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The map label for this gene is truC [H]

Identifier: 157962710

GI number: 157962710

Start: 3530330

End: 3531184

Strand: Reverse

Name: truC [H]

Synonym: Spea_2891

Alternate gene names: 157962710

Gene position: 3531184-3530330 (Counterclockwise)

Preceding gene: 157962711

Following gene: 157962709

Centisome position: 68.24

GC content: 46.08

Gene sequence:

>855_bases
ATGAGTGATAACACTAAAAATCCCTCTCAAAGTGAACAACTTGATGAGAGCCTACTCGAACAAGACTTATGTCAAGATGA
AGAACAAGAGATAGCGCCGCATATCGAAGTGCTATTTGAAGATGATGATATTGTGGTGATCCACAAGCCTGCAGGCTTGT
TGGTGCACCGCAGTTATCTTGCCAGACGTGAGCGATTCTTTGCTATGCAACTGACTCGAGATCTAGTTGGTTGTCACGTA
TTCCCGGTGCATAGGCTAGATAGACCGACGTCAGGTATTTTGTTGTTTGCCAAAAGCAGTGAAATGGCTAATGTCCTGTG
TGAGCAGTTTGCTGCTCATACGGTCGATAAGGAGTATCTAGCGATTGTTCGTGGCAACATGCATGAGGCGGGTACGCTAG
ACTATGCCCTCAAAGAGGAGCTTGATGAGCTAGGCGATAAAGATGTGGATCCTAACAAGGCTGCGCAAGATGCAGTAACT
AGCTATAAGCCATTATTGAACAGTGAGATCCCTTTCTCTTCGGGTCGTTATGCAACCAGTCGTTACGCTTTAGTTCAACT
TTCTCCTCACACTGGTCGTAAGCATCAATTAAGACGTCATATGGCACATTTACGCCACCCTATTCTGGGTGATACTACTC
ATGGCGACGGTAAACAGAATAAGTTCTTCCGTTCTCATTTTGGCATCAATAGATTGTGGCTAATCGCCAAGAAACTGAGC
TTTAATCACCCTAGGAGCGGTGAGCGCATGAGCGTAGAGACCGAGCTTGAGGCGGAGTGGTTAAGTTTGTTTGAAGGTCT
AGGTTGGCATGAGGAGACACTATCCCTGGACGCTCAGCCGATTATTATAGCCTGA

Upstream 100 bases:

>100_bases
GGGCCGCTAACATTGAGTATAAGCGAGTCATTCAGCTGCTCACTGAGCTAGATGCATTGTTGAGTCATGCACCATAACGG
TATTACAAGAGATATATACA

Downstream 100 bases:

>100_bases
TAGTCGCAATCAGTAATCGGCTTAGCAGTTATAGCTAAAAACCTAGTGAAATAGCACTATATTTTCTATTGGAACTATGA
TTTCTGTTGAGTGTTATCTT

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSDNTKNPSQSEQLDESLLEQDLCQDEEQEIAPHIEVLFEDDDIVVIHKPAGLLVHRSYLARRERFFAMQLTRDLVGCHV
FPVHRLDRPTSGILLFAKSSEMANVLCEQFAAHTVDKEYLAIVRGNMHEAGTLDYALKEELDELGDKDVDPNKAAQDAVT
SYKPLLNSEIPFSSGRYATSRYALVQLSPHTGRKHQLRRHMAHLRHPILGDTTHGDGKQNKFFRSHFGINRLWLIAKKLS
FNHPRSGERMSVETELEAEWLSLFEGLGWHEETLSLDAQPIIIA

Sequences:

>Translated_284_residues
MSDNTKNPSQSEQLDESLLEQDLCQDEEQEIAPHIEVLFEDDDIVVIHKPAGLLVHRSYLARRERFFAMQLTRDLVGCHV
FPVHRLDRPTSGILLFAKSSEMANVLCEQFAAHTVDKEYLAIVRGNMHEAGTLDYALKEELDELGDKDVDPNKAAQDAVT
SYKPLLNSEIPFSSGRYATSRYALVQLSPHTGRKHQLRRHMAHLRHPILGDTTHGDGKQNKFFRSHFGINRLWLIAKKLS
FNHPRSGERMSVETELEAEWLSLFEGLGWHEETLSLDAQPIIIA
>Mature_283_residues
SDNTKNPSQSEQLDESLLEQDLCQDEEQEIAPHIEVLFEDDDIVVIHKPAGLLVHRSYLARRERFFAMQLTRDLVGCHVF
PVHRLDRPTSGILLFAKSSEMANVLCEQFAAHTVDKEYLAIVRGNMHEAGTLDYALKEELDELGDKDVDPNKAAQDAVTS
YKPLLNSEIPFSSGRYATSRYALVQLSPHTGRKHQLRRHMAHLRHPILGDTTHGDGKQNKFFRSHFGINRLWLIAKKLSF
NHPRSGERMSVETELEAEWLSLFEGLGWHEETLSLDAQPIIIA

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI23308689, Length=205, Percent_Identity=31.219512195122, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI221316667, Length=227, Percent_Identity=30.3964757709251, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221316663, Length=245, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI17158025, Length=145, Percent_Identity=34.4827586206897, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1789155, Length=235, Percent_Identity=50.6382978723404, Blast_Score=254, Evalue=3e-69,
Organism=Escherichia coli, GI1787327, Length=248, Percent_Identity=33.4677419354839, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1788946, Length=255, Percent_Identity=27.4509803921569, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786244, Length=220, Percent_Identity=31.8181818181818, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17534965, Length=183, Percent_Identity=30.0546448087432, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320168, Length=185, Percent_Identity=34.5945945945946, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324506, Length=204, Percent_Identity=32.843137254902, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321608, Length=217, Percent_Identity=29.4930875576037, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24583449, Length=177, Percent_Identity=31.0734463276836, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583447, Length=177, Percent_Identity=31.0734463276836, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583445, Length=177, Percent_Identity=31.0734463276836, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19921090, Length=182, Percent_Identity=31.8681318681319, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32358; Mature: 32227

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNTKNPSQSEQLDESLLEQDLCQDEEQEIAPHIEVLFEDDDIVVIHKPAGLLVHRSYL
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEECCCEEEEECCCCHHHHHHHH
ARRERFFAMQLTRDLVGCHVFPVHRLDRPTSGILLFAKSSEMANVLCEQFAAHTVDKEYL
HHHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHH
AIVRGNMHEAGTLDYALKEELDELGDKDVDPNKAAQDAVTSYKPLLNSEIPFSSGRYATS
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RYALVQLSPHTGRKHQLRRHMAHLRHPILGDTTHGDGKQNKFFRSHFGINRLWLIAKKLS
EEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHC
FNHPRSGERMSVETELEAEWLSLFEGLGWHEETLSLDAQPIIIA
CCCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEC
>Mature Secondary Structure 
SDNTKNPSQSEQLDESLLEQDLCQDEEQEIAPHIEVLFEDDDIVVIHKPAGLLVHRSYL
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEECCCEEEEECCCCHHHHHHHH
ARRERFFAMQLTRDLVGCHVFPVHRLDRPTSGILLFAKSSEMANVLCEQFAAHTVDKEYL
HHHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHH
AIVRGNMHEAGTLDYALKEELDELGDKDVDPNKAAQDAVTSYKPLLNSEIPFSSGRYATS
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RYALVQLSPHTGRKHQLRRHMAHLRHPILGDTTHGDGKQNKFFRSHFGINRLWLIAKKLS
EEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHC
FNHPRSGERMSVETELEAEWLSLFEGLGWHEETLSLDAQPIIIA
CCCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA