BacMap

Definition	Shewanella pealeana ATCC 700345 chromosome, complete genome.
Accession	NC_009901
Length	5,174,581

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The map label for this gene is echA8 [H]

Identifier: 157961957

GI number: 157961957

Start: 2588001

End: 2588783

Strand: Reverse

Name: echA8 [H]

Synonym: Spea_2136

Alternate gene names: 157961957

Gene position: 2588783-2588001 (Counterclockwise)

Preceding gene: 157961967

Following gene: 157961949

Centisome position: 50.03

GC content: 47.51

Gene sequence:

>783_bases
TTGGAGAATCAACAACAGACGGTGTTATTAGAAAGGCCGGAGGAAGGCGTAGCATTATTAACTCTGAATAGACCCGCGGC
GACCAATGCGTTAAGTCTTGAGTTACAACAGTGTTTATCTGATCACTTTAGCGCTTTATCTAATGATCCCACAGTACGAT
GTATAGTGCTGACCGGAGGCGATAAGGTTTTTGCAGCTGGCGGTGATATAACTAGCCTTATCGAAGCCAGTCCAATTGAT
ATTTATCAGCGCCATACTGAAAGACTATGGGCGCCTATAGAGCAATGCCCAAAACCCGTTATAGCTGCTGTGTGTGGTTA
TGCTTATGGAGGGGGCTGCGAGCTTGCGATGTTAGCCGATATTATAATAGCTGGCGAAACTGCGGCGTTTTGCCAGCCAG
AAATCGCCATAGGGATCATGCCCGGTATCGGTGGCACTCAAAGATTGGTACGTGCAGTAGGTAAGGCAAAAGCCATGCAA
ATGGCACTAACAGGACGCCCTATTACAGCGCAAGAAGCATTTACTGCCGGATTAGTGAGTGAAGTGTGCGACGATGATAG
CGTACTAGAACAAGCCTTAACAAATGCTAGACGTATCGCTCGTATGCCTCCTTTAGCGGCTGAGCAAATAAAGGAAGTTA
TACTTGCAGGCATGGACGCATCACTCCCTGCTGCCATGGCGCTAGAGCGTAAAGCCAATGCGTTATTGTTTGCTTCTGAA
GATCAGCGTGAAGGCATGGTCGCTTTTCTCGAAAAAAGACGAGCGGTGTTTACCGGCAAATAA

Upstream 100 bases:

>100_bases
AGTGGCTATGAATATGAATAAAATCGAATTTGTTGCTTTTTCTGTACTATCACGTCAACCAACAGACATCGATGCACGGC
ACTACTTGAGGAGAGATAAG

Downstream 100 bases:

>100_bases
TATCATCACGGCGCTTCGCCAAATGCGCCGTTTACATCTAACGTAGAGCAATGAGCTAGATAACAGGTTACTCCTCCGTT
CTAATGTCATTACTTTTTGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MENQQQTVLLERPEEGVALLTLNRPAATNALSLELQQCLSDHFSALSNDPTVRCIVLTGGDKVFAAGGDITSLIEASPID
IYQRHTERLWAPIEQCPKPVIAAVCGYAYGGGCELAMLADIIIAGETAAFCQPEIAIGIMPGIGGTQRLVRAVGKAKAMQ
MALTGRPITAQEAFTAGLVSEVCDDDSVLEQALTNARRIARMPPLAAEQIKEVILAGMDASLPAAMALERKANALLFASE
DQREGMVAFLEKRRAVFTGK

Sequences:

>Translated_260_residues
MENQQQTVLLERPEEGVALLTLNRPAATNALSLELQQCLSDHFSALSNDPTVRCIVLTGGDKVFAAGGDITSLIEASPID
IYQRHTERLWAPIEQCPKPVIAAVCGYAYGGGCELAMLADIIIAGETAAFCQPEIAIGIMPGIGGTQRLVRAVGKAKAMQ
MALTGRPITAQEAFTAGLVSEVCDDDSVLEQALTNARRIARMPPLAAEQIKEVILAGMDASLPAAMALERKANALLFASE
DQREGMVAFLEKRRAVFTGK
>Mature_260_residues
MENQQQTVLLERPEEGVALLTLNRPAATNALSLELQQCLSDHFSALSNDPTVRCIVLTGGDKVFAAGGDITSLIEASPID
IYQRHTERLWAPIEQCPKPVIAAVCGYAYGGGCELAMLADIIIAGETAAFCQPEIAIGIMPGIGGTQRLVRAVGKAKAMQ
MALTGRPITAQEAFTAGLVSEVCDDDSVLEQALTNARRIARMPPLAAEQIKEVILAGMDASLPAAMALERKANALLFASE
DQREGMVAFLEKRRAVFTGK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=241, Percent_Identity=49.792531120332, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=30.9803921568627, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI70995211, Length=268, Percent_Identity=29.4776119402985, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI4502327, Length=266, Percent_Identity=32.7067669172932, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI45643119, Length=246, Percent_Identity=28.8617886178862, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI260275230, Length=246, Percent_Identity=28.8617886178862, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI260274832, Length=246, Percent_Identity=28.8617886178862, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI68989263, Length=231, Percent_Identity=31.1688311688312, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI37594469, Length=196, Percent_Identity=32.1428571428571, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI37594471, Length=196, Percent_Identity=32.1428571428571, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI150378541, Length=260, Percent_Identity=30, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI20127408, Length=175, Percent_Identity=33.7142857142857, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI213417737, Length=233, Percent_Identity=24.8927038626609, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI157694516, Length=233, Percent_Identity=24.8927038626609, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI221136756, Length=250, Percent_Identity=26, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI221136753, Length=250, Percent_Identity=26, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI221307494, Length=250, Percent_Identity=26, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=23.4375, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=23.4375, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4757968, Length=258, Percent_Identity=23.6434108527132, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI49355787, Length=258, Percent_Identity=23.6434108527132, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI62530384, Length=262, Percent_Identity=27.0992366412214, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4757966, Length=232, Percent_Identity=24.1379310344828, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI51538581, Length=232, Percent_Identity=24.1379310344828, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI116235487, Length=250, Percent_Identity=24.8, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787659, Length=247, Percent_Identity=46.5587044534413, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=36.2934362934363, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI221142681, Length=252, Percent_Identity=34.5238095238095, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=33.8582677165354, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=30.1526717557252, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790281, Length=228, Percent_Identity=28.5087719298246, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788682, Length=210, Percent_Identity=30.952380952381, Blast_Score=84, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=42.914979757085, Blast_Score=197, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=43.8524590163934, Blast_Score=196, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17536985, Length=275, Percent_Identity=27.6363636363636, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=243, Percent_Identity=31.6872427983539, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=241, Percent_Identity=29.8755186721992, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=265, Percent_Identity=25.2830188679245, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=209, Percent_Identity=32.0574162679426, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=194, Percent_Identity=30.9278350515464, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=194, Percent_Identity=30.9278350515464, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=261, Percent_Identity=30.6513409961686, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=173, Percent_Identity=32.3699421965318, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=173, Percent_Identity=31.2138728323699, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=173, Percent_Identity=31.2138728323699, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=211, Percent_Identity=26.0663507109005, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=173, Percent_Identity=31.2138728323699, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=214, Percent_Identity=26.1682242990654, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20129971, Length=259, Percent_Identity=44.4015444015444, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24653477, Length=259, Percent_Identity=44.4015444015444, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=33.4600760456274, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19920382, Length=236, Percent_Identity=34.7457627118644, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=27.4131274131274, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28571729, Length=197, Percent_Identity=30.9644670050761, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28571730, Length=197, Percent_Identity=30.9644670050761, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21357171, Length=252, Percent_Identity=28.1746031746032, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24650670, Length=218, Percent_Identity=28.8990825688073, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24654903, Length=237, Percent_Identity=23.2067510548523, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27708; Mature: 27708

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENQQQTVLLERPEEGVALLTLNRPAATNALSLELQQCLSDHFSALSNDPTVRCIVLTGG
CCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
DKVFAAGGDITSLIEASPIDIYQRHTERLWAPIEQCPKPVIAAVCGYAYGGGCELAMLAD
CEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHH
IIIAGETAAFCQPEIAIGIMPGIGGTQRLVRAVGKAKAMQMALTGRPITAQEAFTAGLVS
HHHCCCCHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EVCDDDSVLEQALTNARRIARMPPLAAEQIKEVILAGMDASLPAAMALERKANALLFASE
HHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECC
DQREGMVAFLEKRRAVFTGK
CCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MENQQQTVLLERPEEGVALLTLNRPAATNALSLELQQCLSDHFSALSNDPTVRCIVLTGG
CCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
DKVFAAGGDITSLIEASPIDIYQRHTERLWAPIEQCPKPVIAAVCGYAYGGGCELAMLAD
CEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHH
IIIAGETAAFCQPEIAIGIMPGIGGTQRLVRAVGKAKAMQMALTGRPITAQEAFTAGLVS
HHHCCCCHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EVCDDDSVLEQALTNARRIARMPPLAAEQIKEVILAGMDASLPAAMALERKANALLFASE
HHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECC
DQREGMVAFLEKRRAVFTGK
CCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]