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The map label for this gene is kdsB [H]

Identifier: 157961808

GI number: 157961808

Start: 2411113

End: 2411850

Strand: Reverse

Name: kdsB [H]

Synonym: Spea_1985

Alternate gene names: 157961808

Gene position: 2411850-2411113 (Counterclockwise)

Preceding gene: 157961809

Following gene: 157961807

Centisome position: 46.61

GC content: 43.36

Gene sequence:

>738_bases
ATGAATGTAACCTTATTAATCCCTGCGCGATATGGATCGAGTCGTTTTCCAGGTAAGCCATTGGCTCATATCAATGGTAA
GCCAATGATCCAGCACGTTTACGAGCGTGCATCTTTAGCTAAAGGGCTTAAAGACATCTATGTCGCCACCGATGATGTGC
GCATTAAAAACGCTGTAGAGAGTTTTGGTGGCAAGGTTGTTATGACAGGTGAAAATGCCGCATCTGGTACAGACCGTATC
GATGAAGCTATTTCAATACTCGGCTTGGCTGATGATGACCTCGTCATTAATTTACAAGGTGATCAACCGCTTATCGACCC
AATTGTTATTGAACAACTTGTAAGCGTGTGTGAACGCCATGCTGGTGAATTCGATATGGCGACGTTAGGCGTAGAGATAA
AGGATGAAGCTCAGCTTAACGATCCAAACCATGTAAAAGTGGTATTCGATAATAACCATAACGCATTATACTTTTCTCGA
GCGCGGATCCCTTTTGGCCGTGATACGAGCGACTACCCGGTATACAAGCACATAGGGATCTACGCCTATACTCGTAAATT
TATTCAAACCTTTGCTAAGTTACCACTTGGTCGACTAGAAGAGTTAGAGAAGTTGGAACAACTTCGAGCGCTTGAGTACG
GCTACAAAATCAAAGTTGCAATCAGCGCATTTGATTTCCCAGAAGTTGATACTCCTGAAGATATTCGAATCTGCGAAGCA
AGACTTACCGTTGACTAA

Upstream 100 bases:

>100_bases
AAATGCTCACTCAGTTATTCCGTCGGTTCTAAACTCAGCATAGGCATAAAAATGGGCTTCATCTTAAGGCCCATGATATT
TTCTTAAGTAGGTAAATTGA

Downstream 100 bases:

>100_bases
TTGAGGATGAATGATGTCAGATCTTGAAGTCGGTTTATTGATTTTATTAGTGATCGGAGTGATTGCCAGTAATCTTGCGG
TATTAAAGTACAGTGCAAAA

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MNVTLLIPARYGSSRFPGKPLAHINGKPMIQHVYERASLAKGLKDIYVATDDVRIKNAVESFGGKVVMTGENAASGTDRI
DEAISILGLADDDLVINLQGDQPLIDPIVIEQLVSVCERHAGEFDMATLGVEIKDEAQLNDPNHVKVVFDNNHNALYFSR
ARIPFGRDTSDYPVYKHIGIYAYTRKFIQTFAKLPLGRLEELEKLEQLRALEYGYKIKVAISAFDFPEVDTPEDIRICEA
RLTVD

Sequences:

>Translated_245_residues
MNVTLLIPARYGSSRFPGKPLAHINGKPMIQHVYERASLAKGLKDIYVATDDVRIKNAVESFGGKVVMTGENAASGTDRI
DEAISILGLADDDLVINLQGDQPLIDPIVIEQLVSVCERHAGEFDMATLGVEIKDEAQLNDPNHVKVVFDNNHNALYFSR
ARIPFGRDTSDYPVYKHIGIYAYTRKFIQTFAKLPLGRLEELEKLEQLRALEYGYKIKVAISAFDFPEVDTPEDIRICEA
RLTVD
>Mature_245_residues
MNVTLLIPARYGSSRFPGKPLAHINGKPMIQHVYERASLAKGLKDIYVATDDVRIKNAVESFGGKVVMTGENAASGTDRI
DEAISILGLADDDLVINLQGDQPLIDPIVIEQLVSVCERHAGEFDMATLGVEIKDEAQLNDPNHVKVVFDNNHNALYFSR
ARIPFGRDTSDYPVYKHIGIYAYTRKFIQTFAKLPLGRLEELEKLEQLRALEYGYKIKVAISAFDFPEVDTPEDIRICEA
RLTVD

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria [H]

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family [H]

Homologues:

Organism=Escherichia coli, GI1787147, Length=252, Percent_Identity=44.4444444444444, Blast_Score=190, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003329
- InterPro:   IPR004528 [H]

Pfam domain/function: PF02348 CTP_transf_3 [H]

EC number: =2.7.7.38 [H]

Molecular weight: Translated: 27394; Mature: 27394

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVTLLIPARYGSSRFPGKPLAHINGKPMIQHVYERASLAKGLKDIYVATDDVRIKNAVE
CCEEEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHH
SFGGKVVMTGENAASGTDRIDEAISILGLADDDLVINLQGDQPLIDPIVIEQLVSVCERH
HHCCEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHC
AGEFDMATLGVEIKDEAQLNDPNHVKVVFDNNHNALYFSRARIPFGRDTSDYPVYKHIGI
CCCCCEEEECEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHH
YAYTRKFIQTFAKLPLGRLEELEKLEQLRALEYGYKIKVAISAFDFPEVDTPEDIRICEA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCEEEEE
RLTVD
EEECC
>Mature Secondary Structure
MNVTLLIPARYGSSRFPGKPLAHINGKPMIQHVYERASLAKGLKDIYVATDDVRIKNAVE
CCEEEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHH
SFGGKVVMTGENAASGTDRIDEAISILGLADDDLVINLQGDQPLIDPIVIEQLVSVCERH
HHCCEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHC
AGEFDMATLGVEIKDEAQLNDPNHVKVVFDNNHNALYFSRARIPFGRDTSDYPVYKHIGI
CCCCCEEEECEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHH
YAYTRKFIQTFAKLPLGRLEELEKLEQLRALEYGYKIKVAISAFDFPEVDTPEDIRICEA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCEEEEE
RLTVD
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA