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The map label for this gene is ydaM [H]

Identifier: 157963083

GI number: 157963083

Start: 4008015

End: 4009160

Strand: Reverse

Name: ydaM [H]

Synonym: Spea_3267

Alternate gene names: 157963083

Gene position: 4009160-4008015 (Counterclockwise)

Preceding gene: 157963084

Following gene: 157963082

Centisome position: 77.48

GC content: 46.25

Gene sequence:

>1146_bases
ATGATCGCCATAGGTCTAGCTTTGACTTTGTTCCAGTTTAATCAAGGCCAGTTACAAGTTTGGCAAGGGGTGATTGAGCA
ACTTCCATACTGGCTACTCCCTGCTTGCATTGCGATAGCGCTGCAATTTAACCGTAGTCGACTAGCTTATCTTGCAGCAT
TCATGCTGGCTTACTTTGTTTGTACGACAAACGATTGGTTGGGCACGATAGAGCAAGAGTATGCCGAGCAGATCTTGCTT
GGCGGCACGTTCATTATGACTTGGTTTGCTTTCATTAAAGATAGGGGGCTCTTATCACCCCATAGCTTAGTCAGGCTAAT
GGGCATAATAGTTAGCTTCGCGGCGGCTTTTGGCTGGCTATGGCTGAACACTGAATTTAAGCCGCTATTGGATCAATACC
TGCCTGAACTGTTTAATGTGGATGTGAGAACATTAGCGCCTCTTTATCTGTGCATCTTTATCGTAGCGATACGCGCAGCT
TGGCAGGCTAACTTGGTTAACACTTCTATATTTATCAGCTTAGCCTTATGGACTGCACAATTTATTCGCCCAGAGACACT
GCCGCTGGCGGTATCGATGACGGCATTAGCTAGCATTTACATTTTGACGGTTCTGACTGACTCCTATTTTTTGGCCTATC
GTGATGAGTTAACGGGCTTAGCTTCACGCCGTGCCTTGTTTAACTTAGTGCTATCTTTAGGCCGTAAATACAGTGTGGCT
ATGCTCGATATCGATCATTTTAAGAAATTCAACGACACCTATGGTCATGATGTTGGTGATCAGGTGCTAAAGCTAGTTGC
CAGTAAAATTGCAGGGGTTCGCGGTGGCGGAAGGGCCTTTCGCTACGGGGGGGAAGAGTTCACCATCATCTTTCCTCGTA
AAGACTCATCATCGACGTTGGATTATCTCGAAGAGGTGCGTGAGTCGATAGAGGAATACGACATCGTGCTGCGAGATGAG
AAACGTAAGAGTCAGTCAAAAGCCACCCGGGGCAAGACACAAGGTAAAGCTAAGACAGTGAGTGTCACCATCTCTATTGG
TGTTGCAGAGCACCAAAACGGTGAAACTTTTGAGCAAACCATGAAGCGGTCCGATCAGGCACTATATCGAGCCAAGAAAA
AAGGGCGTAATCAGGTTTGTGTTTAA

Upstream 100 bases:

>100_bases
GCTAATTGCTCTGAATTTCAGAGCTAGGCATTTAATTAGATTCATATTGAGGTTTGTTTTGTCTGCTATTTATCATAGTT
GCCGTTATTTTTTTATTCCT

Downstream 100 bases:

>100_bases
CGTCGTATTGTTTATCAGCCTTATTGTGATTGTTATAGGGAAATATTAATTATTTATTTGAACGTAGTTTTGAGCGGTCA
TTATAAGTGTTTAGTGCTGT

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MIAIGLALTLFQFNQGQLQVWQGVIEQLPYWLLPACIAIALQFNRSRLAYLAAFMLAYFVCTTNDWLGTIEQEYAEQILL
GGTFIMTWFAFIKDRGLLSPHSLVRLMGIIVSFAAAFGWLWLNTEFKPLLDQYLPELFNVDVRTLAPLYLCIFIVAIRAA
WQANLVNTSIFISLALWTAQFIRPETLPLAVSMTALASIYILTVLTDSYFLAYRDELTGLASRRALFNLVLSLGRKYSVA
MLDIDHFKKFNDTYGHDVGDQVLKLVASKIAGVRGGGRAFRYGGEEFTIIFPRKDSSSTLDYLEEVRESIEEYDIVLRDE
KRKSQSKATRGKTQGKAKTVSVTISIGVAEHQNGETFEQTMKRSDQALYRAKKKGRNQVCV

Sequences:

>Translated_381_residues
MIAIGLALTLFQFNQGQLQVWQGVIEQLPYWLLPACIAIALQFNRSRLAYLAAFMLAYFVCTTNDWLGTIEQEYAEQILL
GGTFIMTWFAFIKDRGLLSPHSLVRLMGIIVSFAAAFGWLWLNTEFKPLLDQYLPELFNVDVRTLAPLYLCIFIVAIRAA
WQANLVNTSIFISLALWTAQFIRPETLPLAVSMTALASIYILTVLTDSYFLAYRDELTGLASRRALFNLVLSLGRKYSVA
MLDIDHFKKFNDTYGHDVGDQVLKLVASKIAGVRGGGRAFRYGGEEFTIIFPRKDSSSTLDYLEEVRESIEEYDIVLRDE
KRKSQSKATRGKTQGKAKTVSVTISIGVAEHQNGETFEQTMKRSDQALYRAKKKGRNQVCV
>Mature_381_residues
MIAIGLALTLFQFNQGQLQVWQGVIEQLPYWLLPACIAIALQFNRSRLAYLAAFMLAYFVCTTNDWLGTIEQEYAEQILL
GGTFIMTWFAFIKDRGLLSPHSLVRLMGIIVSFAAAFGWLWLNTEFKPLLDQYLPELFNVDVRTLAPLYLCIFIVAIRAA
WQANLVNTSIFISLALWTAQFIRPETLPLAVSMTALASIYILTVLTDSYFLAYRDELTGLASRRALFNLVLSLGRKYSVA
MLDIDHFKKFNDTYGHDVGDQVLKLVASKIAGVRGGGRAFRYGGEEFTIIFPRKDSSSTLDYLEEVRESIEEYDIVLRDE
KRKSQSKATRGKTQGKAKTVSVTISIGVAEHQNGETFEQTMKRSDQALYRAKKKGRNQVCV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=34.6590909090909, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787262, Length=189, Percent_Identity=34.3915343915344, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693134, Length=173, Percent_Identity=32.9479768786127, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082007, Length=221, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787802, Length=174, Percent_Identity=32.7586206896552, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786584, Length=180, Percent_Identity=28.8888888888889, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787816, Length=187, Percent_Identity=31.5508021390374, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787056, Length=176, Percent_Identity=28.4090909090909, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788381, Length=180, Percent_Identity=26.1111111111111, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787541, Length=180, Percent_Identity=30, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=29.608938547486, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI87081977, Length=107, Percent_Identity=32.7102803738318, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656 [H]

Pfam domain/function: PF00990 GGDEF; PF08448 PAS_4 [H]

EC number: NA

Molecular weight: Translated: 43072; Mature: 43072

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIGLALTLFQFNQGQLQVWQGVIEQLPYWLLPACIAIALQFNRSRLAYLAAFMLAYFV
CEEEHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
CTTNDWLGTIEQEYAEQILLGGTFIMTWFAFIKDRGLLSPHSLVRLMGIIVSFAAAFGWL
HCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
WLNTEFKPLLDQYLPELFNVDVRTLAPLYLCIFIVAIRAAWQANLVNTSIFISLALWTAQ
EECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIRPETLPLAVSMTALASIYILTVLTDSYFLAYRDELTGLASRRALFNLVLSLGRKYSVA
HCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
MLDIDHFKKFNDTYGHDVGDQVLKLVASKIAGVRGGGRAFRYGGEEFTIIFPRKDSSSTL
EEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCCCHHH
DYLEEVRESIEEYDIVLRDEKRKSQSKATRGKTQGKAKTVSVTISIGVAEHQNGETFEQT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECCCCCHHHHH
MKRSDQALYRAKKKGRNQVCV
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIAIGLALTLFQFNQGQLQVWQGVIEQLPYWLLPACIAIALQFNRSRLAYLAAFMLAYFV
CEEEHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
CTTNDWLGTIEQEYAEQILLGGTFIMTWFAFIKDRGLLSPHSLVRLMGIIVSFAAAFGWL
HCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
WLNTEFKPLLDQYLPELFNVDVRTLAPLYLCIFIVAIRAAWQANLVNTSIFISLALWTAQ
EECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIRPETLPLAVSMTALASIYILTVLTDSYFLAYRDELTGLASRRALFNLVLSLGRKYSVA
HCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
MLDIDHFKKFNDTYGHDVGDQVLKLVASKIAGVRGGGRAFRYGGEEFTIIFPRKDSSSTL
EEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCCCHHH
DYLEEVRESIEEYDIVLRDEKRKSQSKATRGKTQGKAKTVSVTISIGVAEHQNGETFEQT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECCCCCHHHHH
MKRSDQALYRAKKKGRNQVCV
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]