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The map label for this gene is kefB [H]

Identifier: 157962686

GI number: 157962686

Start: 3500940

End: 3502889

Strand: Reverse

Name: kefB [H]

Synonym: Spea_2867

Alternate gene names: 157962686

Gene position: 3502889-3500940 (Counterclockwise)

Preceding gene: 157962688

Following gene: 157962685

Centisome position: 67.69

GC content: 47.23

Gene sequence:

>1950_bases
ATGGAACACAGCTTTCTGATCCAAATATTGCTGATGCTGGTCATTGCCATTGTTTCAATTGCCATATTAAGGCGCGCCGA
ATTACCCGCTATTTTGGCGTATCTAGTGACAGGTGTGATAAGCGGTCCAAGTGGTTTTCATTGGTTTACTCAGCATCAGA
TGCAGTCGGTTGCCGAGCTCGGCGTTGTGTTACTGATGTTTAGCCTAGGTCTCGAGTTTTCTTTACCAAGACTTTGGGCG
ATGCGCCGTACTGTATTTGGTCTAGGTAGCGCTCAAGTCGTTGTGACCACCGTTTTGAGCATGGCAATCGCGCTGTTATG
TAATCTTAGCGTAACCGAGTCTGTTATCGTTGGTTCTGCAATCGCACTTTCTTCTACAGCGATTGTGCTTAAACTCCTCA
ATGAACAAGGTTGGCTGAGGCGACGCCATGGTGAGCTCTCCGTAAGTGTGCTGTTATTTCAAGATATTGCCGTTGTTCCC
CTGCTGATTCTTATGCCCATCTTGGCAAGCGATGGTGACGCCTTGATGTGGAGCGATGTGGTTTACGGTTTGCTCAAAGG
GGTTCTCGCCTTTATTGCCTTGATGGCGTTTGGTAAGTGGGCTTTGCCTAAATTATTCGATGAGGTGGCGCGCTCACGCT
CTAATGAGCTATTTGTACTATCGACTTTAGTCGTTGCCTTAGTGACTGGAGCAATAACCCAGTGGCTGGGTTTATCTATG
GCATTGGGCGCCTTTATGGCGGGGATGTTATTGGGGGAGAGTCAGTATCGGCGTCAGCTCGAGGCCGATATTCGCCCGTT
TAGAGATCTATTGATGGGATTGTTTTTCATATCAATAGGCATGCTGTTGGACTTTAGATTAGTCGCGCAGTTTTGGTGGC
AAATCATCTTAATTTTGATTGCTGTTATCTTAGGTAAGTCACTTATTATCTATTCATTACTTAAGCTTGCTAAAGAGTCT
TTTCGCTCTTCGGTGGCAACGGCACTGAGTTTGGCGCAAGTGGGAGAATTCAGTTTTGTGGTGCTGGCGCTGGCAGTCAA
CTATCAGTTATTGGACGCTAAAACCAGTACCATGTTGGTGATGGTTGCGGTTTTATCTATGGCATTAGCGCCTTGGTTGG
TACGCCATAGTGTAGACATTGCCAGAATGCTTCAAGGGCTAAAGCCTAAGGCTGAGAAGAGTGAGCCGCTTAATGTTCAG
GTCGACACTAAGGGAGACCTAGTTCTAATCTTGGGCTATGGTCGCGTCGGCCAGACCATTGCACGATTTATGAAAACAGA
GGCGATCCCCTATTTAGTGTTAGATCTTGACCCCAAAAGGGTTTCTGAGGCGAAAACGGCAGGAGAGCCAGTTTATTTTG
GGGATGTCTGCAAGAGAAGCATCCTTAAGCAGGCCAAGATCCGCGAAGCAAAACTCATCGTGTTGACCTTCAGCAGCGAG
AGAATACTCGAAGAGGTCTTGCCCCTATGCCGGCAGCTTGCTCCAGAAGCTAAAATTCTCGTTCGCACCCGTGATGACTC
AGGAATGGAAGAGTTAGAAAAGGCGGGTGCCAGTCAAGTGATCCCTGAAACACTCGAAGGTAGCTTGATGTTGGTGTCGC
AGGTACTGTATCAGTGTGGTGTGCCCCTGGCACGAATTTTAAAGCGGCTAGAATCCGAGCGGAGAAATCATTATCAATAC
TTACACGGCTTTTTTTCTGGAGCGGAAACTGACTTAACCTTGGAACTATTGCATGCGGTTGCTTTACCAAAAGGCGCTAA
CGCGGTGGGGCAGACCTTATCCCAGATCCCTTGGAGTAAACTCAAGGTGGAGTTAAGAGCGGTAAGGCGAGCTGGTGCAG
AAGTTGAAAATCCAGAATTAGATTGGAAGATAAAGTCCGGTGATATTCTGATCTTGTTAGGCAAGCCAAGACGAATAGAG
AAAGCGGAGATATTTCTTTTGCAAGGTTAG

Upstream 100 bases:

>100_bases
GTCTTTATTTGTCATTCAGTTAATCTCTAGTTAATCGAATGGAAAATATTGGTGTCGATATTTTGACGGATATCGATGGG
CTAAGGAGAACACCGCACGC

Downstream 100 bases:

>100_bases
GTTGCGGCTAGAGAGCAAAGATTGGCTATAGTTGTTTGTTATTAGCTTAAGCAGTCGTAAAAGCAAGGAGTGGTCTAGTG
CATTATCTTTTGTTTATCTT

Product: sodium/hydrogen exchanger

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MEHSFLIQILLMLVIAIVSIAILRRAELPAILAYLVTGVISGPSGFHWFTQHQMQSVAELGVVLLMFSLGLEFSLPRLWA
MRRTVFGLGSAQVVVTTVLSMAIALLCNLSVTESVIVGSAIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDIAVVP
LLILMPILASDGDALMWSDVVYGLLKGVLAFIALMAFGKWALPKLFDEVARSRSNELFVLSTLVVALVTGAITQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFRLVAQFWWQIILILIAVILGKSLIIYSLLKLAKES
FRSSVATALSLAQVGEFSFVVLALAVNYQLLDAKTSTMLVMVAVLSMALAPWLVRHSVDIARMLQGLKPKAEKSEPLNVQ
VDTKGDLVLILGYGRVGQTIARFMKTEAIPYLVLDLDPKRVSEAKTAGEPVYFGDVCKRSILKQAKIREAKLIVLTFSSE
RILEEVLPLCRQLAPEAKILVRTRDDSGMEELEKAGASQVIPETLEGSLMLVSQVLYQCGVPLARILKRLESERRNHYQY
LHGFFSGAETDLTLELLHAVALPKGANAVGQTLSQIPWSKLKVELRAVRRAGAEVENPELDWKIKSGDILILLGKPRRIE
KAEIFLLQG

Sequences:

>Translated_649_residues
MEHSFLIQILLMLVIAIVSIAILRRAELPAILAYLVTGVISGPSGFHWFTQHQMQSVAELGVVLLMFSLGLEFSLPRLWA
MRRTVFGLGSAQVVVTTVLSMAIALLCNLSVTESVIVGSAIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDIAVVP
LLILMPILASDGDALMWSDVVYGLLKGVLAFIALMAFGKWALPKLFDEVARSRSNELFVLSTLVVALVTGAITQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFRLVAQFWWQIILILIAVILGKSLIIYSLLKLAKES
FRSSVATALSLAQVGEFSFVVLALAVNYQLLDAKTSTMLVMVAVLSMALAPWLVRHSVDIARMLQGLKPKAEKSEPLNVQ
VDTKGDLVLILGYGRVGQTIARFMKTEAIPYLVLDLDPKRVSEAKTAGEPVYFGDVCKRSILKQAKIREAKLIVLTFSSE
RILEEVLPLCRQLAPEAKILVRTRDDSGMEELEKAGASQVIPETLEGSLMLVSQVLYQCGVPLARILKRLESERRNHYQY
LHGFFSGAETDLTLELLHAVALPKGANAVGQTLSQIPWSKLKVELRAVRRAGAEVENPELDWKIKSGDILILLGKPRRIE
KAEIFLLQG
>Mature_649_residues
MEHSFLIQILLMLVIAIVSIAILRRAELPAILAYLVTGVISGPSGFHWFTQHQMQSVAELGVVLLMFSLGLEFSLPRLWA
MRRTVFGLGSAQVVVTTVLSMAIALLCNLSVTESVIVGSAIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDIAVVP
LLILMPILASDGDALMWSDVVYGLLKGVLAFIALMAFGKWALPKLFDEVARSRSNELFVLSTLVVALVTGAITQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFRLVAQFWWQIILILIAVILGKSLIIYSLLKLAKES
FRSSVATALSLAQVGEFSFVVLALAVNYQLLDAKTSTMLVMVAVLSMALAPWLVRHSVDIARMLQGLKPKAEKSEPLNVQ
VDTKGDLVLILGYGRVGQTIARFMKTEAIPYLVLDLDPKRVSEAKTAGEPVYFGDVCKRSILKQAKIREAKLIVLTFSSE
RILEEVLPLCRQLAPEAKILVRTRDDSGMEELEKAGASQVIPETLEGSLMLVSQVLYQCGVPLARILKRLESERRNHYQY
LHGFFSGAETDLTLELLHAVALPKGANAVGQTLSQIPWSKLKVELRAVRRAGAEVENPELDWKIKSGDILILLGKPRRIE
KAEIFLLQG

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=405, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1789749, Length=548, Percent_Identity=35.2189781021898, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1786232, Length=560, Percent_Identity=31.0714285714286, Blast_Score=258, Evalue=1e-69,
Organism=Escherichia coli, GI1786685, Length=539, Percent_Identity=30.2411873840445, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6322367, Length=500, Percent_Identity=22.8, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 71623; Mature: 71623

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHSFLIQILLMLVIAIVSIAILRRAELPAILAYLVTGVISGPSGFHWFTQHQMQSVAEL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GVVLLMFSLGLEFSLPRLWAMRRTVFGLGSAQVVVTTVLSMAIALLCNLSVTESVIVGSA
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDIAVVPLLILMPILASDGDALMWSDV
HHHHHHHHHHHHHHCCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VYGLLKGVLAFIALMAFGKWALPKLFDEVARSRSNELFVLSTLVVALVTGAITQWLGLSM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFRLVAQFWWQIILILI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVILGKSLIIYSLLKLAKESFRSSVATALSLAQVGEFSFVVLALAVNYQLLDAKTSTMLV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHH
MVAVLSMALAPWLVRHSVDIARMLQGLKPKAEKSEPLNVQVDTKGDLVLILGYGRVGQTI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEECCHHHHHH
ARFMKTEAIPYLVLDLDPKRVSEAKTAGEPVYFGDVCKRSILKQAKIREAKLIVLTFSSE
HHHHHHCCCCEEEEECCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCHH
RILEEVLPLCRQLAPEAKILVRTRDDSGMEELEKAGASQVIPETLEGSLMLVSQVLYQCG
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHCC
VPLARILKRLESERRNHYQYLHGFFSGAETDLTLELLHAVALPKGANAVGQTLSQIPWSK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCHHH
LKVELRAVRRAGAEVENPELDWKIKSGDILILLGKPRRIEKAEIFLLQG
HHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MEHSFLIQILLMLVIAIVSIAILRRAELPAILAYLVTGVISGPSGFHWFTQHQMQSVAEL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GVVLLMFSLGLEFSLPRLWAMRRTVFGLGSAQVVVTTVLSMAIALLCNLSVTESVIVGSA
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDIAVVPLLILMPILASDGDALMWSDV
HHHHHHHHHHHHHHCCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VYGLLKGVLAFIALMAFGKWALPKLFDEVARSRSNELFVLSTLVVALVTGAITQWLGLSM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFRLVAQFWWQIILILI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVILGKSLIIYSLLKLAKESFRSSVATALSLAQVGEFSFVVLALAVNYQLLDAKTSTMLV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHH
MVAVLSMALAPWLVRHSVDIARMLQGLKPKAEKSEPLNVQVDTKGDLVLILGYGRVGQTI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEECCHHHHHH
ARFMKTEAIPYLVLDLDPKRVSEAKTAGEPVYFGDVCKRSILKQAKIREAKLIVLTFSSE
HHHHHHCCCCEEEEECCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCHH
RILEEVLPLCRQLAPEAKILVRTRDDSGMEELEKAGASQVIPETLEGSLMLVSQVLYQCG
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHCC
VPLARILKRLESERRNHYQYLHGFFSGAETDLTLELLHAVALPKGANAVGQTLSQIPWSK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCHHH
LKVELRAVRRAGAEVENPELDWKIKSGDILILLGKPRRIEKAEIFLLQG
HHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA