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The map label for this gene is apbE [H]

Identifier: 157961911

GI number: 157961911

Start: 2530063

End: 2531346

Strand: Reverse

Name: apbE [H]

Synonym: Spea_2090

Alternate gene names: 157961911

Gene position: 2531346-2530063 (Counterclockwise)

Preceding gene: 157961915

Following gene: 157961910

Centisome position: 48.92

GC content: 37.46

Gene sequence:

>1284_bases
ATGAACATTATTCTTCATCAATATGATATTCATTTAATAATAACGACATTCTTAGGCTTTATTATGAAACGTATTTACCC
TTTATTGCTTTTACTAACAGCCACTAACGTTAGTCAAGCTGGTTTTGAAGAGCATCGCACTGAAACCCAAGTCTCTGTAA
ATAAAGCTGCGGTAACAGTACTTCCACCAGCACCAGATTTCGAAATCATTAAGGTTGATACTCAAGGTCAACCATATCGA
TGTGAAGGTAATGCCACAATTACTCTTGACCAAGGGGTCACAAATTTAACAACGTTAAAATATTTTGGCACCTCAATTAG
CATCAATATATATGACAAAAATAATTCGAATATAAATCTAGCACTTTGCAATGCACTAAAAGTGATACAGGAATACCATT
ACTTAGCATCAAACTACAGTACCTATCCCCATGTAATTAATATTAAGAGTATTAATAATGAGCCGGAAAAGACTCATCAT
CTGGATCCTAAGTTGACTCAACTGATTAAAGCCAGCATTGATTGGCATGATAAAACGGATGGTTACTTCAACATAGCCTT
GTCACCTGTGATCGATATCTGGCGAGGTTACAGGGCACAATGTAAAGGCGAGCGTAAATCACAAGACAAGTGTAATATCC
CGACGGATGCTGAACTTAATGCAGCCAAGCCACTCATTAACATCAATAATATTGATCTAGATGTAGCTAACAATACTATA
AAAATGCAAGCTGGTATGAGCATAGATCTGGGGGGGATTGCCAAAGGCTGGATGGCAGAGATGGTTTACCGCCAATTAAA
AAATGATGGTATAGACAATTTTATGATCAATGCAGGTGGCAACATTCGCCATTACGGGCTTCATCCACAAGGGCGCCAGT
TTACAACTGCAGTCGAAGATCCAGTGTGTAAGAAGTACAATAACTCACTCGAGCAATGCCAAAGTTTTGCAGGTCAATTT
CATGAAGTGATTACAGGTAAAGATATTACTGTGGTATCGAGCGGAAATTATCTAAGATACTATCGTGTTAATGGCCAAGA
ATACCATCATATTATTAATCCTAAAACCTTATACCCAAAGAAAGCGGGGATCTCGACCACCATAGTGATGAATGATAATC
AGATCTATGCAGACATAATTTCCACTGCATTGTTTTTAATGCCACTTAACGAGGCTATTAACTACACTAACCATAATAAC
GATGTCGAAGCGATATGGTATTTAAATGCAGCAGGTGATAAAAAAGAGAGTAACAACTTCAAAGATTACCGAATGAGTCT
CTAA

Upstream 100 bases:

>100_bases
GGTCAGAATAGAAGGTTTATAATTCTTCACAATTGCGGAAAACCGCAATACGTTAAGTTGCGTACTATTTATTTGACACA
GTTAACACTACCGATTATTG

Downstream 100 bases:

>100_bases
CTTTTCTTGTTCCACTCTACATTGAACTTGAATTTTTCACGGAACATGTGAATGAAATTTAAAAGTTTCGTGAAATACCG
AATATAAGTCGCAATCCCTG

Product: ApbE family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNIILHQYDIHLIITTFLGFIMKRIYPLLLLLTATNVSQAGFEEHRTETQVSVNKAAVTVLPPAPDFEIIKVDTQGQPYR
CEGNATITLDQGVTNLTTLKYFGTSISINIYDKNNSNINLALCNALKVIQEYHYLASNYSTYPHVINIKSINNEPEKTHH
LDPKLTQLIKASIDWHDKTDGYFNIALSPVIDIWRGYRAQCKGERKSQDKCNIPTDAELNAAKPLININNIDLDVANNTI
KMQAGMSIDLGGIAKGWMAEMVYRQLKNDGIDNFMINAGGNIRHYGLHPQGRQFTTAVEDPVCKKYNNSLEQCQSFAGQF
HEVITGKDITVVSSGNYLRYYRVNGQEYHHIINPKTLYPKKAGISTTIVMNDNQIYADIISTALFLMPLNEAINYTNHNN
DVEAIWYLNAAGDKKESNNFKDYRMSL

Sequences:

>Translated_427_residues
MNIILHQYDIHLIITTFLGFIMKRIYPLLLLLTATNVSQAGFEEHRTETQVSVNKAAVTVLPPAPDFEIIKVDTQGQPYR
CEGNATITLDQGVTNLTTLKYFGTSISINIYDKNNSNINLALCNALKVIQEYHYLASNYSTYPHVINIKSINNEPEKTHH
LDPKLTQLIKASIDWHDKTDGYFNIALSPVIDIWRGYRAQCKGERKSQDKCNIPTDAELNAAKPLININNIDLDVANNTI
KMQAGMSIDLGGIAKGWMAEMVYRQLKNDGIDNFMINAGGNIRHYGLHPQGRQFTTAVEDPVCKKYNNSLEQCQSFAGQF
HEVITGKDITVVSSGNYLRYYRVNGQEYHHIINPKTLYPKKAGISTTIVMNDNQIYADIISTALFLMPLNEAINYTNHNN
DVEAIWYLNAAGDKKESNNFKDYRMSL
>Mature_427_residues
MNIILHQYDIHLIITTFLGFIMKRIYPLLLLLTATNVSQAGFEEHRTETQVSVNKAAVTVLPPAPDFEIIKVDTQGQPYR
CEGNATITLDQGVTNLTTLKYFGTSISINIYDKNNSNINLALCNALKVIQEYHYLASNYSTYPHVINIKSINNEPEKTHH
LDPKLTQLIKASIDWHDKTDGYFNIALSPVIDIWRGYRAQCKGERKSQDKCNIPTDAELNAAKPLININNIDLDVANNTI
KMQAGMSIDLGGIAKGWMAEMVYRQLKNDGIDNFMINAGGNIRHYGLHPQGRQFTTAVEDPVCKKYNNSLEQCQSFAGQF
HEVITGKDITVVSSGNYLRYYRVNGQEYHHIINPKTLYPKKAGISTTIVMNDNQIYADIISTALFLMPLNEAINYTNHNN
DVEAIWYLNAAGDKKESNNFKDYRMSL

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=268, Percent_Identity=20.5223880597015, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 48248; Mature: 48248

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIILHQYDIHLIITTFLGFIMKRIYPLLLLLTATNVSQAGFEEHRTETQVSVNKAAVTV
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHEEEEEEEEEEE
LPPAPDFEIIKVDTQGQPYRCEGNATITLDQGVTNLTTLKYFGTSISINIYDKNNSNINL
ECCCCCEEEEEEECCCCCEEECCCEEEEECCCCCCEEEEEEECCEEEEEEEECCCCEEEE
ALCNALKVIQEYHYLASNYSTYPHVINIKSINNEPEKTHHLDPKLTQLIKASIDWHDKTD
EHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHCCCCCCCC
GYFNIALSPVIDIWRGYRAQCKGERKSQDKCNIPTDAELNAAKPLININNIDLDVANNTI
CEEEEEHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCEE
KMQAGMSIDLGGIAKGWMAEMVYRQLKNDGIDNFMINAGGNIRHYGLHPQGRQFTTAVED
EEECCCEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCEEEEHHCC
PVCKKYNNSLEQCQSFAGQFHEVITGKDITVVSSGNYLRYYRVNGQEYHHIINPKTLYPK
HHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCEEEEECCCCEECCC
KAGISTTIVMNDNQIYADIISTALFLMPLNEAINYTNHNNDVEAIWYLNAAGDKKESNNF
CCCCEEEEEEECCEEHHHHHHHHHHEEEHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCC
KDYRMSL
CCEEECC
>Mature Secondary Structure
MNIILHQYDIHLIITTFLGFIMKRIYPLLLLLTATNVSQAGFEEHRTETQVSVNKAAVTV
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHEEEEEEEEEEE
LPPAPDFEIIKVDTQGQPYRCEGNATITLDQGVTNLTTLKYFGTSISINIYDKNNSNINL
ECCCCCEEEEEEECCCCCEEECCCEEEEECCCCCCEEEEEEECCEEEEEEEECCCCEEEE
ALCNALKVIQEYHYLASNYSTYPHVINIKSINNEPEKTHHLDPKLTQLIKASIDWHDKTD
EHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHCCCCCCCC
GYFNIALSPVIDIWRGYRAQCKGERKSQDKCNIPTDAELNAAKPLININNIDLDVANNTI
CEEEEEHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCEE
KMQAGMSIDLGGIAKGWMAEMVYRQLKNDGIDNFMINAGGNIRHYGLHPQGRQFTTAVED
EEECCCEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCEEEEHHCC
PVCKKYNNSLEQCQSFAGQFHEVITGKDITVVSSGNYLRYYRVNGQEYHHIINPKTLYPK
HHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCEEEEECCCCEECCC
KAGISTTIVMNDNQIYADIISTALFLMPLNEAINYTNHNNDVEAIWYLNAAGDKKESNNF
CCCCEEEEEEECCEEHHHHHHHHHHEEEHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCC
KDYRMSL
CCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]