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The map label for this gene is holB [H]

Identifier: 157961747

GI number: 157961747

Start: 2334666

End: 2335583

Strand: Direct

Name: holB [H]

Synonym: Spea_1924

Alternate gene names: 157961747

Gene position: 2334666-2335583 (Clockwise)

Preceding gene: 157961746

Following gene: 157961748

Centisome position: 45.12

GC content: 45.1

Gene sequence:

>918_bases
ATGACGGCGCTTCCTTGGCTGCAACCCACACTGGATAAATTCTCTGAACAGCTCAAGACCGCTAGAGTTGGTCATGCCTA
TCTTGTTGGTATGGATGCGGGCTATGGCGGAGAGTCATTAGTACTCGAGTTAGCCAAAATGTCGCTCTGTCATCAGCTTG
GACAAGGTGGCGCTTGTGGATTTTGTAAGGCTTGCCAACTGATAGAAGCCAACAATCACCCGGATCTCTACCTGGTGGAG
GCCGAAGGTGCACAGATTAAGGTCGACCAGATCCGTGAGCTTTGTCAAAAGCTGACGACAACATCTCAACAAGGTGGTAG
ACGAGTCGCGGTCATTTTAAATAGTGAACGCTTAAATCAGGCATCAGGGAATGCCTTACTTAAGACACTCGAAGAGCCTG
GAAAGGATACAATTTTGCTCTTACAGACGAATGCACCTAGTCGATTAATCGCGACTATTAGTAGTCGTTGCCAAAGGTTG
CGTGTTGAACTGCCAAACAAAATGCAAGTTAAGCAGTGGCTTAAAGAGCAGTTGACCACGCAAGATGACCCATCTTGGTG
TTTACCCGTTGTTGGCGGGCCGATGGCTGTGTTATCTGCCATTGAAACTGAGCGATATAAAACCCTGATAGATTACAGAA
AAGGCTGGACACAAAGTTTGTCGTCGGGTCATCTGTGTGCTAGTTTAATAAATGTTAATGAAAAGCAAGTTTCTGATGCT
CTTAAGGTTTTATACTTAGTATTGCATCGAGCTTTAGTTAAACAACAAGATAGCGATCCCCTCACTCGGGTCAAGATTGC
TAATTTTTCCGCGAAAGTGATGCAGTTAGAAACGCGTTTATCTGTGATGCCAACGGTCAATACGTTAGCGTTATTTGGCG
GGCTAACCATTGAGTATAATCAGCTAATCAACCAATAG

Upstream 100 bases:

>100_bases
GCCGATGCCGATGAGTCTATTAAAATCGTCGATGCCAGCCAGAGTATGGCTGAAGTTCATAAAGATATTATTGCTCTTCT
TCAAGAGCAGAGTTGGTAAT

Downstream 100 bases:

>100_bases
GAAACTATGACTAATCTTGTTGTCAAATTCGAATCTTTGCAGCAACTCTATCGAGCTTATATGCCGTTTATTAAGCCTAT
CGGTATGTTTGTAAGCTGTA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTALPWLQPTLDKFSEQLKTARVGHAYLVGMDAGYGGESLVLELAKMSLCHQLGQGGACGFCKACQLIEANNHPDLYLVE
AEGAQIKVDQIRELCQKLTTTSQQGGRRVAVILNSERLNQASGNALLKTLEEPGKDTILLLQTNAPSRLIATISSRCQRL
RVELPNKMQVKQWLKEQLTTQDDPSWCLPVVGGPMAVLSAIETERYKTLIDYRKGWTQSLSSGHLCASLINVNEKQVSDA
LKVLYLVLHRALVKQQDSDPLTRVKIANFSAKVMQLETRLSVMPTVNTLALFGGLTIEYNQLINQ

Sequences:

>Translated_305_residues
MTALPWLQPTLDKFSEQLKTARVGHAYLVGMDAGYGGESLVLELAKMSLCHQLGQGGACGFCKACQLIEANNHPDLYLVE
AEGAQIKVDQIRELCQKLTTTSQQGGRRVAVILNSERLNQASGNALLKTLEEPGKDTILLLQTNAPSRLIATISSRCQRL
RVELPNKMQVKQWLKEQLTTQDDPSWCLPVVGGPMAVLSAIETERYKTLIDYRKGWTQSLSSGHLCASLINVNEKQVSDA
LKVLYLVLHRALVKQQDSDPLTRVKIANFSAKVMQLETRLSVMPTVNTLALFGGLTIEYNQLINQ
>Mature_304_residues
TALPWLQPTLDKFSEQLKTARVGHAYLVGMDAGYGGESLVLELAKMSLCHQLGQGGACGFCKACQLIEANNHPDLYLVEA
EGAQIKVDQIRELCQKLTTTSQQGGRRVAVILNSERLNQASGNALLKTLEEPGKDTILLLQTNAPSRLIATISSRCQRLR
VELPNKMQVKQWLKEQLTTQDDPSWCLPVVGGPMAVLSAIETERYKTLIDYRKGWTQSLSSGHLCASLINVNEKQVSDAL
KVLYLVLHRALVKQQDSDPLTRVKIANFSAKVMQLETRLSVMPTVNTLALFGGLTIEYNQLINQ

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=277, Percent_Identity=30.3249097472924, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1786676, Length=171, Percent_Identity=32.1637426900585, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 33716; Mature: 33584

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALPWLQPTLDKFSEQLKTARVGHAYLVGMDAGYGGESLVLELAKMSLCHQLGQGGACG
CCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
FCKACQLIEANNHPDLYLVEAEGAQIKVDQIRELCQKLTTTSQQGGRRVAVILNSERLNQ
HHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
ASGNALLKTLEEPGKDTILLLQTNAPSRLIATISSRCQRLRVELPNKMQVKQWLKEQLTT
CCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
QDDPSWCLPVVGGPMAVLSAIETERYKTLIDYRKGWTQSLSSGHLCASLINVNEKQVSDA
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHH
LKVLYLVLHRALVKQQDSDPLTRVKIANFSAKVMQLETRLSVMPTVNTLALFGGLTIEYN
HHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHCCCEEEHH
QLINQ
HHHCC
>Mature Secondary Structure 
TALPWLQPTLDKFSEQLKTARVGHAYLVGMDAGYGGESLVLELAKMSLCHQLGQGGACG
CCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
FCKACQLIEANNHPDLYLVEAEGAQIKVDQIRELCQKLTTTSQQGGRRVAVILNSERLNQ
HHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
ASGNALLKTLEEPGKDTILLLQTNAPSRLIATISSRCQRLRVELPNKMQVKQWLKEQLTT
CCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
QDDPSWCLPVVGGPMAVLSAIETERYKTLIDYRKGWTQSLSSGHLCASLINVNEKQVSDA
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHH
LKVLYLVLHRALVKQQDSDPLTRVKIANFSAKVMQLETRLSVMPTVNTLALFGGLTIEYN
HHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHCCCEEEHH
QLINQ
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]