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The map label for this gene is fixL [H]

Identifier: 157961910

GI number: 157961910

Start: 2527932

End: 2529722

Strand: Reverse

Name: fixL [H]

Synonym: Spea_2089

Alternate gene names: 157961910

Gene position: 2529722-2527932 (Counterclockwise)

Preceding gene: 157961911

Following gene: 157961909

Centisome position: 48.89

GC content: 43.77

Gene sequence:

>1791_bases
ATGATGATATCCAAACTACGCCCTTTCTTAGCACTGAGTTGCCTTACTCTCTCTTGTTTCCAGGCATGCTATAGCTTTGC
TCAAGACTCAGTCCCAATTCGTGTCGGAGTGTTGGCATTTACTCATCCAGAGAACGTAGAAAAGCGCTGGCAACCGACAA
TAGATAGAATCGCAGCAGACTTAAACACGCCTTTTGAATTGGTCGCATTGACACCGAGCGAATTAGACGCCGATGTTGCA
CAGGGAAAATTAGATTTTTTAATCACTAATGCGTTAACCGGTGTTAGCTATAAAAAAGACTTTGGGACAAGTAGTCTTCT
CACACTGGTTCCTCGAGGTAATAAGCACCCTACGCAATCGGTAGGCTCAGCATTGATCATGCGGGCGCACGAGCAAGTTG
ATAATCTAGATGATTTAAAGACCCTCAGAGCAATTTCAACTGATAAACAAGCATTCGGTGGCTTTCAAATTTTTGCCGGC
GAAATGGCCCATAACGGTTTAAATCCCTTCAATGATTTTAAACAGTTGAATTTTGTTGGGTTTCCCCAGCAAAAGTTATT
GTCACTGATAGTTGACGGTAAAGCTGATATAGCAATTCTGCCCACTTGTGTGCTTGAGAATGCGATATTAAAAGGCGAGA
TTGCGGCTAAGGCCCTGCAAGTCGTGCTCGCTAAAGACGCACCTAGCTTTCAATGTCAAACCTCAAGCGAGCTATATCCC
TATTACTCTTTTTCCAAACTTGGCAAGACAGATCATCGCTTAGCAACTGAAGTCGTACGCTCGTTACTTTCAATAAAACC
CATAGATCATGCAGCAACACAAGGCCGCTATGACTCGTGGTCTGCCACGGTAAACGATAGCCATGTTTTTAAACTGCTGA
AACAGTTACAGCAATGGCCATTTGTCACCAATTGGACCTCTATTTTTAAGAGTGCACTGCCCTGGGCTGTTGTCGCCGCA
ATAATTTTGTTATTCGGTTATATTCATCACCTTAGAGTAAAGCGACTTGTAGTGCTGCGCACACGAGATCTTAAGGCAGA
AGTTGAACTACACACTCAAACACAAAAAGCCCTACTAGAGCAAACAAAGCAGTTTTATAAGGCTCAACGCGTCTTGTTGA
CTGGTGAAATGGCGTCAGGCATCGCCCATGAACTTAATCAACCCTTAGCAGGGATCCGTTATCTCACTCAGGGTTGTATT
TATCGCCTGTCTGATGATCAAGTCGAGCTTAAAGAGGCCATGAATAAAGCCATTCAGCAGGTCGATAGGGCTCAATCGAC
CATTAAGCGCTTTAGGCACTTCTGCCAACAACCTAGTGTGATGACCGAGTGTTGTTTAAATCAGGTTTTAGAAGATACGC
TCAGCCTAATGGCCGCCGAGTTTTCCCGAATACAGCTGACTCCAGACTTAAATAGCCTAGTGGTTAGCCTTAAAGCCGAT
CCGAGTCTTTTACAACAAGTATTCGTTAATATTATTCGTAATGCACTCGATGCCATGGCTCAAACTAAGGAGCCTTGTCT
ATCTATAACGCTCTCTAAGCAAGCTGAGCAGGCTTCTATTGTATTTAAAGATAATGGCACTGGTTTAAGCGAAACAGCCC
TAGAACGGTTATTCTTCCCTTTTGAAACATCAAAAGAGAACGGCTTAGGTCTTGGGATGATTATCTGTAAAAGGATCATC
GAAGAACATGACGGCGAGATCATCGCAGCTAACAATAACCTTGAAGGCTCGGCCGCTCCAGGTTTGACCATAACAATAAC
CCTACCTATTAAGGGGCCTCAAATTGTGTAA

Upstream 100 bases:

>100_bases
CCAAGTAAAATATGTGACATACTTCACCCGACGCCAGTTTTTACTACTACATTTATCTATAAGAAGGTCATAATAGCCAG
GTGTTTCTTTCACATACTCA

Downstream 100 bases:

>100_bases
CTTATATCTAGTCGACGACGATCAAGCGGTATTAGACTCTTTGACTTGGATGTTAAATGGGCTTGGCTTCCAACCCCAAG
GATTCCTGTCCGCCGACAGT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MMISKLRPFLALSCLTLSCFQACYSFAQDSVPIRVGVLAFTHPENVEKRWQPTIDRIAADLNTPFELVALTPSELDADVA
QGKLDFLITNALTGVSYKKDFGTSSLLTLVPRGNKHPTQSVGSALIMRAHEQVDNLDDLKTLRAISTDKQAFGGFQIFAG
EMAHNGLNPFNDFKQLNFVGFPQQKLLSLIVDGKADIAILPTCVLENAILKGEIAAKALQVVLAKDAPSFQCQTSSELYP
YYSFSKLGKTDHRLATEVVRSLLSIKPIDHAATQGRYDSWSATVNDSHVFKLLKQLQQWPFVTNWTSIFKSALPWAVVAA
IILLFGYIHHLRVKRLVVLRTRDLKAEVELHTQTQKALLEQTKQFYKAQRVLLTGEMASGIAHELNQPLAGIRYLTQGCI
YRLSDDQVELKEAMNKAIQQVDRAQSTIKRFRHFCQQPSVMTECCLNQVLEDTLSLMAAEFSRIQLTPDLNSLVVSLKAD
PSLLQQVFVNIIRNALDAMAQTKEPCLSITLSKQAEQASIVFKDNGTGLSETALERLFFPFETSKENGLGLGMIICKRII
EEHDGEIIAANNNLEGSAAPGLTITITLPIKGPQIV

Sequences:

>Translated_596_residues
MMISKLRPFLALSCLTLSCFQACYSFAQDSVPIRVGVLAFTHPENVEKRWQPTIDRIAADLNTPFELVALTPSELDADVA
QGKLDFLITNALTGVSYKKDFGTSSLLTLVPRGNKHPTQSVGSALIMRAHEQVDNLDDLKTLRAISTDKQAFGGFQIFAG
EMAHNGLNPFNDFKQLNFVGFPQQKLLSLIVDGKADIAILPTCVLENAILKGEIAAKALQVVLAKDAPSFQCQTSSELYP
YYSFSKLGKTDHRLATEVVRSLLSIKPIDHAATQGRYDSWSATVNDSHVFKLLKQLQQWPFVTNWTSIFKSALPWAVVAA
IILLFGYIHHLRVKRLVVLRTRDLKAEVELHTQTQKALLEQTKQFYKAQRVLLTGEMASGIAHELNQPLAGIRYLTQGCI
YRLSDDQVELKEAMNKAIQQVDRAQSTIKRFRHFCQQPSVMTECCLNQVLEDTLSLMAAEFSRIQLTPDLNSLVVSLKAD
PSLLQQVFVNIIRNALDAMAQTKEPCLSITLSKQAEQASIVFKDNGTGLSETALERLFFPFETSKENGLGLGMIICKRII
EEHDGEIIAANNNLEGSAAPGLTITITLPIKGPQIV
>Mature_596_residues
MMISKLRPFLALSCLTLSCFQACYSFAQDSVPIRVGVLAFTHPENVEKRWQPTIDRIAADLNTPFELVALTPSELDADVA
QGKLDFLITNALTGVSYKKDFGTSSLLTLVPRGNKHPTQSVGSALIMRAHEQVDNLDDLKTLRAISTDKQAFGGFQIFAG
EMAHNGLNPFNDFKQLNFVGFPQQKLLSLIVDGKADIAILPTCVLENAILKGEIAAKALQVVLAKDAPSFQCQTSSELYP
YYSFSKLGKTDHRLATEVVRSLLSIKPIDHAATQGRYDSWSATVNDSHVFKLLKQLQQWPFVTNWTSIFKSALPWAVVAA
IILLFGYIHHLRVKRLVVLRTRDLKAEVELHTQTQKALLEQTKQFYKAQRVLLTGEMASGIAHELNQPLAGIRYLTQGCI
YRLSDDQVELKEAMNKAIQQVDRAQSTIKRFRHFCQQPSVMTECCLNQVLEDTLSLMAAEFSRIQLTPDLNSLVVSLKAD
PSLLQQVFVNIIRNALDAMAQTKEPCLSITLSKQAEQASIVFKDNGTGLSETALERLFFPFETSKENGLGLGMIICKRII
EEHDGEIIAANNNLEGSAAPGLTITITLPIKGPQIV

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=238, Percent_Identity=30.672268907563, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790436, Length=298, Percent_Identity=25.8389261744966, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790300, Length=240, Percent_Identity=29.1666666666667, Blast_Score=93, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 66073; Mature: 66073

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMISKLRPFLALSCLTLSCFQACYSFAQDSVPIRVGVLAFTHPENVEKRWQPTIDRIAAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHCCHHHHHHHHH
LNTPFELVALTPSELDADVAQGKLDFLITNALTGVSYKKDFGTSSLLTLVPRGNKHPTQS
CCCCEEEEEECHHHHCHHHHCCHHHEEEHHHHCCCCCHHCCCCCCEEEEECCCCCCCHHH
VGSALIMRAHEQVDNLDDLKTLRAISTDKQAFGGFQIFAGEMAHNGLNPFNDFKQLNFVG
HHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHCCHHEEEHHHHHCCCCCHHHHHHCCCCC
FPQQKLLSLIVDGKADIAILPTCVLENAILKGEIAAKALQVVLAKDAPSFQCQTSSELYP
CCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCC
YYSFSKLGKTDHRLATEVVRSLLSIKPIDHAATQGRYDSWSATVNDSHVFKLLKQLQQWP
CCCHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
FVTNWTSIFKSALPWAVVAAIILLFGYIHHLRVKRLVVLRTRDLKAEVELHTQTQKALLE
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHH
QTKQFYKAQRVLLTGEMASGIAHELNQPLAGIRYLTQGCIYRLSDDQVELKEAMNKAIQQ
HHHHHHHHHHHHEECHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHH
VDRAQSTIKRFRHFCQQPSVMTECCLNQVLEDTLSLMAAEFSRIQLTPDLNSLVVSLKAD
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHEEEECCC
PSLLQQVFVNIIRNALDAMAQTKEPCLSITLSKQAEQASIVFKDNGTGLSETALERLFFP
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCC
FETSKENGLGLGMIICKRIIEEHDGEIIAANNNLEGSAAPGLTITITLPIKGPQIV
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MMISKLRPFLALSCLTLSCFQACYSFAQDSVPIRVGVLAFTHPENVEKRWQPTIDRIAAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHCCHHHHHHHHH
LNTPFELVALTPSELDADVAQGKLDFLITNALTGVSYKKDFGTSSLLTLVPRGNKHPTQS
CCCCEEEEEECHHHHCHHHHCCHHHEEEHHHHCCCCCHHCCCCCCEEEEECCCCCCCHHH
VGSALIMRAHEQVDNLDDLKTLRAISTDKQAFGGFQIFAGEMAHNGLNPFNDFKQLNFVG
HHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHCCHHEEEHHHHHCCCCCHHHHHHCCCCC
FPQQKLLSLIVDGKADIAILPTCVLENAILKGEIAAKALQVVLAKDAPSFQCQTSSELYP
CCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCC
YYSFSKLGKTDHRLATEVVRSLLSIKPIDHAATQGRYDSWSATVNDSHVFKLLKQLQQWP
CCCHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
FVTNWTSIFKSALPWAVVAAIILLFGYIHHLRVKRLVVLRTRDLKAEVELHTQTQKALLE
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHH
QTKQFYKAQRVLLTGEMASGIAHELNQPLAGIRYLTQGCIYRLSDDQVELKEAMNKAIQQ
HHHHHHHHHHHHEECHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHH
VDRAQSTIKRFRHFCQQPSVMTECCLNQVLEDTLSLMAAEFSRIQLTPDLNSLVVSLKAD
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHEEEECCC
PSLLQQVFVNIIRNALDAMAQTKEPCLSITLSKQAEQASIVFKDNGTGLSETALERLFFP
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCC
FETSKENGLGLGMIICKRIIEEHDGEIIAANNNLEGSAAPGLTITITLPIKGPQIV
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]