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The map label for this gene is cpsA1 [H]

Identifier: 157962746

GI number: 157962746

Start: 3582553

End: 3583878

Strand: Reverse

Name: cpsA1 [H]

Synonym: Spea_2928

Alternate gene names: 157962746

Gene position: 3583878-3582553 (Counterclockwise)

Preceding gene: 157962748

Following gene: 157962740

Centisome position: 69.26

GC content: 47.59

Gene sequence:

>1326_bases
ATGAGCTTACTAAAAGTATCTTCGCTTGCACTACTCATTGGGTGTAGTTTGGCAACCCCGTGTCATGCAACTAATTTAGC
CGACTCTACGGCCAAGGCCATGCCTAACCTAGAAGCCTTATACCTGCATCTACACCAAAACCCGGAGCTGTCTTATCAAG
AAAAAGCCAGTAGCGCTCGTATAGCCAAAGAGTTAGCAAAATTGGGTTTCGAGGTTACCGAAAATTTTGGTGGTTATGGC
GTAGTAGGTGTCCTTAAAAATGGCGAGGGGCCAACGGTGATGATCCGCGCCGATACCGATGCTTTGCCGATAACGGAAGA
AACGGGCAAGTCTTACGCCTCTAAAGTCATCACAACAAATGCCAATAACCAAGAGGTGGGAGTCATGCACGGCTGTGGTC
ATGATATCCATATGACGAGTTTGGTGGGTACGGCTCAGCAGCTGGTGGCCAATAAAGCCAATTGGCAAGGTACCTTGATG
ATGGTCGCACAGCCTGCTGAAGAGGTTGGCGGCGGTGCAAAAGCCATGTTGAAGCAGGGGCTATTTACTGATTTTGATAC
GCCTGATTATATTCTCGGCTTGCACGTTAGCGCATCAATACCTGCTGGTAAGGTGGGGATAGTCTCTGGCTATGCCTTAG
CGAATGTAGACTCAGTCGATATCACAGTTAAAGGCGAGGGGGGTCACGGCGCATATCCTCATCTAACCAAAGATCCTGTG
GTATTAGCCTCGAGAATTGTATTAGCGTTACAAACGATTGCCAGCCGCGAGTTATCGCCATTAGAGCCTAGCGTGATAAC
TGTCGGCTCGATTCATGGCGGTTCTAAGCACAATATTATCTCTAATGAAGTTAAATTACAGCTTACTCTGCGCTCCTATA
ATCCAGAGGTTAGGCTGCAACAAATAGCCGCACTTAAGCGATTAACCAAAGGAATTGCCATGAGCGCAGGCCTTGATGAA
TCTTTGTATCCTGAGGTGTATGTCCATGAAGAGGAGCGGATCCCATCAACCTATAATGATCCTGTGTTAGTGAGCCGAGT
GCGAGCGAGTATTGAGTCTGAGATGGGTGATGATAATGTGATAGATGCCTTGCCTGTTATGGCGGGCGAAGATTTTGGGC
TTTACGGGTTAACCGAGGATAAACGGCCAATCACGATTTTTTGGTTAGGTGCTGTAGAGCCGAGTGAATATCAATCTAGC
CTAGACACGGGCCATGCTTTACCGTCACTGCACTCCAGTAAATTTGCACCGGATTACCCTGTAACGATTAGTACCGGGGT
TAGATCCATGAGCAAAGCCGCGATTGACTTATTCAATCAGCCATAA

Upstream 100 bases:

>100_bases
TCTCACTTGGCATATAAGTGTAGAGCTGTTTTAGTTTCAGTAAATAGGTGTCGCGCGCATGAAATTAATGGCCTATAAAA
AATAATAGTAGAGGAATATC

Downstream 100 bases:

>100_bases
GCAGAAATGTAATCTTCATCTGTGTCTTAGCTTATTTGCTTAAAGCTTGCTTTGAGGCTAAAGGGGAGGACATGAATTAA
CGTATACGCTGCAGCTTATT

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSLLKVSSLALLIGCSLATPCHATNLADSTAKAMPNLEALYLHLHQNPELSYQEKASSARIAKELAKLGFEVTENFGGYG
VVGVLKNGEGPTVMIRADTDALPITEETGKSYASKVITTNANNQEVGVMHGCGHDIHMTSLVGTAQQLVANKANWQGTLM
MVAQPAEEVGGGAKAMLKQGLFTDFDTPDYILGLHVSASIPAGKVGIVSGYALANVDSVDITVKGEGGHGAYPHLTKDPV
VLASRIVLALQTIASRELSPLEPSVITVGSIHGGSKHNIISNEVKLQLTLRSYNPEVRLQQIAALKRLTKGIAMSAGLDE
SLYPEVYVHEEERIPSTYNDPVLVSRVRASIESEMGDDNVIDALPVMAGEDFGLYGLTEDKRPITIFWLGAVEPSEYQSS
LDTGHALPSLHSSKFAPDYPVTISTGVRSMSKAAIDLFNQP

Sequences:

>Translated_441_residues
MSLLKVSSLALLIGCSLATPCHATNLADSTAKAMPNLEALYLHLHQNPELSYQEKASSARIAKELAKLGFEVTENFGGYG
VVGVLKNGEGPTVMIRADTDALPITEETGKSYASKVITTNANNQEVGVMHGCGHDIHMTSLVGTAQQLVANKANWQGTLM
MVAQPAEEVGGGAKAMLKQGLFTDFDTPDYILGLHVSASIPAGKVGIVSGYALANVDSVDITVKGEGGHGAYPHLTKDPV
VLASRIVLALQTIASRELSPLEPSVITVGSIHGGSKHNIISNEVKLQLTLRSYNPEVRLQQIAALKRLTKGIAMSAGLDE
SLYPEVYVHEEERIPSTYNDPVLVSRVRASIESEMGDDNVIDALPVMAGEDFGLYGLTEDKRPITIFWLGAVEPSEYQSS
LDTGHALPSLHSSKFAPDYPVTISTGVRSMSKAAIDLFNQP
>Mature_440_residues
SLLKVSSLALLIGCSLATPCHATNLADSTAKAMPNLEALYLHLHQNPELSYQEKASSARIAKELAKLGFEVTENFGGYGV
VGVLKNGEGPTVMIRADTDALPITEETGKSYASKVITTNANNQEVGVMHGCGHDIHMTSLVGTAQQLVANKANWQGTLMM
VAQPAEEVGGGAKAMLKQGLFTDFDTPDYILGLHVSASIPAGKVGIVSGYALANVDSVDITVKGEGGHGAYPHLTKDPVV
LASRIVLALQTIASRELSPLEPSVITVGSIHGGSKHNIISNEVKLQLTLRSYNPEVRLQQIAALKRLTKGIAMSAGLDES
LYPEVYVHEEERIPSTYNDPVLVSRVRASIESEMGDDNVIDALPVMAGEDFGLYGLTEDKRPITIFWLGAVEPSEYQSSL
DTGHALPSLHSSKFAPDYPVTISTGVRSMSKAAIDLFNQP

Specific function: Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=251, Percent_Identity=30.6772908366534, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 47015; Mature: 46884

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLKVSSLALLIGCSLATPCHATNLADSTAKAMPNLEALYLHLHQNPELSYQEKASSAR
CCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHH
IAKELAKLGFEVTENFGGYGVVGVLKNGEGPTVMIRADTDALPITEETGKSYASKVITTN
HHHHHHHCCHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHEEEEEC
ANNQEVGVMHGCGHDIHMTSLVGTAQQLVANKANWQGTLMMVAQPAEEVGGGAKAMLKQG
CCCCEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHCCCHHHHHHHC
LFTDFDTPDYILGLHVSASIPAGKVGIVSGYALANVDSVDITVKGEGGHGAYPHLTKDPV
CCCCCCCCCEEEEEEEECCCCCCCEEEEECEEEECCCEEEEEEEECCCCCCCCCCCCCHH
VLASRIVLALQTIASRELSPLEPSVITVGSIHGGSKHNIISNEVKLQLTLRSYNPEVRLQ
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEECCCEEEEEEEECCCCCHHHH
QIAALKRLTKGIAMSAGLDESLYPEVYVHEEERIPSTYNDPVLVSRVRASIESEMGDDNV
HHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
IDALPVMAGEDFGLYGLTEDKRPITIFWLGAVEPSEYQSSLDTGHALPSLHSSKFAPDYP
HHHHHHCCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
VTISTGVRSMSKAAIDLFNQP
EEEHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLLKVSSLALLIGCSLATPCHATNLADSTAKAMPNLEALYLHLHQNPELSYQEKASSAR
CCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHH
IAKELAKLGFEVTENFGGYGVVGVLKNGEGPTVMIRADTDALPITEETGKSYASKVITTN
HHHHHHHCCHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHEEEEEC
ANNQEVGVMHGCGHDIHMTSLVGTAQQLVANKANWQGTLMMVAQPAEEVGGGAKAMLKQG
CCCCEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHCCCHHHHHHHC
LFTDFDTPDYILGLHVSASIPAGKVGIVSGYALANVDSVDITVKGEGGHGAYPHLTKDPV
CCCCCCCCCEEEEEEEECCCCCCCEEEEECEEEECCCEEEEEEEECCCCCCCCCCCCCHH
VLASRIVLALQTIASRELSPLEPSVITVGSIHGGSKHNIISNEVKLQLTLRSYNPEVRLQ
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEECCCEEEEEEEECCCCCHHHH
QIAALKRLTKGIAMSAGLDESLYPEVYVHEEERIPSTYNDPVLVSRVRASIESEMGDDNV
HHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
IDALPVMAGEDFGLYGLTEDKRPITIFWLGAVEPSEYQSSLDTGHALPSLHSSKFAPDYP
HHHHHHCCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
VTISTGVRSMSKAAIDLFNQP
EEEHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559343; 11427726; 1597179 [H]