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The map label for this gene is hemK [H]

Identifier: 157962942

GI number: 157962942

Start: 3828059

End: 3828901

Strand: Reverse

Name: hemK [H]

Synonym: Spea_3125

Alternate gene names: 157962942

Gene position: 3828901-3828059 (Counterclockwise)

Preceding gene: 157962943

Following gene: 157962941

Centisome position: 73.99

GC content: 45.31

Gene sequence:

>843_bases
TTGCAATTAACTATCTCAGGGGCGCTTAAGTGGGCCTCTTCTCAGCTTGAAAGTATTTCAGACTCAGCATCACTGGATGC
TGAGGTCATGTTGCTTCATGTAATTAATAAACAGCGTAGCTACTTATATACTTGGCCTGATAGAAGCTTAGAACCCGAGC
AAGTTTTTGAGTTTAAACAGATGTTGGCCAAGCGTTTGCGTGGTAATCCAATCGCGCATATCGTTGGTGAAAGAGAGTTT
TGGTCACTGCCTTTTAGAGTGAATCCAACAACGCTGATCCCACGCCCCGATACAGAAATCTTGGTTGAAACAGCGCTGAA
TTTACCTTTAGCTGAAAGTGCTAAGGTACTCGATTTAGGCACTGGCACGGGTGCTATCGCCTTGTCATTGGCCCATGAGC
GTAGCGAATGGCAGGTTTGTGCGATCGATAAAGTGGATGATGCTGTGGCATTAGCCAAGGAAAATCGTACTAATTTGAAA
CTCGAACAGGTTGATGTGTTTCAAAGCGACTGGTTTGATGCTGTTGAGTGCTATGACTTTAATTTGATTGTGTCCAATCC
GCCTTATATCGATGAAGAGGATGAACACCTTTCTCAAGGCGATGTTAGATTTGAGCCAGCGAGTGCCTTAACTGCACCAC
AGAGGGGCTTTGCAGATCTGTTTTACATTGCAGAGTCGGCTCGTGATTACTTAGCACCAGGTGGCTATTTGCTACTCGAG
CACGGTTATCAGCAAGCTATCGAGTTAAGAGAGAAGTTAATTGAGCTGGGTTATGAAAACGTTGCCACGGTTCGAGACTT
TGGCAGTAATGATCGCTGCACATTAGGTCGTTGGCCTCGTTAG

Upstream 100 bases:

>100_bases
CACTGATCCAAGAAAACCAAGCGGATATGCTTGCAGCACTGGGTGAAGGCTAAGCCTTCTCTCACACTATCATTGTGCTA
CAGATGAATAAGGATATTCC

Downstream 100 bases:

>100_bases
AGGTTTATCGATTGATGAAGAAAAAGGCATTCTCGTGAATGCCTTTTTTTATGCCCCGATTACGTCAGCTGTTTCACCAT
AAAATATTTACAGCCAGTTG

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.VchAHemK2P [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MQLTISGALKWASSQLESISDSASLDAEVMLLHVINKQRSYLYTWPDRSLEPEQVFEFKQMLAKRLRGNPIAHIVGEREF
WSLPFRVNPTTLIPRPDTEILVETALNLPLAESAKVLDLGTGTGAIALSLAHERSEWQVCAIDKVDDAVALAKENRTNLK
LEQVDVFQSDWFDAVECYDFNLIVSNPPYIDEEDEHLSQGDVRFEPASALTAPQRGFADLFYIAESARDYLAPGGYLLLE
HGYQQAIELREKLIELGYENVATVRDFGSNDRCTLGRWPR

Sequences:

>Translated_280_residues
MQLTISGALKWASSQLESISDSASLDAEVMLLHVINKQRSYLYTWPDRSLEPEQVFEFKQMLAKRLRGNPIAHIVGEREF
WSLPFRVNPTTLIPRPDTEILVETALNLPLAESAKVLDLGTGTGAIALSLAHERSEWQVCAIDKVDDAVALAKENRTNLK
LEQVDVFQSDWFDAVECYDFNLIVSNPPYIDEEDEHLSQGDVRFEPASALTAPQRGFADLFYIAESARDYLAPGGYLLLE
HGYQQAIELREKLIELGYENVATVRDFGSNDRCTLGRWPR
>Mature_280_residues
MQLTISGALKWASSQLESISDSASLDAEVMLLHVINKQRSYLYTWPDRSLEPEQVFEFKQMLAKRLRGNPIAHIVGEREF
WSLPFRVNPTTLIPRPDTEILVETALNLPLAESAKVLDLGTGTGAIALSLAHERSEWQVCAIDKVDDAVALAKENRTNLK
LEQVDVFQSDWFDAVECYDFNLIVSNPPYIDEEDEHLSQGDVRFEPASALTAPQRGFADLFYIAESARDYLAPGGYLLLE
HGYQQAIELREKLIELGYENVATVRDFGSNDRCTLGRWPR

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=235, Percent_Identity=30.6382978723404, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787463, Length=270, Percent_Identity=50.3703703703704, Blast_Score=278, Evalue=2e-76,
Organism=Escherichia coli, GI87082085, Length=175, Percent_Identity=32.5714285714286, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24582226, Length=246, Percent_Identity=28.4552845528455, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31618; Mature: 31618

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTISGALKWASSQLESISDSASLDAEVMLLHVINKQRSYLYTWPDRSLEPEQVFEFKQ
CEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHEEHHCCCCCEEEECCCCCCCHHHHHHHHH
MLAKRLRGNPIAHIVGEREFWSLPFRVNPTTLIPRPDTEILVETALNLPLAESAKVLDLG
HHHHHHCCCCEEEEECCCCEEECCEEECCCEECCCCCHHHHHHHHHCCCCCCCCEEEEEC
TGTGAIALSLAHERSEWQVCAIDKVDDAVALAKENRTNLKLEQVDVFQSDWFDAVECYDF
CCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCEEHHHHHHHHCCCCCEEEEEE
NLIVSNPPYIDEEDEHLSQGDVRFEPASALTAPQRGFADLFYIAESARDYLAPGGYLLLE
EEEECCCCCCCCCHHHHCCCCEEECCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
HGYQQAIELREKLIELGYENVATVRDFGSNDRCTLGRWPR
CCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQLTISGALKWASSQLESISDSASLDAEVMLLHVINKQRSYLYTWPDRSLEPEQVFEFKQ
CEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHEEHHCCCCCEEEECCCCCCCHHHHHHHHH
MLAKRLRGNPIAHIVGEREFWSLPFRVNPTTLIPRPDTEILVETALNLPLAESAKVLDLG
HHHHHHCCCCEEEEECCCCEEECCEEECCCEECCCCCHHHHHHHHHCCCCCCCCEEEEEC
TGTGAIALSLAHERSEWQVCAIDKVDDAVALAKENRTNLKLEQVDVFQSDWFDAVECYDF
CCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCEEHHHHHHHHCCCCCEEEEEE
NLIVSNPPYIDEEDEHLSQGDVRFEPASALTAPQRGFADLFYIAESARDYLAPGGYLLLE
EEEECCCCCCCCCHHHHCCCCEEECCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
HGYQQAIELREKLIELGYENVATVRDFGSNDRCTLGRWPR
CCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]