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The map label for this gene is thiC

Identifier: 157962204

GI number: 157962204

Start: 2904901

End: 2906904

Strand: Reverse

Name: thiC

Synonym: Spea_2383

Alternate gene names: 157962204

Gene position: 2906904-2904901 (Counterclockwise)

Preceding gene: 157962205

Following gene: 157962203

Centisome position: 56.18

GC content: 47.75

Gene sequence:

>2004_bases
ATGTCAAATCGCCGCGAAACCCGTGCTCAAGCACAACAGTTTATTGATAACCTAAAACCGCTACAGCACCCAAATTCGGA
GAAAGTTTATCTTGAAGGCAGTCGCCCAGATCTTAAAGTGGGCATGCGTCAAATCCATCAAAGCGACACCTTAGTGGCTG
GTGATGAAGCCAACCCCGTATTTGAAGCTAACCCGCCGCTAAAAGTATACGACTGCGCGGGCGCTTACTCAGATCCTGAT
GCAAATATTGATGTACGCAAAGGTCTTGAAAAATTCCGTGAAAACTGGATTGAAGAGCGTGGTGATACTGTGCAGCTTAC
AAGTGCCAGCTCAGGTTTTACCCAGCAGCGCCTCGCAGATGATGGCTTAGATCATCTAAGGTTCGAGTCGTTGCTGCCAC
CACGCCGTGCAAAAGAAGGCAAACGTGTCACCCAATTGCATTATGCCCGCCAAGGCATTATTACCCCTGAGATGGAATAT
ATTGCCATACGCGAGAATATGGCCAAGTCTGAGGTAACCGACCCTATTCTGACCCAAAAAGATAAAGGTGAGAGCTTTGG
CGCGGTGATTGGCGAGCCAATTACCGCTGAATTTGTCAGACAAGAAATTGCTCGTGGCCGCGCAATTATTCCTCTAAACA
TCAACCACCCTGAAGCTGAGCCAATGATTATTGGTCGTAACTTCTTAGTTAAAGTGAATGCCAATATTGGTAATTCGGCG
GTGACGTCATCCATCGAGGAAGAAGTCGAGAAACTGGTTTGGTCGACCCGATGGGGCGCCGATACCGTGATGGACTTATC
TACTGGGCGATATATCCACGAAACTCGTGAATGGATTATTCGTAACTCTCCTGTACCCATTGGTACCGTGCCTATTTATC
AAGCACTAGAGAAGGTTAATGGTGTTGCCGAAGATCTTAACTGGGAAACGTTCCGCGATACCTTAATCGAACAAGCTGAG
CAGGGCGTTGATTACTTCACCATTCATGCCGGTGTATTACTTCGCTATGTGCCGATGACCGCTAAGCGCCTAACAGGCAT
TGTTTCTCGTGGTGGCTCTATCATGGCGAAATGGTGTCTATCGCATCATAAAGAAAATTTCCTTTATGAGCATTTTAGAG
AAATTTGTGAGATCTGCGCCGCCTATGATGTGTCACTTTCATTAGGCGACGGCATGCGTCCAGGCTCGATTGCCGATGCC
AACGATGAAGCCCAGTTCTCTGAGCTAGAAACGCTTGGCAAGCTCGTTAAGGTTGCTTGGGAATATGACGTACAAACTAT
CATCGAAGGCCCTGGGCACATTCCAATGAACTTGATTAAAGAGAACATGGATAAGCAGCTTGAGGTGTGTGATGAAGCGC
CGTTTTATACCTTAGGCCCACAAACCACGGATATTGCTCCTGGGTATGACCATTTCACATCGGGGATCGGCGCGGCAATG
ATCGCCTGGTATGGCTGTGCAATGCTGTGTTATGTCACGCCTAAAGAGCATTTAGGTCTGCCTAACAAGGATGATGTGAA
ACAAGGGCTAATCGCTTATAAGATTGCCGCTCATGCCGGTGATGTGGCAAAAGGGCATCCGAGCGCCCAAATACGTGACA
ACGCATTATCAAAAGCGCGTTTCGAGTTTCGCTGGGAAGATCAATATAACTTAGGGTTAGATCCTGAGACGGCGCGCGCA
TATCACGATGAGTCTTTACCTCAAGAGTCTGCCAAGGTCGCTCATTTCTGCTCTATGTGTGGCCCTAAGTTCTGCTCAAT
GAAGATTACCCAAGAGGTGCGTGATTATGCCGCAGCTCAGGAGCGTGAGGCTGAGGCTAGAGTGATTGATATCAAATCTG
CTACAGAGTATGACTCATACACTGGCAGTGAGTCTGATACGGCAAAACGTGCATCGCAAAGAGAGCAAGGCATGGCGCAA
ATGTCGGCCGAGTTTAAAGCTAAAGGTGCTGAACTTTATCATGAAGCGAGTCATAAACAGCCTGATGTTGCGTTAGAGGA
TTAA

Upstream 100 bases:

>100_bases
ACGTGTAACTAGAATTTACCGCTAGTTATATGTTCAACTTTTCACATTCTCATTTTCTCCTGGCAAGCAACTTCATTTCT
TATAAAAAGTGAGTTTGCTA

Downstream 100 bases:

>100_bases
TCATGGCAAACGCACATACATTGCCTGTTAGGCTTCCTGTTGTATGGACGATAGCGGGCTCTGATAGTGGCGGCGGTGCA
GGCATTCAAGCGGATCTGGC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 667; Mature: 666

Protein sequence:

>667_residues
MSNRRETRAQAQQFIDNLKPLQHPNSEKVYLEGSRPDLKVGMRQIHQSDTLVAGDEANPVFEANPPLKVYDCAGAYSDPD
ANIDVRKGLEKFRENWIEERGDTVQLTSASSGFTQQRLADDGLDHLRFESLLPPRRAKEGKRVTQLHYARQGIITPEMEY
IAIRENMAKSEVTDPILTQKDKGESFGAVIGEPITAEFVRQEIARGRAIIPLNINHPEAEPMIIGRNFLVKVNANIGNSA
VTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIIRNSPVPIGTVPIYQALEKVNGVAEDLNWETFRDTLIEQAE
QGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHKENFLYEHFREICEICAAYDVSLSLGDGMRPGSIADA
NDEAQFSELETLGKLVKVAWEYDVQTIIEGPGHIPMNLIKENMDKQLEVCDEAPFYTLGPQTTDIAPGYDHFTSGIGAAM
IAWYGCAMLCYVTPKEHLGLPNKDDVKQGLIAYKIAAHAGDVAKGHPSAQIRDNALSKARFEFRWEDQYNLGLDPETARA
YHDESLPQESAKVAHFCSMCGPKFCSMKITQEVRDYAAAQEREAEARVIDIKSATEYDSYTGSESDTAKRASQREQGMAQ
MSAEFKAKGAELYHEASHKQPDVALED

Sequences:

>Translated_667_residues
MSNRRETRAQAQQFIDNLKPLQHPNSEKVYLEGSRPDLKVGMRQIHQSDTLVAGDEANPVFEANPPLKVYDCAGAYSDPD
ANIDVRKGLEKFRENWIEERGDTVQLTSASSGFTQQRLADDGLDHLRFESLLPPRRAKEGKRVTQLHYARQGIITPEMEY
IAIRENMAKSEVTDPILTQKDKGESFGAVIGEPITAEFVRQEIARGRAIIPLNINHPEAEPMIIGRNFLVKVNANIGNSA
VTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIIRNSPVPIGTVPIYQALEKVNGVAEDLNWETFRDTLIEQAE
QGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHKENFLYEHFREICEICAAYDVSLSLGDGMRPGSIADA
NDEAQFSELETLGKLVKVAWEYDVQTIIEGPGHIPMNLIKENMDKQLEVCDEAPFYTLGPQTTDIAPGYDHFTSGIGAAM
IAWYGCAMLCYVTPKEHLGLPNKDDVKQGLIAYKIAAHAGDVAKGHPSAQIRDNALSKARFEFRWEDQYNLGLDPETARA
YHDESLPQESAKVAHFCSMCGPKFCSMKITQEVRDYAAAQEREAEARVIDIKSATEYDSYTGSESDTAKRASQREQGMAQ
MSAEFKAKGAELYHEASHKQPDVALED
>Mature_666_residues
SNRRETRAQAQQFIDNLKPLQHPNSEKVYLEGSRPDLKVGMRQIHQSDTLVAGDEANPVFEANPPLKVYDCAGAYSDPDA
NIDVRKGLEKFRENWIEERGDTVQLTSASSGFTQQRLADDGLDHLRFESLLPPRRAKEGKRVTQLHYARQGIITPEMEYI
AIRENMAKSEVTDPILTQKDKGESFGAVIGEPITAEFVRQEIARGRAIIPLNINHPEAEPMIIGRNFLVKVNANIGNSAV
TSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIIRNSPVPIGTVPIYQALEKVNGVAEDLNWETFRDTLIEQAEQ
GVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHKENFLYEHFREICEICAAYDVSLSLGDGMRPGSIADAN
DEAQFSELETLGKLVKVAWEYDVQTIIEGPGHIPMNLIKENMDKQLEVCDEAPFYTLGPQTTDIAPGYDHFTSGIGAAMI
AWYGCAMLCYVTPKEHLGLPNKDDVKQGLIAYKIAAHAGDVAKGHPSAQIRDNALSKARFEFRWEDQYNLGLDPETARAY
HDESLPQESAKVAHFCSMCGPKFCSMKITQEVRDYAAAQEREAEARVIDIKSATEYDSYTGSESDTAKRASQREQGMAQM
SAEFKAKGAELYHEASHKQPDVALED

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=651, Percent_Identity=72.5038402457757, Blast_Score=1002, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_SHEPA (A8H566)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001502238.1
- STRING:   A8H566
- GeneID:   5662776
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_2383
- NMPDR:   fig|398579.3.peg.2050
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   SPEA398579:SPEA_2383-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 74608; Mature: 74476

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: BINDING 235-235 BINDING 264-264 BINDING 293-293 BINDING 329-329 BINDING 429-429 BINDING 456-456

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRRETRAQAQQFIDNLKPLQHPNSEKVYLEGSRPDLKVGMRQIHQSDTLVAGDEANPV
CCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCCCCC
FEANPPLKVYDCAGAYSDPDANIDVRKGLEKFRENWIEERGDTVQLTSASSGFTQQRLAD
EECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHH
DGLDHLRFESLLPPRRAKEGKRVTQLHYARQGIITPEMEYIAIRENMAKSEVTDPILTQK
CCHHHHHHHHCCCCHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHCCC
DKGESFGAVIGEPITAEFVRQEIARGRAIIPLNINHPEAEPMIIGRNFLVKVNANIGNSA
CCCCCCCHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCEEEEEEECCCCCHH
VTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIIRNSPVPIGTVPIYQALEKVN
HHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GVAEDLNWETFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCL
HHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SHHKENFLYEHFREICEICAAYDVSLSLGDGMRPGSIADANDEAQFSELETLGKLVKVAW
HHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EYDVQTIIEGPGHIPMNLIKENMDKQLEVCDEAPFYTLGPQTTDIAPGYDHFTSGIGAAM
HHCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
IAWYGCAMLCYVTPKEHLGLPNKDDVKQGLIAYKIAAHAGDVAKGHPSAQIRDNALSKAR
HHHHHHHHHEEECCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCE
FEFRWEDQYNLGLDPETARAYHDESLPQESAKVAHFCSMCGPKFCSMKITQEVRDYAAAQ
EEEEECCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EREAEARVIDIKSATEYDSYTGSESDTAKRASQREQGMAQMSAEFKAKGAELYHEASHKQ
HHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC
PDVALED
CCCCCCC
>Mature Secondary Structure 
SNRRETRAQAQQFIDNLKPLQHPNSEKVYLEGSRPDLKVGMRQIHQSDTLVAGDEANPV
CCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCCCCC
FEANPPLKVYDCAGAYSDPDANIDVRKGLEKFRENWIEERGDTVQLTSASSGFTQQRLAD
EECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHH
DGLDHLRFESLLPPRRAKEGKRVTQLHYARQGIITPEMEYIAIRENMAKSEVTDPILTQK
CCHHHHHHHHCCCCHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHCCC
DKGESFGAVIGEPITAEFVRQEIARGRAIIPLNINHPEAEPMIIGRNFLVKVNANIGNSA
CCCCCCCHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCEEEEEEECCCCCHH
VTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIIRNSPVPIGTVPIYQALEKVN
HHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GVAEDLNWETFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCL
HHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SHHKENFLYEHFREICEICAAYDVSLSLGDGMRPGSIADANDEAQFSELETLGKLVKVAW
HHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EYDVQTIIEGPGHIPMNLIKENMDKQLEVCDEAPFYTLGPQTTDIAPGYDHFTSGIGAAM
HHCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
IAWYGCAMLCYVTPKEHLGLPNKDDVKQGLIAYKIAAHAGDVAKGHPSAQIRDNALSKAR
HHHHHHHHHEEECCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCE
FEFRWEDQYNLGLDPETARAYHDESLPQESAKVAHFCSMCGPKFCSMKITQEVRDYAAAQ
EEEEECCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EREAEARVIDIKSATEYDSYTGSESDTAKRASQREQGMAQMSAEFKAKGAELYHEASHKQ
HHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC
PDVALED
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA