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The map label for this gene is prfA [H]

Identifier: 157962943

GI number: 157962943

Start: 3828949

End: 3830034

Strand: Reverse

Name: prfA [H]

Synonym: Spea_3126

Alternate gene names: 157962943

Gene position: 3830034-3828949 (Counterclockwise)

Preceding gene: 157962944

Following gene: 157962942

Centisome position: 74.02

GC content: 46.41

Gene sequence:

>1086_bases
ATGAAGGACAGTGTGATTCGTAAGCTTGAAGGCTTACTTGAACGTAATGAAGAAGTGTTGGCGTTACTGAGTGATGCAGG
CGTTATTGCCGATCAGGAACGTTTCCGCGCCTTGTCTAAAGAATATTCTCAACTAGAGGATGTAGTCAGTACCTTTAAGT
CTTTTCAGCAAGCCGAGGAAGATCTCGAGTCAGCTAAAGAGATGATGGAAGAGGATGACCCAGAGCTTAAAGAGATGGCT
CAGGAAGAATATAAAGTCGCTAAGTCGGCGATAGCAGCGCTTGAAGATGAGTTGCAGATCTTATTGCTTCCAAAAGATCC
GAATGACGACAATAACTGTTTTATCGAAATCCGCGCTGGCGCGGGTGGTGATGAAGCGGCTATTTTTGCGGGTGATTTGT
TCCGCATGTATAGCAGATACGCCGAAAGTAAGCGCTGGCAGATAGAAGTTATGAACACCAACGAAGGTGAGCATGGCGGC
TTTAAAGAAGTGATCGCTAAAATCAGTGGCGAAGGTGTTTACGGTAAGCTGAAATTTGAATCTGGCGGACACCGTGTACA
ACGTGTACCTGAGACCGAGTCTCAAGGCCGCGTACATACTTCAGCTTGTACTGTGATTGTACTGCCTGAAGTACCAGAAG
CAGAAGCGATTGAAATTAACAAGGCCGACTTGAAAGTGGATACTTTCCGCGCATCAGGTGCTGGTGGACAGCACGTTAAC
AAAACTGACTCAGCTATTCGTATTACCCATATTCCAACAGGTATCGTTGTAGAGTGTCAGGATCAGCGTTCACAGCATAA
AAACCGTGCCCAAGCGATGAGCGTGCTTGCCGCGCGTATCCAAGCTGTTGAAGACGAGAAGCGTCGTAGTGCTGAAGAAA
GTACACGCCGTAACTTGGTGTCATCAGGTGATCGTTCAGAACGTATTCGTACTTATAACTTCCCACAAGGTCGTGTAAGT
GAGCACAGAATTAACTTAACTCTGTACCGCTTAGGCGAATTTATGGAAGGTGACATCGATTGTGTTGTAGAACCACTGAT
CCAAGAAAACCAAGCGGATATGCTTGCAGCACTGGGTGAAGGCTAA

Upstream 100 bases:

>100_bases
ACACAAGCGCTTACCGCTGCGAGTCGACAAGGGGACTTGAATTCCCTTGGACAATTAAGAGCTTTGCTTGGACTAGATAA
AGACTAAGGTTAGCGTTTTA

Downstream 100 bases:

>100_bases
GCCTTCTCTCACACTATCATTGTGCTACAGATGAATAAGGATATTCCTTGCAATTAACTATCTCAGGGGCGCTTAAGTGG
GCCTCTTCTCAGCTTGAAAG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKDSVIRKLEGLLERNEEVLALLSDAGVIADQERFRALSKEYSQLEDVVSTFKSFQQAEEDLESAKEMMEEDDPELKEMA
QEEYKVAKSAIAALEDELQILLLPKDPNDDNNCFIEIRAGAGGDEAAIFAGDLFRMYSRYAESKRWQIEVMNTNEGEHGG
FKEVIAKISGEGVYGKLKFESGGHRVQRVPETESQGRVHTSACTVIVLPEVPEAEAIEINKADLKVDTFRASGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRNLVSSGDRSERIRTYNFPQGRVS
EHRINLTLYRLGEFMEGDIDCVVEPLIQENQADMLAALGEG

Sequences:

>Translated_361_residues
MKDSVIRKLEGLLERNEEVLALLSDAGVIADQERFRALSKEYSQLEDVVSTFKSFQQAEEDLESAKEMMEEDDPELKEMA
QEEYKVAKSAIAALEDELQILLLPKDPNDDNNCFIEIRAGAGGDEAAIFAGDLFRMYSRYAESKRWQIEVMNTNEGEHGG
FKEVIAKISGEGVYGKLKFESGGHRVQRVPETESQGRVHTSACTVIVLPEVPEAEAIEINKADLKVDTFRASGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRNLVSSGDRSERIRTYNFPQGRVS
EHRINLTLYRLGEFMEGDIDCVVEPLIQENQADMLAALGEG
>Mature_361_residues
MKDSVIRKLEGLLERNEEVLALLSDAGVIADQERFRALSKEYSQLEDVVSTFKSFQQAEEDLESAKEMMEEDDPELKEMA
QEEYKVAKSAIAALEDELQILLLPKDPNDDNNCFIEIRAGAGGDEAAIFAGDLFRMYSRYAESKRWQIEVMNTNEGEHGG
FKEVIAKISGEGVYGKLKFESGGHRVQRVPETESQGRVHTSACTVIVLPEVPEAEAIEINKADLKVDTFRASGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRNLVSSGDRSERIRTYNFPQGRVS
EHRINLTLYRLGEFMEGDIDCVVEPLIQENQADMLAALGEG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=43.7086092715232, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI34577120, Length=344, Percent_Identity=41.8604651162791, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=42.512077294686, Blast_Score=191, Evalue=9e-49,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=70.2777777777778, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=40.5325443786982, Blast_Score=229, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17542784, Length=268, Percent_Identity=38.8059701492537, Blast_Score=184, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=41.7721518987342, Blast_Score=249, Evalue=4e-67,
Organism=Drosophila melanogaster, GI19921226, Length=328, Percent_Identity=40.2439024390244, Blast_Score=222, Evalue=3e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40320; Mature: 40320

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSVIRKLEGLLERNEEVLALLSDAGVIADQERFRALSKEYSQLEDVVSTFKSFQQAEE
CCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLESAKEMMEEDDPELKEMAQEEYKVAKSAIAALEDELQILLLPKDPNDDNNCFIEIRAG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEC
AGGDEAAIFAGDLFRMYSRYAESKRWQIEVMNTNEGEHGGFKEVIAKISGEGVYGKLKFE
CCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEC
SGGHRVQRVPETESQGRVHTSACTVIVLPEVPEAEAIEINKADLKVDTFRASGAGGQHVN
CCCCCEEECCCCCCCCCEEECEEEEEEECCCCCCCEEEEECCCEEEEEEEECCCCCCCCC
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRNLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSGDRSERIRTYNFPQGRVSEHRINLTLYRLGEFMEGDIDCVVEPLIQENQADMLAALGE
HCCCCCCCCEECCCCCCCCCHHEEEEEHEEHHHHHCCCHHHHHHHHHCCCHHHHHHHCCC
G
C
>Mature Secondary Structure
MKDSVIRKLEGLLERNEEVLALLSDAGVIADQERFRALSKEYSQLEDVVSTFKSFQQAEE
CCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLESAKEMMEEDDPELKEMAQEEYKVAKSAIAALEDELQILLLPKDPNDDNNCFIEIRAG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEC
AGGDEAAIFAGDLFRMYSRYAESKRWQIEVMNTNEGEHGGFKEVIAKISGEGVYGKLKFE
CCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEC
SGGHRVQRVPETESQGRVHTSACTVIVLPEVPEAEAIEINKADLKVDTFRASGAGGQHVN
CCCCCEEECCCCCCCCCEEECEEEEEEECCCCCCCEEEEECCCEEEEEEEECCCCCCCCC
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRNLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSGDRSERIRTYNFPQGRVSEHRINLTLYRLGEFMEGDIDCVVEPLIQENQADMLAALGE
HCCCCCCCCEECCCCCCCCCHHEEEEEHEEHHHHHCCCHHHHHHHHHCCCHHHHHHHCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA