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The map label for this gene is 157963049

Identifier: 157963049

GI number: 157963049

Start: 3962085

End: 3963554

Strand: Reverse

Name: 157963049

Synonym: Spea_3232

Alternate gene names: NA

Gene position: 3963554-3962085 (Counterclockwise)

Preceding gene: 157963050

Following gene: 157963048

Centisome position: 76.6

GC content: 48.5

Gene sequence:

>1470_bases
ATGCAGATGACTCTGTCTTTTTTAGGTGCGACTCAGGAAGTGACAGGCTCTTGTCACTTACTCACGATTGATGGACGACA
AGTGCTGCTCGATTGCGGCTTGATCCAAGGGAGTAAGGCCGATGTGTTGCGTAATCACGAGCCTTTTGCCTTCACTCCAT
CACAGATCCACGCAGTAGTTTTAAGTCATGCTCATATCGATCACTCAGGTCGGCTCCCTCTGTTAGTTAAATCTGGGTTT
ACAGGCCCTATCTATAGCCACAAGGCCACGGTAGAGCTGTGTGGCGTCATGCTAAAAGATGCCGCCATGTTGCAGGCAAG
AGATACTGAAAGACTCAACAAGAAAAGGGCAAAGAATGATCTGCCTTTGCTTGACCCCTTATTTGATGAAGCTGATGTTG
AGCGAGTGATGCTACAGTTTGTGGCACTAGATTATGGCGAGAATAAAGAGGTGGCCCCTCATCTTAAAGTATGTCTGTCG
GATGCGGGGCATATTTTGGGCTCTGCTGTGGTTGAGCTTTGGTTAGGAGAAGGTTCAGAAACTAAGAAACTGGTATTTAG
TGGCGATCTAGGCCGCGAGGGGATGCCGATTCTTGATGATCCAACATTCATCGATAGCGCAGATCTGGTGTTGATGGAAA
GTACTTATGGTGACAGGCTGCATCGCAGTTGGGATGATACCCTTGAGGAGTTGAGAGCGATATTTGCCCGCACCATTGAA
CAGAGTCGTGGCAATATTCTGTTGCCAGCATTTTCGGTTGGCCGAGCGCAGGAGCTGCTCTACCTGTTTCATCTCTACGC
TAAGGAGTGGGATCTCTCTCGCTGGCGGATCTGCCTCGATAGCCCTATGGCAATCAAAGCGACACAGATCTATGTCGATA
ATTATGGCCTTATGGATGAGGATTTTAAACGCTTCACCCATATGTCTCCCGGCAAGCACCCTCTACTGTCCAATGTGGAC
TTTATCGACACCACTGAGGAGTCGATGGAGCTCAACCATATCCACCAAGGACTAATTATTATCGCAGGTAGCGGCATGTG
TAACGGTGGTCGAATCCGTTGTCACTTAGAGCACAACTTAGCTAATAGCTTCTCTGACGTGATTATTTGTGGCTATCAAG
CGCATGGTACTCCTGGGCGACTCTTGGTGGATGGCGCAGAGAGTCTGACTATCAGTGGGCAAAGCATTAATGTGGCGGCA
AGGTTACATACGGTCGGAGGACTCTCGGCTCATGCGGATCAGGCGGAGTTATTGTGCTGGTATCGACACTTTAATGATTC
TCCTCCCATCGTATTAGTACACGGAGAGGTAGAGGCGCAAAAGGTGTTACTAGCTCAACTTAATGCAAATAGCAGCTTAG
CTCCTAGACAAAGTGCGATAGCAGAGCAAGGTAATTGCCTGGATCTTAGTCGGCTGCCTAAATTTGTTTGGTTACCGAGC
GCTCTTGGCAATGGGAAGCAAGATGAGTGA

Upstream 100 bases:

>100_bases
AAAAGCCATTGAGGGTTTATCTTTTAAGCATAAGCACCACAAATGCCCCCTGTGTTGAACTCGCCTAAAGGGAATAAAGG
TGCCTTTGAGGAAGAATGCC

Downstream 100 bases:

>100_bases
GCCTCAAGATGTAAAACCTCTGTGATGGATGATACTATCTCGAAACATTAATGTCATATTCAGAGTGTCTAGCTTGTCGT
TTAAGTGGCTGTTATTTATT

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MQMTLSFLGATQEVTGSCHLLTIDGRQVLLDCGLIQGSKADVLRNHEPFAFTPSQIHAVVLSHAHIDHSGRLPLLVKSGF
TGPIYSHKATVELCGVMLKDAAMLQARDTERLNKKRAKNDLPLLDPLFDEADVERVMLQFVALDYGENKEVAPHLKVCLS
DAGHILGSAVVELWLGEGSETKKLVFSGDLGREGMPILDDPTFIDSADLVLMESTYGDRLHRSWDDTLEELRAIFARTIE
QSRGNILLPAFSVGRAQELLYLFHLYAKEWDLSRWRICLDSPMAIKATQIYVDNYGLMDEDFKRFTHMSPGKHPLLSNVD
FIDTTEESMELNHIHQGLIIIAGSGMCNGGRIRCHLEHNLANSFSDVIICGYQAHGTPGRLLVDGAESLTISGQSINVAA
RLHTVGGLSAHADQAELLCWYRHFNDSPPIVLVHGEVEAQKVLLAQLNANSSLAPRQSAIAEQGNCLDLSRLPKFVWLPS
ALGNGKQDE

Sequences:

>Translated_489_residues
MQMTLSFLGATQEVTGSCHLLTIDGRQVLLDCGLIQGSKADVLRNHEPFAFTPSQIHAVVLSHAHIDHSGRLPLLVKSGF
TGPIYSHKATVELCGVMLKDAAMLQARDTERLNKKRAKNDLPLLDPLFDEADVERVMLQFVALDYGENKEVAPHLKVCLS
DAGHILGSAVVELWLGEGSETKKLVFSGDLGREGMPILDDPTFIDSADLVLMESTYGDRLHRSWDDTLEELRAIFARTIE
QSRGNILLPAFSVGRAQELLYLFHLYAKEWDLSRWRICLDSPMAIKATQIYVDNYGLMDEDFKRFTHMSPGKHPLLSNVD
FIDTTEESMELNHIHQGLIIIAGSGMCNGGRIRCHLEHNLANSFSDVIICGYQAHGTPGRLLVDGAESLTISGQSINVAA
RLHTVGGLSAHADQAELLCWYRHFNDSPPIVLVHGEVEAQKVLLAQLNANSSLAPRQSAIAEQGNCLDLSRLPKFVWLPS
ALGNGKQDE
>Mature_489_residues
MQMTLSFLGATQEVTGSCHLLTIDGRQVLLDCGLIQGSKADVLRNHEPFAFTPSQIHAVVLSHAHIDHSGRLPLLVKSGF
TGPIYSHKATVELCGVMLKDAAMLQARDTERLNKKRAKNDLPLLDPLFDEADVERVMLQFVALDYGENKEVAPHLKVCLS
DAGHILGSAVVELWLGEGSETKKLVFSGDLGREGMPILDDPTFIDSADLVLMESTYGDRLHRSWDDTLEELRAIFARTIE
QSRGNILLPAFSVGRAQELLYLFHLYAKEWDLSRWRICLDSPMAIKATQIYVDNYGLMDEDFKRFTHMSPGKHPLLSNVD
FIDTTEESMELNHIHQGLIIIAGSGMCNGGRIRCHLEHNLANSFSDVIICGYQAHGTPGRLLVDGAESLTISGQSINVAA
RLHTVGGLSAHADQAELLCWYRHFNDSPPIVLVHGEVEAQKVLLAQLNANSSLAPRQSAIAEQGNCLDLSRLPKFVWLPS
ALGNGKQDE

Specific function: Has endoribonuclease activity towards 23S and 16S rRNA (in vitro) [H]

COG id: COG1236

COG function: function code J; Predicted exonuclease of the beta-lactamase fold involved in RNA processing

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family [H]

Homologues:

Organism=Homo sapiens, GI7706427, Length=467, Percent_Identity=28.2655246252677, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI33300633, Length=459, Percent_Identity=25.0544662309368, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564696, Length=495, Percent_Identity=24.6464646464646, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566029, Length=447, Percent_Identity=26.8456375838926, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323307, Length=493, Percent_Identity=24.3407707910751, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24648013, Length=437, Percent_Identity=28.3752860411899, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21358523, Length=476, Percent_Identity=25.2100840336134, Blast_Score=122, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022712
- InterPro:   IPR001279
- InterPro:   IPR011108 [H]

Pfam domain/function: PF10996 Beta-Casp; PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 53955; Mature: 53955

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMTLSFLGATQEVTGSCHLLTIDGRQVLLDCGLIQGSKADVLRNHEPFAFTPSQIHAVV
CEEEEEHHCCCHHCCCCEEEEEECCCEEEEEECCCCCCCHHHHCCCCCCEECHHHHHEEE
LSHAHIDHSGRLPLLVKSGFTGPIYSHKATVELCGVMLKDAAMLQARDTERLNKKRAKND
HHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPLLDPLFDEADVERVMLQFVALDYGENKEVAPHLKVCLSDAGHILGSAVVELWLGEGSE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TKKLVFSGDLGREGMPILDDPTFIDSADLVLMESTYGDRLHRSWDDTLEELRAIFARTIE
CCEEEEECCCCCCCCCCCCCCCEECCCCEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHH
QSRGNILLPAFSVGRAQELLYLFHLYAKEWDLSRWRICLDSPMAIKATQIYVDNYGLMDE
HCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEECCCCCCH
DFKRFTHMSPGKHPLLSNVDFIDTTEESMELNHIHQGLIIIAGSGMCNGGRIRCHLEHNL
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCC
ANSFSDVIICGYQAHGTPGRLLVDGAESLTISGQSINVAARLHTVGGLSAHADQAELLCW
CCCCCCEEEEEEECCCCCCEEEEECCCEEEECCCEEEEEEEEEECCCCCCCCCCHHHEEE
YRHFNDSPPIVLVHGEVEAQKVLLAQLNANSSLAPRQSAIAEQGNCLDLSRLPKFVWLPS
EECCCCCCCEEEEECCCCHHHEEEEEECCCCCCCCHHHHHHCCCCEEEHHHCCCEEEECH
ALGNGKQDE
HHCCCCCCC
>Mature Secondary Structure
MQMTLSFLGATQEVTGSCHLLTIDGRQVLLDCGLIQGSKADVLRNHEPFAFTPSQIHAVV
CEEEEEHHCCCHHCCCCEEEEEECCCEEEEEECCCCCCCHHHHCCCCCCEECHHHHHEEE
LSHAHIDHSGRLPLLVKSGFTGPIYSHKATVELCGVMLKDAAMLQARDTERLNKKRAKND
HHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPLLDPLFDEADVERVMLQFVALDYGENKEVAPHLKVCLSDAGHILGSAVVELWLGEGSE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TKKLVFSGDLGREGMPILDDPTFIDSADLVLMESTYGDRLHRSWDDTLEELRAIFARTIE
CCEEEEECCCCCCCCCCCCCCCEECCCCEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHH
QSRGNILLPAFSVGRAQELLYLFHLYAKEWDLSRWRICLDSPMAIKATQIYVDNYGLMDE
HCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEECCCCCCH
DFKRFTHMSPGKHPLLSNVDFIDTTEESMELNHIHQGLIIIAGSGMCNGGRIRCHLEHNL
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCC
ANSFSDVIICGYQAHGTPGRLLVDGAESLTISGQSINVAARLHTVGGLSAHADQAELLCW
CCCCCCEEEEEEECCCCCCEEEEECCCEEEECCCEEEEEEEEEECCCCCCCCCCHHHEEE
YRHFNDSPPIVLVHGEVEAQKVLLAQLNANSSLAPRQSAIAEQGNCLDLSRLPKFVWLPS
EECCCCCCCEEEEECCCCHHHEEEEEECCCCCCCCHHHHHHCCCCEEEHHHCCCEEEECH
ALGNGKQDE
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA