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The map label for this gene is nylA [H]

Identifier: 157961984

GI number: 157961984

Start: 2617412

End: 2619007

Strand: Direct

Name: nylA [H]

Synonym: Spea_2163

Alternate gene names: 157961984

Gene position: 2617412-2619007 (Clockwise)

Preceding gene: 157961983

Following gene: 157961985

Centisome position: 50.58

GC content: 47.93

Gene sequence:

>1596_bases
ATGGCAATGAACGATAATATTGATAATAAAAGACGTAGTTTGCTTAAAACAGGTACAGCACTTGGTGCTGCAGCATTGAG
TGGAGTTTCATTAATCGCGCAAGGGGCTGACGCCGGTAACAGACAAAAGGCTAAGGTTGCGAGTGCCGATTTACCATTAC
CCGAGTACGATAACCTCGATGTTGTCGCGATGGCTGAATCTCTTAAAAAAGGTGAACTTACTCCTATAGAGTTGTTTGAT
GCGGCTTATGCACGCTTTGAAAGTCGCTCGGCAATCAATGTTGTTGCGGTGGAACATTTTGAACAAGCACGTGAGCATGC
TTTGACGCTAAGTAAAGCGGGCAATAAAGAGCGCGCCGACAGTAACGCGCCACTTCGTGGTGTTCCTTTCGCGTTAAAAG
ATCTGCATATAAAAGTAAAAGGGACTGTTACGACCAATGGTAGCCCACTTTTTAAAGACTCGGTAGCAACCAACAACTCC
ACTCTTACTCAGCGGTATATGGACGCAGGCTTGAATATCATGGCTAAGTTGACCAGCCCTGAGTTTGGCCAAACGGCGAC
CACCGAATCGGCTCTTCACGGTGATACGCTAAATCCTTGGGACCTTGCTCGAACTAGCGGCGGCTCAAGCGGAGGCTCTG
CGGCAACAGTTGCGGCAAGAATATTGCCAGCGGCACATGCAAGTGATGGCGGCGGCTCAATCCGAATTCCTGCATCAAAC
TGTGGTGTATTTGGTTTAAAGCCGAGTAGGGGACGAGTACCAATGGGCCCAGGAGCTATAGAGGGCTGGATGGGATTGTC
AGTTCATAATACGGTGACCCGTTCAGTACGAGATTCGGCACTGTTACTCGACTTAACTCAAGGTAAAGAGGCAGGCTCTC
GCATTACTCACCAAGATCAGAGCTTAATGGCTGCAATTAAAAAAGCGCCAAAAGGCCTGAAAATAGCGGTGATGAATAAC
CATCCCTTCGGCTTGCCCATTCATCCTGACTGCATTAAGGCGGTAAATAAAACTGCCAAACTGTTAGCTGCACTAGGGCA
TCACGTTGAAGAGGCACAGCCCATATTACCTATAGAAGGTATGTTTAAAGGCATGGGGGTCGCTACAACGAGTGGCTTAC
TTTACACCATTCAAGAGCAAGAAAAATTGCTCGGCCGTAAAGCACGAGAAGAGGAGTTTTCACCCATCGTGTGGCGTCAC
CTGCAAAATGCACCTAATTACACCGCTCAGAATGTGTTTAGTGCCCGTAGTGCGTTTGATGATGGCGCAAAGGCATTTGA
CCACTTCTTCAGTGATTACCATTTGATCCTGTCACCGGTAACCGCGGTGCCACCACCTAAAATCGGTGCGCTAAGCTTAA
ATCAACCCTATGATGATTTTGTGCAAGATATTATGTATGCCTCACCGATTACCGCACTGTTCAATATGACGGGCTTACCC
GCTATGTCGGTTCCTTTACACTGGAACGATGATGGCTTACCTATTGGAGTGCAGTTTGCCGGACCTTACGGCGGCGAGCC
CTTGCTGTTATCTTTAGCAGCGCAGTTAGAGCAAGCATCGCCGTGGGCAGATAAATTACCAGGGTTTATTAGTTAA

Upstream 100 bases:

>100_bases
CCTGCAGCACAAAAAGTATGGGGTAGCTAAGGATTTTAGCTAAAAGCCTTTAGCTGAATTGAGACAATTGACCACGATAA
TGGATTAGCACCGAGGACAA

Downstream 100 bases:

>100_bases
GCTTCGAATGAAGCTTGAATACATGTTTTATCTACGTATTTCGGCAACTCCGATGGGGAACGGTGTTTTTCTGTATGTGC
CATAACTTACTTTTGCCCCA

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MAMNDNIDNKRRSLLKTGTALGAAALSGVSLIAQGADAGNRQKAKVASADLPLPEYDNLDVVAMAESLKKGELTPIELFD
AAYARFESRSAINVVAVEHFEQAREHALTLSKAGNKERADSNAPLRGVPFALKDLHIKVKGTVTTNGSPLFKDSVATNNS
TLTQRYMDAGLNIMAKLTSPEFGQTATTESALHGDTLNPWDLARTSGGSSGGSAATVAARILPAAHASDGGGSIRIPASN
CGVFGLKPSRGRVPMGPGAIEGWMGLSVHNTVTRSVRDSALLLDLTQGKEAGSRITHQDQSLMAAIKKAPKGLKIAVMNN
HPFGLPIHPDCIKAVNKTAKLLAALGHHVEEAQPILPIEGMFKGMGVATTSGLLYTIQEQEKLLGRKAREEEFSPIVWRH
LQNAPNYTAQNVFSARSAFDDGAKAFDHFFSDYHLILSPVTAVPPPKIGALSLNQPYDDFVQDIMYASPITALFNMTGLP
AMSVPLHWNDDGLPIGVQFAGPYGGEPLLLSLAAQLEQASPWADKLPGFIS

Sequences:

>Translated_531_residues
MAMNDNIDNKRRSLLKTGTALGAAALSGVSLIAQGADAGNRQKAKVASADLPLPEYDNLDVVAMAESLKKGELTPIELFD
AAYARFESRSAINVVAVEHFEQAREHALTLSKAGNKERADSNAPLRGVPFALKDLHIKVKGTVTTNGSPLFKDSVATNNS
TLTQRYMDAGLNIMAKLTSPEFGQTATTESALHGDTLNPWDLARTSGGSSGGSAATVAARILPAAHASDGGGSIRIPASN
CGVFGLKPSRGRVPMGPGAIEGWMGLSVHNTVTRSVRDSALLLDLTQGKEAGSRITHQDQSLMAAIKKAPKGLKIAVMNN
HPFGLPIHPDCIKAVNKTAKLLAALGHHVEEAQPILPIEGMFKGMGVATTSGLLYTIQEQEKLLGRKAREEEFSPIVWRH
LQNAPNYTAQNVFSARSAFDDGAKAFDHFFSDYHLILSPVTAVPPPKIGALSLNQPYDDFVQDIMYASPITALFNMTGLP
AMSVPLHWNDDGLPIGVQFAGPYGGEPLLLSLAAQLEQASPWADKLPGFIS
>Mature_530_residues
AMNDNIDNKRRSLLKTGTALGAAALSGVSLIAQGADAGNRQKAKVASADLPLPEYDNLDVVAMAESLKKGELTPIELFDA
AYARFESRSAINVVAVEHFEQAREHALTLSKAGNKERADSNAPLRGVPFALKDLHIKVKGTVTTNGSPLFKDSVATNNST
LTQRYMDAGLNIMAKLTSPEFGQTATTESALHGDTLNPWDLARTSGGSSGGSAATVAARILPAAHASDGGGSIRIPASNC
GVFGLKPSRGRVPMGPGAIEGWMGLSVHNTVTRSVRDSALLLDLTQGKEAGSRITHQDQSLMAAIKKAPKGLKIAVMNNH
PFGLPIHPDCIKAVNKTAKLLAALGHHVEEAQPILPIEGMFKGMGVATTSGLLYTIQEQEKLLGRKAREEEFSPIVWRHL
QNAPNYTAQNVFSARSAFDDGAKAFDHFFSDYHLILSPVTAVPPPKIGALSLNQPYDDFVQDIMYASPITALFNMTGLPA
MSVPLHWNDDGLPIGVQFAGPYGGEPLLLSLAAQLEQASPWADKLPGFIS

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=517, Percent_Identity=26.8858800773694, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI195972892, Length=505, Percent_Identity=23.7623762376238, Blast_Score=92, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=305, Percent_Identity=27.2131147540984, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=552, Percent_Identity=24.6376811594203, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=317, Percent_Identity=27.4447949526814, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=451, Percent_Identity=25.2771618625277, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=303, Percent_Identity=28.7128712871287, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=299, Percent_Identity=28.7625418060201, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=521, Percent_Identity=25.3358925143954, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=197, Percent_Identity=35.0253807106599, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=406, Percent_Identity=25.8620689655172, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644968, Length=517, Percent_Identity=26.3056092843327, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078093, Length=491, Percent_Identity=27.4949083503055, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21356731, Length=199, Percent_Identity=35.1758793969849, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI45550774, Length=524, Percent_Identity=24.236641221374, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648435, Length=524, Percent_Identity=24.236641221374, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648437, Length=524, Percent_Identity=24.236641221374, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648113, Length=433, Percent_Identity=29.0993071593533, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648441, Length=444, Percent_Identity=24.7747747747748, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648439, Length=444, Percent_Identity=24.7747747747748, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652985, Length=194, Percent_Identity=31.9587628865979, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19922090, Length=194, Percent_Identity=31.9587628865979, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24652981, Length=194, Percent_Identity=31.9587628865979, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24652983, Length=194, Percent_Identity=31.9587628865979, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 56450; Mature: 56319

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMNDNIDNKRRSLLKTGTALGAAALSGVSLIAQGADAGNRQKAKVASADLPLPEYDNLD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEECCCCCCCCCCCCCC
VVAMAESLKKGELTPIELFDAAYARFESRSAINVVAVEHFEQAREHALTLSKAGNKERAD
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCC
SNAPLRGVPFALKDLHIKVKGTVTTNGSPLFKDSVATNNSTLTQRYMDAGLNIMAKLTSP
CCCCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCC
EFGQTATTESALHGDTLNPWDLARTSGGSSGGSAATVAARILPAAHASDGGGSIRIPASN
CCCCCCCCCHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
CGVFGLKPSRGRVPMGPGAIEGWMGLSVHNTVTRSVRDSALLLDLTQGKEAGSRITHQDQ
CCEEECCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHH
SLMAAIKKAPKGLKIAVMNNHPFGLPIHPDCIKAVNKTAKLLAALGHHVEEAQPILPIEG
HHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
MFKGMGVATTSGLLYTIQEQEKLLGRKAREEEFSPIVWRHLQNAPNYTAQNVFSARSAFD
HHCCCCEEECCCEEEEEEHHHHHHCCHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCC
DGAKAFDHFFSDYHLILSPVTAVPPPKIGALSLNQPYDDFVQDIMYASPITALFNMTGLP
HHHHHHHHHHCCCEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
AMSVPLHWNDDGLPIGVQFAGPYGGEPLLLSLAAQLEQASPWADKLPGFIS
EEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCC
>Mature Secondary Structure 
AMNDNIDNKRRSLLKTGTALGAAALSGVSLIAQGADAGNRQKAKVASADLPLPEYDNLD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEECCCCCCCCCCCCCC
VVAMAESLKKGELTPIELFDAAYARFESRSAINVVAVEHFEQAREHALTLSKAGNKERAD
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCC
SNAPLRGVPFALKDLHIKVKGTVTTNGSPLFKDSVATNNSTLTQRYMDAGLNIMAKLTSP
CCCCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCC
EFGQTATTESALHGDTLNPWDLARTSGGSSGGSAATVAARILPAAHASDGGGSIRIPASN
CCCCCCCCCHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
CGVFGLKPSRGRVPMGPGAIEGWMGLSVHNTVTRSVRDSALLLDLTQGKEAGSRITHQDQ
CCEEECCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHH
SLMAAIKKAPKGLKIAVMNNHPFGLPIHPDCIKAVNKTAKLLAALGHHVEEAQPILPIEG
HHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
MFKGMGVATTSGLLYTIQEQEKLLGRKAREEEFSPIVWRHLQNAPNYTAQNVFSARSAFD
HHCCCCEEECCCEEEEEEHHHHHHCCHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCC
DGAKAFDHFFSDYHLILSPVTAVPPPKIGALSLNQPYDDFVQDIMYASPITALFNMTGLP
HHHHHHHHHHCCCEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
AMSVPLHWNDDGLPIGVQFAGPYGGEPLLLSLAAQLEQASPWADKLPGFIS
EEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]