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The map label for this gene is rbfA [H]

Identifier: 157962877

GI number: 157962877

Start: 3752630

End: 3753058

Strand: Reverse

Name: rbfA [H]

Synonym: Spea_3059

Alternate gene names: 157962877

Gene position: 3753058-3752630 (Counterclockwise)

Preceding gene: 157962878

Following gene: 157962876

Centisome position: 72.53

GC content: 44.76

Gene sequence:

>429_bases
ATGGCAAAAGAATTTAGTCGTACACGCCGTATCGCACAGCAGCTTCAACAAGAGCTTGCACAGGTGCTACAACGTGATAT
GAAAGATCCACGCATTGGGTTTGTGACGGTTAATGATGTCGATGTTTCTCGTGATTTAAGCTATGCAAAAGTTTATGTTA
CTTTCTTCGAAGAAGACGAAAAATTAGTCGAACAAAAAGTGGCTGCATTGGACGCTGCTGCGGGTTATATCCGCTCGTTA
GTTGCTGGTCGTATGAAGTTACGCGTTATGCCTGAACTTCGCTTTATCTACGATAGCTCGTTAGTTGAAGGTATGCGTAT
GTCTAACTTAGTTAGCCGCGTGATCAGTAACGACGAAGCTAAGCAGAAGCAACACGGCGTAGAAACCGACGCTGAACAAG
GCGACACTAAGGAAGAGGGCGATAAGTAA

Upstream 100 bases:

>100_bases
CTTCGAGACCGTTGAGATAGCACGTACACTGTAACCTATCTCATAACCAATAGGGGCGGCGTCAGCCGCCCTTACTTTTT
GATTGAATGGCAAATATATT

Downstream 100 bases:

>100_bases
TGGCACGTCGTTCTCGCGGTCGTTTTATCGACGGTATTGTACTGTTAGATAAAGACACTGGCATGAGCTCTAACTTTGCA
TTACAAAGAGTGAAACGCTT

Product: ribosome-binding factor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MAKEFSRTRRIAQQLQQELAQVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVYVTFFEEDEKLVEQKVAALDAAAGYIRSL
VAGRMKLRVMPELRFIYDSSLVEGMRMSNLVSRVISNDEAKQKQHGVETDAEQGDTKEEGDK

Sequences:

>Translated_142_residues
MAKEFSRTRRIAQQLQQELAQVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVYVTFFEEDEKLVEQKVAALDAAAGYIRSL
VAGRMKLRVMPELRFIYDSSLVEGMRMSNLVSRVISNDEAKQKQHGVETDAEQGDTKEEGDK
>Mature_141_residues
AKEFSRTRRIAQQLQQELAQVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVYVTFFEEDEKLVEQKVAALDAAAGYIRSLV
AGRMKLRVMPELRFIYDSSLVEGMRMSNLVSRVISNDEAKQKQHGVETDAEQGDTKEEGDK

Specific function: Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA [H]

COG id: COG0858

COG function: function code J; Ribosome-binding factor A

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rbfA family [H]

Homologues:

Organism=Escherichia coli, GI1789558, Length=124, Percent_Identity=56.4516129032258, Blast_Score=145, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015946
- InterPro:   IPR000238
- InterPro:   IPR020053
- ProDom:   PD007327 [H]

Pfam domain/function: PF02033 RBFA [H]

EC number: NA

Molecular weight: Translated: 16204; Mature: 16072

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEFSRTRRIAQQLQQELAQVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVYVTFFEEDE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
KLVEQKVAALDAAAGYIRSLVAGRMKLRVMPELRFIYDSSLVEGMRMSNLVSRVISNDEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
KQKQHGVETDAEQGDTKEEGDK
HHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKEFSRTRRIAQQLQQELAQVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVYVTFFEEDE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
KLVEQKVAALDAAAGYIRSLVAGRMKLRVMPELRFIYDSSLVEGMRMSNLVSRVISNDEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
KQKQHGVETDAEQGDTKEEGDK
HHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA