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The map label for this gene is hypB [H]

Identifier: 157961848

GI number: 157961848

Start: 2451691

End: 2452452

Strand: Reverse

Name: hypB [H]

Synonym: Spea_2027

Alternate gene names: 157961848

Gene position: 2452452-2451691 (Counterclockwise)

Preceding gene: 157961849

Following gene: 157961847

Centisome position: 47.39

GC content: 42.39

Gene sequence:

>762_bases
ATGTGCCAAGATTGTGGTTGTTCGATTACTCGTCATGATCATTTATTAAGTTCGGGTCATTCGCATTCAAAAACTGACGA
TAATATCGCAACGAACCCTCAGCTCAACGATAAAAAGACCCTATCTCTCATTCATAAGATTTTAGATAAAAACGATATTG
AAGCGCAGCATAACCGAGCTCATTTCGAAGCTAAAGGGATCACCGCCTTTAATTTAATGAGCAGTCCTGGTAGTGGTAAA
ACCACACTGTTAGAACATCTACATGAATACACTAACCTTAAGTATGCTGTTATTGAAGGGGATCTTGAAACCTCCAGAGA
TGCAGACAGACTAAAAGCAAAAGGTATCGATGCCTTTCAGATCCAAACTGGTGCAGCTTGTCATCTAGATGCGTTTATGG
TGCATAGCGCCTTACATCACATTAACTTAGACCCTCTGGATATCTGCTTTGTTGAAAATGTCGGCAACTTAGTCTGCCCC
GCCAGTTACGATGTTGGCACTCATAAAAATATCGTGCTTTTGTCTGTGCCTGAAGGCGATGACAAAATTGAGAAGTACCC
CGTCATGTTCCGCCGTGCAGATTTAGTGCTGATCACTAAGTCTGATCTTATGCCCTATTTTGACTTTAGCATGGAGGAGT
CACGGGCCCAGCTCAAAAAACTGAATCCCAACGTTGAGTTAATGGAGATATCTGTTAAAGAGCCACAATCACTCATTCAG
GTCGCAGACTGGCTTTCAAGACAAAAGGGAGCAACTGCCTAA

Upstream 100 bases:

>100_bases
ATATCCATCACCACTCAATAGACATAAATCTGATTTGCATCAAAGCCGTAGTATTCGGCCTGCGTGACAATCAAGTAAAA
CGAAAATAACGGAGTTACCT

Downstream 100 bases:

>100_bases
TGTGTCTTTCAATTCCCTCCAAAGTTATAACTATTCATCAAGATGAGCAGGCTGTTACCGTCGATACTATGGGCGTTAAA
CGTAAGGTCAGTAGCCACCT

Product: hydrogenase accessory protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MCQDCGCSITRHDHLLSSGHSHSKTDDNIATNPQLNDKKTLSLIHKILDKNDIEAQHNRAHFEAKGITAFNLMSSPGSGK
TTLLEHLHEYTNLKYAVIEGDLETSRDADRLKAKGIDAFQIQTGAACHLDAFMVHSALHHINLDPLDICFVENVGNLVCP
ASYDVGTHKNIVLLSVPEGDDKIEKYPVMFRRADLVLITKSDLMPYFDFSMEESRAQLKKLNPNVELMEISVKEPQSLIQ
VADWLSRQKGATA

Sequences:

>Translated_253_residues
MCQDCGCSITRHDHLLSSGHSHSKTDDNIATNPQLNDKKTLSLIHKILDKNDIEAQHNRAHFEAKGITAFNLMSSPGSGK
TTLLEHLHEYTNLKYAVIEGDLETSRDADRLKAKGIDAFQIQTGAACHLDAFMVHSALHHINLDPLDICFVENVGNLVCP
ASYDVGTHKNIVLLSVPEGDDKIEKYPVMFRRADLVLITKSDLMPYFDFSMEESRAQLKKLNPNVELMEISVKEPQSLIQ
VADWLSRQKGATA
>Mature_253_residues
MCQDCGCSITRHDHLLSSGHSHSKTDDNIATNPQLNDKKTLSLIHKILDKNDIEAQHNRAHFEAKGITAFNLMSSPGSGK
TTLLEHLHEYTNLKYAVIEGDLETSRDADRLKAKGIDAFQIQTGAACHLDAFMVHSALHHINLDPLDICFVENVGNLVCP
ASYDVGTHKNIVLLSVPEGDDKIEKYPVMFRRADLVLITKSDLMPYFDFSMEESRAQLKKLNPNVELMEISVKEPQSLIQ
VADWLSRQKGATA

Specific function: Plays a role in both the hydrogenase and the urease nickel cofactor insertion step [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=221, Percent_Identity=44.3438914027149, Blast_Score=191, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 28218; Mature: 28218

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQDCGCSITRHDHLLSSGHSHSKTDDNIATNPQLNDKKTLSLIHKILDKNDIEAQHNRA
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHCCC
HFEAKGITAFNLMSSPGSGKTTLLEHLHEYTNLKYAVIEGDLETSRDADRLKAKGIDAFQ
EEEECCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHCCCCEEE
IQTGAACHLDAFMVHSALHHINLDPLDICFVENVGNLVCPASYDVGTHKNIVLLSVPEGD
ECCCCCHHHHHHHHHHHHHHCCCCHHEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCC
DKIEKYPVMFRRADLVLITKSDLMPYFDFSMEESRAQLKKLNPNVELMEISVKEPQSLIQ
CHHHHCCCEEEECCEEEEECCCCCCHHCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHH
VADWLSRQKGATA
HHHHHHHCCCCCC
>Mature Secondary Structure
MCQDCGCSITRHDHLLSSGHSHSKTDDNIATNPQLNDKKTLSLIHKILDKNDIEAQHNRA
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHCCC
HFEAKGITAFNLMSSPGSGKTTLLEHLHEYTNLKYAVIEGDLETSRDADRLKAKGIDAFQ
EEEECCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHCCCCEEE
IQTGAACHLDAFMVHSALHHINLDPLDICFVENVGNLVCPASYDVGTHKNIVLLSVPEGD
ECCCCCHHHHHHHHHHHHHHCCCCHHEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCC
DKIEKYPVMFRRADLVLITKSDLMPYFDFSMEESRAQLKKLNPNVELMEISVKEPQSLIQ
CHHHHCCCEEEECCEEEEECCCCCCHHCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHH
VADWLSRQKGATA
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954 [H]