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The map label for this gene is tsf [H]

Identifier: 157962703

GI number: 157962703

Start: 3522367

End: 3523278

Strand: Reverse

Name: tsf [H]

Synonym: Spea_2884

Alternate gene names: 157962703

Gene position: 3523278-3522367 (Counterclockwise)

Preceding gene: 157962704

Following gene: 157962702

Centisome position: 68.09

GC content: 42.87

Gene sequence:

>912_bases
ATGATTGGCCCCTGTTTCTTATCTATTGAATTTGAAAAATTATCTGTAGAGGATTTAGCAATGGCAATTACTGCTGCCCA
AGTTAAAGAACTTCGTGACCGCACTGGCGCTGGCATGATGGACTGTAAAAAAGCACTGACTGAAACTAACGGTGACATCG
AGTTAGCAATTGATAACATGCGTAAGAGCGGTGCTGCTAAGGCTGCTAAAAAAGCGGGTAACATCGCTGCTGAAGGTACT
ATCCTTATCAAGCAAGGCGAAGGTTACGCTGCTCTACTAGAAGTTAACTGTCAAACTGACTTCGTTGCAAAAGATTCTAA
CTTCCTAGCATTTGCTAACGAAGTACTAGATGTTGCTGCAGCATCTAAAGTAACTATCGAAGCACTAAAAGAGCAGTTTG
AAGAAACTCGTGTTGCTCTAGTTGCTAAAATCGGTGAAAACATCAACGTACGTCGTGTTGAGTACATCGATGGTGCAAAC
CTAGCTGAATACCGTCACGGCGAGCGTATCGGTGTTGTTGTTGCCGGTGTTGCTGATGAAGAGACTCTAAAGCACGTTGC
TATGCACGTTGCTGCTTCTAAGCCTGAATACGTTAACCCTTCAGATGTTCCTGCGGAAGTTGTTGAGAAAGAAAAAGCTC
TTCAAATCGAAATCGCTATGAACGAAGGCAAGCCTGCTGAAATCGCTGAGAAGATGGTTATCGGTCGTATGAAGAAGTTC
ACTGGTGAGGTTTCTCTTACTGGTCAAGCTTTCATCATGGAACCAAAGAAAACTGTTGGCGAAATTCTTAAAGAGAAAGG
CGCTTCAGTAACTAACTTCATTCGTTTAGAAGTTGGTGAAGGCATCGAAAGAAAAGAAGAAGATTTTGCTGCTGAAGTAG
CTGCACAAATCGCTGCTACTACTAAGGCTTAA

Upstream 100 bases:

>100_bases
AAGACGGTTTCGTAGAAGCTGAATAATAACGGTCGATGAATTTTCATCACCCTTATTAACCAAGATACATGATTGGTGAA
CAGGGGCTCTTTCATTAAAT

Downstream 100 bases:

>100_bases
TCTCCTTATTTAGCAAACATAAGACCGTAGCAATTGCTGCGGTCTTATCGTATATACTCGAATAGTTTTTGTCTTGTTAA
GTAACATTCGGTAAAACTAT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIGPCFLSIEFEKLSVEDLAMAITAAQVKELRDRTGAGMMDCKKALTETNGDIELAIDNMRKSGAAKAAKKAGNIAAEGT
ILIKQGEGYAALLEVNCQTDFVAKDSNFLAFANEVLDVAAASKVTIEALKEQFEETRVALVAKIGENINVRRVEYIDGAN
LAEYRHGERIGVVVAGVADEETLKHVAMHVAASKPEYVNPSDVPAEVVEKEKALQIEIAMNEGKPAEIAEKMVIGRMKKF
TGEVSLTGQAFIMEPKKTVGEILKEKGASVTNFIRLEVGEGIERKEEDFAAEVAAQIAATTKA

Sequences:

>Translated_303_residues
MIGPCFLSIEFEKLSVEDLAMAITAAQVKELRDRTGAGMMDCKKALTETNGDIELAIDNMRKSGAAKAAKKAGNIAAEGT
ILIKQGEGYAALLEVNCQTDFVAKDSNFLAFANEVLDVAAASKVTIEALKEQFEETRVALVAKIGENINVRRVEYIDGAN
LAEYRHGERIGVVVAGVADEETLKHVAMHVAASKPEYVNPSDVPAEVVEKEKALQIEIAMNEGKPAEIAEKMVIGRMKKF
TGEVSLTGQAFIMEPKKTVGEILKEKGASVTNFIRLEVGEGIERKEEDFAAEVAAQIAATTKA
>Mature_303_residues
MIGPCFLSIEFEKLSVEDLAMAITAAQVKELRDRTGAGMMDCKKALTETNGDIELAIDNMRKSGAAKAAKKAGNIAAEGT
ILIKQGEGYAALLEVNCQTDFVAKDSNFLAFANEVLDVAAASKVTIEALKEQFEETRVALVAKIGENINVRRVEYIDGAN
LAEYRHGERIGVVVAGVADEETLKHVAMHVAASKPEYVNPSDVPAEVVEKEKALQIEIAMNEGKPAEIAEKMVIGRMKKF
TGEVSLTGQAFIMEPKKTVGEILKEKGASVTNFIRLEVGEGIERKEEDFAAEVAAQIAATTKA

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=282, Percent_Identity=24.822695035461, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1786366, Length=275, Percent_Identity=72, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17561440, Length=292, Percent_Identity=25, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 32632; Mature: 32632

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGPCFLSIEFEKLSVEDLAMAITAAQVKELRDRTGAGMMDCKKALTETNGDIELAIDNM
CCCEEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECH
RKSGAAKAAKKAGNIAAEGTILIKQGEGYAALLEVNCQTDFVAKDSNFLAFANEVLDVAA
HHCCHHHHHHHCCCEEECCEEEEEECCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHHH
ASKVTIEALKEQFEETRVALVAKIGENINVRRVEYIDGANLAEYRHGERIGVVVAGVADE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCEEEEEEEECCCH
ETLKHVAMHVAASKPEYVNPSDVPAEVVEKEKALQIEIAMNEGKPAEIAEKMVIGRMKKF
HHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
TGEVSLTGQAFIMEPKKTVGEILKEKGASVTNFIRLEVGEGIERKEEDFAAEVAAQIAAT
CCCEEECCCEEEECCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
TKA
CCC
>Mature Secondary Structure
MIGPCFLSIEFEKLSVEDLAMAITAAQVKELRDRTGAGMMDCKKALTETNGDIELAIDNM
CCCEEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECH
RKSGAAKAAKKAGNIAAEGTILIKQGEGYAALLEVNCQTDFVAKDSNFLAFANEVLDVAA
HHCCHHHHHHHCCCEEECCEEEEEECCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHHH
ASKVTIEALKEQFEETRVALVAKIGENINVRRVEYIDGANLAEYRHGERIGVVVAGVADE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCEEEEEEEECCCH
ETLKHVAMHVAASKPEYVNPSDVPAEVVEKEKALQIEIAMNEGKPAEIAEKMVIGRMKKF
HHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
TGEVSLTGQAFIMEPKKTVGEILKEKGASVTNFIRLEVGEGIERKEEDFAAEVAAQIAAT
CCCEEECCCEEEECCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
TKA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA