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The map label for this gene is fusA2 [H]

Identifier: 157961623

GI number: 157961623

Start: 2195597

End: 2197681

Strand: Reverse

Name: fusA2 [H]

Synonym: Spea_1799

Alternate gene names: 157961623

Gene position: 2197681-2195597 (Counterclockwise)

Preceding gene: 157961624

Following gene: 157961620

Centisome position: 42.47

GC content: 44.41

Gene sequence:

>2085_bases
ATGACTGATTTATCAAAGTACAGAAACATTGGTATCTTTGCTCACGTTGACGCGGGTAAGACTACTACAACTGAGCGTAT
CCTAAAGCTTACTGGTAAAATCCATAAGCTAGGTGAAGTGCATGACGGCGAATCAACAACTGACTTCATGGAACAGGAAG
CTGAGCGTGGTATTACCATTCAGTCTGCTGCTGTTAGTTGTTTCTGGAAAGATCACCGTTTCAACGTTATCGACACCCCT
GGTCACGTTGACTTTACAGTAGAAGTTTACCGTTCTCTTAAAGTTCTTGATGGCGGTATCGGTGTATTCTGTGGTTCTGG
TGGTGTTGAGCCACAATCAGAAACTAACTGGCGTTATGCTAACGAATCTGAAGTTGCTCGTATCATCTTCGTAAACAAGT
TAGACCGTATGGGTGCTGACTTCTTACGTGTTGTTAAGCAAACTAAAGACGTACTAGCTGCTAACCCACTAGTTATGGTA
CTTCCAATCGGTATCGAAGATGAGTTCAGTGGTGTTGTTGACCTACTTTCTCGTAAAGCATACGTATGGGATGATACTGG
TGAAGCTGAAAACTACACAGTAACTGACGTTCCAGCTGACATGGTTGACCAAGTTGAAGAATACCGTGAAATGCTAATCG
AGACAGCTGTTGAGATGGACGACGACCTAATGGAATCTTACATGGAAGGCGAAGAGCCAGCTATGGAAGACATTAAGCGT
TGTATCCGTGCTGGTACTCGTGACATGACTTTCTTCCCAACATACTGTGGTTCTGCTTTCAAGAACAAAGGTATGCAGCT
TCTTCTTGATGCTGTTGTTGATTACCTACCAAACCCAGTTGAAGTTGATCCACAGCCTCTAACTGACGAAGAAGGTAACG
AAAACGGTGAGTTCGCTTTAGTTTCTATCGACGAGCCACTAAAAGCACTAGCATTCAAAATCATGGATGACCGTTTCGGT
GCACTTACATTCGTACGTATCTACTCTGGTCAGCTACGTAAAGGCGACACGATTCTTAACAGTGCAACTGGTAAGACTGA
GCGTATCGGCCGTATGTGTGAAATGCAAGCTGATGAGCGTAACGAACTTGATTACGCACAAGCTGGTGACATCATCGCGA
TCGTTGGTATGAAGAACGTGCAAACTGGTCACACTTTATGTGATGTTAAGCACCCTTGTACTCTTGAAGCAATGGTATTC
CCAGAGCCAGTTATCTCTATCGCAGTAGCGCCAAAAGATAAAGGCGGTTCTGAGAAAATGGGTATCGCTATCGGTAAAAT
GATTGCAGAAGATCCATCTTTCCGCGTAGAAACTGACGAAGACTCAGGCGAAACTATCCTTAAAGGTATGGGTGAACTTC
ACCTAGACATTAAGGTAGACATCCTTAAGCGTACTTACGGCGTTGAGCTAATTGTAGGTGAGCCTCAAGTTGCTTACCGT
GAAACTATCACTCGTGAAATCGAAGATAGCTACACTCACAAGAAGCAATCTGGTGGTTCTGGTCAGTTCGGTAAGATCGA
CTACGTTATCCGTCCAGGCGAAGCTAACACAGGCTTAACGTTCAAATCTTCAGTTGTTGGTGGTAACGTTCCTAAGGAAT
TCTGGCCAGCAGTTGAGAAAGGTTTCGCTAGCATGATGAACACCGGTACTGTTGCTGGTTTCCCTGTGTTAGACGTTGAA
TTCGAACTTAAAGATGGTGCTTTCCACGCAGTTGACTCATCAGCTATCGCGTTCGAAATCGCTGCTAAAGGTGCTTTCCG
TCAATCTATGCCAAAAGCCGGTGCACAACTTCTTGAGCCTATCATGAACGTTGACGTATTCAGCCCAGATGACAACGTTG
GTGACGTAATTGGTGACCTTAACCGTCGTCGTGGTATGATCAAAGACCAAAACGCTGGTGTAACTGGTGTTCGTATTAAG
GCTGACGTACCGCTTTCAGAAATGTTCGGTTACATCGGTTCACTACGTACTATGACTTCTGGTCGTGGCCAATTCTCTAT
GGAATTCGCTCACTACTCACAGTGTCCAAACAGTGTTTCTGAAAAAGTTATCGCTGACGTTAAAGAAAGAAACGCTAAGA
AGTAA

Upstream 100 bases:

>100_bases
GTTTAATTTAGCCCTGAATGATAAAAATCGATAAAATAAATTGAAAAGTATTGCTATACTTGCGCGGAATTTTAGAACGT
AACTCCAATAGAGTAGAAAA

Downstream 100 bases:

>100_bases
CTATTAATTTAGTTATGACTCTTTAGAGTTTTAAACTTTAATAAAAAGCCTATAGATGTAAATCTATAGGCTTTTTTATT
ACTTAAAATTCATCTTAGCT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G 2 [H]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MTDLSKYRNIGIFAHVDAGKTTTTERILKLTGKIHKLGEVHDGESTTDFMEQEAERGITIQSAAVSCFWKDHRFNVIDTP
GHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESEVARIIFVNKLDRMGADFLRVVKQTKDVLAANPLVMV
LPIGIEDEFSGVVDLLSRKAYVWDDTGEAENYTVTDVPADMVDQVEEYREMLIETAVEMDDDLMESYMEGEEPAMEDIKR
CIRAGTRDMTFFPTYCGSAFKNKGMQLLLDAVVDYLPNPVEVDPQPLTDEEGNENGEFALVSIDEPLKALAFKIMDDRFG
ALTFVRIYSGQLRKGDTILNSATGKTERIGRMCEMQADERNELDYAQAGDIIAIVGMKNVQTGHTLCDVKHPCTLEAMVF
PEPVISIAVAPKDKGGSEKMGIAIGKMIAEDPSFRVETDEDSGETILKGMGELHLDIKVDILKRTYGVELIVGEPQVAYR
ETITREIEDSYTHKKQSGGSGQFGKIDYVIRPGEANTGLTFKSSVVGGNVPKEFWPAVEKGFASMMNTGTVAGFPVLDVE
FELKDGAFHAVDSSAIAFEIAAKGAFRQSMPKAGAQLLEPIMNVDVFSPDDNVGDVIGDLNRRRGMIKDQNAGVTGVRIK
ADVPLSEMFGYIGSLRTMTSGRGQFSMEFAHYSQCPNSVSEKVIADVKERNAKK

Sequences:

>Translated_694_residues
MTDLSKYRNIGIFAHVDAGKTTTTERILKLTGKIHKLGEVHDGESTTDFMEQEAERGITIQSAAVSCFWKDHRFNVIDTP
GHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESEVARIIFVNKLDRMGADFLRVVKQTKDVLAANPLVMV
LPIGIEDEFSGVVDLLSRKAYVWDDTGEAENYTVTDVPADMVDQVEEYREMLIETAVEMDDDLMESYMEGEEPAMEDIKR
CIRAGTRDMTFFPTYCGSAFKNKGMQLLLDAVVDYLPNPVEVDPQPLTDEEGNENGEFALVSIDEPLKALAFKIMDDRFG
ALTFVRIYSGQLRKGDTILNSATGKTERIGRMCEMQADERNELDYAQAGDIIAIVGMKNVQTGHTLCDVKHPCTLEAMVF
PEPVISIAVAPKDKGGSEKMGIAIGKMIAEDPSFRVETDEDSGETILKGMGELHLDIKVDILKRTYGVELIVGEPQVAYR
ETITREIEDSYTHKKQSGGSGQFGKIDYVIRPGEANTGLTFKSSVVGGNVPKEFWPAVEKGFASMMNTGTVAGFPVLDVE
FELKDGAFHAVDSSAIAFEIAAKGAFRQSMPKAGAQLLEPIMNVDVFSPDDNVGDVIGDLNRRRGMIKDQNAGVTGVRIK
ADVPLSEMFGYIGSLRTMTSGRGQFSMEFAHYSQCPNSVSEKVIADVKERNAKK
>Mature_693_residues
TDLSKYRNIGIFAHVDAGKTTTTERILKLTGKIHKLGEVHDGESTTDFMEQEAERGITIQSAAVSCFWKDHRFNVIDTPG
HVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESEVARIIFVNKLDRMGADFLRVVKQTKDVLAANPLVMVL
PIGIEDEFSGVVDLLSRKAYVWDDTGEAENYTVTDVPADMVDQVEEYREMLIETAVEMDDDLMESYMEGEEPAMEDIKRC
IRAGTRDMTFFPTYCGSAFKNKGMQLLLDAVVDYLPNPVEVDPQPLTDEEGNENGEFALVSIDEPLKALAFKIMDDRFGA
LTFVRIYSGQLRKGDTILNSATGKTERIGRMCEMQADERNELDYAQAGDIIAIVGMKNVQTGHTLCDVKHPCTLEAMVFP
EPVISIAVAPKDKGGSEKMGIAIGKMIAEDPSFRVETDEDSGETILKGMGELHLDIKVDILKRTYGVELIVGEPQVAYRE
TITREIEDSYTHKKQSGGSGQFGKIDYVIRPGEANTGLTFKSSVVGGNVPKEFWPAVEKGFASMMNTGTVAGFPVLDVEF
ELKDGAFHAVDSSAIAFEIAAKGAFRQSMPKAGAQLLEPIMNVDVFSPDDNVGDVIGDLNRRRGMIKDQNAGVTGVRIKA
DVPLSEMFGYIGSLRTMTSGRGQFSMEFAHYSQCPNSVSEKVIADVKERNAKK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=691, Percent_Identity=43.9942112879884, Blast_Score=560, Evalue=1e-159,
Organism=Homo sapiens, GI19923640, Length=710, Percent_Identity=38.0281690140845, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI25306287, Length=709, Percent_Identity=35.5430183356841, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI25306283, Length=395, Percent_Identity=44.3037974683544, Blast_Score=283, Evalue=5e-76,
Organism=Homo sapiens, GI157426893, Length=159, Percent_Identity=38.3647798742138, Blast_Score=106, Evalue=9e-23,
Organism=Homo sapiens, GI4503483, Length=153, Percent_Identity=36.6013071895425, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=34.5323741007194, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI310132016, Length=122, Percent_Identity=36.0655737704918, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI310110807, Length=122, Percent_Identity=36.0655737704918, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI310123363, Length=122, Percent_Identity=36.0655737704918, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1789738, Length=695, Percent_Identity=53.2374100719424, Blast_Score=737, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=508, Percent_Identity=25, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1788922, Length=154, Percent_Identity=36.3636363636364, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI48994988, Length=175, Percent_Identity=35.4285714285714, Blast_Score=96, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=697, Percent_Identity=38.8809182209469, Blast_Score=483, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17556745, Length=713, Percent_Identity=29.7335203366059, Blast_Score=295, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI17557151, Length=151, Percent_Identity=39.0728476821192, Blast_Score=107, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17506493, Length=187, Percent_Identity=34.2245989304813, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17552882, Length=135, Percent_Identity=32.5925925925926, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=142, Percent_Identity=30.2816901408451, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=142, Percent_Identity=30.2816901408451, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=698, Percent_Identity=41.404011461318, Blast_Score=549, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6322359, Length=790, Percent_Identity=31.2658227848101, Blast_Score=346, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6323320, Length=158, Percent_Identity=38.6075949367089, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6324707, Length=498, Percent_Identity=23.8955823293173, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320593, Length=498, Percent_Identity=23.8955823293173, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=158, Percent_Identity=32.2784810126582, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=135, Percent_Identity=34.8148148148148, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582462, Length=694, Percent_Identity=41.3544668587896, Blast_Score=539, Evalue=1e-153,
Organism=Drosophila melanogaster, GI221458488, Length=731, Percent_Identity=33.2421340629275, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24585709, Length=837, Percent_Identity=25.2090800477897, Blast_Score=155, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24585711, Length=837, Percent_Identity=25.2090800477897, Blast_Score=155, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24585713, Length=837, Percent_Identity=25.2090800477897, Blast_Score=155, Evalue=1e-37,
Organism=Drosophila melanogaster, GI78706572, Length=155, Percent_Identity=36.7741935483871, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574573, Length=143, Percent_Identity=35.6643356643357, Blast_Score=87, Evalue=5e-17,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76437; Mature: 76305

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLSKYRNIGIFAHVDAGKTTTTERILKLTGKIHKLGEVHDGESTTDFMEQEAERGITI
CCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEE
QSAAVSCFWKDHRFNVIDTPGHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYA
EHHHEEEEEECCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
NESEVARIIFVNKLDRMGADFLRVVKQTKDVLAANPLVMVLPIGIEDEFSGVVDLLSRKA
CCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCC
YVWDDTGEAENYTVTDVPADMVDQVEEYREMLIETAVEMDDDLMESYMEGEEPAMEDIKR
EEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHH
CIRAGTRDMTFFPTYCGSAFKNKGMQLLLDAVVDYLPNPVEVDPQPLTDEEGNENGEFAL
HHHCCCCCCEEEHHHHCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
VSIDEPLKALAFKIMDDRFGALTFVRIYSGQLRKGDTILNSATGKTERIGRMCEMQADER
EECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCCC
NELDYAQAGDIIAIVGMKNVQTGHTLCDVKHPCTLEAMVFPEPVISIAVAPKDKGGSEKM
CCCCHHHCCCEEEEEECCCCCCCCCEECCCCCCEEEEEECCCCEEEEEECCCCCCCCCCH
GIAIGKMIAEDPSFRVETDEDSGETILKGMGELHLDIKVDILKRTYGVELIVGEPQVAYR
HHHHHHHHHCCCCEEEECCCCCCHHHHHCCCCEEEEEEEEEEHHHCCEEEEECCCCHHHH
ETITREIEDSYTHKKQSGGSGQFGKIDYVIRPGEANTGLTFKSSVVGGNVPKEFWPAVEK
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHH
GFASMMNTGTVAGFPVLDVEFELKDGAFHAVDSSAIAFEIAAKGAFRQSMPKAGAQLLEP
HHHHHHCCCCCCCCEEEEEEEEECCCCEEEECCCCEEEEEHHCCHHHHHCCHHHHHHHHH
IMNVDVFSPDDNVGDVIGDLNRRRGMIKDQNAGVTGVRIKADVPLSEMFGYIGSLRTMTS
HHCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCC
GRGQFSMEFAHYSQCPNSVSEKVIADVKERNAKK
CCCCEEEEHHHHHCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDLSKYRNIGIFAHVDAGKTTTTERILKLTGKIHKLGEVHDGESTTDFMEQEAERGITI
CCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEE
QSAAVSCFWKDHRFNVIDTPGHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYA
EHHHEEEEEECCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
NESEVARIIFVNKLDRMGADFLRVVKQTKDVLAANPLVMVLPIGIEDEFSGVVDLLSRKA
CCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCC
YVWDDTGEAENYTVTDVPADMVDQVEEYREMLIETAVEMDDDLMESYMEGEEPAMEDIKR
EEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHH
CIRAGTRDMTFFPTYCGSAFKNKGMQLLLDAVVDYLPNPVEVDPQPLTDEEGNENGEFAL
HHHCCCCCCEEEHHHHCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
VSIDEPLKALAFKIMDDRFGALTFVRIYSGQLRKGDTILNSATGKTERIGRMCEMQADER
EECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCCC
NELDYAQAGDIIAIVGMKNVQTGHTLCDVKHPCTLEAMVFPEPVISIAVAPKDKGGSEKM
CCCCHHHCCCEEEEEECCCCCCCCCEECCCCCCEEEEEECCCCEEEEEECCCCCCCCCCH
GIAIGKMIAEDPSFRVETDEDSGETILKGMGELHLDIKVDILKRTYGVELIVGEPQVAYR
HHHHHHHHHCCCCEEEECCCCCCHHHHHCCCCEEEEEEEEEEHHHCCEEEEECCCCHHHH
ETITREIEDSYTHKKQSGGSGQFGKIDYVIRPGEANTGLTFKSSVVGGNVPKEFWPAVEK
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHH
GFASMMNTGTVAGFPVLDVEFELKDGAFHAVDSSAIAFEIAAKGAFRQSMPKAGAQLLEP
HHHHHHCCCCCCCCEEEEEEEEECCCCEEEECCCCEEEEEHHCCHHHHHCCHHHHHHHHH
IMNVDVFSPDDNVGDVIGDLNRRRGMIKDQNAGVTGVRIKADVPLSEMFGYIGSLRTMTS
HHCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCC
GRGQFSMEFAHYSQCPNSVSEKVIADVKERNAKK
CCCCEEEEHHHHHCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA