Click here to switch to the map view.

The map label for this gene is truB [H]

Identifier: 157962876

GI number: 157962876

Start: 3751683

End: 3752630

Strand: Reverse

Name: truB [H]

Synonym: Spea_3058

Alternate gene names: 157962876

Gene position: 3752630-3751683 (Counterclockwise)

Preceding gene: 157962877

Following gene: 157962875

Centisome position: 72.52

GC content: 44.73

Gene sequence:

>948_bases
ATGGCACGTCGTTCTCGCGGTCGTTTTATCGACGGTATTGTACTGTTAGATAAAGACACTGGCATGAGCTCTAACTTTGC
ATTACAAAGAGTGAAACGCTTCTTTAATGCAAATAAAGCGGGTCATACCGGTGCACTGGATCCGCTAGCAACGGGCATGT
TACCTATTTGCTTAGGTGAGGCGACTAAGTTTTCGCAGCACTTACTCGACGCGGACAAACGCTACTTAGTAACGGCAAAG
CTTGGACAACGTACTGATACAAGTGACTCTGATGGTGAAATTGTTCAAACGAGAGAAGTTAACTTCACTCAAGAGCAGTT
AGATACTGCGCTGGAATATTTTCGCGGTGAGACGATGCAAGTCCCGTCAATGTATTCAGCACTAAAGTACCAAGGTCAGC
CTTTGTATAAGTACGCTCGTGAGGGAATTGAAGTTCCTCGTGAGGCACGCCCTATCAATGTGTTTGAGTTGAACTTTATT
AGCCTTGAAGGTGATGAGTTAACCTTAGACATTCATTGTTCTAAAGGTACCTATATCCGTACTATCACAGATGATTTAGG
CGAGATGTTAGGCTGTGGCGCACACGTTATCATGCTACGTCGCACACAAGTTGCTGGCTACCCCTATGATAAAATGGTGT
CATTGAAACAACTTGAAGAGTTAGTGGCTAAGGCTGATGCCGAGTCCTTGACTCTATCTGAGGTATTGGACCCATTACTG
TTACCTATGGATACTGCGGTAAGTGGTTTCAAAGAGATAAATGTCTCTGATGAACTTGCTACTTACCTGATGAATGGTAA
CCCTGTGCAGGTGGCTAATTTGCCTGTCGATGAGTTAGTGCGTATCACGATTGGCGATGCTCGTCAGTTTGTTGGTATCG
GACAAATGAACGATGATGGTCTGCTTGCTCCCAAGCGACTCATCGTATTAAGAGAGCCACAAGACTAA

Upstream 100 bases:

>100_bases
TAGTTAGCCGCGTGATCAGTAACGACGAAGCTAAGCAGAAGCAACACGGCGTAGAAACCGACGCTGAACAAGGCGACACT
AAGGAAGAGGGCGATAAGTA

Downstream 100 bases:

>100_bases
TTGTGGACCTCTTCCTAGATTAGTCTTGCTACCCAAAGTGTAAATGGGTAGAATGGCGCGCCCCTTTGGCTGAGTTAGTG
ATCGGCTAAAGATTTTTTAA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MARRSRGRFIDGIVLLDKDTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPICLGEATKFSQHLLDADKRYLVTAK
LGQRTDTSDSDGEIVQTREVNFTQEQLDTALEYFRGETMQVPSMYSALKYQGQPLYKYAREGIEVPREARPINVFELNFI
SLEGDELTLDIHCSKGTYIRTITDDLGEMLGCGAHVIMLRRTQVAGYPYDKMVSLKQLEELVAKADAESLTLSEVLDPLL
LPMDTAVSGFKEINVSDELATYLMNGNPVQVANLPVDELVRITIGDARQFVGIGQMNDDGLLAPKRLIVLREPQD

Sequences:

>Translated_315_residues
MARRSRGRFIDGIVLLDKDTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPICLGEATKFSQHLLDADKRYLVTAK
LGQRTDTSDSDGEIVQTREVNFTQEQLDTALEYFRGETMQVPSMYSALKYQGQPLYKYAREGIEVPREARPINVFELNFI
SLEGDELTLDIHCSKGTYIRTITDDLGEMLGCGAHVIMLRRTQVAGYPYDKMVSLKQLEELVAKADAESLTLSEVLDPLL
LPMDTAVSGFKEINVSDELATYLMNGNPVQVANLPVDELVRITIGDARQFVGIGQMNDDGLLAPKRLIVLREPQD
>Mature_314_residues
ARRSRGRFIDGIVLLDKDTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPICLGEATKFSQHLLDADKRYLVTAKL
GQRTDTSDSDGEIVQTREVNFTQEQLDTALEYFRGETMQVPSMYSALKYQGQPLYKYAREGIEVPREARPINVFELNFIS
LEGDELTLDIHCSKGTYIRTITDDLGEMLGCGAHVIMLRRTQVAGYPYDKMVSLKQLEELVAKADAESLTLSEVLDPLLL
PMDTAVSGFKEINVSDELATYLMNGNPVQVANLPVDELVRITIGDARQFVGIGQMNDDGLLAPKRLIVLREPQD

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=187, Percent_Identity=36.8983957219251, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI215599015, Length=181, Percent_Identity=28.1767955801105, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4503337, Length=181, Percent_Identity=28.1767955801105, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI2367200, Length=310, Percent_Identity=63.2258064516129, Blast_Score=413, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17553978, Length=320, Percent_Identity=25.625, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=321, Percent_Identity=27.1028037383178, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324037, Length=151, Percent_Identity=38.4105960264901, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281364189, Length=192, Percent_Identity=28.6458333333333, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364187, Length=192, Percent_Identity=28.6458333333333, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364185, Length=192, Percent_Identity=28.6458333333333, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364183, Length=192, Percent_Identity=28.6458333333333, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62471759, Length=192, Percent_Identity=28.6458333333333, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17975520, Length=192, Percent_Identity=28.6458333333333, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35040; Mature: 34908

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRSRGRFIDGIVLLDKDTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPICLGE
CCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCC
ATKFSQHLLDADKRYLVTAKLGQRTDTSDSDGEIVQTREVNFTQEQLDTALEYFRGETMQ
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEE
VPSMYSALKYQGQPLYKYAREGIEVPREARPINVFELNFISLEGDELTLDIHCSKGTYIR
CCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCEEEEEEEECCCCEEE
TITDDLGEMLGCGAHVIMLRRTQVAGYPYDKMVSLKQLEELVAKADAESLTLSEVLDPLL
EHHHHHHHHHCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
LPMDTAVSGFKEINVSDELATYLMNGNPVQVANLPVDELVRITIGDARQFVGIGQMNDDG
CCHHHHHCCHHHCCCHHHHHHHHCCCCEEEEECCCHHHEEEEEECCCHHEEECCCCCCCC
LLAPKRLIVLREPQD
CCCCCEEEEEECCCC
>Mature Secondary Structure 
ARRSRGRFIDGIVLLDKDTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPICLGE
CCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCC
ATKFSQHLLDADKRYLVTAKLGQRTDTSDSDGEIVQTREVNFTQEQLDTALEYFRGETMQ
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEE
VPSMYSALKYQGQPLYKYAREGIEVPREARPINVFELNFISLEGDELTLDIHCSKGTYIR
CCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCEEEEEEEECCCCEEE
TITDDLGEMLGCGAHVIMLRRTQVAGYPYDKMVSLKQLEELVAKADAESLTLSEVLDPLL
EHHHHHHHHHCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
LPMDTAVSGFKEINVSDELATYLMNGNPVQVANLPVDELVRITIGDARQFVGIGQMNDDG
CCHHHHHCCHHHCCCHHHHHHHHCCCCEEEEECCCHHHEEEEEECCCHHEEECCCCCCCC
LLAPKRLIVLREPQD
CCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA