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The map label for this gene is topB [H]

Identifier: 157961451

GI number: 157961451

Start: 1965468

End: 1967486

Strand: Direct

Name: topB [H]

Synonym: Spea_1625

Alternate gene names: 157961451

Gene position: 1965468-1967486 (Clockwise)

Preceding gene: 157961447

Following gene: 157961452

Centisome position: 37.98

GC content: 47.99

Gene sequence:

>2019_bases
ATGATCTTATATATTGCTGAAAAACCCAGCCTTGGTAGAGCTATTGCCGATGTGTTGCCTAAACCCTTAAAGAAAGGTGA
TGGCTTTATTCGCGCCGCAAATGGCGATTGTGTCTCTTGGTGTGTCGGTCATTTACTCGAACAGGCTGAACCAGATGCAT
ACTCACCAGAGTACAAGTCATGGAAGTTCGAGCATCTGCCAATCGTGCCAGAGACCTGGAAGATGAAGCCTAAATATAAG
ATGCGCGGCCAGATAGCGGTGCTGCGAGGCTTAGTCAAAGAGGCGAGCCAGCTCGTAAATGCCGGCGACCCGGATAGAGA
AGGGCAGCTATTGGTCGATGAGATCATTGCCCACTTAGGCGTAAAAGGCCCAAAATTGCAAAACGCGCAGCGTCTACTCA
TCAGTGACTTGAATCCACAAGCGGTGAAAAGGGCATTAGGGCAGTTACGCTCTAACCGCGAGTTTATTCCTCTGTCGACC
TCAGCCTTAGCGCGCTCTCGCGCCGATTGGCTCTATGGCATGAATATGACCCGGGCTTACACCATTCAAGGGCGCAAAGT
CGGCTATCAAGGCGTGCTGTCTGTTGGGCGGGTGCAAACTCCTGTGCTGGGCTTAGTGGTTCGCCGCGACGCCGAAATTG
CCGAGTTTATCTCTAAACCTTTCTATGAGGTGTTGGCGCATTTAGCGACCGATAATCAGCAGCGATTTAGTGCAAAATGG
CAACCAAGTGAGGCCTGCCAGCCTTATATGGATGAAGAGGGGCGAGTACTCTCAAAAGGGTTAGCGCAAAACGTAGTCGG
GCGTATAAATGGCCAAAACGGCACCGTAGAAAAGGTAACAGCACAAGATAAGAAACAAAATGCACCTTTGCCTTATAGCT
TATCGGCACTGCAGATTGATGCGGCTAAACGCTTCGGTATGAGTGCAAAGTCAGTACTCGATACCTGCCAGTCTTTATAT
GAAAAACATAAGCTTATCACCTATCCCCGTTCAGATAGCCGTTATCTACCTGCTGAACAACATGCTTTGGCTCCGAGCGT
CATAGGTGCCGTACTGAATGGTGCCAGTGAGTTAGTGTCAGGCATAGAGCAACCTAATAGCAAGCTTAAATCTAAGGCGT
GGAATGATAAAAAGGTTGAGGCTCACCACGCGATAGTGCCGACCGAGAAGACCGCTAATTTACAAAACTTAAATCAGGCT
GAGAAGCAGTTGTATCTACAGGTTGCTCGTCAGTATTTGATGCAATTTTATCCTGCGTATCTTTATAAAGAGACTCAAGT
TGAGATAATCATTGCAGGTGGCTTATTTAAAACCAAGGCCAAACAAGAGGTGGCATTGGGTTGGAAGCAACTGCTTAAGC
AAAATAAAGAGAAGAGCCAAAGCGAAAAGCAACAAACTGATGATGACGAGTATCTAGCCAACATTCCCGCCCTAAAAAAG
GGCCAAATTCTGTTATGTGAGCGCGGTGAGTTACTGGAGAAACAGACTCAGCCACCAAAGCACTTTAGCGATGCCACTTT
ACTGGGCGCGATGACAGGGATCAGCCGTTATGTGACCGACAGTGAGATCCGCAAAATATTAAAGGAGACTGATGGGCTCG
GTACCGAGGCGACTCGCGCTGGGATCATCGAGTTGTTGTTTAAGCGAAACTACCTAACGCGTCAAGGTAAGTCTATTTTA
GCGACAGAGGTAGGCAAGGGTTTGATAAACAGCTTACCCCTGAGTGCGACCACACCGGACATGACGGCACTTTGGGAAAA
TAATCTCGATGCTATTAGTCGTAAAGAGGCTAAATATCAAAGCTTTATGGCGCCTTTATTAATATCACTGCAGCAGCTTA
TCGAGCAGGCTGGGGCGCAGTTACCCACCTCGTTGCAGGGCGTTAAATCTCCTGATAGCGGCTTTAAAAAGCGTCGTTAC
TCGGGCAATAAGAGTGCGGCTACGACAAAATCGGCTACAGCTAAGAAAGCGTCAGCTAAAAGAGTGAGTACGACTAAGGC
TAAGAGAGCGACCTCCTAA

Upstream 100 bases:

>100_bases
GCAGCGAATAATGCCTTTTGAGGGACGGCTTTCATTTGCACCATTTAGCGCTTCGATGTACAGTTACTGTTAATTTATAC
AGTAATCCTAATTTTTCTAA

Downstream 100 bases:

>100_bases
ATCATCAGCTTGAGTTCTTGTTTAGTAACTTTCAATTGTTTAACAAAGGCCTATGGGGTAGCTTGGTATCTAAAGCAACT
ATGGAGTGAGACAGATGTTT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MILYIAEKPSLGRAIADVLPKPLKKGDGFIRAANGDCVSWCVGHLLEQAEPDAYSPEYKSWKFEHLPIVPETWKMKPKYK
MRGQIAVLRGLVKEASQLVNAGDPDREGQLLVDEIIAHLGVKGPKLQNAQRLLISDLNPQAVKRALGQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGRKVGYQGVLSVGRVQTPVLGLVVRRDAEIAEFISKPFYEVLAHLATDNQQRFSAKW
QPSEACQPYMDEEGRVLSKGLAQNVVGRINGQNGTVEKVTAQDKKQNAPLPYSLSALQIDAAKRFGMSAKSVLDTCQSLY
EKHKLITYPRSDSRYLPAEQHALAPSVIGAVLNGASELVSGIEQPNSKLKSKAWNDKKVEAHHAIVPTEKTANLQNLNQA
EKQLYLQVARQYLMQFYPAYLYKETQVEIIIAGGLFKTKAKQEVALGWKQLLKQNKEKSQSEKQQTDDDEYLANIPALKK
GQILLCERGELLEKQTQPPKHFSDATLLGAMTGISRYVTDSEIRKILKETDGLGTEATRAGIIELLFKRNYLTRQGKSIL
ATEVGKGLINSLPLSATTPDMTALWENNLDAISRKEAKYQSFMAPLLISLQQLIEQAGAQLPTSLQGVKSPDSGFKKRRY
SGNKSAATTKSATAKKASAKRVSTTKAKRATS

Sequences:

>Translated_672_residues
MILYIAEKPSLGRAIADVLPKPLKKGDGFIRAANGDCVSWCVGHLLEQAEPDAYSPEYKSWKFEHLPIVPETWKMKPKYK
MRGQIAVLRGLVKEASQLVNAGDPDREGQLLVDEIIAHLGVKGPKLQNAQRLLISDLNPQAVKRALGQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGRKVGYQGVLSVGRVQTPVLGLVVRRDAEIAEFISKPFYEVLAHLATDNQQRFSAKW
QPSEACQPYMDEEGRVLSKGLAQNVVGRINGQNGTVEKVTAQDKKQNAPLPYSLSALQIDAAKRFGMSAKSVLDTCQSLY
EKHKLITYPRSDSRYLPAEQHALAPSVIGAVLNGASELVSGIEQPNSKLKSKAWNDKKVEAHHAIVPTEKTANLQNLNQA
EKQLYLQVARQYLMQFYPAYLYKETQVEIIIAGGLFKTKAKQEVALGWKQLLKQNKEKSQSEKQQTDDDEYLANIPALKK
GQILLCERGELLEKQTQPPKHFSDATLLGAMTGISRYVTDSEIRKILKETDGLGTEATRAGIIELLFKRNYLTRQGKSIL
ATEVGKGLINSLPLSATTPDMTALWENNLDAISRKEAKYQSFMAPLLISLQQLIEQAGAQLPTSLQGVKSPDSGFKKRRY
SGNKSAATTKSATAKKASAKRVSTTKAKRATS
>Mature_672_residues
MILYIAEKPSLGRAIADVLPKPLKKGDGFIRAANGDCVSWCVGHLLEQAEPDAYSPEYKSWKFEHLPIVPETWKMKPKYK
MRGQIAVLRGLVKEASQLVNAGDPDREGQLLVDEIIAHLGVKGPKLQNAQRLLISDLNPQAVKRALGQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGRKVGYQGVLSVGRVQTPVLGLVVRRDAEIAEFISKPFYEVLAHLATDNQQRFSAKW
QPSEACQPYMDEEGRVLSKGLAQNVVGRINGQNGTVEKVTAQDKKQNAPLPYSLSALQIDAAKRFGMSAKSVLDTCQSLY
EKHKLITYPRSDSRYLPAEQHALAPSVIGAVLNGASELVSGIEQPNSKLKSKAWNDKKVEAHHAIVPTEKTANLQNLNQA
EKQLYLQVARQYLMQFYPAYLYKETQVEIIIAGGLFKTKAKQEVALGWKQLLKQNKEKSQSEKQQTDDDEYLANIPALKK
GQILLCERGELLEKQTQPPKHFSDATLLGAMTGISRYVTDSEIRKILKETDGLGTEATRAGIIELLFKRNYLTRQGKSIL
ATEVGKGLINSLPLSATTPDMTALWENNLDAISRKEAKYQSFMAPLLISLQQLIEQAGAQLPTSLQGVKSPDSGFKKRRY
SGNKSAATTKSATAKKASAKRVSTTKAKRATS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1788061, Length=659, Percent_Identity=55.2352048558422, Blast_Score=743, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=595, Percent_Identity=24.3697478991597, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32563869, Length=649, Percent_Identity=24.4992295839753, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 74428; Mature: 74428

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILYIAEKPSLGRAIADVLPKPLKKGDGFIRAANGDCVSWCVGHLLEQAEPDAYSPEYKS
CEEEEECCCCHHHHHHHHCCHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCC
WKFEHLPIVPETWKMKPKYKMRGQIAVLRGLVKEASQLVNAGDPDREGQLLVDEIIAHLG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
VKGPKLQNAQRLLISDLNPQAVKRALGQLRSNREFIPLSTSALARSRADWLYGMNMTRAY
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEE
TIQGRKVGYQGVLSVGRVQTPVLGLVVRRDAEIAEFISKPFYEVLAHLATDNQQRFSAKW
EECCCCCCHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
QPSEACQPYMDEEGRVLSKGLAQNVVGRINGQNGTVEKVTAQDKKQNAPLPYSLSALQID
CCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHCCHHHCCCCCCCCCCHHHEEH
AAKRFGMSAKSVLDTCQSLYEKHKLITYPRSDSRYLPAEQHALAPSVIGAVLNGASELVS
HHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHH
GIEQPNSKLKSKAWNDKKVEAHHAIVPTEKTANLQNLNQAEKQLYLQVARQYLMQFYPAY
HHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYKETQVEIIIAGGLFKTKAKQEVALGWKQLLKQNKEKSQSEKQQTDDDEYLANIPALKK
HCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCC
GQILLCERGELLEKQTQPPKHFSDATLLGAMTGISRYVTDSEIRKILKETDGLGTEATRA
CCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHH
GIIELLFKRNYLTRQGKSILATEVGKGLINSLPLSATTPDMTALWENNLDAISRKEAKYQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHH
SFMAPLLISLQQLIEQAGAQLPTSLQGVKSPDSGFKKRRYSGNKSAATTKSATAKKASAK
HHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHH
RVSTTKAKRATS
HHHHHHHHHCCC
>Mature Secondary Structure
MILYIAEKPSLGRAIADVLPKPLKKGDGFIRAANGDCVSWCVGHLLEQAEPDAYSPEYKS
CEEEEECCCCHHHHHHHHCCHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCC
WKFEHLPIVPETWKMKPKYKMRGQIAVLRGLVKEASQLVNAGDPDREGQLLVDEIIAHLG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
VKGPKLQNAQRLLISDLNPQAVKRALGQLRSNREFIPLSTSALARSRADWLYGMNMTRAY
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEE
TIQGRKVGYQGVLSVGRVQTPVLGLVVRRDAEIAEFISKPFYEVLAHLATDNQQRFSAKW
EECCCCCCHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
QPSEACQPYMDEEGRVLSKGLAQNVVGRINGQNGTVEKVTAQDKKQNAPLPYSLSALQID
CCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHCCHHHCCCCCCCCCCHHHEEH
AAKRFGMSAKSVLDTCQSLYEKHKLITYPRSDSRYLPAEQHALAPSVIGAVLNGASELVS
HHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHH
GIEQPNSKLKSKAWNDKKVEAHHAIVPTEKTANLQNLNQAEKQLYLQVARQYLMQFYPAY
HHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYKETQVEIIIAGGLFKTKAKQEVALGWKQLLKQNKEKSQSEKQQTDDDEYLANIPALKK
HCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCC
GQILLCERGELLEKQTQPPKHFSDATLLGAMTGISRYVTDSEIRKILKETDGLGTEATRA
CCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHH
GIIELLFKRNYLTRQGKSILATEVGKGLINSLPLSATTPDMTALWENNLDAISRKEAKYQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHH
SFMAPLLISLQQLIEQAGAQLPTSLQGVKSPDSGFKKRRYSGNKSAATTKSATAKKASAK
HHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHH
RVSTTKAKRATS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]