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The map label for this gene is gph [C]

Identifier: 157961886

GI number: 157961886

Start: 2501398

End: 2502081

Strand: Reverse

Name: gph [C]

Synonym: Spea_2065

Alternate gene names: 157961886

Gene position: 2502081-2501398 (Counterclockwise)

Preceding gene: 157961887

Following gene: 157961885

Centisome position: 48.35

GC content: 48.1

Gene sequence:

>684_bases
ATGACTAAGGCAGAAGCATTACAGATTAAAGGCGTACTGTTCGATCTCGATGGTACGCTAGTCGATACCGCGCCAGATAT
GATTGAGGCTCTCAACATCAGTCTAGAAGCCCATGGGCATTCACGGGTGCCATTCGAGCAGATCCGCGACAGTGCGTCCC
ATGGCAGCATAGCCATGATTAGGGCGGCCCTGCCCCATATTGATGAGCAATACATTGCCCAATTACAGCAATCACTCTTC
GATAACTATGAGCGTATAAACGGTAACGATTGCTGTTTATTTGAAGGCTTAGACAGGTTACTCGAAACGCTTCACGGGCT
TTCTATTCCGTATGGTGTGGTAACCAATAAACCAGCTCGCTTCTCACGACCGCTACTCAATCGACTCGGCCTTACGGCAA
AAATGCCTACTGTGATAAGCGGAGACACAACGCTTTATTCCAAGCCTCATACGGCGCCTATGCATTTAGCCGCGCAGCAA
CTTAAGGTATTGCCTGAGCATATTCTGTATTTAGGTGATGCAGAGCGCGATCTTGTCGCGGCTAAAGCGGCAAATATGAT
CTCAGGTCTTGCCCTGTGGGGTTATATTGGCGCTAATGATGATATTTCGCTTTGGCCTGCCGATCTCAGCTTTGAGACTG
GCGGTCATTTAGCTGATTTTTTTACGGTTATGCAGCAGAGTTAA

Upstream 100 bases:

>100_bases
TTCTGCAAAGATGCTGTCGGGATCACCTATAACCCAATTACAGACATTTTTAGATATACTAAGAGTCTTGAAGTGAACTA
TATGATTGCCACGGTTAAAA

Downstream 100 bases:

>100_bases
TTTTCTAAGCGTACACATTTTAATCCATGATGATTTAGGCTTGTAAACTTCATACTAAAGTCGTCAAAATGATCTGCCTG
GATCGCTCAACTAAGTGGCG

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTKAEALQIKGVLFDLDGTLVDTAPDMIEALNISLEAHGHSRVPFEQIRDSASHGSIAMIRAALPHIDEQYIAQLQQSLF
DNYERINGNDCCLFEGLDRLLETLHGLSIPYGVVTNKPARFSRPLLNRLGLTAKMPTVISGDTTLYSKPHTAPMHLAAQQ
LKVLPEHILYLGDAERDLVAAKAANMISGLALWGYIGANDDISLWPADLSFETGGHLADFFTVMQQS

Sequences:

>Translated_227_residues
MTKAEALQIKGVLFDLDGTLVDTAPDMIEALNISLEAHGHSRVPFEQIRDSASHGSIAMIRAALPHIDEQYIAQLQQSLF
DNYERINGNDCCLFEGLDRLLETLHGLSIPYGVVTNKPARFSRPLLNRLGLTAKMPTVISGDTTLYSKPHTAPMHLAAQQ
LKVLPEHILYLGDAERDLVAAKAANMISGLALWGYIGANDDISLWPADLSFETGGHLADFFTVMQQS
>Mature_226_residues
TKAEALQIKGVLFDLDGTLVDTAPDMIEALNISLEAHGHSRVPFEQIRDSASHGSIAMIRAALPHIDEQYIAQLQQSLFD
NYERINGNDCCLFEGLDRLLETLHGLSIPYGVVTNKPARFSRPLLNRLGLTAKMPTVISGDTTLYSKPHTAPMHLAAQQL
KVLPEHILYLGDAERDLVAAKAANMISGLALWGYIGANDDISLWPADLSFETGGHLADFFTVMQQS

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=216, Percent_Identity=32.8703703703704, Blast_Score=100, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24882; Mature: 24751

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAEALQIKGVLFDLDGTLVDTAPDMIEALNISLEAHGHSRVPFEQIRDSASHGSIAMI
CCCCHHEEEEEEEEECCCCEECCCHHHHHHHHCEEEECCCCCCCHHHHHCCCCCCCHHHH
RAALPHIDEQYIAQLQQSLFDNYERINGNDCCLFEGLDRLLETLHGLSIPYGVVTNKPAR
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
FSRPLLNRLGLTAKMPTVISGDTTLYSKPHTAPMHLAAQQLKVLPEHILYLGDAERDLVA
HHHHHHHHHCCCCCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHHH
AKAANMISGLALWGYIGANDDISLWPADLSFETGGHLADFFTVMQQS
HHHHHHHHHHHHHHEECCCCCCEEECCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TKAEALQIKGVLFDLDGTLVDTAPDMIEALNISLEAHGHSRVPFEQIRDSASHGSIAMI
CCCHHEEEEEEEEECCCCEECCCHHHHHHHHCEEEECCCCCCCHHHHHCCCCCCCHHHH
RAALPHIDEQYIAQLQQSLFDNYERINGNDCCLFEGLDRLLETLHGLSIPYGVVTNKPAR
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
FSRPLLNRLGLTAKMPTVISGDTTLYSKPHTAPMHLAAQQLKVLPEHILYLGDAERDLVA
HHHHHHHHHCCCCCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHHH
AKAANMISGLALWGYIGANDDISLWPADLSFETGGHLADFFTVMQQS
HHHHHHHHHHHHHHEECCCCCCEEECCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA