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The map label for this gene is iciA [H]

Identifier: 157961797

GI number: 157961797

Start: 2400010

End: 2400900

Strand: Reverse

Name: iciA [H]

Synonym: Spea_1974

Alternate gene names: 157961797

Gene position: 2400900-2400010 (Counterclockwise)

Preceding gene: 157961801

Following gene: 157961794

Centisome position: 46.4

GC content: 44.0

Gene sequence:

>891_bases
ATGTTTGACTACGCCCACCTCAAAGCACTATCCATTGTCGTGTCTCAAGGAGGATTTGATAAGGCAGCTCAACATCTGTA
TATCAGTCAATCTGCGGTTTCTCAAAGGATCAAGCAACTAGAAGATAAGGTTGGTCAGGCGCTATTGGTGAGATCAAACC
CTGTCAGAGCCACTGAAACGGGTAAACGGCTTCTCAGACATTATGCACAAGTGCAATTACTTGAAAGCGAACTTCGCTCT
GAAATGGACGTAGATGATCCCTCCCTTCCTACCATTGTTAAGATTGCAGTCAATGCGGATAGCCTAGCTACCTGGTTTCT
ACCAGCATTAACTCAGACATTTAAGCAGCATAAGTGGCTGCTAGAACTGGTTGTCGATGATGAGGCTTATACCCACCATC
TACTCAAAAATGGCGAAGCTGTTGGCTGTGTTACGACCACACAACTGGCGCTGTCGGGCTGTAGCAGTGAGTTCCTCGGT
AATATTGAATATCAATGTGTAGCGACGCCTAGCTTTATTGAGCAATATTTTAGCTCTGACGTGCCCTTAGATGACATTTC
TCATGCGCCAGCAGTGGTGTTCTCAACTAAAGACAAGCTGCATGATAAGTTCCTTAGTTTCTATTTTGACATTAATGACA
AGAGTTGGCGTGAACATAAAATCCCCTCTTCCGAAAGTTTTCTTGAAGCCATTAAGATGGGATTAGGCTATGGGTTAGTC
GCACATTTGCAGGCCAAGCCATTGTTAGATTCAGGCGAATTAGTAGAAATCTTTCCTCACAAAAGAATGCAAGTACCATT
GTATTGGCAACACTGGAACATTAAGGCCAAACAAACCAGCATTATTTACCGAGCTTTAGCTATGAGCGCCAAGGAGCAGT
TATTCCCCTAG

Upstream 100 bases:

>100_bases
TGGCGCAAGCATAGTCGGCAGCCAAAAATAAGTACAACTAATAAATCTAATAATGTATAAGTAAAGCTTATCTCACATAT
TTTTATAACTAGGAGCAAAG

Downstream 100 bases:

>100_bases
GTCGCTCTGGGGTAAGGATTAAAATTCTGAAGAAGGTGGCTTTGCCCATGTGTTACAAAATAGACTCCCTAAAAGGGAGC
TTTCCTACCGTGCGTTAACA

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MFDYAHLKALSIVVSQGGFDKAAQHLYISQSAVSQRIKQLEDKVGQALLVRSNPVRATETGKRLLRHYAQVQLLESELRS
EMDVDDPSLPTIVKIAVNADSLATWFLPALTQTFKQHKWLLELVVDDEAYTHHLLKNGEAVGCVTTTQLALSGCSSEFLG
NIEYQCVATPSFIEQYFSSDVPLDDISHAPAVVFSTKDKLHDKFLSFYFDINDKSWREHKIPSSESFLEAIKMGLGYGLV
AHLQAKPLLDSGELVEIFPHKRMQVPLYWQHWNIKAKQTSIIYRALAMSAKEQLFP

Sequences:

>Translated_296_residues
MFDYAHLKALSIVVSQGGFDKAAQHLYISQSAVSQRIKQLEDKVGQALLVRSNPVRATETGKRLLRHYAQVQLLESELRS
EMDVDDPSLPTIVKIAVNADSLATWFLPALTQTFKQHKWLLELVVDDEAYTHHLLKNGEAVGCVTTTQLALSGCSSEFLG
NIEYQCVATPSFIEQYFSSDVPLDDISHAPAVVFSTKDKLHDKFLSFYFDINDKSWREHKIPSSESFLEAIKMGLGYGLV
AHLQAKPLLDSGELVEIFPHKRMQVPLYWQHWNIKAKQTSIIYRALAMSAKEQLFP
>Mature_296_residues
MFDYAHLKALSIVVSQGGFDKAAQHLYISQSAVSQRIKQLEDKVGQALLVRSNPVRATETGKRLLRHYAQVQLLESELRS
EMDVDDPSLPTIVKIAVNADSLATWFLPALTQTFKQHKWLLELVVDDEAYTHHLLKNGEAVGCVTTTQLALSGCSSEFLG
NIEYQCVATPSFIEQYFSSDVPLDDISHAPAVVFSTKDKLHDKFLSFYFDINDKSWREHKIPSSESFLEAIKMGLGYGLV
AHLQAKPLLDSGELVEIFPHKRMQVPLYWQHWNIKAKQTSIIYRALAMSAKEQLFP

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=287, Percent_Identity=41.1149825783972, Blast_Score=199, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33423; Mature: 33423

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDYAHLKALSIVVSQGGFDKAAQHLYISQSAVSQRIKQLEDKVGQALLVRSNPVRATET
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHH
GKRLLRHYAQVQLLESELRSEMDVDDPSLPTIVKIAVNADSLATWFLPALTQTFKQHKWL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
LELVVDDEAYTHHLLKNGEAVGCVTTTQLALSGCSSEFLGNIEYQCVATPSFIEQYFSSD
HHHEECCHHHHHHHHHCCCEEEEEHHHHHHHHCCCHHHHCCCEEEEEECHHHHHHHHCCC
VPLDDISHAPAVVFSTKDKLHDKFLSFYFDINDKSWREHKIPSSESFLEAIKMGLGYGLV
CCCCCCCCCCEEEEECHHHHHHHHHHHEEECCCCCCHHCCCCCHHHHHHHHHHHCCCHHH
AHLQAKPLLDSGELVEIFPHKRMQVPLYWQHWNIKAKQTSIIYRALAMSAKEQLFP
HHHCCCCCCCCCCEEEECCCCCEECEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFDYAHLKALSIVVSQGGFDKAAQHLYISQSAVSQRIKQLEDKVGQALLVRSNPVRATET
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHH
GKRLLRHYAQVQLLESELRSEMDVDDPSLPTIVKIAVNADSLATWFLPALTQTFKQHKWL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
LELVVDDEAYTHHLLKNGEAVGCVTTTQLALSGCSSEFLGNIEYQCVATPSFIEQYFSSD
HHHEECCHHHHHHHHHCCCEEEEEHHHHHHHHCCCHHHHCCCEEEEEECHHHHHHHHCCC
VPLDDISHAPAVVFSTKDKLHDKFLSFYFDINDKSWREHKIPSSESFLEAIKMGLGYGLV
CCCCCCCCCCEEEEECHHHHHHHHHHHEEECCCCCCHHCCCCCHHHHHHHHHHHCCCHHH
AHLQAKPLLDSGELVEIFPHKRMQVPLYWQHWNIKAKQTSIIYRALAMSAKEQLFP
HHHCCCCCCCCCCEEEECCCCCEECEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA