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The map label for this gene is pheT [H]

Identifier: 157962290

GI number: 157962290

Start: 3010939

End: 3013326

Strand: Reverse

Name: pheT [H]

Synonym: Spea_2469

Alternate gene names: 157962290

Gene position: 3013326-3010939 (Counterclockwise)

Preceding gene: 157962291

Following gene: 157962289

Centisome position: 58.23

GC content: 44.97

Gene sequence:

>2388_bases
ATGAAATTTAGTGAATCTTGGCTTCGTGAGTGGGTTAACCCAAGCGTTAATCGTGAAGAGTTAGCACACCAAATTACTAT
GGCCGGTCTTGAAGTTGACGGCATTGAGCCAGTAGCTGCCGAGTTTAGCGGTGTAGTTGTCGGTGAAGTCGTTGAGTGTG
GCCAGCATCCAGATGCAGACAAATTACGCGTAACTAAAATCAATGTCGGCGGCGAAGAGCTAATCGACATCGTATGTGGT
GCGTCAAACTGTCGTCTAGGTCTTAAGGTTGCAGTCGCTATGGTTGGCGCTGTGCTACCAGGTGATTTCAAAATCAAAAA
AGCCAAACTACGTGGACAGCCATCATTTGGTATGTTGTGCTCATTTGGCGAGCTTGGTGTAGATATTGAAAGTGACGGCA
TTATTGAATTGCCACTTGACGCACCTGTTGGCCAAGACGTACGTGAATTCTTAGATCTAAACGATGCCATTATTGATGTC
GATTTAACGGCTAACCGTGCAGATTGTCTAGGTATGGCAGGTCTTGCTCGTGAAGTAGGCGTGTTAAACCGTCAATCGGT
TACTGAACCGACTTGGGATGCGGTTGAAGCGACAATTGCTGATGCAGTTACCGTTGATATTCAAGAACCTCAAGAGTGTG
GTCGTTACTTAGGCCGCGTTATCAAGAATGTTAACGTTAAGGCTGAATCACCACTATGGATGCAAGAGAAATTGCGTCGT
AGCGGTATCCGCTCTATCGATCCTATCGTCGATATTACTAACTATGTATTGATTGAGTTTGGTCAACCAATGCACGCATT
TGACTTAGCTAAGTTAGATGGCGGTATTGTGGTTCGTAAGCCCAACGGCGAAGAGAAACTGACTCTACTTGATGGCAATG
AAATCACTATTCCTGAAGGTATGTTGATCATTGCTGACCACGCTAAGCCATTGGCTCTAGCGGGTGTATTTGGTGGTGAG
CACAGCGGTGTGACCACAGAAACCACTGATATCGTACTTGAGTGCGCTTACTTTGCACCGCTAGCTATCTTAGGCAAGGC
ACGTAAGATTGGTCTGCATACTGATGCATCACACCGTTTCGAACGTGGTGTTGACCCAGAGCTACAACAGAAGGTTATGG
ATCGTGCTAGTCGTTTAGTGCTAGAGATCTGTGGTGGTGAAGCTGGCCCAGTTGTTGAAGCAAAGTCTGAAGAGAATCTA
CCTAAGCCTGTACAGATCGTATTGCGCCGTAGCAAGCTAGATAAGACTTTGGGTCATCATATTGATGATGCTGAAGTTAA
AGAAATTCTAGAGCGTTTAGGTTTCATCGTCGTAGTGAGTGAAGGGCTGTGGAATGTAACCACGGCAACTTACCGCTTCG
ATATGGCAATAGAAGAAGACTTGATTGAAGAAGTTGCCCGTATTTACGGTTACAACAACATTCCAAATATCGCGCCTATT
GCTCATTTAAGCATGTCTGACCATAAAGAGTCGAACATCAACATTAAGCGTGTTCGTAGCATGCTGGTTGCTCGAGGTTT
CCAAGAAGCTGTGACTTATAGCTTTGTGGATCCGAAGCAGCAAGAGTTGATTCACCCAGGTGCAGAGGCGATGATTCTAC
CGAATCCAATTTCGGTTGAAATGTCTGCTATGCGTCTGTCTATGTTCACCGGTCTTTTGACTGCAGTTGGTTATAACCAA
AGCCGTCAGCAAAACCGTGTCAGACTGTTCGAAACAGGTCTACGTTTTGTACCAAATGAAACAGCAGAGTCTGGTGTGTC
ACAACAACCTATGCTTGCTGCTGTTATTGCCGGTAACCAAAATGACGAGCATTGGACAATGGAGTCAAAAACCGTTGACT
TCTTTGACCTAAAAGGTGACTTAGAAGCAGTCATCGGTTTGACTGTTGCTGATGCTGAATTTAGTTTCAGACCAGCAACT
CATCCAGCGTTACATCCGGGGCAATGTGCTGAAATCTTAAGAGATGATCGCGTTATTGGTATCATAGGTACGGTTCACCC
AAGCCTAGAGAAGCCATTTGGCCTAAACGGAAAAACAATTATTTTCGAGCTAGAATTAGACGCTTTATTGCACGCTAATT
TGCCGCTAGCCCAGACTGTATCTAAGTTTCCAGCTAACCGCCGTGATATCGCCATCGTAGTTGATGACGCTATTGCAGCG
AATGATGTTGTAAAAATGATAAGAAAAGTTGGCGAAAATCAGTTGGTTGGCATAAACTTGTTCGATGTATACCGAGGTAA
AGGTGTAGAGGAAGGCAAAAAGAGCCTTGCAATAGCACTCTTATTACAAGACAACGCTCGTACACTGGAAGAAAAAGAAA
TCGCTGAGATGGTAGACGTCGTGGTTTCTGCTCTGAAAGACGAGTTCAACGCATCGTTGAGGGACTAA

Upstream 100 bases:

>100_bases
CGTCTTTCAATGTTGCGTTATGGCGTTAACGACTTACGTTCATTCTTCGAAAACGATCTACGCTTCCTGAAGCAATTTAA
ATAAACGGAGCTGTAATAGC

Downstream 100 bases:

>100_bases
AAGTATGGCACTTACCAAAGCCGAAATGGCAGAACATCTTTTTGAAACGCTAGGTATCAACAAGAGAGTAGCGAAAGAGA
TGGTTGAGACGTTTTTCGAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSESWLREWVNPSVNREELAHQITMAGLEVDGIEPVAAEFSGVVVGEVVECGQHPDADKLRVTKINVGGEELIDIVCG
ASNCRLGLKVAVAMVGAVLPGDFKIKKAKLRGQPSFGMLCSFGELGVDIESDGIIELPLDAPVGQDVREFLDLNDAIIDV
DLTANRADCLGMAGLAREVGVLNRQSVTEPTWDAVEATIADAVTVDIQEPQECGRYLGRVIKNVNVKAESPLWMQEKLRR
SGIRSIDPIVDITNYVLIEFGQPMHAFDLAKLDGGIVVRKPNGEEKLTLLDGNEITIPEGMLIIADHAKPLALAGVFGGE
HSGVTTETTDIVLECAYFAPLAILGKARKIGLHTDASHRFERGVDPELQQKVMDRASRLVLEICGGEAGPVVEAKSEENL
PKPVQIVLRRSKLDKTLGHHIDDAEVKEILERLGFIVVVSEGLWNVTTATYRFDMAIEEDLIEEVARIYGYNNIPNIAPI
AHLSMSDHKESNINIKRVRSMLVARGFQEAVTYSFVDPKQQELIHPGAEAMILPNPISVEMSAMRLSMFTGLLTAVGYNQ
SRQQNRVRLFETGLRFVPNETAESGVSQQPMLAAVIAGNQNDEHWTMESKTVDFFDLKGDLEAVIGLTVADAEFSFRPAT
HPALHPGQCAEILRDDRVIGIIGTVHPSLEKPFGLNGKTIIFELELDALLHANLPLAQTVSKFPANRRDIAIVVDDAIAA
NDVVKMIRKVGENQLVGINLFDVYRGKGVEEGKKSLAIALLLQDNARTLEEKEIAEMVDVVVSALKDEFNASLRD

Sequences:

>Translated_795_residues
MKFSESWLREWVNPSVNREELAHQITMAGLEVDGIEPVAAEFSGVVVGEVVECGQHPDADKLRVTKINVGGEELIDIVCG
ASNCRLGLKVAVAMVGAVLPGDFKIKKAKLRGQPSFGMLCSFGELGVDIESDGIIELPLDAPVGQDVREFLDLNDAIIDV
DLTANRADCLGMAGLAREVGVLNRQSVTEPTWDAVEATIADAVTVDIQEPQECGRYLGRVIKNVNVKAESPLWMQEKLRR
SGIRSIDPIVDITNYVLIEFGQPMHAFDLAKLDGGIVVRKPNGEEKLTLLDGNEITIPEGMLIIADHAKPLALAGVFGGE
HSGVTTETTDIVLECAYFAPLAILGKARKIGLHTDASHRFERGVDPELQQKVMDRASRLVLEICGGEAGPVVEAKSEENL
PKPVQIVLRRSKLDKTLGHHIDDAEVKEILERLGFIVVVSEGLWNVTTATYRFDMAIEEDLIEEVARIYGYNNIPNIAPI
AHLSMSDHKESNINIKRVRSMLVARGFQEAVTYSFVDPKQQELIHPGAEAMILPNPISVEMSAMRLSMFTGLLTAVGYNQ
SRQQNRVRLFETGLRFVPNETAESGVSQQPMLAAVIAGNQNDEHWTMESKTVDFFDLKGDLEAVIGLTVADAEFSFRPAT
HPALHPGQCAEILRDDRVIGIIGTVHPSLEKPFGLNGKTIIFELELDALLHANLPLAQTVSKFPANRRDIAIVVDDAIAA
NDVVKMIRKVGENQLVGINLFDVYRGKGVEEGKKSLAIALLLQDNARTLEEKEIAEMVDVVVSALKDEFNASLRD
>Mature_795_residues
MKFSESWLREWVNPSVNREELAHQITMAGLEVDGIEPVAAEFSGVVVGEVVECGQHPDADKLRVTKINVGGEELIDIVCG
ASNCRLGLKVAVAMVGAVLPGDFKIKKAKLRGQPSFGMLCSFGELGVDIESDGIIELPLDAPVGQDVREFLDLNDAIIDV
DLTANRADCLGMAGLAREVGVLNRQSVTEPTWDAVEATIADAVTVDIQEPQECGRYLGRVIKNVNVKAESPLWMQEKLRR
SGIRSIDPIVDITNYVLIEFGQPMHAFDLAKLDGGIVVRKPNGEEKLTLLDGNEITIPEGMLIIADHAKPLALAGVFGGE
HSGVTTETTDIVLECAYFAPLAILGKARKIGLHTDASHRFERGVDPELQQKVMDRASRLVLEICGGEAGPVVEAKSEENL
PKPVQIVLRRSKLDKTLGHHIDDAEVKEILERLGFIVVVSEGLWNVTTATYRFDMAIEEDLIEEVARIYGYNNIPNIAPI
AHLSMSDHKESNINIKRVRSMLVARGFQEAVTYSFVDPKQQELIHPGAEAMILPNPISVEMSAMRLSMFTGLLTAVGYNQ
SRQQNRVRLFETGLRFVPNETAESGVSQQPMLAAVIAGNQNDEHWTMESKTVDFFDLKGDLEAVIGLTVADAEFSFRPAT
HPALHPGQCAEILRDDRVIGIIGTVHPSLEKPFGLNGKTIIFELELDALLHANLPLAQTVSKFPANRRDIAIVVDDAIAA
NDVVKMIRKVGENQLVGINLFDVYRGKGVEEGKKSLAIALLLQDNARTLEEKEIAEMVDVVVSALKDEFNASLRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=61.1320754716981, Blast_Score=987, Evalue=0.0,
Organism=Drosophila melanogaster, GI21355211, Length=573, Percent_Identity=23.5602094240838, Blast_Score=80, Evalue=6e-15,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 87016; Mature: 87016

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSESWLREWVNPSVNREELAHQITMAGLEVDGIEPVAAEFSGVVVGEVVECGQHPDAD
CCCCHHHHHHHCCCCCCHHHHHHHHHHCCEEECCCCHHHHHHCCCHHHHHHHCCCCCCCC
KLRVTKINVGGEELIDIVCGASNCRLGLKVAVAMVGAVLPGDFKIKKAKLRGQPSFGMLC
CEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCEE
SFGELGVDIESDGIIELPLDAPVGQDVREFLDLNDAIIDVDLTANRADCLGMAGLAREVG
ECCCCCCEECCCCEEEECCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHC
VLNRQSVTEPTWDAVEATIADAVTVDIQEPQECGRYLGRVIKNVNVKAESPLWMQEKLRR
CCCCCCCCCCCHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
SGIRSIDPIVDITNYVLIEFGQPMHAFDLAKLDGGIVVRKPNGEEKLTLLDGNEITIPEG
CCCCCCCHHHHHHCEEEEECCCCCHHHHHHCCCCCEEEECCCCCCEEEEECCCEEECCCC
MLIIADHAKPLALAGVFGGEHSGVTTETTDIVLECAYFAPLAILGKARKIGLHTDASHRF
EEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHH
ERGVDPELQQKVMDRASRLVLEICGGEAGPVVEAKSEENLPKPVQIVLRRSKLDKTLGHH
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
IDDAEVKEILERLGFIVVVSEGLWNVTTATYRFDMAIEEDLIEEVARIYGYNNIPNIAPI
CCHHHHHHHHHHCCEEEEEECCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCE
AHLSMSDHKESNINIKRVRSMLVARGFQEAVTYSFVDPKQQELIHPGAEAMILPNPISVE
EECCCCCCCCCCCCHHHHHHHHHHCCHHHHHEEECCCCCHHHHCCCCCCEEEECCCCCCH
MSAMRLSMFTGLLTAVGYNQSRQQNRVRLFETGLRFVPNETAESGVSQQPMLAAVIAGNQ
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEEECCC
NDEHWTMESKTVDFFDLKGDLEAVIGLTVADAEFSFRPATHPALHPGQCAEILRDDRVIG
CCCCEEECCCCEEEEECCCCCEEHEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEE
IIGTVHPSLEKPFGLNGKTIIFELELDALLHANLPLAQTVSKFPANRRDIAIVVDDAIAA
EEECCCCCCCCCCCCCCCEEEEEEEEHHHHCCCCCHHHHHHHCCCCCCEEEEEEECCHHH
NDVVKMIRKVGENQLVGINLFDVYRGKGVEEGKKSLAIALLLQDNARTLEEKEIAEMVDV
HHHHHHHHHCCCCCEEEEEEEEHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
VVSALKDEFNASLRD
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MKFSESWLREWVNPSVNREELAHQITMAGLEVDGIEPVAAEFSGVVVGEVVECGQHPDAD
CCCCHHHHHHHCCCCCCHHHHHHHHHHCCEEECCCCHHHHHHCCCHHHHHHHCCCCCCCC
KLRVTKINVGGEELIDIVCGASNCRLGLKVAVAMVGAVLPGDFKIKKAKLRGQPSFGMLC
CEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCEE
SFGELGVDIESDGIIELPLDAPVGQDVREFLDLNDAIIDVDLTANRADCLGMAGLAREVG
ECCCCCCEECCCCEEEECCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHC
VLNRQSVTEPTWDAVEATIADAVTVDIQEPQECGRYLGRVIKNVNVKAESPLWMQEKLRR
CCCCCCCCCCCHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
SGIRSIDPIVDITNYVLIEFGQPMHAFDLAKLDGGIVVRKPNGEEKLTLLDGNEITIPEG
CCCCCCCHHHHHHCEEEEECCCCCHHHHHHCCCCCEEEECCCCCCEEEEECCCEEECCCC
MLIIADHAKPLALAGVFGGEHSGVTTETTDIVLECAYFAPLAILGKARKIGLHTDASHRF
EEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHH
ERGVDPELQQKVMDRASRLVLEICGGEAGPVVEAKSEENLPKPVQIVLRRSKLDKTLGHH
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
IDDAEVKEILERLGFIVVVSEGLWNVTTATYRFDMAIEEDLIEEVARIYGYNNIPNIAPI
CCHHHHHHHHHHCCEEEEEECCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCE
AHLSMSDHKESNINIKRVRSMLVARGFQEAVTYSFVDPKQQELIHPGAEAMILPNPISVE
EECCCCCCCCCCCCHHHHHHHHHHCCHHHHHEEECCCCCHHHHCCCCCCEEEECCCCCCH
MSAMRLSMFTGLLTAVGYNQSRQQNRVRLFETGLRFVPNETAESGVSQQPMLAAVIAGNQ
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEEECCC
NDEHWTMESKTVDFFDLKGDLEAVIGLTVADAEFSFRPATHPALHPGQCAEILRDDRVIG
CCCCEEECCCCEEEEECCCCCEEHEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEE
IIGTVHPSLEKPFGLNGKTIIFELELDALLHANLPLAQTVSKFPANRRDIAIVVDDAIAA
EEECCCCCCCCCCCCCCCEEEEEEEEHHHHCCCCCHHHHHHHCCCCCCEEEEEEECCHHH
NDVVKMIRKVGENQLVGINLFDVYRGKGVEEGKKSLAIALLLQDNARTLEEKEIAEMVDV
HHHHHHHHHCCCCCEEEEEEEEHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
VVSALKDEFNASLRD
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]