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The map label for this gene is bioB [H]

Identifier: 157961624

GI number: 157961624

Start: 2198094

End: 2199434

Strand: Reverse

Name: bioB [H]

Synonym: Spea_1800

Alternate gene names: 157961624

Gene position: 2199434-2198094 (Counterclockwise)

Preceding gene: 157961647

Following gene: 157961623

Centisome position: 42.5

GC content: 46.09

Gene sequence:

>1341_bases
ATGAGCAGCGATAATCAATCTACAACAACAGGTATCGATCTTCAATTTGATAGAGACCACCTATGGCATCCCTACACTTC
AATGGCCAATGCGCTTCCCACCTATGGCGTTGTATCTGCAGAAGGTGTTGAACTCCAGCTAGAAGACGGCACAAAGCTCA
TTGACGGTACCAGTTCATGGTGGGCTTGTGTTCATGGTTATAGTCACCCAAAAATCGTTGCTGCTATGCAACAACAGACT
GCGCAATTGAGTCATGTGATGTTTGGCGGAATAACTCACCGCCCTGCCGTTGAACTTGCGCGTCTACTTATCGAAATGAC
AAGCCCTAGGCTGACTAAGGTTTTTTTAGCCGACTCTGGTTCAATTGCGGTTGAAGTCGCGATGAAGATGGCGCTGCAAT
ACTGGCAAGGTAAGGCTAAACCTAATAAACAGAAGATCCTGACTGTTAAGAATGGCTACCATGGCGATACTTTTGCCGCC
ATGAGTGTATGCGATCCTGAAGGCGGCATGCACACCATGTTCGGTGAGTTAGTGACTAAACAGCTGTTTGCCCCTGCGCC
TCAATCTCGTTTCGATGACGACTTTAACCCAAAAGAACTCAGCGAGATTGACAACCTATTTGAAGATAATCACCAACAGA
TCGCCGCATTGCTCATTGAGCCTATTATGCAAGGCGCTGGCGGTATGCGTTTCTATCATCCAGAATACTTACGCTCGCTA
CGTAAACTGTGTGATAAATATGACGTGCTGCTTATTCTCGATGAAATTGCCACCGGCTTTGGCCGAACAGGTAAATTGTT
CGCCTATGAACACAGCGGGATTGAAGCTGATATTTTATGCTTAGGTAAGGCATTAACCGGTGGCTATATCTCTCTGGCCG
CAACCATATGTAGCAACGAAGTAGCCCAAGGAGTGAGTGACTCGCCAGCAGGAGTGTTTATGCATGGTCCTACCTTTATG
GGTAATCCACTCGCCTGCTCTGCTGCTATCGCCAGTTTAGAGCTGCTTAAAGAAAATCGTTGGCAAGGACAGGTTCGCGC
TATTGAATCTCAGCTCAAGCAGGAATTAGCCGACGCGATAGACTACCCAAATGTAAAAGATGTTCGTGTATTAGGCAGCA
TTGGTGTGCTCGAAATGAATGCTACCCTCAACACCGCTGAGCTACAGCAGCAGTTCGTCGATCTAGGCGTTTGGATAAGA
CCATTTTCTAACCTTATCTATATCATGCCGCCATATTGCATAAGTTCATCTCAGCTAAGCAAATTAACCCATGCAATGAA
AACTGTGGCAAAACAGATCAGTTTACCCAATGATACCAGTGCTTTTATCAGTCATGGTTAA

Upstream 100 bases:

>100_bases
TCAGCTAATTGGTTTTGCAAATATTTATCTTGCCTAGGATACCTGCAAGCCGTAACCTGTCAATGTCACATGAATCAAAA
GGTTTACTAACGGTCAATAT

Downstream 100 bases:

>100_bases
CTGGTAAAGACATTCCTTTCTGTACTTTAATTCTCTGCCCGGCCAATGACGGTAAAACTTACCTAGGTCGGGCAGCCTTG
CCTAATGAAGCAATTCGCAC

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSSDNQSTTTGIDLQFDRDHLWHPYTSMANALPTYGVVSAEGVELQLEDGTKLIDGTSSWWACVHGYSHPKIVAAMQQQT
AQLSHVMFGGITHRPAVELARLLIEMTSPRLTKVFLADSGSIAVEVAMKMALQYWQGKAKPNKQKILTVKNGYHGDTFAA
MSVCDPEGGMHTMFGELVTKQLFAPAPQSRFDDDFNPKELSEIDNLFEDNHQQIAALLIEPIMQGAGGMRFYHPEYLRSL
RKLCDKYDVLLILDEIATGFGRTGKLFAYEHSGIEADILCLGKALTGGYISLAATICSNEVAQGVSDSPAGVFMHGPTFM
GNPLACSAAIASLELLKENRWQGQVRAIESQLKQELADAIDYPNVKDVRVLGSIGVLEMNATLNTAELQQQFVDLGVWIR
PFSNLIYIMPPYCISSSQLSKLTHAMKTVAKQISLPNDTSAFISHG

Sequences:

>Translated_446_residues
MSSDNQSTTTGIDLQFDRDHLWHPYTSMANALPTYGVVSAEGVELQLEDGTKLIDGTSSWWACVHGYSHPKIVAAMQQQT
AQLSHVMFGGITHRPAVELARLLIEMTSPRLTKVFLADSGSIAVEVAMKMALQYWQGKAKPNKQKILTVKNGYHGDTFAA
MSVCDPEGGMHTMFGELVTKQLFAPAPQSRFDDDFNPKELSEIDNLFEDNHQQIAALLIEPIMQGAGGMRFYHPEYLRSL
RKLCDKYDVLLILDEIATGFGRTGKLFAYEHSGIEADILCLGKALTGGYISLAATICSNEVAQGVSDSPAGVFMHGPTFM
GNPLACSAAIASLELLKENRWQGQVRAIESQLKQELADAIDYPNVKDVRVLGSIGVLEMNATLNTAELQQQFVDLGVWIR
PFSNLIYIMPPYCISSSQLSKLTHAMKTVAKQISLPNDTSAFISHG
>Mature_445_residues
SSDNQSTTTGIDLQFDRDHLWHPYTSMANALPTYGVVSAEGVELQLEDGTKLIDGTSSWWACVHGYSHPKIVAAMQQQTA
QLSHVMFGGITHRPAVELARLLIEMTSPRLTKVFLADSGSIAVEVAMKMALQYWQGKAKPNKQKILTVKNGYHGDTFAAM
SVCDPEGGMHTMFGELVTKQLFAPAPQSRFDDDFNPKELSEIDNLFEDNHQQIAALLIEPIMQGAGGMRFYHPEYLRSLR
KLCDKYDVLLILDEIATGFGRTGKLFAYEHSGIEADILCLGKALTGGYISLAATICSNEVAQGVSDSPAGVFMHGPTFMG
NPLACSAAIASLELLKENRWQGQVRAIESQLKQELADAIDYPNVKDVRVLGSIGVLEMNATLNTAELQQQFVDLGVWIRP
FSNLIYIMPPYCISSSQLSKLTHAMKTVAKQISLPNDTSAFISHG

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=419, Percent_Identity=28.1622911694511, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI284507298, Length=312, Percent_Identity=29.1666666666667, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI13994255, Length=433, Percent_Identity=27.7136258660508, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI37574042, Length=438, Percent_Identity=24.2009132420091, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI226442705, Length=415, Percent_Identity=24.0963855421687, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI226442709, Length=409, Percent_Identity=24.6943765281174, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI24119277, Length=423, Percent_Identity=25.2955082742317, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI188536080, Length=291, Percent_Identity=23.3676975945017, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI38679950, Length=291, Percent_Identity=23.3676975945017, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI38679946, Length=291, Percent_Identity=23.3676975945017, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1786991, Length=419, Percent_Identity=59.6658711217184, Blast_Score=513, Evalue=1e-147,
Organism=Escherichia coli, GI1789016, Length=393, Percent_Identity=30.5343511450382, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789759, Length=383, Percent_Identity=31.331592689295, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI145693181, Length=336, Percent_Identity=32.7380952380952, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788044, Length=388, Percent_Identity=30.1546391752577, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1787560, Length=411, Percent_Identity=27.9805352798054, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786349, Length=402, Percent_Identity=26.1194029850746, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=415, Percent_Identity=28.1927710843373, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI25144274, Length=284, Percent_Identity=30.2816901408451, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564660, Length=424, Percent_Identity=28.0660377358491, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71992977, Length=422, Percent_Identity=23.9336492890995, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324386, Length=456, Percent_Identity=44.9561403508772, Blast_Score=411, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6323470, Length=412, Percent_Identity=29.8543689320388, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6324432, Length=333, Percent_Identity=32.4324324324324, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321456, Length=306, Percent_Identity=25.4901960784314, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21357415, Length=414, Percent_Identity=29.7101449275362, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI28574759, Length=393, Percent_Identity=26.4631043256997, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI161085790, Length=397, Percent_Identity=25.6926952141058, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356575, Length=410, Percent_Identity=23.6585365853659, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281366494, Length=289, Percent_Identity=26.9896193771626, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24667139, Length=289, Percent_Identity=26.9896193771626, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24667143, Length=289, Percent_Identity=26.9896193771626, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 48937; Mature: 48805

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDNQSTTTGIDLQFDRDHLWHPYTSMANALPTYGVVSAEGVELQLEDGTKLIDGTSSW
CCCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCEECCCCEEEECCCCEEECCCCHH
WACVHGYSHPKIVAAMQQQTAQLSHVMFGGITHRPAVELARLLIEMTSPRLTKVFLADSG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCC
SIAVEVAMKMALQYWQGKAKPNKQKILTVKNGYHGDTFAAMSVCDPEGGMHTMFGELVTK
CHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHH
QLFAPAPQSRFDDDFNPKELSEIDNLFEDNHQQIAALLIEPIMQGAGGMRFYHPEYLRSL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHH
RKLCDKYDVLLILDEIATGFGRTGKLFAYEHSGIEADILCLGKALTGGYISLAATICSNE
HHHHHHCCCEEEEHHHHHCCCCCCCEEEEECCCCCEEHEEECHHHCCCHHHHHHHHHHHH
VAQGVSDSPAGVFMHGPTFMGNPLACSAAIASLELLKENRWQGQVRAIESQLKQELADAI
HHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
DYPNVKDVRVLGSIGVLEMNATLNTAELQQQFVDLGVWIRPFSNLIYIMPPYCISSSQLS
CCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
KLTHAMKTVAKQISLPNDTSAFISHG
HHHHHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure 
SSDNQSTTTGIDLQFDRDHLWHPYTSMANALPTYGVVSAEGVELQLEDGTKLIDGTSSW
CCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCEECCCCEEEECCCCEEECCCCHH
WACVHGYSHPKIVAAMQQQTAQLSHVMFGGITHRPAVELARLLIEMTSPRLTKVFLADSG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCC
SIAVEVAMKMALQYWQGKAKPNKQKILTVKNGYHGDTFAAMSVCDPEGGMHTMFGELVTK
CHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHH
QLFAPAPQSRFDDDFNPKELSEIDNLFEDNHQQIAALLIEPIMQGAGGMRFYHPEYLRSL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHH
RKLCDKYDVLLILDEIATGFGRTGKLFAYEHSGIEADILCLGKALTGGYISLAATICSNE
HHHHHHCCCEEEEHHHHHCCCCCCCEEEEECCCCCEEHEEECHHHCCCHHHHHHHHHHHH
VAQGVSDSPAGVFMHGPTFMGNPLACSAAIASLELLKENRWQGQVRAIESQLKQELADAI
HHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
DYPNVKDVRVLGSIGVLEMNATLNTAELQQQFVDLGVWIRPFSNLIYIMPPYCISSSQLS
CCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
KLTHAMKTVAKQISLPNDTSAFISHG
HHHHHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA