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The map label for this gene is tmk [H]

Identifier: 157961746

GI number: 157961746

Start: 2334020

End: 2334664

Strand: Direct

Name: tmk [H]

Synonym: Spea_1923

Alternate gene names: 157961746

Gene position: 2334020-2334664 (Clockwise)

Preceding gene: 157961745

Following gene: 157961747

Centisome position: 45.11

GC content: 48.06

Gene sequence:

>645_bases
ATGAATACACCAGTAACAGCAGCGCAATCAGCCAAATTTATCGTTATCGAAGGGCTAGAAGGGGCAGGCAAGTCAAGCGC
GATAGCCCTTGTTCGAGACTTTATTGAAAAGCATACTGGTAAAGCACCAATTTGCACCCGTGAGCCAGGTGGTACACCGC
TCGCCGAGCAGATGCGCGATTTGGTGAAAATTGCTAACGAATCAGACCCTCTTTGCGATGAAGCCGAATGCCTACTCTTT
TATGCGGCGAGAGCGCAACTGGTTGCTAACGTGATTAAACCAGCTTTAGCGAACGGCGAGTGGGTGTTAGGCGATAGACA
TAACTTGTCTTCTCTCGCCTATCAAGGTGGGGGGCGTAAACTGATGCCTTTGGTATCGGCGATTAGCGATGCCACGTTAA
AAGGTTTCAAGCCTGATTTAACACTCTATCTCGATATAGACCCTAAGCTGGGTCTCGAGCGTGCGGCTCGCCGGGGTGAG
CTGGATCGTATTGAGCAACAAGAAATCGAATTTTTTAAACGTACTAGGGCGACTTTCTTGTCGTTCGCCGATGCCGATGA
GTCTATTAAAATCGTCGATGCCAGCCAGAGTATGGCTGAAGTTCATAAAGATATTATTGCTCTTCTTCAAGAGCAGAGTT
GGTAA

Upstream 100 bases:

>100_bases
TAAATTATATTTATTTCGTCTCACGAAATGATGGTAGCCATGTCTTTTCAAGAACACTGAACGAGCATAATCGTGCAGTG
AACAAATATCAGAGAAATCG

Downstream 100 bases:

>100_bases
TATGACGGCGCTTCCTTGGCTGCAACCCACACTGGATAAATTCTCTGAACAGCTCAAGACCGCTAGAGTTGGTCATGCCT
ATCTTGTTGGTATGGATGCG

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNTPVTAAQSAKFIVIEGLEGAGKSSAIALVRDFIEKHTGKAPICTREPGGTPLAEQMRDLVKIANESDPLCDEAECLLF
YAARAQLVANVIKPALANGEWVLGDRHNLSSLAYQGGGRKLMPLVSAISDATLKGFKPDLTLYLDIDPKLGLERAARRGE
LDRIEQQEIEFFKRTRATFLSFADADESIKIVDASQSMAEVHKDIIALLQEQSW

Sequences:

>Translated_214_residues
MNTPVTAAQSAKFIVIEGLEGAGKSSAIALVRDFIEKHTGKAPICTREPGGTPLAEQMRDLVKIANESDPLCDEAECLLF
YAARAQLVANVIKPALANGEWVLGDRHNLSSLAYQGGGRKLMPLVSAISDATLKGFKPDLTLYLDIDPKLGLERAARRGE
LDRIEQQEIEFFKRTRATFLSFADADESIKIVDASQSMAEVHKDIIALLQEQSW
>Mature_214_residues
MNTPVTAAQSAKFIVIEGLEGAGKSSAIALVRDFIEKHTGKAPICTREPGGTPLAEQMRDLVKIANESDPLCDEAECLLF
YAARAQLVANVIKPALANGEWVLGDRHNLSSLAYQGGGRKLMPLVSAISDATLKGFKPDLTLYLDIDPKLGLERAARRGE
LDRIEQQEIEFFKRTRATFLSFADADESIKIVDASQSMAEVHKDIIALLQEQSW

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=194, Percent_Identity=55.1546391752577, Blast_Score=211, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 23412; Mature: 23412

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPVTAAQSAKFIVIEGLEGAGKSSAIALVRDFIEKHTGKAPICTREPGGTPLAEQMRD
CCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
LVKIANESDPLCDEAECLLFYAARAQLVANVIKPALANGEWVLGDRHNLSSLAYQGGGRK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCCCCH
LMPLVSAISDATLKGFKPDLTLYLDIDPKLGLERAARRGELDRIEQQEIEFFKRTRATFL
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
SFADADESIKIVDASQSMAEVHKDIIALLQEQSW
HHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTPVTAAQSAKFIVIEGLEGAGKSSAIALVRDFIEKHTGKAPICTREPGGTPLAEQMRD
CCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
LVKIANESDPLCDEAECLLFYAARAQLVANVIKPALANGEWVLGDRHNLSSLAYQGGGRK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCCCCH
LMPLVSAISDATLKGFKPDLTLYLDIDPKLGLERAARRGELDRIEQQEIEFFKRTRATFL
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
SFADADESIKIVDASQSMAEVHKDIIALLQEQSW
HHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]