTCGACCAGCAT
AGAGGCGTGATAGCTCGTGTCGCTAAGGCTCTGGGCTTAAGTCGACAAGCCTTATATCGGCGCATGGACAAGTATGGGAT
TGATAAGTAA

Upstream 100 bases:

>100_bases
TTGTTAGCCGAATGTGAGCTTTGCTTTAACTCTGTTCGGCTTTCAGTTAGTTTGATAGCCAGCCAAAAATAAAGAATGAA
GAATAAATACTAAAGAGTTT

Downstream 100 bases:

>100_bases
TTCATGAGCAATACAGTGGTTTCTCTTAGAGTTAAGCTTATTATCAGCGCAGTGCTCGCTTCAGCTACGGGCTTGTTGCT
GGCATTCACAGGCCTGTTAT

Product: Fis family two component sigma54 specific transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
METILIVDDNQAVCNALALMLELSGYQTLTCLSPDVALELIRLHDVALVIQDMNFTQDTTSGEEGRNLFYGFRQLQPELP
IILLTAWTQLELAVELVKEGAADYMGKPWDDAKLLNSISNLISLHRLSKKNAQLSRVESQRMVAIKDAELCGIVFNSGAM
QRCVDLALQIAKSDVSVLITGPNGAGKDKLADIIHANSPLRYKPFIKVNIGALPMDLLEAELFGAEAGAYTGATKARIGR
FEAADGGTLFLDEIGNLPLSGQVKLLRVLQTGEFERLGSHQTRRVNVRVVSATNADLAQDIQAGRFREDLFYRLNVIELA
LPALNERKDDVLPLVEHFIGTDFSLLRQTQQALVAHSWPGNVRELENACKRAVILANSPELTVEDFGLVIHTSMAEPVST
SATNDATNPVTSSVTNFVAGALVEKSASPQTQTYASAKHSSAEPMSNPEASNNESSNTEQSCTETPQAEKANIEAALDQH
RGVIARVAKALGLSRQALYRRMDKYGIDK

Sequences:

>Translated_509_residues
METILIVDDNQAVCNALALMLELSGYQTLTCLSPDVALELIRLHDVALVIQDMNFTQDTTSGEEGRNLFYGFRQLQPELP
IILLTAWTQLELAVELVKEGAADYMGKPWDDAKLLNSISNLISLHRLSKKNAQLSRVESQRMVAIKDAELCGIVFNSGAM
QRCVDLALQIAKSDVSVLITGPNGAGKDKLADIIHANSPLRYKPFIKVNIGALPMDLLEAELFGAEAGAYTGATKARIGR
FEAADGGTLFLDEIGNLPLSGQVKLLRVLQTGEFERLGSHQTRRVNVRVVSATNADLAQDIQAGRFREDLFYRLNVIELA
LPALNERKDDVLPLVEHFIGTDFSLLRQTQQALVAHSWPGNVRELENACKRAVILANSPELTVEDFGLVIHTSMAEPVST
SATNDATNPVTSSVTNFVAGALVEKSASPQTQTYASAKHSSAEPMSNPEASNNESSNTEQSCTETPQAEKANIEAALDQH
RGVIARVAKALGLSRQALYRRMDKYGIDK
>Mature_509_residues
METILIVDDNQAVCNALALMLELSGYQTLTCLSPDVALELIRLHDVALVIQDMNFTQDTTSGEEGRNLFYGFRQLQPELP
IILLTAWTQLELAVELVKEGAADYMGKPWDDAKLLNSISNLISLHRLSKKNAQLSRVESQRMVAIKDAELCGIVFNSGAM
QRCVDLALQIAKSDVSVLITGPNGAGKDKLADIIHANSPLRYKPFIKVNIGALPMDLLEAELFGAEAGAYTGATKARIGR
FEAADGGTLFLDEIGNLPLSGQVKLLRVLQTGEFERLGSHQTRRVNVRVVSATNADLAQDIQAGRFREDLFYRLNVIELA
LPALNERKDDVLPLVEHFIGTDFSLLRQTQQALVAHSWPGNVRELENACKRAVILANSPELTVEDFGLVIHTSMAEPVST
SATNDATNPVTSSVTNFVAGALVEKSASPQTQTYASAKHSSAEPMSNPEASNNESSNTEQSCTETPQAEKANIEAALDQH
RGVIARVAKALGLSRQALYRRMDKYGIDK

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=407, Percent_Identity=38.0835380835381, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1788550, Length=515, Percent_Identity=36.3106796116505, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1790437, Length=438, Percent_Identity=36.986301369863, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1788905, Length=394, Percent_Identity=39.3401015228426, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI87082117, Length=243, Percent_Identity=49.3827160493827, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI87082152, Length=261, Percent_Identity=44.0613026819923, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789087, Length=252, Percent_Identity=44.8412698412698, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1786524, Length=271, Percent_Identity=43.1734317343173, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789233, Length=356, Percent_Identity=35.9550561797753, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI87081872, Length=226, Percent_Identity=43.3628318584071, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1787583, Length=334, Percent_Identity=34.4311377245509, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1789828, Length=275, Percent_Identity=32, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081858, Length=355, Percent_Identity=27.3239436619718, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55482; Mature: 55482

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METILIVDDNQAVCNALALMLELSGYQTLTCLSPDVALELIRLHDVALVIQDMNFTQDTT
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHEEEEEECCCCCCCCC
SGEEGRNLFYGFRQLQPELPIILLTAWTQLELAVELVKEGAADYMGKPWDDAKLLNSISN
CCCCCCHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LISLHRLSKKNAQLSRVESQRMVAIKDAELCGIVFNSGAMQRCVDLALQIAKSDVSVLIT
HHHHHHHHHCCHHHHHHHHCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEE
GPNGAGKDKLADIIHANSPLRYKPFIKVNIGALPMDLLEAELFGAEAGAYTGATKARIGR
CCCCCCHHHHHHHHHCCCCCEECCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHCCC
FEAADGGTLFLDEIGNLPLSGQVKLLRVLQTGEFERLGSHQTRRVNVRVVSATNADLAQD
EECCCCCEEEHHHHCCCCCCCCHHHHHHHHCCCHHHHCCCCCEEEEEEEEEECCCHHHHH
IQAGRFREDLFYRLNVIELALPALNERKDDVLPLVEHFIGTDFSLLRQTQQALVAHSWPG
HHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
NVRELENACKRAVILANSPELTVEDFGLVIHTSMAEPVSTSATNDATNPVTSSVTNFVAG
CHHHHHHHHHHEEEEECCCCCCHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
ALVEKSASPQTQTYASAKHSSAEPMSNPEASNNESSNTEQSCTETPQAEKANIEAALDQH
HHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHH
RGVIARVAKALGLSRQALYRRMDKYGIDK
CCHHHHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
METILIVDDNQAVCNALALMLELSGYQTLTCLSPDVALELIRLHDVALVIQDMNFTQDTT
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHEEEEEECCCCCCCCC
SGEEGRNLFYGFRQLQPELPIILLTAWTQLELAVELVKEGAADYMGKPWDDAKLLNSISN
CCCCCCHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LISLHRLSKKNAQLSRVESQRMVAIKDAELCGIVFNSGAMQRCVDLALQIAKSDVSVLIT
HHHHHHHHHCCHHHHHHHHCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEE
GPNGAGKDKLADIIHANSPLRYKPFIKVNIGALPMDLLEAELFGAEAGAYTGATKARIGR
CCCCCCHHHHHHHHHCCCCCEECCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHCCC
FEAADGGTLFLDEIGNLPLSGQVKLLRVLQTGEFERLGSHQTRRVNVRVVSATNADLAQD
EECCCCCEEEHHHHCCCCCCCCHHHHHHHHCCCHHHHCCCCCEEEEEEEEEECCCHHHHH
IQAGRFREDLFYRLNVIELALPALNERKDDVLPLVEHFIGTDFSLLRQTQQALVAHSWPG
HHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
NVRELENACKRAVILANSPELTVEDFGLVIHTSMAEPVSTSATNDATNPVTSSVTNFVAG
CHHHHHHHHHHEEEEECCCCCCHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
ALVEKSASPQTQTYASAKHSSAEPMSNPEASNNESSNTEQSCTETPQAEKANIEAALDQH
HHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHH
RGVIARVAKALGLSRQALYRRMDKYGIDK
CCHHHHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]