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The map label for this gene is nrdA [H]

Identifier: 157961885

GI number: 157961885

Start: 2498658

End: 2500946

Strand: Reverse

Name: nrdA [H]

Synonym: Spea_2064

Alternate gene names: 157961885

Gene position: 2500946-2498658 (Counterclockwise)

Preceding gene: 157961886

Following gene: 157961884

Centisome position: 48.33

GC content: 43.56

Gene sequence:

>2289_bases
ATGAATAGCAATATGACAGTCACAAAGCGTAGTGGCGAACGTGAGACTATCGATCTCGATAAAATCCATCGCGTTATCGA
GTGGGCGGCAAAAGGACTAAACAACGTCTCTGTATCTGAAGTTGAACTGCGCTCTCACCTGCAGTTTTTTGACGGCATTG
CCACTGAAGCCATCCATGAGACTATCATTAAAGCTGCTGCAGATTTAATCTCTCCAGAATCACCAGATTATCAGTTCCTG
TCTGCACGCTTAGCTGTTTTCCATCTTCGTAAGAAAGCTTTTGGTCAATTTGAGCCACCTAAGCTTTACGATCATGTGGT
TAAGTTGATAGAGCTTGGTAAGTACGATCAGCATATTCTAGAAGACTACAGTCAAGAAGAGATCGATACCCTAGATAGCT
ATATCGATCACTGGCGTGATATGAACTTCTCTTATGCTGCAGTTAAGCAACTTGAAGGTAAATACCTAGTTCAAAACCGT
GTAACCCACGAGATCTACGAAAGTGCCCAGTTCCTGTATATCTTGGTTGCTGCTTGCCTATTTGCTAAGTATCCAAAAGA
GACGCGCCTTCAGTACGTTAAAGATTTCTACGACGCGACATCTACATTTAAGATCTCGCTACCAACACCTATCATGGCCG
GTGTACGTACGCCTACACGTCAGTTCAGCTCATGTGTATTGATTGAGTGTGGTGACAGCCTTGATTCAATTAATGCTACC
TCATCGTCAATCGTTAAGTACGTGTCACAACGTGCTGGTATCGGCGTCAATGCTGGCCGTATTCGTGCCCTAGGTAGTCC
AATCCGTGGTGGTGAAGCATTCCATACAGGTTGTTTACCATTTTATAAGTATTTCCAGACTGCGGTTAAATCTTGTTCTC
AAGGTGGCGTACGTGGTGGTGCAGCTACCCTATTCTACCCAATGTGGCACCTAGAAGTTGAATCACTGTTAGTACTTAAG
AACAACCGTGGTGTTGATGATAACCGTATCCGTCATTTAGATTACGGCGTTCAAATCAACAAAGTTATGTATCAACGTCT
TATTCAAGGCGGCATGATCAGCTTGTTCAGCCCATCAGATGTACCAGGTATGTACGATGCATTCTTCGAGGACCAAGAAG
AATTCGAGCGTCTGTATCTTAAATATGAAGCTGATGACAGTATTCGTAAGAAACAGATTAAAGCCGTTGAGTTATTCTCG
TTAATGATGCAAGAGCGCGCCTCTACCGGTCGTATCTACATTCAAAACGTTGACCACTGTAATACCCACAGTCCATTTGA
CTCTAAGGTTGCACCAGTTCGTCAGTCTAACTTGTGCCTTGAGATTGCATTGCCAACTAAGCCGTTAAACAACATTAACG
ATCCTGATGGCGAGATTGCACTTTGTACACTGTCAGCACTTAACTTAGGTGCTATCAAGAAGTTAGAAGAATTAGAGCCA
TTGGCCGATCTTGCTGTACGTGCACTCGATAACCTACTGGATTATCAAGATTACCCAATCATCTCAGCTCAAAAAGCGTC
AATGAATCGCCGCACACTGGGTATAGGCGTAATTAACTTTGCTAACTACTTAGCAAAAGAAGGCGTACGTTACTCAGACG
GCTCAGCGAACGGCATTACTCACAAGACTTTTGAAGCGATTCAATTCTACCTATTGAAGGCATCGATGAAGCTGGCTAAG
GAACAAGGCGCCTGCCCTGCGTTCCATGAGACAACTTACTCTAAAGGTATTTTACCAATTGATACCTACAAGCGTGATTT
AGATAAGATTTGCGATGAGCCATTGCACCTAGATTGGGAAACGTTGCGTGAAGAGATCAAGACTCACGGTCTACGTAACT
CTACCCTTTCTGCGCTAATGCCATCAGAGACCTCTTCGCAGATCTCAAACGCAACTAACGGTATTGAGCCACCACGTGGT
TTAATCAGCGTTAAAGCCAGTAAAGATGGTCAGCTAAAGCAAGTAGTACCTGACTTTGAAAAGTACCAGTATAGCTATGA
GTTACTATGGCAAATGCCTAATAACGATGGTTACTTACAGTTAGTCGGTTTAATGCAGAAGTTCGTCGATCAGTCGATTT
CAGCTAACACCAACTACGACCCTAGTCGTTTTGAGGGTGGCCGAGTACCGATGAAGGTACTATTAAAAGACTTATTAACC
GCTTACAAATATGGTGTGAAGACACTTTACTATCACAATACTCGTGATGGAGCTTCAGATAGTCATGACGATATCACTGC
CATCGAGAAAGAAGATGACAGTTGCGCTGGCGGCGCATGTAAGATCTAA

Upstream 100 bases:

>100_bases
AACTCTCCGTTTTTCGGTTAAAACTGCTTGTTTACAAGGAAACGTACGGTGCACCTGAACGGGTCACTTTCATAAATATA
AAAACCAAGGTTTATCTTTA

Downstream 100 bases:

>100_bases
CCCAAGATATTAATCATTAGTATTAAACGTTAATTTAAGGGGGCCTAGCCCCCATTATCGGAAGATAGAAAAATGGCCTA
TTCAACATTTTGTCAAACTC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Protein B1; Ribonucleoside-diphosphate reductase 1 R1 subunit; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 762; Mature: 762

Protein sequence:

>762_residues
MNSNMTVTKRSGERETIDLDKIHRVIEWAAKGLNNVSVSEVELRSHLQFFDGIATEAIHETIIKAAADLISPESPDYQFL
SARLAVFHLRKKAFGQFEPPKLYDHVVKLIELGKYDQHILEDYSQEEIDTLDSYIDHWRDMNFSYAAVKQLEGKYLVQNR
VTHEIYESAQFLYILVAACLFAKYPKETRLQYVKDFYDATSTFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
SSSIVKYVSQRAGIGVNAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLK
NNRGVDDNRIRHLDYGVQINKVMYQRLIQGGMISLFSPSDVPGMYDAFFEDQEEFERLYLKYEADDSIRKKQIKAVELFS
LMMQERASTGRIYIQNVDHCNTHSPFDSKVAPVRQSNLCLEIALPTKPLNNINDPDGEIALCTLSALNLGAIKKLEELEP
LADLAVRALDNLLDYQDYPIISAQKASMNRRTLGIGVINFANYLAKEGVRYSDGSANGITHKTFEAIQFYLLKASMKLAK
EQGACPAFHETTYSKGILPIDTYKRDLDKICDEPLHLDWETLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRG
LISVKASKDGQLKQVVPDFEKYQYSYELLWQMPNNDGYLQLVGLMQKFVDQSISANTNYDPSRFEGGRVPMKVLLKDLLT
AYKYGVKTLYYHNTRDGASDSHDDITAIEKEDDSCAGGACKI

Sequences:

>Translated_762_residues
MNSNMTVTKRSGERETIDLDKIHRVIEWAAKGLNNVSVSEVELRSHLQFFDGIATEAIHETIIKAAADLISPESPDYQFL
SARLAVFHLRKKAFGQFEPPKLYDHVVKLIELGKYDQHILEDYSQEEIDTLDSYIDHWRDMNFSYAAVKQLEGKYLVQNR
VTHEIYESAQFLYILVAACLFAKYPKETRLQYVKDFYDATSTFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
SSSIVKYVSQRAGIGVNAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLK
NNRGVDDNRIRHLDYGVQINKVMYQRLIQGGMISLFSPSDVPGMYDAFFEDQEEFERLYLKYEADDSIRKKQIKAVELFS
LMMQERASTGRIYIQNVDHCNTHSPFDSKVAPVRQSNLCLEIALPTKPLNNINDPDGEIALCTLSALNLGAIKKLEELEP
LADLAVRALDNLLDYQDYPIISAQKASMNRRTLGIGVINFANYLAKEGVRYSDGSANGITHKTFEAIQFYLLKASMKLAK
EQGACPAFHETTYSKGILPIDTYKRDLDKICDEPLHLDWETLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRG
LISVKASKDGQLKQVVPDFEKYQYSYELLWQMPNNDGYLQLVGLMQKFVDQSISANTNYDPSRFEGGRVPMKVLLKDLLT
AYKYGVKTLYYHNTRDGASDSHDDITAIEKEDDSCAGGACKI
>Mature_762_residues
MNSNMTVTKRSGERETIDLDKIHRVIEWAAKGLNNVSVSEVELRSHLQFFDGIATEAIHETIIKAAADLISPESPDYQFL
SARLAVFHLRKKAFGQFEPPKLYDHVVKLIELGKYDQHILEDYSQEEIDTLDSYIDHWRDMNFSYAAVKQLEGKYLVQNR
VTHEIYESAQFLYILVAACLFAKYPKETRLQYVKDFYDATSTFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
SSSIVKYVSQRAGIGVNAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLK
NNRGVDDNRIRHLDYGVQINKVMYQRLIQGGMISLFSPSDVPGMYDAFFEDQEEFERLYLKYEADDSIRKKQIKAVELFS
LMMQERASTGRIYIQNVDHCNTHSPFDSKVAPVRQSNLCLEIALPTKPLNNINDPDGEIALCTLSALNLGAIKKLEELEP
LADLAVRALDNLLDYQDYPIISAQKASMNRRTLGIGVINFANYLAKEGVRYSDGSANGITHKTFEAIQFYLLKASMKLAK
EQGACPAFHETTYSKGILPIDTYKRDLDKICDEPLHLDWETLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRG
LISVKASKDGQLKQVVPDFEKYQYSYELLWQMPNNDGYLQLVGLMQKFVDQSISANTNYDPSRFEGGRVPMKVLLKDLLT
AYKYGVKTLYYHNTRDGASDSHDDITAIEKEDDSCAGGACKI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-cone domain [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=678, Percent_Identity=29.0560471976401, Blast_Score=229, Evalue=1e-59,
Organism=Escherichia coli, GI2367133, Length=762, Percent_Identity=80.9711286089239, Blast_Score=1316, Evalue=0.0,
Organism=Escherichia coli, GI1789030, Length=665, Percent_Identity=24.5112781954887, Blast_Score=163, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17555242, Length=692, Percent_Identity=27.8901734104046, Blast_Score=236, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6322123, Length=674, Percent_Identity=28.9317507418398, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6320914, Length=674, Percent_Identity=27.7448071216617, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI17352481, Length=672, Percent_Identity=29.6130952380952, Blast_Score=245, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 85826; Mature: 85826

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS51161 ATP_CONE ; PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSNMTVTKRSGERETIDLDKIHRVIEWAAKGLNNVSVSEVELRSHLQFFDGIATEAIHE
CCCCCEEEECCCCCCEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TIIKAAADLISPESPDYQFLSARLAVFHLRKKAFGQFEPPKLYDHVVKLIELGKYDQHIL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHH
EDYSQEEIDTLDSYIDHWRDMNFSYAAVKQLEGKYLVQNRVTHEIYESAQFLYILVAACL
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FAKYPKETRLQYVKDFYDATSTFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
HHHCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCHHHHCCEEEEEECCCCCCCCCC
SSSIVKYVSQRAGIGVNAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
AATLFYPMWHLEVESLLVLKNNRGVDDNRIRHLDYGVQINKVMYQRLIQGGMISLFSPSD
CHHHHHHHHHHEEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCC
VPGMYDAFFEDQEEFERLYLKYEADDSIRKKQIKAVELFSLMMQERASTGRIYIQNVDHC
CCCHHHHHHCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDSKVAPVRQSNLCLEIALPTKPLNNINDPDGEIALCTLSALNLGAIKKLEELEP
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEHHHCHHHHHHHHHHHH
LADLAVRALDNLLDYQDYPIISAQKASMNRRTLGIGVINFANYLAKEGVRYSDGSANGIT
HHHHHHHHHHHHHCCCCCCCEECHHHCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCC
HKTFEAIQFYLLKASMKLAKEQGACPAFHETTYSKGILPIDTYKRDLDKICDEPLHLDWE
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHH
TLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGLISVKASKDGQLKQVVPDFE
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCHH
KYQYSYELLWQMPNNDGYLQLVGLMQKFVDQSISANTNYDPSRFEGGRVPMKVLLKDLLT
HHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKYGVKTLYYHNTRDGASDSHDDITAIEKEDDSCAGGACKI
HHHHCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNSNMTVTKRSGERETIDLDKIHRVIEWAAKGLNNVSVSEVELRSHLQFFDGIATEAIHE
CCCCCEEEECCCCCCEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TIIKAAADLISPESPDYQFLSARLAVFHLRKKAFGQFEPPKLYDHVVKLIELGKYDQHIL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHH
EDYSQEEIDTLDSYIDHWRDMNFSYAAVKQLEGKYLVQNRVTHEIYESAQFLYILVAACL
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FAKYPKETRLQYVKDFYDATSTFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
HHHCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCHHHHCCEEEEEECCCCCCCCCC
SSSIVKYVSQRAGIGVNAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
AATLFYPMWHLEVESLLVLKNNRGVDDNRIRHLDYGVQINKVMYQRLIQGGMISLFSPSD
CHHHHHHHHHHEEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCC
VPGMYDAFFEDQEEFERLYLKYEADDSIRKKQIKAVELFSLMMQERASTGRIYIQNVDHC
CCCHHHHHHCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDSKVAPVRQSNLCLEIALPTKPLNNINDPDGEIALCTLSALNLGAIKKLEELEP
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEHHHCHHHHHHHHHHHH
LADLAVRALDNLLDYQDYPIISAQKASMNRRTLGIGVINFANYLAKEGVRYSDGSANGIT
HHHHHHHHHHHHHCCCCCCCEECHHHCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCC
HKTFEAIQFYLLKASMKLAKEQGACPAFHETTYSKGILPIDTYKRDLDKICDEPLHLDWE
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHH
TLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGLISVKASKDGQLKQVVPDFE
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCHH
KYQYSYELLWQMPNNDGYLQLVGLMQKFVDQSISANTNYDPSRFEGGRVPMKVLLKDLLT
HHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKYGVKTLYYHNTRDGASDSHDDITAIEKEDDSCAGGACKI
HHHHCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6087316; 3287341; 9205837; 9278503; 3894026; 2663852; 8702814; 8052308; 9309223; 10493864 [H]