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The map label for this gene is nrdB [H]

Identifier: 157961884

GI number: 157961884

Start: 2497455

End: 2498585

Strand: Reverse

Name: nrdB [H]

Synonym: Spea_2063

Alternate gene names: 157961884

Gene position: 2498585-2497455 (Counterclockwise)

Preceding gene: 157961885

Following gene: 157961883

Centisome position: 48.29

GC content: 41.2

Gene sequence:

>1131_bases
ATGGCCTATTCAACATTTTGTCAAACTCCAAACAACGCAATGCTAGAGCCTATGTTTCTTGGGCAATCAGTAAACGTAGC
GCGTTATGACCAACAAAAATATGAAGTATTCGAAAAGCTAATCGAAAAGCAGCTTTCTTTCTTCTGGCGTCCTGAAGAAG
TTGACGTTAGCCGAGATAAAATCGATTACGCTGCATTGCCTGAGCATGAAAAGCATATTTTCATTTCGAACCTGAAATAT
CAAACTCTGCTTGATTCAATTCAAGGCCGTTCACCAAACGTTGCCTTCTTGCCACTGGTATCACTACCAGAGTTAGAAAC
ATGGATTGAGACCTGGTCATTCTCTGAGACGATTCACTCACGTTCGTACACTCATATTATTCGTAATATTGTTAACGATC
CTTCAATCGTATTTAACGACATCGTAGAAAATGAAGAGATCTTAAAGCGCGCTAGCGATATCGCTAACTATTACGATCAC
TTGATTAAGCTAAGCCAGGCTTATCACCTATTAGGTGAAGGTAGCCACGAGATCGACGGCGAAATCATTGAAGTCACTAA
GCGTGAAATCAAGAAAGCGCTGTACCTATGTATGGTATCGGTTAACGTACTCGAAGCTATCCGCTTCTACGTCAGTTTTG
CTTGTTCATTTGCCTTTGCCGAGCGCAAGGTTATGGAAGGTAATGCAAAGATTATTCGCTTGATCGCTCGCGATGAAGCA
TTGCATCTAAACAGCACTCAGCATATTTTAAAGATTATGCATGCTGGTAAAGACGACCCTGAAATGGCCGAAATTGCTAA
AGAGTGCGAGCAAACTGCAATTGATATTTTTATCAAAGCAGCCGAGCAGGAAAAAGAGTGGGCTAAATACCTATTTAAAG
ATGGTTCTATGATTGGTCTAAACGAAACCATTCTGTGCCAATACGTTGAATACATCACTAACGAGCGCATGAAAGCGGTG
AACTTTGCTTCTCCTTATGCAGAGCAAACTAACCCACTACCATGGATGAAGAACTGGTTAGAGAGTGATTCAGTGCAAGT
TGCACCGCAAGAAGTAGAAGTCTCTTCTTATCTTGTTGGTCAAATCGATTCTTCAATCGATGACAATGAGTTCTCAGACT
TTGACCTCTAA

Upstream 100 bases:

>100_bases
TTGCGCTGGCGGCGCATGTAAGATCTAACCCAAGATATTAATCATTAGTATTAAACGTTAATTTAAGGGGGCCTAGCCCC
CATTATCGGAAGATAGAAAA

Downstream 100 bases:

>100_bases
GCCTGTCAGCTTTAGTCAAGCAAGCTTTAAGAAGGCTCCGATTGTGAGTCTTCAAGGCATGCCGGTGTTGCTGTTTAATC
AGCAGCACCAGACTTTGCTT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAYSTFCQTPNNAMLEPMFLGQSVNVARYDQQKYEVFEKLIEKQLSFFWRPEEVDVSRDKIDYAALPEHEKHIFISNLKY
QTLLDSIQGRSPNVAFLPLVSLPELETWIETWSFSETIHSRSYTHIIRNIVNDPSIVFNDIVENEEILKRASDIANYYDH
LIKLSQAYHLLGEGSHEIDGEIIEVTKREIKKALYLCMVSVNVLEAIRFYVSFACSFAFAERKVMEGNAKIIRLIARDEA
LHLNSTQHILKIMHAGKDDPEMAEIAKECEQTAIDIFIKAAEQEKEWAKYLFKDGSMIGLNETILCQYVEYITNERMKAV
NFASPYAEQTNPLPWMKNWLESDSVQVAPQEVEVSSYLVGQIDSSIDDNEFSDFDL

Sequences:

>Translated_376_residues
MAYSTFCQTPNNAMLEPMFLGQSVNVARYDQQKYEVFEKLIEKQLSFFWRPEEVDVSRDKIDYAALPEHEKHIFISNLKY
QTLLDSIQGRSPNVAFLPLVSLPELETWIETWSFSETIHSRSYTHIIRNIVNDPSIVFNDIVENEEILKRASDIANYYDH
LIKLSQAYHLLGEGSHEIDGEIIEVTKREIKKALYLCMVSVNVLEAIRFYVSFACSFAFAERKVMEGNAKIIRLIARDEA
LHLNSTQHILKIMHAGKDDPEMAEIAKECEQTAIDIFIKAAEQEKEWAKYLFKDGSMIGLNETILCQYVEYITNERMKAV
NFASPYAEQTNPLPWMKNWLESDSVQVAPQEVEVSSYLVGQIDSSIDDNEFSDFDL
>Mature_375_residues
AYSTFCQTPNNAMLEPMFLGQSVNVARYDQQKYEVFEKLIEKQLSFFWRPEEVDVSRDKIDYAALPEHEKHIFISNLKYQ
TLLDSIQGRSPNVAFLPLVSLPELETWIETWSFSETIHSRSYTHIIRNIVNDPSIVFNDIVENEEILKRASDIANYYDHL
IKLSQAYHLLGEGSHEIDGEIIEVTKREIKKALYLCMVSVNVLEAIRFYVSFACSFAFAERKVMEGNAKIIRLIARDEAL
HLNSTQHILKIMHAGKDDPEMAEIAKECEQTAIDIFIKAAEQEKEWAKYLFKDGSMIGLNETILCQYVEYITNERMKAVN
FASPYAEQTNPLPWMKNWLESDSVQVAPQEVEVSSYLVGQIDSSIDDNEFSDFDL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1788567, Length=376, Percent_Identity=73.6702127659574, Blast_Score=609, Evalue=1e-176,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 43414; Mature: 43282

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSTFCQTPNNAMLEPMFLGQSVNVARYDQQKYEVFEKLIEKQLSFFWRPEEVDVSRDK
CCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHC
IDYAALPEHEKHIFISNLKYQTLLDSIQGRSPNVAFLPLVSLPELETWIETWSFSETIHS
CCHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
RSYTHIIRNIVNDPSIVFNDIVENEEILKRASDIANYYDHLIKLSQAYHLLGEGSHEIDG
HHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EIIEVTKREIKKALYLCMVSVNVLEAIRFYVSFACSFAFAERKVMEGNAKIIRLIARDEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCC
LHLNSTQHILKIMHAGKDDPEMAEIAKECEQTAIDIFIKAAEQEKEWAKYLFKDGSMIGL
EECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
NETILCQYVEYITNERMKAVNFASPYAEQTNPLPWMKNWLESDSVQVAPQEVEVSSYLVG
CHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEECHHHHHHHHHHHH
QIDSSIDDNEFSDFDL
HHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AYSTFCQTPNNAMLEPMFLGQSVNVARYDQQKYEVFEKLIEKQLSFFWRPEEVDVSRDK
CCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHC
IDYAALPEHEKHIFISNLKYQTLLDSIQGRSPNVAFLPLVSLPELETWIETWSFSETIHS
CCHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
RSYTHIIRNIVNDPSIVFNDIVENEEILKRASDIANYYDHLIKLSQAYHLLGEGSHEIDG
HHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EIIEVTKREIKKALYLCMVSVNVLEAIRFYVSFACSFAFAERKVMEGNAKIIRLIARDEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCC
LHLNSTQHILKIMHAGKDDPEMAEIAKECEQTAIDIFIKAAEQEKEWAKYLFKDGSMIGL
EECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
NETILCQYVEYITNERMKAVNFASPYAEQTNPLPWMKNWLESDSVQVAPQEVEVSSYLVG
CHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEECHHHHHHHHHHHH
QIDSSIDDNEFSDFDL
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]