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The map label for this gene is emrB [H]

Identifier: 157962011

GI number: 157962011

Start: 2654786

End: 2656330

Strand: Reverse

Name: emrB [H]

Synonym: Spea_2190

Alternate gene names: 157962011

Gene position: 2656330-2654786 (Counterclockwise)

Preceding gene: 157962012

Following gene: 157962009

Centisome position: 51.33

GC content: 47.44

Gene sequence:

>1545_bases
ATGACCGAGCTCTCTCAAGCATCGTCAAGCCCTGGTGATGCTCAAGTATTAAGTGAAGGCAGTCGTAAACTGGTGACCTT
GGCGGTGATGTTATCCGCTATCATGGTCTTACTCGACATGACGATTGCGAATGTTGCCCTACCGCATATGATGGGCGCAT
TGGGGGTGACGTCCGATCAAGTCACTTGGGTACTGACCTCCTACTCTATGGCTGAGGCGATATTTATCCCGCTAGCCAGT
TTTCTTGCGCTTAAATTTGGTATTCGTAATCTACTATTGATCTCAGTCAGTGGATTTATTGTCAGCTCGGCTCTTTGTGG
CCAAGCCGATACCATTACTGAAATGGTGACCTTTAGGGTGATGCAAGGAGCATTTGGCGCTTCGGTGATCCCTCTATCTC
AGTCAATCATGGTGCAGATCTATCCCGCTAATCAGCGCGGTAAGGCGATGGCACTATTTAGTGTTGGCGTATTGCTTGGG
CCAATTCTAGGGCCGACACTAGGTGGGATCATTACCGAAAACATGGATTGGCGCTGGATTTTTTACGTTAATCTACCCGT
TGGCGCCATCTGCCTTACGCTGATTTACACCTTTGTTAAGCTCAGTGGTAAAGGTAAACCTAAAATTGACTGGCCAATCG
TTATCGCAATGACAATCGGTATTGGTCTGCTGCAGATGGTGCTCGACCGGGGTAATCAAGAATCTTGGTTCGAGTCCAAT
ACCATCTTGTTTTCAACCATAATCAGCGCCATTGCGATTATTTTCTTTGTGGCACGCTCTTTTACGACTAAGAGTGAGAT
CGCTCCTGTTTGGTTATTGCGAGACCGTAACCTTGCAATGTCATGTCTGGTTATGGCTGGCTTCTCGATGGGAATGTTCG
GTATCACTCAGTTGCAACCTATGATGCTAGAGCAGTTATTAAATTACCCGGTAGAAACCACAGGCTTTGCCATGGCGCCA
AGAGGTCTCGCATCAGCATTTGTGCTACTGATGATGGCGCGCTATATGGATAGAATTGATGCCAGGTTGTTGATTGTTAT
TGGCCTGAGTCTTAATGCGCTCGGAACCTATTTGATGACGCAATACTCCCTTGAGATAAACATCTATTGGATTTTATTAC
CCAGTATCATTCAAGGCGCAGGCATGGGCTTAGTGTTTGCGCCTCTTAGCCAGTTGGCCTACGCCACCCTTGCCCCCAAA
GACACAATCGGTGGTGCCGTGGTGTTTAACTTATGCCGCACCATTGGCGGCTCTTTCGGGATCTCTATTGTGAATACTTA
TTTCTTCCGTACCCAGCAGCGGGAATGGAACGAATTAGGCGGTGCACTTTCGCCAACCAATCCAGTTTTACAGCAAGCGG
CACAAGCCCAAGGAACCACTATCACCGACCCGACATTTTTGGCTCAGATGCAAAATATCTTACATCAACAATCAACCTTG
ACGGCGTTCGTATTTACATTCGGTTTTCTGTTGGTCTCCTACCTGTGTCTCATCCCGCTATTGGCCCTGTTTAAACCCAA
ACCTAAAGTTGTGCCCGAGTCGTAA

Upstream 100 bases:

>100_bases
GCTAACGAAAGTGAACACCCTGACACTCCGTTACGGGTGGGCGCTAGCGCGAATGTCACCATAGATACAGTTAGCACAGC
AAACAAACAAGAGCAGTAAC

Downstream 100 bases:

>100_bases
TCATCAAGATCGAAATCATCAAAATAATAGGTAAACTTTTGGTGATTTCATAAGGCGCGGGGAATAACTTCCCGAGTAAA
CTCGAGCCAACAATAGGCTT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTELSQASSSPGDAQVLSEGSRKLVTLAVMLSAIMVLLDMTIANVALPHMMGALGVTSDQVTWVLTSYSMAEAIFIPLAS
FLALKFGIRNLLLISVSGFIVSSALCGQADTITEMVTFRVMQGAFGASVIPLSQSIMVQIYPANQRGKAMALFSVGVLLG
PILGPTLGGIITENMDWRWIFYVNLPVGAICLTLIYTFVKLSGKGKPKIDWPIVIAMTIGIGLLQMVLDRGNQESWFESN
TILFSTIISAIAIIFFVARSFTTKSEIAPVWLLRDRNLAMSCLVMAGFSMGMFGITQLQPMMLEQLLNYPVETTGFAMAP
RGLASAFVLLMMARYMDRIDARLLIVIGLSLNALGTYLMTQYSLEINIYWILLPSIIQGAGMGLVFAPLSQLAYATLAPK
DTIGGAVVFNLCRTIGGSFGISIVNTYFFRTQQREWNELGGALSPTNPVLQQAAQAQGTTITDPTFLAQMQNILHQQSTL
TAFVFTFGFLLVSYLCLIPLLALFKPKPKVVPES

Sequences:

>Translated_514_residues
MTELSQASSSPGDAQVLSEGSRKLVTLAVMLSAIMVLLDMTIANVALPHMMGALGVTSDQVTWVLTSYSMAEAIFIPLAS
FLALKFGIRNLLLISVSGFIVSSALCGQADTITEMVTFRVMQGAFGASVIPLSQSIMVQIYPANQRGKAMALFSVGVLLG
PILGPTLGGIITENMDWRWIFYVNLPVGAICLTLIYTFVKLSGKGKPKIDWPIVIAMTIGIGLLQMVLDRGNQESWFESN
TILFSTIISAIAIIFFVARSFTTKSEIAPVWLLRDRNLAMSCLVMAGFSMGMFGITQLQPMMLEQLLNYPVETTGFAMAP
RGLASAFVLLMMARYMDRIDARLLIVIGLSLNALGTYLMTQYSLEINIYWILLPSIIQGAGMGLVFAPLSQLAYATLAPK
DTIGGAVVFNLCRTIGGSFGISIVNTYFFRTQQREWNELGGALSPTNPVLQQAAQAQGTTITDPTFLAQMQNILHQQSTL
TAFVFTFGFLLVSYLCLIPLLALFKPKPKVVPES
>Mature_513_residues
TELSQASSSPGDAQVLSEGSRKLVTLAVMLSAIMVLLDMTIANVALPHMMGALGVTSDQVTWVLTSYSMAEAIFIPLASF
LALKFGIRNLLLISVSGFIVSSALCGQADTITEMVTFRVMQGAFGASVIPLSQSIMVQIYPANQRGKAMALFSVGVLLGP
ILGPTLGGIITENMDWRWIFYVNLPVGAICLTLIYTFVKLSGKGKPKIDWPIVIAMTIGIGLLQMVLDRGNQESWFESNT
ILFSTIISAIAIIFFVARSFTTKSEIAPVWLLRDRNLAMSCLVMAGFSMGMFGITQLQPMMLEQLLNYPVETTGFAMAPR
GLASAFVLLMMARYMDRIDARLLIVIGLSLNALGTYLMTQYSLEINIYWILLPSIIQGAGMGLVFAPLSQLAYATLAPKD
TIGGAVVFNLCRTIGGSFGISIVNTYFFRTQQREWNELGGALSPTNPVLQQAAQAQGTTITDPTFLAQMQNILHQQSTLT
AFVFTFGFLLVSYLCLIPLLALFKPKPKVVPES

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=499, Percent_Identity=32.2645290581162, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1789042, Length=451, Percent_Identity=33.2594235033259, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87081983, Length=406, Percent_Identity=30.0492610837438, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1790195, Length=401, Percent_Identity=25.6857855361596, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788392, Length=323, Percent_Identity=27.5541795665635, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6325455, Length=153, Percent_Identity=29.4117647058824, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=28.2894736842105, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=181, Percent_Identity=24.8618784530387, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323000, Length=123, Percent_Identity=30.0813008130081, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55837; Mature: 55706

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELSQASSSPGDAQVLSEGSRKLVTLAVMLSAIMVLLDMTIANVALPHMMGALGVTSDQ
CCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VTWVLTSYSMAEAIFIPLASFLALKFGIRNLLLISVSGFIVSSALCGQADTITEMVTFRV
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MQGAFGASVIPLSQSIMVQIYPANQRGKAMALFSVGVLLGPILGPTLGGIITENMDWRWI
HHHCCCCEEEECCCEEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
FYVNLPVGAICLTLIYTFVKLSGKGKPKIDWPIVIAMTIGIGLLQMVLDRGNQESWFESN
EEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
TILFSTIISAIAIIFFVARSFTTKSEIAPVWLLRDRNLAMSCLVMAGFSMGMFGITQLQP
HHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEEECCCHHHHHHHHHHHHHCHHHHHHCCH
MMLEQLLNYPVETTGFAMAPRGLASAFVLLMMARYMDRIDARLLIVIGLSLNALGTYLMT
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHH
QYSLEINIYWILLPSIIQGAGMGLVFAPLSQLAYATLAPKDTIGGAVVFNLCRTIGGSFG
EEEEEEEEEEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
ISIVNTYFFRTQQREWNELGGALSPTNPVLQQAAQAQGTTITDPTFLAQMQNILHQQSTL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
TAFVFTFGFLLVSYLCLIPLLALFKPKPKVVPES
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TELSQASSSPGDAQVLSEGSRKLVTLAVMLSAIMVLLDMTIANVALPHMMGALGVTSDQ
CCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VTWVLTSYSMAEAIFIPLASFLALKFGIRNLLLISVSGFIVSSALCGQADTITEMVTFRV
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MQGAFGASVIPLSQSIMVQIYPANQRGKAMALFSVGVLLGPILGPTLGGIITENMDWRWI
HHHCCCCEEEECCCEEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
FYVNLPVGAICLTLIYTFVKLSGKGKPKIDWPIVIAMTIGIGLLQMVLDRGNQESWFESN
EEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
TILFSTIISAIAIIFFVARSFTTKSEIAPVWLLRDRNLAMSCLVMAGFSMGMFGITQLQP
HHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEEECCCHHHHHHHHHHHHHCHHHHHHCCH
MMLEQLLNYPVETTGFAMAPRGLASAFVLLMMARYMDRIDARLLIVIGLSLNALGTYLMT
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHH
QYSLEINIYWILLPSIIQGAGMGLVFAPLSQLAYATLAPKDTIGGAVVFNLCRTIGGSFG
EEEEEEEEEEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
ISIVNTYFFRTQQREWNELGGALSPTNPVLQQAAQAQGTTITDPTFLAQMQNILHQQSTL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
TAFVFTFGFLLVSYLCLIPLLALFKPKPKVVPES
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]