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The map label for this gene is gyrA [H]

Identifier: 157961888

GI number: 157961888

Start: 2503066

End: 2505777

Strand: Direct

Name: gyrA [H]

Synonym: Spea_2067

Alternate gene names: 157961888

Gene position: 2503066-2505777 (Clockwise)

Preceding gene: 157961881

Following gene: 157961890

Centisome position: 48.37

GC content: 44.87

Gene sequence:

>2712_bases
ATGACTGATCTGGCTTCATCTATAACACCAATTAATATTGAAGACGAATTAAAAAATTCATACTTAGATTACGCCATGAG
CGTAATCGTTGGCCGTGCACTGCCTGACGTTCGTGACGGTTTAAAACCGGTACATCGCCGCGTACTTTTCGCCATGAGCG
AATTGAAAAACGACTGGAACAAGCCTTATAAAAAGTCTGCCCGTGTCGTTGGTGACGTAATCGGTAAGTATCACCCGCAT
GGTGATACCGCTGTATATGACACAATCGTTCGTTTGGCTCAGCCTTTCTCTATGCGTTACCCACTTATTGACGGTCAAGG
TAACTTCGGTTCTGTCGATGGCGACGCCGCAGCTGCAATGCGTTATACCGAAATCCGTATGGACAAGGTGGCACATCAGC
TTTTAGCCGACCTTGAGAAAGAGACCGTTGACTTCGTTCCAAACTACGATGGCACCGAGTTTATTCCTGCCGTTCTACCA
ACACGCATTCCAACACTACTAGTGAATGGTTCGTCAGGTATTGCGGTTGGTATGGCTACAAACATTCCGCCACATAACTT
GAATGAAGTTATCAGCGGTTGTTTAGCACTAATCGAAGACCCTGCATTATCTATCGAACAATTGATGGAATACATTCCAG
GTCCTGACTTCCCAACTGCAGCGATTATTAATGGCCGTAAAGGCATTATCGATGCGTATAAAACCGGCCGTGGCCGTGCG
GTTATGCGTGCTCGTGCAGAGATCGAAGAAGAGAATGGCCGCGAGCGAATTATCGTTCACGAAATTCCATATCAGGTTAA
CAAAGCGCGTTTGATTGAAAAAATTGCCGAGCTAGTTAAAGATAAGAAGATCGAAGGTATTAGTGGTCTACGCGACGAGT
CTGATAAAGACGGCATGCGTATTGTTATCGAGATCAAGCGTGGTGAAGTGGGCGAGGTTGTTCTAAATAACCTTTACGCA
CAAACTCAAATGCAGTGCTCTTTTGGTATTAACATGGTTGCTCTGACCAATGGTCAGCCAAAACTATTTAATTTAAAAGA
GATGCTAGAGTGCTTTATTCTTCACCGTCGTGAAGTGGTTACTCGTCGTACCGTATTTGAATTACGTAAAGCACGTGAAC
GCGCGCATATTCTTGAAGCGTTAGCGGTGGCGCTTGCAAACATCGACCCAATTATCGCCTTGATTAAGGCATCTCCGACT
CCAGCCGAAGCGAAAGTGAAGCTAGTTGCAGAAGGTTGGGAACTTGGCCACGTTAAAGGCATGCTTGAAAAAGCAGGCGA
TGACGCAGCCCGTCCTGAGTGGCTAGAGCCTGAATTTGGTATTCGCGACGATAAGTACTATCTGACTGAGCAACAAGCTC
AAGCGATCCTTGAACTACGTTTACACCGTTTAACCGGTCTAGAACATGACAAGATCATTGCTGAATACGAAGAACTACTA
GAAATCATCGCGGCATTACTATTCATTCTTGGTAGCCCAGAGCGTTTGATGGAAGTGATTAAAGAAGAACTTGAAGAAGT
TCTGACAAACTTTGGTGACGAGCGCCGTACTGTTATTAACGCTAACGAAGTTGATATGAGTCTAGAAGATCTTATCAATG
AAGAAGACGTTGTAGTTACACTGTCGCACCTTGGTTATGCCAAGTACCAAGTTCTATCTGATTATCAGGCTCAACGCCGT
GGTGGTAAGGGTAAAGCTGCAACTAAAGTGAAAGACGAAGATTTCGTTGAAAAACTATTGGTAGCTAACACTCATGACAC
CATCTTGTGCTTCTCTGACTTTGGTAAGATGTACTGGCTGAAAGTTTACCAATTGCCATTGGCAAGCCGTACAGCACGTG
GTCGCCCAATTGTTAACTTGTTACCGTTGGCAGAAGGTGAGCGTATCACTGCGATTCTACCTGTTCGCGAATACGCTGAA
GACAAGTACATCATTATGGCGACGTCTCACGGTACAGTTAAGAAGACTGCACTAACGGCTTATAGCAACCCACGTGTAAA
CGGTATTATCGCTGTAAACCTTAAAGATGGTGACCAGTTGATTGGTGTTGATATTACCGATGGTAATGACGATATCATGC
TGTTCTCGAATGAAGGTAAAGTGGTTCGCTTTAACGAGAAAGCCCGTAGCTCTGAAACAGGTGAAGTGAAAATTGATCCT
GAAACAGGCGAAGAAGTTATCGCACTACGTGCGATGGGACGTACTGCAACGGGTGTTCGTGGTATTAAGCTTGAAGATGG
CCAAACTGTTGTATCGCTAATTGTACCTAAGGGTGAGGGTGATATCTTAACTGTGACCGAGAACGGTTATGGTAAGCGTA
CCGATTTAGCCGAATATCCAGCTAAGAGCCGTGGTACGAAAGGTGTTGTCTCTATCAAAGTGAGCGAACGTAACGGCGCC
GTAGTTGGTGCAGTACAAGTTGGCGAAAATGATGAGATCATGCTGATCAGTAACAAGGGTACTTTAGTACGTACTCCAGC
TACTGGCGTATCAAGCATTGGTCGTAACACTCAAGGTGTGACCATTATTCGTACCGCTGAAGATGAGAAAGTTGTTGGTC
TACAACGTATCGATGAAATTCAAGTTCCTGATGTTGAGCTTGATGAAGAGGGTAACCCTATCATCGTTGAAGGCGCTGCA
GGAACAGAAGTTCAAGCAGAAGCTGTCAATACCGAAGCTGAAGTGACTGAAACACCAAAAATTGAAGATTAA

Upstream 100 bases:

>100_bases
GCCATAAAATAACTGTGTTAGAATGCCAAATCACGTTTTCAAGCATAGCTATTTTAGGAAATAAGAATAGCACGCTAAAT
TTCAGGGGATCGAGCAGTTT

Downstream 100 bases:

>100_bases
TATTCATCAATGAGATGATGTATTGATTTGAAAAAAAGGATGCCAATGGCATCCTTTTTTGTTTTTAGCCCTAAACCACT
TCAGTAGGTGGTTATCATCA

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 903; Mature: 902

Protein sequence:

>903_residues
MTDLASSITPINIEDELKNSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMSELKNDWNKPYKKSARVVGDVIGKYHPH
GDTAVYDTIVRLAQPFSMRYPLIDGQGNFGSVDGDAAAAMRYTEIRMDKVAHQLLADLEKETVDFVPNYDGTEFIPAVLP
TRIPTLLVNGSSGIAVGMATNIPPHNLNEVISGCLALIEDPALSIEQLMEYIPGPDFPTAAIINGRKGIIDAYKTGRGRA
VMRARAEIEEENGRERIIVHEIPYQVNKARLIEKIAELVKDKKIEGISGLRDESDKDGMRIVIEIKRGEVGEVVLNNLYA
QTQMQCSFGINMVALTNGQPKLFNLKEMLECFILHRREVVTRRTVFELRKARERAHILEALAVALANIDPIIALIKASPT
PAEAKVKLVAEGWELGHVKGMLEKAGDDAARPEWLEPEFGIRDDKYYLTEQQAQAILELRLHRLTGLEHDKIIAEYEELL
EIIAALLFILGSPERLMEVIKEELEEVLTNFGDERRTVINANEVDMSLEDLINEEDVVVTLSHLGYAKYQVLSDYQAQRR
GGKGKAATKVKDEDFVEKLLVANTHDTILCFSDFGKMYWLKVYQLPLASRTARGRPIVNLLPLAEGERITAILPVREYAE
DKYIIMATSHGTVKKTALTAYSNPRVNGIIAVNLKDGDQLIGVDITDGNDDIMLFSNEGKVVRFNEKARSSETGEVKIDP
ETGEEVIALRAMGRTATGVRGIKLEDGQTVVSLIVPKGEGDILTVTENGYGKRTDLAEYPAKSRGTKGVVSIKVSERNGA
VVGAVQVGENDEIMLISNKGTLVRTPATGVSSIGRNTQGVTIIRTAEDEKVVGLQRIDEIQVPDVELDEEGNPIIVEGAA
GTEVQAEAVNTEAEVTETPKIED

Sequences:

>Translated_903_residues
MTDLASSITPINIEDELKNSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMSELKNDWNKPYKKSARVVGDVIGKYHPH
GDTAVYDTIVRLAQPFSMRYPLIDGQGNFGSVDGDAAAAMRYTEIRMDKVAHQLLADLEKETVDFVPNYDGTEFIPAVLP
TRIPTLLVNGSSGIAVGMATNIPPHNLNEVISGCLALIEDPALSIEQLMEYIPGPDFPTAAIINGRKGIIDAYKTGRGRA
VMRARAEIEEENGRERIIVHEIPYQVNKARLIEKIAELVKDKKIEGISGLRDESDKDGMRIVIEIKRGEVGEVVLNNLYA
QTQMQCSFGINMVALTNGQPKLFNLKEMLECFILHRREVVTRRTVFELRKARERAHILEALAVALANIDPIIALIKASPT
PAEAKVKLVAEGWELGHVKGMLEKAGDDAARPEWLEPEFGIRDDKYYLTEQQAQAILELRLHRLTGLEHDKIIAEYEELL
EIIAALLFILGSPERLMEVIKEELEEVLTNFGDERRTVINANEVDMSLEDLINEEDVVVTLSHLGYAKYQVLSDYQAQRR
GGKGKAATKVKDEDFVEKLLVANTHDTILCFSDFGKMYWLKVYQLPLASRTARGRPIVNLLPLAEGERITAILPVREYAE
DKYIIMATSHGTVKKTALTAYSNPRVNGIIAVNLKDGDQLIGVDITDGNDDIMLFSNEGKVVRFNEKARSSETGEVKIDP
ETGEEVIALRAMGRTATGVRGIKLEDGQTVVSLIVPKGEGDILTVTENGYGKRTDLAEYPAKSRGTKGVVSIKVSERNGA
VVGAVQVGENDEIMLISNKGTLVRTPATGVSSIGRNTQGVTIIRTAEDEKVVGLQRIDEIQVPDVELDEEGNPIIVEGAA
GTEVQAEAVNTEAEVTETPKIED
>Mature_902_residues
TDLASSITPINIEDELKNSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMSELKNDWNKPYKKSARVVGDVIGKYHPHG
DTAVYDTIVRLAQPFSMRYPLIDGQGNFGSVDGDAAAAMRYTEIRMDKVAHQLLADLEKETVDFVPNYDGTEFIPAVLPT
RIPTLLVNGSSGIAVGMATNIPPHNLNEVISGCLALIEDPALSIEQLMEYIPGPDFPTAAIINGRKGIIDAYKTGRGRAV
MRARAEIEEENGRERIIVHEIPYQVNKARLIEKIAELVKDKKIEGISGLRDESDKDGMRIVIEIKRGEVGEVVLNNLYAQ
TQMQCSFGINMVALTNGQPKLFNLKEMLECFILHRREVVTRRTVFELRKARERAHILEALAVALANIDPIIALIKASPTP
AEAKVKLVAEGWELGHVKGMLEKAGDDAARPEWLEPEFGIRDDKYYLTEQQAQAILELRLHRLTGLEHDKIIAEYEELLE
IIAALLFILGSPERLMEVIKEELEEVLTNFGDERRTVINANEVDMSLEDLINEEDVVVTLSHLGYAKYQVLSDYQAQRRG
GKGKAATKVKDEDFVEKLLVANTHDTILCFSDFGKMYWLKVYQLPLASRTARGRPIVNLLPLAEGERITAILPVREYAED
KYIIMATSHGTVKKTALTAYSNPRVNGIIAVNLKDGDQLIGVDITDGNDDIMLFSNEGKVVRFNEKARSSETGEVKIDPE
TGEEVIALRAMGRTATGVRGIKLEDGQTVVSLIVPKGEGDILTVTENGYGKRTDLAEYPAKSRGTKGVVSIKVSERNGAV
VGAVQVGENDEIMLISNKGTLVRTPATGVSSIGRNTQGVTIIRTAEDEKVVGLQRIDEIQVPDVELDEEGNPIIVEGAAG
TEVQAEAVNTEAEVTETPKIED

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=175, Percent_Identity=32, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI19913408, Length=179, Percent_Identity=32.4022346368715, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1788562, Length=861, Percent_Identity=72.1254355400697, Blast_Score=1272, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=717, Percent_Identity=36.6806136680614, Blast_Score=408, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17535065, Length=174, Percent_Identity=33.9080459770115, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI212645657, Length=173, Percent_Identity=33.5260115606936, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI212645845, Length=368, Percent_Identity=24.7282608695652, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=346, Percent_Identity=27.1676300578035, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17136538, Length=349, Percent_Identity=25.7879656160458, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 99657; Mature: 99526

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLASSITPINIEDELKNSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMSELKNDWN
CCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
KPYKKSARVVGDVIGKYHPHGDTAVYDTIVRLAQPFSMRYPLIDGQGNFGSVDGDAAAAM
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCHHHHH
RYTEIRMDKVAHQLLADLEKETVDFVPNYDGTEFIPAVLPTRIPTLLVNGSSGIAVGMAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCCEEEEEEC
NIPPHNLNEVISGCLALIEDPALSIEQLMEYIPGPDFPTAAIINGRKGIIDAYKTGRGRA
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEECCCCCCHHHHHCCCCCE
VMRARAEIEEENGRERIIVHEIPYQVNKARLIEKIAELVKDKKIEGISGLRDESDKDGMR
EHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
IVIEIKRGEVGEVVLNNLYAQTQMQCSFGINMVALTNGQPKLFNLKEMLECFILHRREVV
EEEEECCCCHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHH
TRRTVFELRKARERAHILEALAVALANIDPIIALIKASPTPAEAKVKLVAEGWELGHVKG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHH
MLEKAGDDAARPEWLEPEFGIRDDKYYLTEQQAQAILELRLHRLTGLEHDKIIAEYEELL
HHHHCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EIIAALLFILGSPERLMEVIKEELEEVLTNFGDERRTVINANEVDMSLEDLINEEDVVVT
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHCCCCCEEEE
LSHLGYAKYQVLSDYQAQRRGGKGKAATKVKDEDFVEKLLVANTHDTILCFSDFGKMYWL
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEE
KVYQLPLASRTARGRPIVNLLPLAEGERITAILPVREYAEDKYIIMATSHGTVKKTALTA
HEEECCCCCCCCCCCCCEEEEECCCCCEEEEEECHHHHCCCCEEEEEECCCCEEEEEEEE
YSNPRVNGIIAVNLKDGDQLIGVDITDGNDDIMLFSNEGKVVRFNEKARSSETGEVKIDP
CCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEEECCCCEEEECCCCCCCCCCEEEECC
ETGEEVIALRAMGRTATGVRGIKLEDGQTVVSLIVPKGEGDILTVTENGYGKRTDLAEYP
CCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCEEEEECCCCCCCCCHHHCC
AKSRGTKGVVSIKVSERNGAVVGAVQVGENDEIMLISNKGTLVRTPATGVSSIGRNTQGV
CCCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEEECCCCEEECCCCCHHHHCCCCCCE
TIIRTAEDEKVVGLQRIDEIQVPDVELDEEGNPIIVEGAAGTEVQAEAVNTEAEVTETPK
EEEEECCCCCEECHHHHCCCCCCCCEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCCC
IED
CCC
>Mature Secondary Structure 
TDLASSITPINIEDELKNSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMSELKNDWN
CCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
KPYKKSARVVGDVIGKYHPHGDTAVYDTIVRLAQPFSMRYPLIDGQGNFGSVDGDAAAAM
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCHHHHH
RYTEIRMDKVAHQLLADLEKETVDFVPNYDGTEFIPAVLPTRIPTLLVNGSSGIAVGMAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCCEEEEEEC
NIPPHNLNEVISGCLALIEDPALSIEQLMEYIPGPDFPTAAIINGRKGIIDAYKTGRGRA
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEECCCCCCHHHHHCCCCCE
VMRARAEIEEENGRERIIVHEIPYQVNKARLIEKIAELVKDKKIEGISGLRDESDKDGMR
EHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
IVIEIKRGEVGEVVLNNLYAQTQMQCSFGINMVALTNGQPKLFNLKEMLECFILHRREVV
EEEEECCCCHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHH
TRRTVFELRKARERAHILEALAVALANIDPIIALIKASPTPAEAKVKLVAEGWELGHVKG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHH
MLEKAGDDAARPEWLEPEFGIRDDKYYLTEQQAQAILELRLHRLTGLEHDKIIAEYEELL
HHHHCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EIIAALLFILGSPERLMEVIKEELEEVLTNFGDERRTVINANEVDMSLEDLINEEDVVVT
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHCCCCCEEEE
LSHLGYAKYQVLSDYQAQRRGGKGKAATKVKDEDFVEKLLVANTHDTILCFSDFGKMYWL
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEE
KVYQLPLASRTARGRPIVNLLPLAEGERITAILPVREYAEDKYIIMATSHGTVKKTALTA
HEEECCCCCCCCCCCCCEEEEECCCCCEEEEEECHHHHCCCCEEEEEECCCCEEEEEEEE
YSNPRVNGIIAVNLKDGDQLIGVDITDGNDDIMLFSNEGKVVRFNEKARSSETGEVKIDP
CCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEEECCCCEEEECCCCCCCCCCEEEECC
ETGEEVIALRAMGRTATGVRGIKLEDGQTVVSLIVPKGEGDILTVTENGYGKRTDLAEYP
CCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCEEEEECCCCCCCCCHHHCC
AKSRGTKGVVSIKVSERNGAVVGAVQVGENDEIMLISNKGTLVRTPATGVSSIGRNTQGV
CCCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEEECCCCEEECCCCCHHHHCCCCCCE
TIIRTAEDEKVVGLQRIDEIQVPDVELDEEGNPIIVEGAAGTEVQAEAVNTEAEVTETPK
EEEEECCCCCEECHHHHCCCCCCCCEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCCC
IED
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7642123 [H]