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The map label for this gene is mhpE [C]

Identifier: 157961937

GI number: 157961937

Start: 2560582

End: 2561604

Strand: Reverse

Name: mhpE [C]

Synonym: Spea_2116

Alternate gene names: 157961937

Gene position: 2561604-2560582 (Counterclockwise)

Preceding gene: 157961938

Following gene: 157961936

Centisome position: 49.5

GC content: 47.21

Gene sequence:

>1023_bases
ATGAACTTAACTGGAAAAAAAGTTATCTTACACGATATGTCATTAAGAGACGGCATGCATGCTCGCCAACACCAAATTAG
TTTGAAAGAGATGGTTGATGTTGCGACAGGACTCGATGCTGCAGGCGTACCTTTAATCGAAGTGACCCACGGTGATGGCC
TTGGCGGTGCCTCACTCAATTACGGATTTCCTGCGCACACCGATGAAGAATACTTAAGCGCGGTGATCCCTAAAATGAAG
CAGGCAAAAGTATCTGCTTTGTTGTTACCTGGGATAGGTACGGGTGATCATTTGCGCATGGCCCATGACCTCGGGGTTAA
TACTATCCGTGTTGCAACCCATTGTACTGAGGCGGATGTATCTGGGCAGCATATTTCGTTATCACGTGAATTAGGCCTAG
ATACAGTAGGGTTTCTTATGATGGCTCATATGGTTAGCCCTGATAAATTACTTGAGCAAGCCAAACTGATGGAGTCCTAC
GGTGCGAACTGTATTTACTGCACCGACTCTGCAGGTTATATGCTGCCAGGAGACGTAACTTCGCATATTGAGCGCTTACG
CGGAGAGCTAAAAAGCGATACCCAAATTGGTTTTCATGGCCACCATAATATGGGAATGAGTATCGCGAACTCCCTAGCTG
CAATCCAAGCTGGTGCTGAGCGAATAGATGGCTCAGTCGCAGGTTTAGGAGCTGGAGCAGGCAACACCCCCCTAGAGGTA
TTCGTTGCCGTTCTTGAGCGTATGCAAGTAAGCCACGGGATTAATTTGTATGACATTATGGACGTAGCTGAAGATCTGGT
TACCCCGATGATGGATCAGCCTATAAGAATCGACAGAGATGCACTGACGTTAGGCTATGCAGGCGTTTACTCATCATTCT
TATTATTTGCTCAGAGAGCCGAGAAAAAACATGGCATTCCAGCGAGAGATATACTGCTCGAACTGGGTAGGCGTGGCACT
GTTGGCGGCCAAGAAGATATGATTGACGACACGGCAATGAATATGGCCAGAGAACGCGCATAA

Upstream 100 bases:

>100_bases
TGCAGCAGCCAGAACAGCAGAAATGCTAGCGTCCAAAATGCTCAATGTTAAAACCCATCTTAACGTTACCAAAGAAGCGG
CGCTAGCCTAGGAGATCACG

Downstream 100 bases:

>100_bases
CTATGGTCAGCTGCGCTAACAATAACGGACAACAGTGGCGCCGACACGGTCTGCTACTGTTGTTGACCTTATTTTATGGT
GAAACTTTCATCGGTAGGCA

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNLTGKKVILHDMSLRDGMHARQHQISLKEMVDVATGLDAAGVPLIEVTHGDGLGGASLNYGFPAHTDEEYLSAVIPKMK
QAKVSALLLPGIGTGDHLRMAHDLGVNTIRVATHCTEADVSGQHISLSRELGLDTVGFLMMAHMVSPDKLLEQAKLMESY
GANCIYCTDSAGYMLPGDVTSHIERLRGELKSDTQIGFHGHHNMGMSIANSLAAIQAGAERIDGSVAGLGAGAGNTPLEV
FVAVLERMQVSHGINLYDIMDVAEDLVTPMMDQPIRIDRDALTLGYAGVYSSFLLFAQRAEKKHGIPARDILLELGRRGT
VGGQEDMIDDTAMNMARERA

Sequences:

>Translated_340_residues
MNLTGKKVILHDMSLRDGMHARQHQISLKEMVDVATGLDAAGVPLIEVTHGDGLGGASLNYGFPAHTDEEYLSAVIPKMK
QAKVSALLLPGIGTGDHLRMAHDLGVNTIRVATHCTEADVSGQHISLSRELGLDTVGFLMMAHMVSPDKLLEQAKLMESY
GANCIYCTDSAGYMLPGDVTSHIERLRGELKSDTQIGFHGHHNMGMSIANSLAAIQAGAERIDGSVAGLGAGAGNTPLEV
FVAVLERMQVSHGINLYDIMDVAEDLVTPMMDQPIRIDRDALTLGYAGVYSSFLLFAQRAEKKHGIPARDILLELGRRGT
VGGQEDMIDDTAMNMARERA
>Mature_340_residues
MNLTGKKVILHDMSLRDGMHARQHQISLKEMVDVATGLDAAGVPLIEVTHGDGLGGASLNYGFPAHTDEEYLSAVIPKMK
QAKVSALLLPGIGTGDHLRMAHDLGVNTIRVATHCTEADVSGQHISLSRELGLDTVGFLMMAHMVSPDKLLEQAKLMESY
GANCIYCTDSAGYMLPGDVTSHIERLRGELKSDTQIGFHGHHNMGMSIANSLAAIQAGAERIDGSVAGLGAGAGNTPLEV
FVAVLERMQVSHGINLYDIMDVAEDLVTPMMDQPIRIDRDALTLGYAGVYSSFLLFAQRAEKKHGIPARDILLELGRRGT
VGGQEDMIDDTAMNMARERA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1786548, Length=337, Percent_Identity=54.5994065281899, Blast_Score=369, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- ProDom:   PD005364 [H]

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 36540; Mature: 36540

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTGKKVILHDMSLRDGMHARQHQISLKEMVDVATGLDAAGVPLIEVTHGDGLGGASLN
CCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCC
YGFPAHTDEEYLSAVIPKMKQAKVSALLLPGIGTGDHLRMAHDLGVNTIRVATHCTEADV
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHCCCCEEEEEEECCCCCC
SGQHISLSRELGLDTVGFLMMAHMVSPDKLLEQAKLMESYGANCIYCTDSAGYMLPGDVT
CCCEEEEHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCEECCCHHH
SHIERLRGELKSDTQIGFHGHHNMGMSIANSLAAIQAGAERIDGSVAGLGAGAGNTPLEV
HHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHH
FVAVLERMQVSHGINLYDIMDVAEDLVTPMMDQPIRIDRDALTLGYAGVYSSFLLFAQRA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHH
EKKHGIPARDILLELGRRGTVGGQEDMIDDTAMNMARERA
HHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLTGKKVILHDMSLRDGMHARQHQISLKEMVDVATGLDAAGVPLIEVTHGDGLGGASLN
CCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCC
YGFPAHTDEEYLSAVIPKMKQAKVSALLLPGIGTGDHLRMAHDLGVNTIRVATHCTEADV
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHCCCCEEEEEEECCCCCC
SGQHISLSRELGLDTVGFLMMAHMVSPDKLLEQAKLMESYGANCIYCTDSAGYMLPGDVT
CCCEEEEHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCEECCCHHH
SHIERLRGELKSDTQIGFHGHHNMGMSIANSLAAIQAGAERIDGSVAGLGAGAGNTPLEV
HHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHH
FVAVLERMQVSHGINLYDIMDVAEDLVTPMMDQPIRIDRDALTLGYAGVYSSFLLFAQRA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHH
EKKHGIPARDILLELGRRGTVGGQEDMIDDTAMNMARERA
HHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA