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The map label for this gene is tilS [H]

Identifier: 157962688

GI number: 157962688

Start: 3504115

End: 3505524

Strand: Reverse

Name: tilS [H]

Synonym: Spea_2869

Alternate gene names: 157962688

Gene position: 3505524-3504115 (Counterclockwise)

Preceding gene: 157962689

Following gene: 157962686

Centisome position: 67.75

GC content: 47.94

Gene sequence:

>1410_bases
ATGGATATCTCGGCTTCTATCTCAGAGTGCATGAAGTTGTTACCTCAGCGCTCAATAAACACCGAGGTAACAGGCGAACA
AGTGTGCCAGAAAAGGCGCAAGCTGGTGCTGGCGTACAGTGGTGGCGTCGACTCTGAAATATTGGCTCACGGCCTTTCAC
AGTTCGCTAAAGCCAATTTGGATATTGAGTGTCTTCTGGTTCATGTTCATCACGGCTTAAGCCTCAATGCTGACTCATGG
GCAAAGCACTGCGCTTCTCAGGCTCAGTACTATGAACTCGGCTGCGCTATTAAGCGAGTCGAGGTTAAGCTCGCCCCACG
GGTGAGTGTAGAGGCGCAGGCTCGCACTGTTCGATATCAGGCACTGTTATCTGAGCTTGAGCAAGGTGATGTGTTGATGA
CTGCGCACCATCAAGACGACCAGCTCGAAACCGTTTTGCTGGCATTAAAGCGAGGTCTCGGCCCTAAAGGCTTGGCCGCC
ATGGGCAAGGTGCAGTGCTTTGAACAACAACACTGGCTGATTAGGCCACTGCTAGATTTCAGTAAAGTAGAGATTGAAGT
CTACGCAAAACAGCACAATATTTCCCATATTGAAGATGAAAGTAATTTCGATGATAAGTACGACAGAAACTTTCTTCGCT
TAGAAGTTATTCCAACACTTAAGCAGCGCTGGAGTGCCATTGCGGCAACGGCTAGTCGCAGCGCCGAAATATGTGCACTG
CAGCAATCGGTTATCGATGAAGAGACTGCAGTTAGATTACCGCAACTTCAGCATCACAAAGCCGGGGAGTGCACCTTAGA
TTTAGACAAGCTCGCAACTTATTCTAAGGCCTGGCAGTCCCTGTTGCTGCGTGGCCATATGGAGTCGTTAGGCTTTGCTC
CGCCATCTAAGGTACAAGGTGAGGAGATCTTAACTCAGTTGTTAGAGGCAAAGGCTGATGCGAGTGTTGAAATACGCCTA
GCAGATGTGGTCATCAGGCGCTTTAAACGAGATGGATATTTTTTATCGCTAATTTCAACGCAGCATAAAGCGAAGAAAAC
CTATCCAGCGTTAGCACTTAATAATATTGAAGTGTTATTGAGTGGTGACGTTTTATTGCCAACCGGTAGCCATCTATTTG
CACAAACTTGTACAACTGGCGCTCGACTTAGATTACCAACTGACTTAGAACAGGTTTCCATTGTATTTGCACTGCCTGGT
AGTACGCGATGTCAGCCCCATAACCGTAGCAAAGGTCGCGAGCTAAAGAAGTTGTGGCAAGAACTCGAATTACCCCCTTG
GGAGCGTGGACGAGTGCCTCTGCTGTGTTTTGACGGTAAACTGGTGGCGGCTTGTGGTTATTGGGTAGAGAAATCCTATT
TAGCGAGCAACCAAGCTTTGGGACTCAATCTGAGTCTTAGACACGCTTGA

Upstream 100 bases:

>100_bases
AGCTTATGTTGGCGCTAGAGTCGTTAACAGGTTCAGGCAAAGTTAAGATCGTTTTTTAATACTGTTTTTAAAACTCTTTT
TAAGGTAATGGCTTTCTAAT

Downstream 100 bases:

>100_bases
GTGGGCAGTTAGCCCTGTCTACAATTCTTCATTCATCTGACTGCTTCTAAGCGGCACAAATTTGGTAGCATTGTCTACCT
GCCGCTTTTGCTTCATATAA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MDISASISECMKLLPQRSINTEVTGEQVCQKRRKLVLAYSGGVDSEILAHGLSQFAKANLDIECLLVHVHHGLSLNADSW
AKHCASQAQYYELGCAIKRVEVKLAPRVSVEAQARTVRYQALLSELEQGDVLMTAHHQDDQLETVLLALKRGLGPKGLAA
MGKVQCFEQQHWLIRPLLDFSKVEIEVYAKQHNISHIEDESNFDDKYDRNFLRLEVIPTLKQRWSAIAATASRSAEICAL
QQSVIDEETAVRLPQLQHHKAGECTLDLDKLATYSKAWQSLLLRGHMESLGFAPPSKVQGEEILTQLLEAKADASVEIRL
ADVVIRRFKRDGYFLSLISTQHKAKKTYPALALNNIEVLLSGDVLLPTGSHLFAQTCTTGARLRLPTDLEQVSIVFALPG
STRCQPHNRSKGRELKKLWQELELPPWERGRVPLLCFDGKLVAACGYWVEKSYLASNQALGLNLSLRHA

Sequences:

>Translated_469_residues
MDISASISECMKLLPQRSINTEVTGEQVCQKRRKLVLAYSGGVDSEILAHGLSQFAKANLDIECLLVHVHHGLSLNADSW
AKHCASQAQYYELGCAIKRVEVKLAPRVSVEAQARTVRYQALLSELEQGDVLMTAHHQDDQLETVLLALKRGLGPKGLAA
MGKVQCFEQQHWLIRPLLDFSKVEIEVYAKQHNISHIEDESNFDDKYDRNFLRLEVIPTLKQRWSAIAATASRSAEICAL
QQSVIDEETAVRLPQLQHHKAGECTLDLDKLATYSKAWQSLLLRGHMESLGFAPPSKVQGEEILTQLLEAKADASVEIRL
ADVVIRRFKRDGYFLSLISTQHKAKKTYPALALNNIEVLLSGDVLLPTGSHLFAQTCTTGARLRLPTDLEQVSIVFALPG
STRCQPHNRSKGRELKKLWQELELPPWERGRVPLLCFDGKLVAACGYWVEKSYLASNQALGLNLSLRHA
>Mature_469_residues
MDISASISECMKLLPQRSINTEVTGEQVCQKRRKLVLAYSGGVDSEILAHGLSQFAKANLDIECLLVHVHHGLSLNADSW
AKHCASQAQYYELGCAIKRVEVKLAPRVSVEAQARTVRYQALLSELEQGDVLMTAHHQDDQLETVLLALKRGLGPKGLAA
MGKVQCFEQQHWLIRPLLDFSKVEIEVYAKQHNISHIEDESNFDDKYDRNFLRLEVIPTLKQRWSAIAATASRSAEICAL
QQSVIDEETAVRLPQLQHHKAGECTLDLDKLATYSKAWQSLLLRGHMESLGFAPPSKVQGEEILTQLLEAKADASVEIRL
ADVVIRRFKRDGYFLSLISTQHKAKKTYPALALNNIEVLLSGDVLLPTGSHLFAQTCTTGARLRLPTDLEQVSIVFALPG
STRCQPHNRSKGRELKKLWQELELPPWERGRVPLLCFDGKLVAACGYWVEKSYLASNQALGLNLSLRHA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=426, Percent_Identity=38.4976525821596, Blast_Score=262, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 52408; Mature: 52408

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISASISECMKLLPQRSINTEVTGEQVCQKRRKLVLAYSGGVDSEILAHGLSQFAKANL
CCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHCCC
DIECLLVHVHHGLSLNADSWAKHCASQAQYYELGCAIKRVEVKLAPRVSVEAQARTVRYQ
CEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHH
ALLSELEQGDVLMTAHHQDDQLETVLLALKRGLGPKGLAAMGKVQCFEQQHWLIRPLLDF
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHCC
SKVEIEVYAKQHNISHIEDESNFDDKYDRNFLRLEVIPTLKQRWSAIAATASRSAEICAL
CEEEEEEEEECCCCCCCCCCCCCCHHHCCCEEEEEEEHHHHHHHHHHHHHHCCCHHHHHH
QQSVIDEETAVRLPQLQHHKAGECTLDLDKLATYSKAWQSLLLRGHMESLGFAPPSKVQG
HHHHHCHHHHHCCCCHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
EEILTQLLEAKADASVEIRLADVVIRRFKRDGYFLSLISTQHKAKKTYPALALNNIEVLL
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCEEECCEEEEE
SGDVLLPTGSHLFAQTCTTGARLRLPTDLEQVSIVFALPGSTRCQPHNRSKGRELKKLWQ
ECCEEECCCCHHHHHHHCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHH
ELELPPWERGRVPLLCFDGKLVAACGYWVEKSYLASNQALGLNLSLRHA
HCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHCCCCCEEEEEEECCC
>Mature Secondary Structure
MDISASISECMKLLPQRSINTEVTGEQVCQKRRKLVLAYSGGVDSEILAHGLSQFAKANL
CCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHCCC
DIECLLVHVHHGLSLNADSWAKHCASQAQYYELGCAIKRVEVKLAPRVSVEAQARTVRYQ
CEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHH
ALLSELEQGDVLMTAHHQDDQLETVLLALKRGLGPKGLAAMGKVQCFEQQHWLIRPLLDF
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHCC
SKVEIEVYAKQHNISHIEDESNFDDKYDRNFLRLEVIPTLKQRWSAIAATASRSAEICAL
CEEEEEEEEECCCCCCCCCCCCCCHHHCCCEEEEEEEHHHHHHHHHHHHHHCCCHHHHHH
QQSVIDEETAVRLPQLQHHKAGECTLDLDKLATYSKAWQSLLLRGHMESLGFAPPSKVQG
HHHHHCHHHHHCCCCHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
EEILTQLLEAKADASVEIRLADVVIRRFKRDGYFLSLISTQHKAKKTYPALALNNIEVLL
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCEEECCEEEEE
SGDVLLPTGSHLFAQTCTTGARLRLPTDLEQVSIVFALPGSTRCQPHNRSKGRELKKLWQ
ECCEEECCCCHHHHHHHCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHH
ELELPPWERGRVPLLCFDGKLVAACGYWVEKSYLASNQALGLNLSLRHA
HCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA