Click here to switch to the map view.

The map label for this gene is aspS [H]

Identifier: 157962187

GI number: 157962187

Start: 2883590

End: 2885383

Strand: Reverse

Name: aspS [H]

Synonym: Spea_2366

Alternate gene names: 157962187

Gene position: 2885383-2883590 (Counterclockwise)

Preceding gene: 157962188

Following gene: 157962186

Centisome position: 55.76

GC content: 45.99

Gene sequence:

>1794_bases
ATGCGCAGTCATTATTGTGGAGACGTCAATAAGTCTCACGTAGGTCAAGAAGTGACCCTAGTGGGTTGGGTTAATCGCAG
CCGAGATTTGGGCGGCGTAGTTTTTCTCGATCTTCGTGATAGAGAAGGTATCGTTCAGGTTGTTTATGATCCAGACCTTC
CTGAAGTTTTTGATGTTGCAAGCACACTACGCAGCGAATTTTGTGTTCAGATTAAAGGCCTTGTAAGAGCGAGACCGGAT
AGCCAGATTAACGCAGATATGCGTACTGGTGAGGTTGAGATCTTGGGTCTAGAGCTAACTATTTTAAACAGCTCAGCGCC
ATTGCCAATCAACATGGACAAAAATCAGCATAACACTGAAGAGCAGCGTTTAAAGTATCGTTACTTAGACCTACGTCGTC
CAGAAATGGCTGACCGTATCGTATTCCGTTCAAAGGTTACTAGCGCGGTAAGACGTTTCTTAGATGGCAACGGTTTCTTA
GATATTGAAACCCCAATCCTGACTAAAGCGACACCAGAAGGTGCGCGTGATTACCTAGTACCAAGCCGTACTTATAAAGG
TCAGTTCTTCGCATTACCGCAATCACCACAATTGTTTAAGCAGTTGTTGATGATGTCAGGTTTTGACCGTTACTACCAAA
TCGTTAAGTGCTTCCGAGATGAAGACTTACGTGCTGACCGCCAACCAGAATTCACTCAGATCGATATCGAAACTTCATTC
ATGACTTCTGCGCAAGTAATGGATAAGACTGAGGAAATGGTACGTGGTTTATTCAAAGAGTTATTGAATGTTGATTTGGG
CGAGTTCCCTAAGATGACATTCGAAGAAGCGATGCGTCGTTTCGGTTCTGATAAGCCAGATTTACGTAACCCGCTTGAAT
TAATCGATGTTGCAGATATCGTCAAAGAAGTTGAGTTCGCAGTATTTAATGGTCCAGCAAACGATCCAGAAGGCCGTGTT
GCTGTACTAAGTATTCCTGGCGGCGCGAAACTTTCACGTAAGCAGTTAGATGAATACGCAAAGTATGTGACTATTTACGG
CGCTAAAGGCCTAGCTTGGATGAAAGTTAATGACCTTGCTCAAGGCATGGAAGGCATTCAGTCTCCAGTACTTAAGTTCC
TAAGTGAAGATGTGGTTAACGCATTGCTTGAGCGCACAGGTGCTCAAACTGGCGATCTAATCCTATTCGGTGCAGATAAA
GCTAATATCGTTGCAGAAGCGATGGGCGCACTACGTCTTAAAGCCGGTGAAGACTTTGACTTGCTACAAGGCGAGTGGAA
GCCTCTTTGGGTTGTTGACTTCCCAATGTTTGAACGCACATCTGATGGCGGCCTGCATGCAATGCACCACCCATTCACAG
CGCCAAGCAACATGACGCCTGAAGAACTAGAAGCGAATCCAATTGGTGCGATTTCTGATGCTTATGACATGGTACTAAAC
GGTTGTGAATTAGGCGGCGGTTCGGTGCGTATTCATGACAGCAAGATGCAGTCAGCTGTATTTAGAATTCTAGGCATTAA
TGACGAAGAAGCGAGCGAGAAGTTTGGCTTCCTACTCGAAGCATTACGCTACGGCACACCACCACATGCAGGTTTGGCTT
TCGGTTTAGACCGTATCATCATGTTGATGACGGGCGCGAGCTCAATCCGTGACGTGATGGCATTCCCGAAAACGACGACA
GCGGCTTGTCCGTTGACGAATGCACCAGGATTTGCCAATCCAGTGCAGTTAGCTGAACTAGGTGTCAGTGTCGTCGAAGC
GGAGCCAAAAGCTGACGCTAAAGACACAGAATAA

Upstream 100 bases:

>100_bases
AGTTATTTGGAAAACGGCAATATTTCTCTATAATGCCGCTTTTGTCTTGTTTTGAACCAGATCCTCAAGCATCGCTTTGC
GGAATAAAGGAAAGAGTTAA

Downstream 100 bases:

>100_bases
TAAAACCCATAAAGAGCCGAGTTAAGACTCGGCTTTTTTTTCGGTAAAATATACCCAAGAGACTTGATAATTACGAATCT
TTAGCTCTTACAGGTCGTTT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGIVQVVYDPDLPEVFDVASTLRSEFCVQIKGLVRARPD
SQINADMRTGEVEILGLELTILNSSAPLPINMDKNQHNTEEQRLKYRYLDLRRPEMADRIVFRSKVTSAVRRFLDGNGFL
DIETPILTKATPEGARDYLVPSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MTSAQVMDKTEEMVRGLFKELLNVDLGEFPKMTFEEAMRRFGSDKPDLRNPLELIDVADIVKEVEFAVFNGPANDPEGRV
AVLSIPGGAKLSRKQLDEYAKYVTIYGAKGLAWMKVNDLAQGMEGIQSPVLKFLSEDVVNALLERTGAQTGDLILFGADK
ANIVAEAMGALRLKAGEDFDLLQGEWKPLWVVDFPMFERTSDGGLHAMHHPFTAPSNMTPEELEANPIGAISDAYDMVLN
GCELGGGSVRIHDSKMQSAVFRILGINDEEASEKFGFLLEALRYGTPPHAGLAFGLDRIIMLMTGASSIRDVMAFPKTTT
AACPLTNAPGFANPVQLAELGVSVVEAEPKADAKDTE

Sequences:

>Translated_597_residues
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGIVQVVYDPDLPEVFDVASTLRSEFCVQIKGLVRARPD
SQINADMRTGEVEILGLELTILNSSAPLPINMDKNQHNTEEQRLKYRYLDLRRPEMADRIVFRSKVTSAVRRFLDGNGFL
DIETPILTKATPEGARDYLVPSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MTSAQVMDKTEEMVRGLFKELLNVDLGEFPKMTFEEAMRRFGSDKPDLRNPLELIDVADIVKEVEFAVFNGPANDPEGRV
AVLSIPGGAKLSRKQLDEYAKYVTIYGAKGLAWMKVNDLAQGMEGIQSPVLKFLSEDVVNALLERTGAQTGDLILFGADK
ANIVAEAMGALRLKAGEDFDLLQGEWKPLWVVDFPMFERTSDGGLHAMHHPFTAPSNMTPEELEANPIGAISDAYDMVLN
GCELGGGSVRIHDSKMQSAVFRILGINDEEASEKFGFLLEALRYGTPPHAGLAFGLDRIIMLMTGASSIRDVMAFPKTTT
AACPLTNAPGFANPVQLAELGVSVVEAEPKADAKDTE
>Mature_597_residues
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGIVQVVYDPDLPEVFDVASTLRSEFCVQIKGLVRARPD
SQINADMRTGEVEILGLELTILNSSAPLPINMDKNQHNTEEQRLKYRYLDLRRPEMADRIVFRSKVTSAVRRFLDGNGFL
DIETPILTKATPEGARDYLVPSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MTSAQVMDKTEEMVRGLFKELLNVDLGEFPKMTFEEAMRRFGSDKPDLRNPLELIDVADIVKEVEFAVFNGPANDPEGRV
AVLSIPGGAKLSRKQLDEYAKYVTIYGAKGLAWMKVNDLAQGMEGIQSPVLKFLSEDVVNALLERTGAQTGDLILFGADK
ANIVAEAMGALRLKAGEDFDLLQGEWKPLWVVDFPMFERTSDGGLHAMHHPFTAPSNMTPEELEANPIGAISDAYDMVLN
GCELGGGSVRIHDSKMQSAVFRILGINDEEASEKFGFLLEALRYGTPPHAGLAFGLDRIIMLMTGASSIRDVMAFPKTTT
AACPLTNAPGFANPVQLAELGVSVVEAEPKADAKDTE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=604, Percent_Identity=38.7417218543046, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI5031815, Length=256, Percent_Identity=28.125, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI194272210, Length=256, Percent_Identity=28.125, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI45439306, Length=268, Percent_Identity=26.865671641791, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI226491502, Length=255, Percent_Identity=27.4509803921569, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4758762, Length=258, Percent_Identity=25.1937984496124, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788173, Length=587, Percent_Identity=67.8023850085179, Blast_Score=847, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=294, Percent_Identity=28.2312925170068, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1789256, Length=292, Percent_Identity=27.7397260273973, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=609, Percent_Identity=33.8259441707718, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI71984122, Length=307, Percent_Identity=28.6644951140065, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=278, Percent_Identity=26.978417266187, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994340, Length=140, Percent_Identity=32.1428571428571, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=140, Percent_Identity=32.1428571428571, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=140, Percent_Identity=32.1428571428571, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=615, Percent_Identity=33.3333333333333, Blast_Score=279, Evalue=9e-76,
Organism=Saccharomyces cerevisiae, GI6323011, Length=268, Percent_Identity=27.6119402985075, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=143, Percent_Identity=29.3706293706294, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324256, Length=123, Percent_Identity=26.8292682926829, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24584738, Length=615, Percent_Identity=36.4227642276423, Blast_Score=335, Evalue=6e-92,
Organism=Drosophila melanogaster, GI24640849, Length=255, Percent_Identity=28.6274509803922, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640851, Length=255, Percent_Identity=28.6274509803922, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136276, Length=253, Percent_Identity=28.8537549407115, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921528, Length=304, Percent_Identity=25, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66353; Mature: 66353

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGIVQVVYDPDLPEVFDVA
CCCCCCCCCCHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHH
STLRSEFCVQIKGLVRARPDSQINADMRTGEVEILGLELTILNSSAPLPINMDKNQHNTE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEECCCCCCCCH
EQRLKYRYLDLRRPEMADRIVFRSKVTSAVRRFLDGNGFLDIETPILTKATPEGARDYLV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCCC
PSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
CCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHH
MTSAQVMDKTEEMVRGLFKELLNVDLGEFPKMTFEEAMRRFGSDKPDLRNPLELIDVADI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VKEVEFAVFNGPANDPEGRVAVLSIPGGAKLSRKQLDEYAKYVTIYGAKGLAWMKVNDLA
HHHHHHEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEECCCCEEEEEHHHHH
QGMEGIQSPVLKFLSEDVVNALLERTGAQTGDLILFGADKANIVAEAMGALRLKAGEDFD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHEECCCCCCH
LLQGEWKPLWVVDFPMFERTSDGGLHAMHHPFTAPSNMTPEELEANPIGAISDAYDMVLN
HHCCCCCEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHC
GCELGGGSVRIHDSKMQSAVFRILGINDEEASEKFGFLLEALRYGTPPHAGLAFGLDRII
CCCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MLMTGASSIRDVMAFPKTTTAACPLTNAPGFANPVQLAELGVSVVEAEPKADAKDTE
HHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGIVQVVYDPDLPEVFDVA
CCCCCCCCCCHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHH
STLRSEFCVQIKGLVRARPDSQINADMRTGEVEILGLELTILNSSAPLPINMDKNQHNTE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEECCCCCCCCH
EQRLKYRYLDLRRPEMADRIVFRSKVTSAVRRFLDGNGFLDIETPILTKATPEGARDYLV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCCC
PSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
CCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHH
MTSAQVMDKTEEMVRGLFKELLNVDLGEFPKMTFEEAMRRFGSDKPDLRNPLELIDVADI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VKEVEFAVFNGPANDPEGRVAVLSIPGGAKLSRKQLDEYAKYVTIYGAKGLAWMKVNDLA
HHHHHHEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEECCCCEEEEEHHHHH
QGMEGIQSPVLKFLSEDVVNALLERTGAQTGDLILFGADKANIVAEAMGALRLKAGEDFD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHEECCCCCCH
LLQGEWKPLWVVDFPMFERTSDGGLHAMHHPFTAPSNMTPEELEANPIGAISDAYDMVLN
HHCCCCCEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHC
GCELGGGSVRIHDSKMQSAVFRILGINDEEASEKFGFLLEALRYGTPPHAGLAFGLDRII
CCCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MLMTGASSIRDVMAFPKTTTAACPLTNAPGFANPVQLAELGVSVVEAEPKADAKDTE
HHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA