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The map label for this gene is apbE [H]

Identifier: 157962914

GI number: 157962914

Start: 3799429

End: 3800445

Strand: Reverse

Name: apbE [H]

Synonym: Spea_3097

Alternate gene names: 157962914

Gene position: 3800445-3799429 (Counterclockwise)

Preceding gene: 157962915

Following gene: 157962913

Centisome position: 73.44

GC content: 44.25

Gene sequence:

>1017_bases
ATGTTTAAGACCTTAGTAAACTGGCTGGCCCTTATCGGGTTAGCCTTTTTTATTTCAGCTTGTACTAAACAAGCTGAAGT
GATTTCACTGTCTGGCAACACTATGGGCACGACTTACCATATTAAGGTCGTTCCTAATGACAGAATGCCAGATGTTCAGT
TACTGCAAGCAGAGATCGATTTAGCCTTAGAGAATGTTAACGATCAGATGTCGACTTATCGTCCTAACTCTGAGTTATCT
AAGTTTAACCAGATGACTCGCCACGATAAGGTTGAAGTGTCGGCGGACACTGCCAAGGTAATTGCTGAAGGATTAGCATT
ATATAAAACCACTGATGGTGCCTTAGATATTACTCTGGGGCCGTTAGTCAATTTATGGGGCTTTGGCCCGGATAAACGAC
CGACTCAAATTCCATCTCAACAAGCTATCGATGCCGCTATGGATCGCACAGGTATTGAAGCGATAGAGGTCGATGGTAAT
AGGATCAGTAAACAAAACCCTGATATTTATGTCGACTTGTCTTCTATTGCAAAAGGCTTCGGTGTTGATAAAATAGCCGC
CTTATTAGATAAGTATCAGCCGACGGGCTATTTAGTTGAGATAGGTGGCGAGATCAGCCTACGTGGCACTAAAGCCGATG
GCACTCTATGGCGTATTGCGATTGAGCAACCTGGCGATGCTGGTGGGGCAATCCAACAAGTCATCTCACCCAAGAATATG
GCGATGGCAACATCCGGTGATTATCGTAACTATTACGAGGAAGACGGAAAACGTTTTTCACACCTGATCGACCCACGCAC
AGGCTACCCGATACACCATAGGTTAGCGTCGGTAACAGTACTGCATCAAGAATCTATGATTGCAGATGGCTACGCGACTG
CTATGATGGTGCTAGGTACCCAAGCGTCACTAGAACTTGCTAAACGTGAGCATCTAGCGATAATGCTTATTGAAAAGCAA
GATGATGGCTTTAAAGTTTTCTATAGTGAAGCTTTCAAACCTTTCGTTACTCAGTAG

Upstream 100 bases:

>100_bases
CTGTTATTAGCATGCTTAAAGATCTTGGTGTCGAAGATGAAAACATCCTGTTGGATGACTTCGGTGGCTAACCCTTCTCT
TATTGGAGATCTAGGTCAAT

Downstream 100 bases:

>100_bases
TTGGAGTCGAATATGAGCACATTTATTGCGGCTTTTGTCGTGTTATTAGGTTTCTTTTTCTTAATGTCGATAGGCTATTT
AGTCAAACGACAAGCCGTTG

Product: ApbE family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MFKTLVNWLALIGLAFFISACTKQAEVISLSGNTMGTTYHIKVVPNDRMPDVQLLQAEIDLALENVNDQMSTYRPNSELS
KFNQMTRHDKVEVSADTAKVIAEGLALYKTTDGALDITLGPLVNLWGFGPDKRPTQIPSQQAIDAAMDRTGIEAIEVDGN
RISKQNPDIYVDLSSIAKGFGVDKIAALLDKYQPTGYLVEIGGEISLRGTKADGTLWRIAIEQPGDAGGAIQQVISPKNM
AMATSGDYRNYYEEDGKRFSHLIDPRTGYPIHHRLASVTVLHQESMIADGYATAMMVLGTQASLELAKREHLAIMLIEKQ
DDGFKVFYSEAFKPFVTQ

Sequences:

>Translated_338_residues
MFKTLVNWLALIGLAFFISACTKQAEVISLSGNTMGTTYHIKVVPNDRMPDVQLLQAEIDLALENVNDQMSTYRPNSELS
KFNQMTRHDKVEVSADTAKVIAEGLALYKTTDGALDITLGPLVNLWGFGPDKRPTQIPSQQAIDAAMDRTGIEAIEVDGN
RISKQNPDIYVDLSSIAKGFGVDKIAALLDKYQPTGYLVEIGGEISLRGTKADGTLWRIAIEQPGDAGGAIQQVISPKNM
AMATSGDYRNYYEEDGKRFSHLIDPRTGYPIHHRLASVTVLHQESMIADGYATAMMVLGTQASLELAKREHLAIMLIEKQ
DDGFKVFYSEAFKPFVTQ
>Mature_338_residues
MFKTLVNWLALIGLAFFISACTKQAEVISLSGNTMGTTYHIKVVPNDRMPDVQLLQAEIDLALENVNDQMSTYRPNSELS
KFNQMTRHDKVEVSADTAKVIAEGLALYKTTDGALDITLGPLVNLWGFGPDKRPTQIPSQQAIDAAMDRTGIEAIEVDGN
RISKQNPDIYVDLSSIAKGFGVDKIAALLDKYQPTGYLVEIGGEISLRGTKADGTLWRIAIEQPGDAGGAIQQVISPKNM
AMATSGDYRNYYEEDGKRFSHLIDPRTGYPIHHRLASVTVLHQESMIADGYATAMMVLGTQASLELAKREHLAIMLIEKQ
DDGFKVFYSEAFKPFVTQ

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=340, Percent_Identity=40.2941176470588, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37207; Mature: 37207

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKTLVNWLALIGLAFFISACTKQAEVISLSGNTMGTTYHIKVVPNDRMPDVQLLQAEID
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHH
LALENVNDQMSTYRPNSELSKFNQMTRHDKVEVSADTAKVIAEGLALYKTTDGALDITLG
HHHHCCCHHHHHCCCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHHEEEECCCEEEEEHH
PLVNLWGFGPDKRPTQIPSQQAIDAAMDRTGIEAIEVDGNRISKQNPDIYVDLSSIAKGF
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEHHHHHCCC
GVDKIAALLDKYQPTGYLVEIGGEISLRGTKADGTLWRIAIEQPGDAGGAIQQVISPKNM
CHHHHHHHHHCCCCCCEEEEECCEEEEEEECCCCEEEEEEEECCCCCCHHHHHHCCCCCE
AMATSGDYRNYYEEDGKRFSHLIDPRTGYPIHHRLASVTVLHQESMIADGYATAMMVLGT
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEEEEHHHHHHCHHHHHHHHHCC
QASLELAKREHLAIMLIEKQDDGFKVFYSEAFKPFVTQ
HHHHHHHHCCCEEEEEEEECCCCEEEEEHHHCCHHCCC
>Mature Secondary Structure
MFKTLVNWLALIGLAFFISACTKQAEVISLSGNTMGTTYHIKVVPNDRMPDVQLLQAEID
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHH
LALENVNDQMSTYRPNSELSKFNQMTRHDKVEVSADTAKVIAEGLALYKTTDGALDITLG
HHHHCCCHHHHHCCCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHHEEEECCCEEEEEHH
PLVNLWGFGPDKRPTQIPSQQAIDAAMDRTGIEAIEVDGNRISKQNPDIYVDLSSIAKGF
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEHHHHHCCC
GVDKIAALLDKYQPTGYLVEIGGEISLRGTKADGTLWRIAIEQPGDAGGAIQQVISPKNM
CHHHHHHHHHCCCCCCEEEEECCEEEEEEECCCCEEEEEEEECCCCCCHHHHHHCCCCCE
AMATSGDYRNYYEEDGKRFSHLIDPRTGYPIHHRLASVTVLHQESMIADGYATAMMVLGT
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEEEEHHHHHHCHHHHHHHHHCC
QASLELAKREHLAIMLIEKQDDGFKVFYSEAFKPFVTQ
HHHHHHHHCCCEEEEEEEECCCCEEEEEHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]