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The map label for this gene is thiG [H]

Identifier: 157962200

GI number: 157962200

Start: 2901488

End: 2902252

Strand: Reverse

Name: thiG [H]

Synonym: Spea_2379

Alternate gene names: 157962200

Gene position: 2902252-2901488 (Counterclockwise)

Preceding gene: 157962201

Following gene: 157962199

Centisome position: 56.09

GC content: 51.24

Gene sequence:

>765_bases
ATGTTAACGATTGCCGATCACCAATTTGAATCGCGACTATTTACTGGCACAGGAAAATTCGCAACAGCCCAAACCATGTT
GGATGCGGTAGGGGCTTCTGAGTCTCAATTAGTGACTCTAGCGGTTAAAAGGCTAGATCTTAATACTGGCAGTGACAATA
TTCTTAAGCCATTAATCGATAGCGGGGTAAAGCTACTGCCGAACACCGCTGGGGCGCGGAACGTTAAAGAAGCGCTGTTT
GCCGCGCATCTTTCGCGAGAAATGCTGGGGACCAACTGGATAAAGCTTGAGATCCACCCGGATCCCAAATACCTGATGCC
CGATCCCATTGAAACCTTGGAGGCGGCGCGTATTTTATGCGAACAGGGCTATGTGGTCTTGCCCTATGTTCATGCCGATC
CTGTGCTGTGCCGCAGGCTCGAAGAGGTCGGCTGTGCAGCGGTAATGCCGCTTGGCAGTCCTATTGGCTCAAATCAGGGA
CTGGCAAGCGAACCTTTCTTAAAGATCATTATCGAGCAGGCCAAGGTGCCTGTGATAGTCGATGCGGGAATTGGCGCACC
TTCTCAGGCAACACATGCGATGGAGCTCGGCGCCGATGCCGTACTGGTTAATACCGCCATAGCTAGTAGTCGCGATCCGG
TCACTATGGGGCGCTGTTTTGCTCAAGCTGTTAATACTGGACGACAAGCTTATATCGCAGGTTTAGGCGCCGTGAGTCAT
CAAGCGCACAATACCAGTCCGTTAACGGGGTTCTTAAATGATTAG

Upstream 100 bases:

>100_bases
GCGCTAAATGGTGAAGTCTTGCCTCGTGTTCGCTGGCAGACAGTGTCTTGTCTGGCGGATGATAAGCTTGAGATTTTTTC
AGTAGTTGCGGGAGGCTAAA

Downstream 100 bases:

>100_bases
CACTTATTTTGTTGTTAAGGAGTGCTTATGAGCTTTGTTGATGTGTTTAAAAAGCTGTCTCGTTCAGAGCTTAAACTCAG
ATTGTATTCAAGCACAGCTG

Product: thiazole biosynthesis family protein

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLTIADHQFESRLFTGTGKFATAQTMLDAVGASESQLVTLAVKRLDLNTGSDNILKPLIDSGVKLLPNTAGARNVKEALF
AAHLSREMLGTNWIKLEIHPDPKYLMPDPIETLEAARILCEQGYVVLPYVHADPVLCRRLEEVGCAAVMPLGSPIGSNQG
LASEPFLKIIIEQAKVPVIVDAGIGAPSQATHAMELGADAVLVNTAIASSRDPVTMGRCFAQAVNTGRQAYIAGLGAVSH
QAHNTSPLTGFLND

Sequences:

>Translated_254_residues
MLTIADHQFESRLFTGTGKFATAQTMLDAVGASESQLVTLAVKRLDLNTGSDNILKPLIDSGVKLLPNTAGARNVKEALF
AAHLSREMLGTNWIKLEIHPDPKYLMPDPIETLEAARILCEQGYVVLPYVHADPVLCRRLEEVGCAAVMPLGSPIGSNQG
LASEPFLKIIIEQAKVPVIVDAGIGAPSQATHAMELGADAVLVNTAIASSRDPVTMGRCFAQAVNTGRQAYIAGLGAVSH
QAHNTSPLTGFLND
>Mature_254_residues
MLTIADHQFESRLFTGTGKFATAQTMLDAVGASESQLVTLAVKRLDLNTGSDNILKPLIDSGVKLLPNTAGARNVKEALF
AAHLSREMLGTNWIKLEIHPDPKYLMPDPIETLEAARILCEQGYVVLPYVHADPVLCRRLEEVGCAAVMPLGSPIGSNQG
LASEPFLKIIIEQAKVPVIVDAGIGAPSQATHAMELGADAVLVNTAIASSRDPVTMGRCFAQAVNTGRQAYIAGLGAVSH
QAHNTSPLTGFLND

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=66.403162055336, Blast_Score=344, Evalue=4e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 26947; Mature: 26947

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIADHQFESRLFTGTGKFATAQTMLDAVGASESQLVTLAVKRLDLNTGSDNILKPLID
CCEECCCHHHCEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHCCCCCCCHHHHHHHH
SGVKLLPNTAGARNVKEALFAAHLSREMLGTNWIKLEIHPDPKYLMPDPIETLEAARILC
CCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
EQGYVVLPYVHADPVLCRRLEEVGCAAVMPLGSPIGSNQGLASEPFLKIIIEQAKVPVIV
HCCCEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEE
DAGIGAPSQATHAMELGADAVLVNTAIASSRDPVTMGRCFAQAVNTGRQAYIAGLGAVSH
ECCCCCCCHHHHHHHHCCCEEEEEHHHHCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHH
QAHNTSPLTGFLND
CCCCCCCCEECCCC
>Mature Secondary Structure
MLTIADHQFESRLFTGTGKFATAQTMLDAVGASESQLVTLAVKRLDLNTGSDNILKPLID
CCEECCCHHHCEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHCCCCCCCHHHHHHHH
SGVKLLPNTAGARNVKEALFAAHLSREMLGTNWIKLEIHPDPKYLMPDPIETLEAARILC
CCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
EQGYVVLPYVHADPVLCRRLEEVGCAAVMPLGSPIGSNQGLASEPFLKIIIEQAKVPVIV
HCCCEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEE
DAGIGAPSQATHAMELGADAVLVNTAIASSRDPVTMGRCFAQAVNTGRQAYIAGLGAVSH
ECCCCCCCHHHHHHHHCCCEEEEEHHHHCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHH
QAHNTSPLTGFLND
CCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA