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The map label for this gene is pheS [H]

Identifier: 157962291

GI number: 157962291

Start: 3013343

End: 3014329

Strand: Reverse

Name: pheS [H]

Synonym: Spea_2470

Alternate gene names: 157962291

Gene position: 3014329-3013343 (Counterclockwise)

Preceding gene: 157962294

Following gene: 157962290

Centisome position: 58.25

GC content: 44.68

Gene sequence:

>987_bases
ATGATGTCACAGTTAACAGAGATCGTAGAACAGGCTTTAGCCGCCATCGAAGGAGCTGCAGATTTAAAAGCTCTCGATGA
CCTCCGTGTCGATTACCTTGGTAAGAAAGGTCAAATCACCGACATGATGAAAATGATGGGGAAACTTCCTCCAGAAGAGA
AACCTGCTTTTGGTCAAGCCGTGAACCAAGCTAAGCAAGCGGTTCAGCAGCAGCTTTCTATTCGCATTGAAGGTCTGAAA
GCTGCTGAGTTAGAAGCCCAGCTAAAAGCAGAAAGCATCGATGTGAGTCTTCCTGGTCGTCGTATCGAGAACGGTGGTTT
ACACCCAGTTACTCGTACTATTGAACGTATTGAAGCTTTCTTCGGTGAGCTTGGTTTTGTCGTTAAAGACGGACCTGAAA
TCGAAGATGATTTTCATAACTTTGACGCGTTAAACATCTCTGAGCATCATCCAGCTCGTGCCGATCATGATACTTTCTAC
TTTAATCCTAAAGTGATGTTACGTACGCAAACCTCTGGTGTGCAGATCCGTACCATGGAGCACGAGAAGCCACCATTACG
TATTATCTCTCCAGGCCGTGTATATCGTAACGATTACGACCAAACTCATACGCCAATGTTCCATCAGGTGGAAGGTTTAC
TGGTTGCTGAAAACGTAAACTTTGCTGAGCTTAAAGGCATCTTGCACGACTTCCTACTTAATTTCTTTGAAGAAGATTTA
GAAGTGCGTTTCCGTCCATCTTACTTTCCGTTTACTGAGCCTTCAGCCGAAGTTGACGTAATGGGGAAAAACGGTAAGTG
GTTAGAAGTATTGGGTTGCGGCATGGTACATCCAAACGTACTGCGTAGCGTTGGCATCGATCCTGAGAAGTACTCTGGCT
TTGCATTCGGTATGGGCGTTGAGCGTCTTTCAATGTTGCGTTATGGCGTTAACGACTTACGTTCATTCTTCGAAAACGAT
CTACGCTTCCTGAAGCAATTTAAATAA

Upstream 100 bases:

>100_bases
CGCCCCTGTCTAACGTAAAATTTTAGTCTTTTTCTATTTTCACAAAGAAAATACGTTAGCCCAAAAGTCGTAGGTCTAGG
ACTTACTTGCACTGGAAGAG

Downstream 100 bases:

>100_bases
ACGGAGCTGTAATAGCATGAAATTTAGTGAATCTTGGCTTCGTGAGTGGGTTAACCCAAGCGTTAATCGTGAAGAGTTAG
CACACCAAATTACTATGGCC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MMSQLTEIVEQALAAIEGAADLKALDDLRVDYLGKKGQITDMMKMMGKLPPEEKPAFGQAVNQAKQAVQQQLSIRIEGLK
AAELEAQLKAESIDVSLPGRRIENGGLHPVTRTIERIEAFFGELGFVVKDGPEIEDDFHNFDALNISEHHPARADHDTFY
FNPKVMLRTQTSGVQIRTMEHEKPPLRIISPGRVYRNDYDQTHTPMFHQVEGLLVAENVNFAELKGILHDFLLNFFEEDL
EVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLSMLRYGVNDLRSFFEND
LRFLKQFK

Sequences:

>Translated_328_residues
MMSQLTEIVEQALAAIEGAADLKALDDLRVDYLGKKGQITDMMKMMGKLPPEEKPAFGQAVNQAKQAVQQQLSIRIEGLK
AAELEAQLKAESIDVSLPGRRIENGGLHPVTRTIERIEAFFGELGFVVKDGPEIEDDFHNFDALNISEHHPARADHDTFY
FNPKVMLRTQTSGVQIRTMEHEKPPLRIISPGRVYRNDYDQTHTPMFHQVEGLLVAENVNFAELKGILHDFLLNFFEEDL
EVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLSMLRYGVNDLRSFFEND
LRFLKQFK
>Mature_328_residues
MMSQLTEIVEQALAAIEGAADLKALDDLRVDYLGKKGQITDMMKMMGKLPPEEKPAFGQAVNQAKQAVQQQLSIRIEGLK
AAELEAQLKAESIDVSLPGRRIENGGLHPVTRTIERIEAFFGELGFVVKDGPEIEDDFHNFDALNISEHHPARADHDTFY
FNPKVMLRTQTSGVQIRTMEHEKPPLRIISPGRVYRNDYDQTHTPMFHQVEGLLVAENVNFAELKGILHDFLLNFFEEDL
EVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLSMLRYGVNDLRSFFEND
LRFLKQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=274, Percent_Identity=33.2116788321168, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI4758340, Length=259, Percent_Identity=32.046332046332, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=75.2293577981651, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI32566635, Length=243, Percent_Identity=34.5679012345679, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32563657, Length=260, Percent_Identity=30.3846153846154, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=260, Percent_Identity=30.3846153846154, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321087, Length=369, Percent_Identity=27.6422764227642, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=268, Percent_Identity=27.9850746268657, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18858079, Length=284, Percent_Identity=29.9295774647887, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17137424, Length=280, Percent_Identity=28.9285714285714, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 37217; Mature: 37217

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSQLTEIVEQALAAIEGAADLKALDDLRVDYLGKKGQITDMMKMMGKLPPEEKPAFGQA
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHH
VNQAKQAVQQQLSIRIEGLKAAELEAQLKAESIDVSLPGRRIENGGLHPVTRTIERIEAF
HHHHHHHHHHHHHEEECCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
FGELGFVVKDGPEIEDDFHNFDALNISEHHPARADHDTFYFNPKVMLRTQTSGVQIRTME
HHHCCEEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCEEEEEEC
HEKPPLRIISPGRVYRNDYDQTHTPMFHQVEGLLVAENVNFAELKGILHDFLLNFFEEDL
CCCCCEEEECCCHHHCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
EVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGV
CEEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEEHHHH
ERLSMLRYGVNDLRSFFENDLRFLKQFK
HHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMSQLTEIVEQALAAIEGAADLKALDDLRVDYLGKKGQITDMMKMMGKLPPEEKPAFGQA
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHH
VNQAKQAVQQQLSIRIEGLKAAELEAQLKAESIDVSLPGRRIENGGLHPVTRTIERIEAF
HHHHHHHHHHHHHEEECCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
FGELGFVVKDGPEIEDDFHNFDALNISEHHPARADHDTFYFNPKVMLRTQTSGVQIRTME
HHHCCEEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCEEEEEEC
HEKPPLRIISPGRVYRNDYDQTHTPMFHQVEGLLVAENVNFAELKGILHDFLLNFFEEDL
CCCCCEEEECCCHHHCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
EVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGV
CEEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEEHHHH
ERLSMLRYGVNDLRSFFENDLRFLKQFK
HHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA