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The map label for this gene is ruvC [H]

Identifier: 157962185

GI number: 157962185

Start: 2882188

End: 2882709

Strand: Reverse

Name: ruvC [H]

Synonym: Spea_2364

Alternate gene names: 157962185

Gene position: 2882709-2882188 (Counterclockwise)

Preceding gene: 157962186

Following gene: 157962184

Centisome position: 55.71

GC content: 48.28

Gene sequence:

>522_bases
ATGGCCATTATTTTGGGGGTCGACCCTGGGTCTAGGATCACTGGATACGGTGTTATTAAATGTGTAGGCAGGCAACAAGT
TTATGTTGGCAGTGGCTGTATTCGTACCAGCTCTGATGAGTTGCCATTACGCCTAAAGCAGATTTTCGATGGTTTATCTG
AAATTATCAGACAATATCAACCCGATGAATTTGCGATTGAGCGGGTGTTTATGGCCAAAAATGCGGATTCAGCATTAAAA
CTTGGTCAAGCAAGAGGCGCAGCTATCGTCGCGGCAACCGCGGCTAATCTGCCCGTTGCCGAATACAGTGCAACTCAGAT
TAAAAGTGCGGTAGTGGGCACTGGGCGCGCGCAAAAAACGCAAGTGCAGCACATGATCCAGCAACTACTAAAACTCCCAA
GCGCGCCACAAGCCGATGCCGCTGATGCGCTTGGTGTGGCTGTGTGTCATTATCACACGAGTCAGAGTCTAGTTGCCTTG
GCTGGCAGAGCAAATGTAAGAACATATGGACGATATAAATGA

Upstream 100 bases:

>100_bases
CGTTTGATTGATAATCTTGAAGATCATGATGATGTTCAAGAGGTGTATCACAACGCAGAAATTTCTGACGAAGTGATGGA
AGGGCTGGAATAAGTAAAAT

Downstream 100 bases:

>100_bases
TAGGTCGTATTCGCGGAATACTTATTGAGAAACAGGCACCAGAAGTTCTGGTCGATGTTTCTGGAGTCGGTTATGAGCTT
CAAATGCCACTCACCAGCTT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MAIILGVDPGSRITGYGVIKCVGRQQVYVGSGCIRTSSDELPLRLKQIFDGLSEIIRQYQPDEFAIERVFMAKNADSALK
LGQARGAAIVAATAANLPVAEYSATQIKSAVVGTGRAQKTQVQHMIQQLLKLPSAPQADAADALGVAVCHYHTSQSLVAL
AGRANVRTYGRYK

Sequences:

>Translated_173_residues
MAIILGVDPGSRITGYGVIKCVGRQQVYVGSGCIRTSSDELPLRLKQIFDGLSEIIRQYQPDEFAIERVFMAKNADSALK
LGQARGAAIVAATAANLPVAEYSATQIKSAVVGTGRAQKTQVQHMIQQLLKLPSAPQADAADALGVAVCHYHTSQSLVAL
AGRANVRTYGRYK
>Mature_172_residues
AIILGVDPGSRITGYGVIKCVGRQQVYVGSGCIRTSSDELPLRLKQIFDGLSEIIRQYQPDEFAIERVFMAKNADSALKL
GQARGAAIVAATAANLPVAEYSATQIKSAVVGTGRAQKTQVQHMIQQLLKLPSAPQADAADALGVAVCHYHTSQSLVALA
GRANVRTYGRYK

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=161, Percent_Identity=67.7018633540373, Blast_Score=208, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 18459; Mature: 18327

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIILGVDPGSRITGYGVIKCVGRQQVYVGSGCIRTSSDELPLRLKQIFDGLSEIIRQYQ
CEEEEECCCCCCCCCCEEEEEECCCEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHCC
PDEFAIERVFMAKNADSALKLGQARGAAIVAATAANLPVAEYSATQIKSAVVGTGRAQKT
CCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHH
QVQHMIQQLLKLPSAPQADAADALGVAVCHYHTSQSLVALAGRANVRTYGRYK
HHHHHHHHHHHCCCCCCCCCHHHHHHHEEEECCCCHHEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AIILGVDPGSRITGYGVIKCVGRQQVYVGSGCIRTSSDELPLRLKQIFDGLSEIIRQYQ
EEEEECCCCCCCCCCEEEEEECCCEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHCC
PDEFAIERVFMAKNADSALKLGQARGAAIVAATAANLPVAEYSATQIKSAVVGTGRAQKT
CCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHH
QVQHMIQQLLKLPSAPQADAADALGVAVCHYHTSQSLVALAGRANVRTYGRYK
HHHHHHHHHHHCCCCCCCCCHHHHHHHEEEECCCCHHEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA