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The map label for this gene is thiE [H]

Identifier: 157962203

GI number: 157962203

Start: 2903306

End: 2904898

Strand: Reverse

Name: thiE [H]

Synonym: Spea_2382

Alternate gene names: 157962203

Gene position: 2904898-2903306 (Counterclockwise)

Preceding gene: 157962204

Following gene: 157962202

Centisome position: 56.14

GC content: 47.71

Gene sequence:

>1593_bases
ATGGCAAACGCACATACATTGCCTGTTAGGCTTCCTGTTGTATGGACGATAGCGGGCTCTGATAGTGGCGGCGGTGCAGG
CATTCAAGCGGATCTGGCAACAATGTCAGATCTTGATGCCCACGCCTGCAGCGTTATCACTGCGGTGACAGCACAGAGCT
CTGTAACTGTGGCATTGGTTGAGTCTGTATCAAGTCAAATGTTGAAGTCTCAGCTAGACACCTTAGTTAGCGACCTACCG
CCAGCAGCCATTAAAATTGGCTTGCTGGCCGAGCAGGCTCAAATTGATTATCTAGCCAAATGGTTAGCTAAGCAGCAAGC
TAGTTGGCGTCAATCTGGCATTGTAGTGCCTATTATTTTAGACCCTGTGATGGTGGCTACCTGCGGTGATGCACTGGCAA
ATGGCGGAAGGCTTGATTTTACGCCGTTTAAAGGATTACTCACATTAATCACGCCTAATGTGAGTGAGTTAGCCATTCTG
GCTGCAACAGAGCTTGAGAGTGTTGATGCATGTATCTCGGCAGCCAAACAGCTATCAGAACAGTTAAGCACTTCGGTGCT
AGCCAAAGGTGGCGATCTTGGTCCAGCCTGGTGTCAGCATCAGGCTAATGATTTACTCGTTTGTACTGATGTTGAAGGAA
TATCTGATAAGCATCAGTGGCAGAGTTTTTGGTTATCAAGTCATCGAGTCAATAGCCAAAATAGTCATGGTACAGGCTGC
ACCTTGTCATCGGCAATTGCATCTGTAATGGCCCATGGCTTTGTGCTCAATGATGCGATTGTTGTGGCAAAGGCCTATGT
GAATGCAGGACTGATCGCCAGCTATCAGCCGGGCAGTGGACCTGGATGCTTGGCAAGAACGGCATGGCCAAAAGACTTAC
AGCAATTTCCCTATATCAAAGTTATCACAGCAAGCGATACCGAACCTATGTCGCTTGCTGCTTGGGAGTTGATGAATAAT
CAGCGAGGCGACTTTAAGCAGATTGATGGCACCTTGGGATTATATCCTGTAGTTGCTGATGTTGAATTGCTACAAATCTT
GTTAAAAGCGGGGGCTAAAACAATTCAGCTGCGAGTTAAGGATGCAAGTTCGCCAACCCTTGAAGCGCAGATAATCAAAG
CCATTGCTCTAGGTCGTGACTATCAATCACGAGTGTTCATTAACGATCACTGGCAGCTTGCCATTAAACATCAAGCTTAC
GGGGTTCACCTTGGTCAGGAAGACTTGCTCGACAGTAATTTAAAGCAGCTACGTGAAGCTGACATTGCACTTGGGTTGTC
GAGTCATAGCTATTTCGAAATTTTGTTGGCAAAGCAACATCAGCCATCATATATCGCATTTGGGCATATATTTCCCACCA
CCACCAAGCAGATGCCATCTTTGCCTCAGGGGCTAGAAAAACTGAGCCGCTATGTTTCTTTGATGAAAGGCGAGCTGCCA
ATCGTCGCAATTGGCGGCATAGATGCAGCGCGCCTTAGAAGTGTTAAAGCAACGGGTGTGGATGACATCGCAGTGGTGCG
AGCTTTGACTGAGGCCGATGATCCCACTGCGGCGTTTACGGCTCTGAATAGGGCATGGGCTGATGAGAAATAG

Upstream 100 bases:

>100_bases
AAGGCATGGCGCAAATGTCGGCCGAGTTTAAAGCTAAAGGTGCTGAACTTTATCATGAAGCGAGTCATAAACAGCCTGAT
GTTGCGTTAGAGGATTAATC

Downstream 100 bases:

>100_bases
TATGTCACTCACTGATAGTGATTTTTTACGATACTCGCGTCAGGTGTTATTGCCTGAAGTTGGCGAGGCTGGTCAGATTG
CCATTTCCAAGTCTCATGTT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MANAHTLPVRLPVVWTIAGSDSGGGAGIQADLATMSDLDAHACSVITAVTAQSSVTVALVESVSSQMLKSQLDTLVSDLP
PAAIKIGLLAEQAQIDYLAKWLAKQQASWRQSGIVVPIILDPVMVATCGDALANGGRLDFTPFKGLLTLITPNVSELAIL
AATELESVDACISAAKQLSEQLSTSVLAKGGDLGPAWCQHQANDLLVCTDVEGISDKHQWQSFWLSSHRVNSQNSHGTGC
TLSSAIASVMAHGFVLNDAIVVAKAYVNAGLIASYQPGSGPGCLARTAWPKDLQQFPYIKVITASDTEPMSLAAWELMNN
QRGDFKQIDGTLGLYPVVADVELLQILLKAGAKTIQLRVKDASSPTLEAQIIKAIALGRDYQSRVFINDHWQLAIKHQAY
GVHLGQEDLLDSNLKQLREADIALGLSSHSYFEILLAKQHQPSYIAFGHIFPTTTKQMPSLPQGLEKLSRYVSLMKGELP
IVAIGGIDAARLRSVKATGVDDIAVVRALTEADDPTAAFTALNRAWADEK

Sequences:

>Translated_530_residues
MANAHTLPVRLPVVWTIAGSDSGGGAGIQADLATMSDLDAHACSVITAVTAQSSVTVALVESVSSQMLKSQLDTLVSDLP
PAAIKIGLLAEQAQIDYLAKWLAKQQASWRQSGIVVPIILDPVMVATCGDALANGGRLDFTPFKGLLTLITPNVSELAIL
AATELESVDACISAAKQLSEQLSTSVLAKGGDLGPAWCQHQANDLLVCTDVEGISDKHQWQSFWLSSHRVNSQNSHGTGC
TLSSAIASVMAHGFVLNDAIVVAKAYVNAGLIASYQPGSGPGCLARTAWPKDLQQFPYIKVITASDTEPMSLAAWELMNN
QRGDFKQIDGTLGLYPVVADVELLQILLKAGAKTIQLRVKDASSPTLEAQIIKAIALGRDYQSRVFINDHWQLAIKHQAY
GVHLGQEDLLDSNLKQLREADIALGLSSHSYFEILLAKQHQPSYIAFGHIFPTTTKQMPSLPQGLEKLSRYVSLMKGELP
IVAIGGIDAARLRSVKATGVDDIAVVRALTEADDPTAAFTALNRAWADEK
>Mature_529_residues
ANAHTLPVRLPVVWTIAGSDSGGGAGIQADLATMSDLDAHACSVITAVTAQSSVTVALVESVSSQMLKSQLDTLVSDLPP
AAIKIGLLAEQAQIDYLAKWLAKQQASWRQSGIVVPIILDPVMVATCGDALANGGRLDFTPFKGLLTLITPNVSELAILA
ATELESVDACISAAKQLSEQLSTSVLAKGGDLGPAWCQHQANDLLVCTDVEGISDKHQWQSFWLSSHRVNSQNSHGTGCT
LSSAIASVMAHGFVLNDAIVVAKAYVNAGLIASYQPGSGPGCLARTAWPKDLQQFPYIKVITASDTEPMSLAAWELMNNQ
RGDFKQIDGTLGLYPVVADVELLQILLKAGAKTIQLRVKDASSPTLEAQIIKAIALGRDYQSRVFINDHWQLAIKHQAYG
VHLGQEDLLDSNLKQLREADIALGLSSHSYFEILLAKQHQPSYIAFGHIFPTTTKQMPSLPQGLEKLSRYVSLMKGELPI
VAIGGIDAARLRSVKATGVDDIAVVRALTEADDPTAAFTALNRAWADEK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=191, Percent_Identity=56.020942408377, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1788420, Length=259, Percent_Identity=34.3629343629344, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325378, Length=268, Percent_Identity=31.7164179104478, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324997, Length=267, Percent_Identity=32.2097378277154, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324517, Length=267, Percent_Identity=32.2097378277154, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 56600; Mature: 56469

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANAHTLPVRLPVVWTIAGSDSGGGAGIQADLATMSDLDAHACSVITAVTAQSSVTVALV
CCCCCEEEEEEEEEEEEECCCCCCCCCCEEHHHHHHCCCHHHHHHHHHHHCCCCCHHHHH
ESVSSQMLKSQLDTLVSDLPPAAIKIGLLAEQAQIDYLAKWLAKQQASWRQSGIVVPIIL
HHHHHHHHHHHHHHHHHHCCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
DPVMVATCGDALANGGRLDFTPFKGLLTLITPNVSELAILAATELESVDACISAAKQLSE
CCHHHHHHHHHHCCCCCEECCHHHCCHHHCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHH
QLSTSVLAKGGDLGPAWCQHQANDLLVCTDVEGISDKHQWQSFWLSSHRVNSQNSHGTGC
HHHHHHHHCCCCCCHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
TLSSAIASVMAHGFVLNDAIVVAKAYVNAGLIASYQPGSGPGCLARTAWPKDLQQFPYIK
CHHHHHHHHHHCCCHHCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHCCEEE
VITASDTEPMSLAAWELMNNQRGDFKQIDGTLGLYPVVADVELLQILLKAGAKTIQLRVK
EEECCCCCCCHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEE
DASSPTLEAQIIKAIALGRDYQSRVFINDHWQLAIKHQAYGVHLGQEDLLDSNLKQLREA
CCCCCCHHHHHHHHHHHCCCCCCEEEEECCEEEEEEEEEEEEECCHHHHHHHHHHHHHHH
DIALGLSSHSYFEILLAKQHQPSYIAFGHIFPTTTKQMPSLPQGLEKLSRYVSLMKGELP
HHEEECCCCCEEEEEEECCCCCCEEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCC
IVAIGGIDAARLRSVKATGVDDIAVVRALTEADDPTAAFTALNRAWADEK
EEEECCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ANAHTLPVRLPVVWTIAGSDSGGGAGIQADLATMSDLDAHACSVITAVTAQSSVTVALV
CCCCEEEEEEEEEEEEECCCCCCCCCCEEHHHHHHCCCHHHHHHHHHHHCCCCCHHHHH
ESVSSQMLKSQLDTLVSDLPPAAIKIGLLAEQAQIDYLAKWLAKQQASWRQSGIVVPIIL
HHHHHHHHHHHHHHHHHHCCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
DPVMVATCGDALANGGRLDFTPFKGLLTLITPNVSELAILAATELESVDACISAAKQLSE
CCHHHHHHHHHHCCCCCEECCHHHCCHHHCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHH
QLSTSVLAKGGDLGPAWCQHQANDLLVCTDVEGISDKHQWQSFWLSSHRVNSQNSHGTGC
HHHHHHHHCCCCCCHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
TLSSAIASVMAHGFVLNDAIVVAKAYVNAGLIASYQPGSGPGCLARTAWPKDLQQFPYIK
CHHHHHHHHHHCCCHHCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHCCEEE
VITASDTEPMSLAAWELMNNQRGDFKQIDGTLGLYPVVADVELLQILLKAGAKTIQLRVK
EEECCCCCCCHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEE
DASSPTLEAQIIKAIALGRDYQSRVFINDHWQLAIKHQAYGVHLGQEDLLDSNLKQLREA
CCCCCCHHHHHHHHHHHCCCCCCEEEEECCEEEEEEEEEEEEECCHHHHHHHHHHHHHHH
DIALGLSSHSYFEILLAKQHQPSYIAFGHIFPTTTKQMPSLPQGLEKLSRYVSLMKGELP
HHEEECCCCCEEEEEEECCCCCCEEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCC
IVAIGGIDAARLRSVKATGVDDIAVVRALTEADDPTAAFTALNRAWADEK
EEEECCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA