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The map label for this gene is radA [H]

Identifier: 157962850

GI number: 157962850

Start: 3716761

End: 3718134

Strand: Reverse

Name: radA [H]

Synonym: Spea_3032

Alternate gene names: 157962850

Gene position: 3718134-3716761 (Counterclockwise)

Preceding gene: 157962852

Following gene: 157962846

Centisome position: 71.85

GC content: 45.92

Gene sequence:

>1374_bases
ATGGCAAAGAATAAAACAGCTTATGTATGTAATGAGTGTGGTCAAGATTTTCCTCGTTGGCAGGGGCAATGTAGTGCCTG
TCAGGAGTGGAATACCATCAGTGAACTGAAAATTTCGGCGCTAAAACCTGCTCGTGCTTCGGTCTCAGGCTATGCTGGCT
CTGTGGGCGGTGGTTCGAAAAAGTTAACCGAAATCGAAGAGTTTGAAGCGCAAAAGATGCTGACAGGCATTGGCGAGCTT
GACCGAGTGCTTTGTGGCGGATTGACCACAGGTTCGGTAAACATCATTTCTGGTGACCCTGGTGCAGGTAAGACCACGAT
TTTATCAGATCTGGTGTCGCGTATGTCGCAGTTAATGCCATCGCTTTATTGTACCGCAGAGGAGTCGTTATCTCAGTTCA
AAAACCGAGTTAACCGTCTAAAGCTAGATTGCAATGAAGACAACTTGTACCTGATGGCCGAAACCAGTGTCGAAGCCATT
ATTGCCGAACTTGAAGAGAAGAAGATTAAGTTTGCGGTTATTGACTCTATTCAGGCTGTTGTGACCGATTTAGCTAACGG
CAGTCCGGGCTCGCCTTCACAGGTTAAGAGTAGTGCCCAGGCTCTCACTCAGTATTGTAAGCAAAATAACGTCACCATGT
TTTTAGTGGCTCACGTTAACAAGAATAATGAAACTGCCGGGCCACAGACCCTAATCCATATCGTCGATTGTTTAACTCAT
ATCGAGTGTAACGACGGCCAAATTCGCACACTTCGAGCCAACAAAAACCGCTTCGGCGATGTCGATACCGTGGGCATTTT
TAAGATGACCGAACGCGGCATGATAAGTGTTGATAATCCGAGTGAGATATTTCTTTCCGGCTCCACAACAGAGTCACCCG
GCGCGGCAATTACCTGTATCCGTAAGGGCAATCGCAATTTATTACTCGAGATCCAGTGCCTAACCACAGAGACCGAAGCT
GAGTTTCCGCAGCGAGTTTGCGTAGGTTTGAATATGAACCGTATCAAGATGCTAACTGGGATATTGCGTAAGCACACTAA
GACCAAAATTTTCCACGATACCTATTTTAACTTGGTCGGTGGTCTTAAAATTGATGAGTCTGAAACTTGCATCGACTTAG
CCCTAGTGACCGCACTGCTCAGTAGTTTGAATGATTTTGTGGTGCCAAGAACCACATGCATCATGGGGGAGCTGAGTTTA
AATGGGGATGTTCGCCCAATTGACAGTGGTGTTCCGCGAGTTAAAGAGGCGATTCAACATGGTTTTACCGAGATTTATAT
TCCATTTCGTAATTACCATAAGTCGATGGAAGGCTCGGGAGCCAAGATTATTCCGGTGAAGACGATCCATGAACTGCTGG
AGTTAATAAACTAG

Upstream 100 bases:

>100_bases
GGCATTTTTCAAGGGAGTAGTCGACTACTCTGCTTATCTTTAATTAGTATCGGCCAATATTGGCATTACTTTACCGATTT
AATACGATTTAGAAAGAGCT

Downstream 100 bases:

>100_bases
CGAGTGATAACGTTAAGCGTGAAGGCCGCCTAAAACTTTGATTGTAAAAACGCCGCAATACTGCGGCGTTTTTACTTAAG
GCTTGGGAGTGTCGCAGCCA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MAKNKTAYVCNECGQDFPRWQGQCSACQEWNTISELKISALKPARASVSGYAGSVGGGSKKLTEIEEFEAQKMLTGIGEL
DRVLCGGLTTGSVNIISGDPGAGKTTILSDLVSRMSQLMPSLYCTAEESLSQFKNRVNRLKLDCNEDNLYLMAETSVEAI
IAELEEKKIKFAVIDSIQAVVTDLANGSPGSPSQVKSSAQALTQYCKQNNVTMFLVAHVNKNNETAGPQTLIHIVDCLTH
IECNDGQIRTLRANKNRFGDVDTVGIFKMTERGMISVDNPSEIFLSGSTTESPGAAITCIRKGNRNLLLEIQCLTTETEA
EFPQRVCVGLNMNRIKMLTGILRKHTKTKIFHDTYFNLVGGLKIDESETCIDLALVTALLSSLNDFVVPRTTCIMGELSL
NGDVRPIDSGVPRVKEAIQHGFTEIYIPFRNYHKSMEGSGAKIIPVKTIHELLELIN

Sequences:

>Translated_457_residues
MAKNKTAYVCNECGQDFPRWQGQCSACQEWNTISELKISALKPARASVSGYAGSVGGGSKKLTEIEEFEAQKMLTGIGEL
DRVLCGGLTTGSVNIISGDPGAGKTTILSDLVSRMSQLMPSLYCTAEESLSQFKNRVNRLKLDCNEDNLYLMAETSVEAI
IAELEEKKIKFAVIDSIQAVVTDLANGSPGSPSQVKSSAQALTQYCKQNNVTMFLVAHVNKNNETAGPQTLIHIVDCLTH
IECNDGQIRTLRANKNRFGDVDTVGIFKMTERGMISVDNPSEIFLSGSTTESPGAAITCIRKGNRNLLLEIQCLTTETEA
EFPQRVCVGLNMNRIKMLTGILRKHTKTKIFHDTYFNLVGGLKIDESETCIDLALVTALLSSLNDFVVPRTTCIMGELSL
NGDVRPIDSGVPRVKEAIQHGFTEIYIPFRNYHKSMEGSGAKIIPVKTIHELLELIN
>Mature_456_residues
AKNKTAYVCNECGQDFPRWQGQCSACQEWNTISELKISALKPARASVSGYAGSVGGGSKKLTEIEEFEAQKMLTGIGELD
RVLCGGLTTGSVNIISGDPGAGKTTILSDLVSRMSQLMPSLYCTAEESLSQFKNRVNRLKLDCNEDNLYLMAETSVEAII
AELEEKKIKFAVIDSIQAVVTDLANGSPGSPSQVKSSAQALTQYCKQNNVTMFLVAHVNKNNETAGPQTLIHIVDCLTHI
ECNDGQIRTLRANKNRFGDVDTVGIFKMTERGMISVDNPSEIFLSGSTTESPGAAITCIRKGNRNLLLEIQCLTTETEAE
FPQRVCVGLNMNRIKMLTGILRKHTKTKIFHDTYFNLVGGLKIDESETCIDLALVTALLSSLNDFVVPRTTCIMGELSLN
GDVRPIDSGVPRVKEAIQHGFTEIYIPFRNYHKSMEGSGAKIIPVKTIHELLELIN

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=464, Percent_Identity=42.8879310344828, Blast_Score=370, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49996; Mature: 49865

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKTAYVCNECGQDFPRWQGQCSACQEWNTISELKISALKPARASVSGYAGSVGGGSK
CCCCCCCHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCCH
KLTEIEEFEAQKMLTGIGELDRVLCGGLTTGSVNIISGDPGAGKTTILSDLVSRMSQLMP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
SLYCTAEESLSQFKNRVNRLKLDCNEDNLYLMAETSVEAIIAELEEKKIKFAVIDSIQAV
HHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCHHHHHHHHHHHHHEEHHHHHHHHHH
VTDLANGSPGSPSQVKSSAQALTQYCKQNNVTMFLVAHVNKNNETAGPQTLIHIVDCLTH
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHH
IECNDGQIRTLRANKNRFGDVDTVGIFKMTERGMISVDNPSEIFLSGSTTESPGAAITCI
CCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEECCCCEEEEECCCCCCCCCEEEEE
RKGNRNLLLEIQCLTTETEAEFPQRVCVGLNMNRIKMLTGILRKHTKTKIFHDTYFNLVG
EECCCCEEEEEEEECCCHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLKIDESETCIDLALVTALLSSLNDFVVPRTTCIMGELSLNGDVRPIDSGVPRVKEAIQH
CCEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHC
GFTEIYIPFRNYHKSMEGSGAKIIPVKTIHELLELIN
CCEEEEEEHHHHHHHCCCCCCEEEEHHHHHHHHHHHC
>Mature Secondary Structure 
AKNKTAYVCNECGQDFPRWQGQCSACQEWNTISELKISALKPARASVSGYAGSVGGGSK
CCCCCCHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCCH
KLTEIEEFEAQKMLTGIGELDRVLCGGLTTGSVNIISGDPGAGKTTILSDLVSRMSQLMP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
SLYCTAEESLSQFKNRVNRLKLDCNEDNLYLMAETSVEAIIAELEEKKIKFAVIDSIQAV
HHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCHHHHHHHHHHHHHEEHHHHHHHHHH
VTDLANGSPGSPSQVKSSAQALTQYCKQNNVTMFLVAHVNKNNETAGPQTLIHIVDCLTH
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHH
IECNDGQIRTLRANKNRFGDVDTVGIFKMTERGMISVDNPSEIFLSGSTTESPGAAITCI
CCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEECCCCEEEEECCCCCCCCCEEEEE
RKGNRNLLLEIQCLTTETEAEFPQRVCVGLNMNRIKMLTGILRKHTKTKIFHDTYFNLVG
EECCCCEEEEEEEECCCHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLKIDESETCIDLALVTALLSSLNDFVVPRTTCIMGELSLNGDVRPIDSGVPRVKEAIQH
CCEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHC
GFTEIYIPFRNYHKSMEGSGAKIIPVKTIHELLELIN
CCEEEEEEHHHHHHHCCCCCCEEEEHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]